Thermo Attune CytPix Acoustic Focusing Cytometer (3)Lasers/(11) Colors/(13)Detectors w/ CytKick Max Autosampler

For Sale Thermo Attune CytPix Imaging Cytometer (3)Lasers/(11) Colors/(13)Detectors w/ CytKick Max Autosampler

This item has been recently sold. We often have more on the way, if you are current customer or a new customer and looking for one more Thermo Attune CytPix instrument for your lab, please send us an inquiry and we will work on getting one ready for you. Please let us know via the “Request Quote” form; we replenish sold-out items often.

Average restock time frame 2-6 weeks.

Please also view the other similar instruments on your right (with arrows) or click on all categories to see what we have available. Thank you! 

• Fully Functional
• Serviced & in Stock
• Expert sales consultation
•  Includes remote installation and an instrument training session
•  Includes subscription to De Novo FCS Express 7 Analysis software
•  Warranty

This is the Thermo Attune Cytpix Cytometer that includes additional brightfield imaging capabilities

Here is what you will receive:

• Thermo Attune CytPix Flow Cytometer
• CytKick Max Autosampler
• PC running Attune software w/ monitor, keyboard, and mouse
• Complimentary De Novo FCS Express analysis software (1-Year Subscription) RUO version
• Communication and power cables

When you purchase a flow cytometer from Boston Industries, you also receive a complimentary 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

• User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
• Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
• Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
• High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
• Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
• With FCS Express 7, you will have the latest Research Use analysis software.

Thermo Attune CytPix Flow Cytometer Configuration:

Optics:(3) Lasers, (11) Colors, (13) Detectors

• Forward Scatter Channel: 488/10 blue laser
• Side Scatter Channel: 488/10 blue laser
• 405nm violet laser: 440/50, 512/25, 603/48, 710/50
• 488nm blue laser: 530/30, 574/26, 695/40, 780/60
• 637nm red laser: 670/14, 720/30, 780/60
• Laser Color Allocation (Laser/Channel Count): Violet/4, Blue/4, and Red/3

• Flow Rate: 12.5 to 1,000 uL/min
• Sample Analysis Volume: 20 uL to 4 mL
• Custom sample flow rates 12.5–1,000 uL/min
• Sample delivery: positive displacement syringe pump for volumetric analysis
• Sample tubes: accommodates 17 x 100 mm to 8.5 x 45 mm tubes
• Data Acquisition Speed: Up to 35,000 events/sec with all parameters
• Maximum Electronic Speed: 65,000 events/second with all parameters
• Fluorescence sensitivity: ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APC
• Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
• Performance imaging:
  • Pixel resolution 0.3 μm/pixel
  • Objective magnification 20x
  • Visually detect 800 nm particles
  • Image capture rate Up to 6,000 images/second, depending on image size and event rate
  • Image size 96 x 96 pixels to 248 x 248 pixels
  • Field of view 29 x 29 μm2 to 74 x 74 μm2

CytKick MAX Autosampler Configuration:

• Minimum Sample Volume: 50 uL (70 uL at 1000 uL/minute)
• Dead Volume: 30 uL (except 50 uL at 500–1000 uL/min)
• Minimum Sample Acquisition Volume: 20 uL
• Throughput: 22 min for 96-well plate (boost mode, using one rinse and one mix and full analysis of a 20 µL sample)
• Carryover: <0.5% in standard mode using 2 wash cycles; <1% in Boost Mode
• Compatibility: 
  • 96 deep-well
  • 96-well standard depth
  • 384 deep-well
  • 384-well standard depth
  • 1.5 mL and 2 mL microcentrifuge tube rack (up to 24 per rack)
  • 96-well foil-covered
  • 384-well foil-covered customizable to accept additional plate types
• Cooling: passive cooling available for 96-well U-bottom plates and microcentrifuge tube racks

Customization/Options:

• Orbitior RS2 Microplate Mover  (Will require Thermo Momentum scheduling software)
• Attune NxT Extended Fluid System EFS1 for extended operation
• Other filter colors may be available (Please inquire for filter options in stock)

Testing performed by our in-house service team:

The Thermo Attune CytPix Flow Cytometer passed all tests and was found to be fully functional:

• We check the startup and initialization process.
  
