Laboratory

Robotic Arm(s): Flexible Channel Arm with (Span-8 FCA), Multi-Channel Arm (MCA), RGA Gripper Arm
Pipetting Channel(s): 8 (FCA) and 96 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Run time: 13–44 hr
Maximum output per run: 6 Tb
Maximum reads per run: 20B single reads; 40B paired-end reads
Maximum read length: 2 × 250 bp
Laser Wavelengths: 532 nm, 660 nm, 780 nm, 790 nm

Detector modules: Variable Wavelength, Refractive Index, MALS, ViscoStar Detector
Flow range: 0.2 to 10 mL/min
Injection range: 0.1-100 uL
Pump type: isocratic
System pressure operating range: up to 600 bar

Robotic Arm(s): Flexible Channel Arm with Air System (Span-8 Air FCA), Multi-Channel Arm (MCA), Robotic Gripper Arm (RGA)
Pipetting Channel(s): 8 (FCA), 96 (MCA) (up to 384 with accessory 384-head, sold separately)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Robotic Arm(s): Flexible Channel Arm with Air System (Span-8 Air FCA), Multi-Channel Arm (MCA), Robotic Gripper Arm (RGA)
Pipetting Channel(s): 8 (FCA), 96 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Camera: sCMOS
Throughput: >160K wells/day confocal, >200K wells/day widefield
4 position objective changer/ 5-position dichroic filter wheel/ 8-position emission filter wheel
Plates supported: up to 1536-well plates
96-well plate scan time, 2 colors, 1 site/well: 2 minutes

E-BD32 gassing lid for standard microfluidic experiments
Volume: 800 – 2000 uL
Temperature.: -8 DegC – 50 DegC
Shaking frequencies: 100 rpm – 1500 rpm
Filter configuration: 6 different filters

Detector modules: Variable Wavelength & Refractive Index Detectors
Flow range: 0.2 to 10 mL/min
Injection range: 0.1-100 uL
Pump type: isocratic
System pressure operating range: up to 600 bar

(5) Lasers: Red (640nm), YG (561nm), Blue (488nm), Violet (405nm), UV (355nm)
(18) Colors simultaneously; (20) Detector Channels
Fluorescence Sensitivity: FITC 80 (MESF-FITC), PE: 30 (MESF-PE)
Data Acquisition Rate: 40,000 events/sec
Sample Handling: 96 or 384 well microplates or manually loaded tubes

Plate format: 48-wells (32 reactor/16 reservoir wells possible)
Volume: 800 – 2400 uL
Temperature.: -8 DegC – 50 DegC
Shaking frequencies: 100 rpm – 1500 rpm
Filter configuration: 6 different filters

Plate format: 48-wells (32 reactor/16 reservoir wells possible)
Volume: 800 – 2400 uL
Temperature.: -8 DegC – 50 DegC
Shaking frequencies: 100 rpm – 1500 rpm
Filter configuration: 6 different filters

Imaging mode: Fluorescence, phosphor imaging, densitometry, and chemiluminescence (Dark scan)
Lasers: LD488, SHG532, LD635, LD685, LD785
Internal Filters: IP, Cy2, Cy3, Cy5, IRshort, IRlong
PMTs: Bi-alkali, multi-alkali
Measurable dynamic range: >5 orders of magnitude

Optics: (4) Lasers: Violet (405nm), Blue (488nm), Yellow/ Green (561nm), Red (650nm), (16) Colors simultaneously, (18) Detector Channels
Forward Scatter Detection: Photodiode detector with a 488/10 BP filter
Side Scatter Detector: Photomultiplier tube (PMT) with a 488/10 BP filter
Lasers: 405 violet laser detection channels: A (780/60, 750LP), B (710/50, 690LP), C (670/30, 635LP), D (610/20, 600LP), E (525/50, 505LP) F (450/50, GG420); 488 blue laser detection channels: A (710/50, 685LP), B (530/30, 505LP); 561 yelllow/green laser detection channels: A (780/40, 750LP), B (710/50, 685LP), C (670/30, 635LP), D (610/20, 600LP), E (586/15); 650 red laser detection channels: A (780/60, 750LP) B (730/45, 690LP), C (670/30, RG665)
Fluorescence Sensitivity: FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC), PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE), PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5), APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)

Number of solvents: four solvents; operating flow rate range: 0.010 to 4.000 mL/min, in 0.001-