• We verify the communication between the instrument and the software.
• We run the fluidics priming and startup sequences.
• We conduct an extensive cleaning of the fluidic system.
• We perform baseline and performance tests using Attune Performance Tracking Beads.
• We test the image view option and confirm single event image capture.
• We test the full functionality of the CytKick Max autosampler.
• We perform a final QC and a PM before prepping for shipping.

Instrument Overview:

• The Thermo Attune CytPix uses acoustic focusing for precise and rapid cell alignment during analysis.
• It features 3 lasers and supports detection of up to 11 fluorescent colors simultaneously.
• The image view option allows an overview of the images, along with a cell image option to view individual images in the workspace for any cell population.
• Includes the CytKick Max autosampler for automated high-throughput sample loading.
• Thermo Attune CytPix enables reliable data acquisition with consistent fluidics control.
• By backgating all or only selected images onto supported workspace plots, you can correlate images to flow cytometry data.

Instrument Application:

• High-throughput immunophenotyping
• Rare cell detection
• Cell cycle analysis
• Multiparameter fluorescent profiling
• Apoptosis and viability assays
• Antigen-specific T-cell analysis

The key features:

• Acoustic focusing for precise cell alignment
• Cell image option to view individual events
• 3 lasers for flexible excitation options
• Detects up to 11 fluorescent colors
• 13 detectors for high-resolution data
• CytKick Max enables automated sampling

 De Novo FCS Express Software Features

• Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
• Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
• Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
• Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
• Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
• Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
• Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
• Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

 Benefits of the Complimentary DeNovo FCS Express Software

• Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
• User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
• Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
• Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
• Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
• Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
• Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
• Seamless Integration: Integration capabilities with other software and databases ensure a smooth workflow and data management.

Additional technical info:

• Analysis Software: FCS Express Analysis Software
• Manual: Thermo Attune CytPix Flow Cytometer Manual
• Manual: CytKick MAX Autosampler Manual

Note: Subscription is for the Research Use Only (RUO) version. 
 

Video Overview:

About us

How we pack

 VIEW SOME OF THE CUSTOMERS WE HELPED HERE 

CRITICAL TECHNICAL STEPS WE TAKE TO ENSURE INSTRUMENT PERFORMANCE & CUSTOMER SATISFACTION

  They want to ensure the cytometer undergoes full fluidics system decontamination, flushing, and inspection to protect the valves and flow sensors from damage.

  They want to ensure the cytometer has no residual sheath salt buildup in the flow cell, which could skew scatter data and, and if improperly cleaned, can damage the flow cell.

  They want to confirm that all detectors, including forward scatter (FSC) and side scatter (SSC) detectors, can be aligned and calibrated for particle sizing and granularity resolution.

  They want assurance that baseline performance has been verified using standardized particles, with data or log files available for review.

  They want to confirm that electronic noise and EMI levels at the detector/amplifier stage remain within tolerance and will not distort signal fidelity.

  They want to ensure that no fiber optic cables are missing, misrouted, or defective, which can lead to diminished fluorophore excitation or signal dropout.

  They want to confirm that no power degradation or misalignment in laser paths is detected through precise mW-level measurement at the final optics output.

  They want to ensure that optical filters and dichroic mirrors have been inspected for delamination, spectral shift, or contamination that could compromise signal specificity.

  They want to ensure the sample flow rate is linear and stable across a full range of flow settings, verified with volumetric calibration and pressure profiling.

  They want to ensure the system passes automated QC and performance tracking with reproducible CVs for bead standards across multiple channels.

  They want to ensure the fluidics pump assembly does not exhibit pulsation, drift, or backpressure due to check valve wear or micro-leaks.

  They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

*LA* Large Crate
525#

COMMON QUESTIONS AND ANSWERS BELOW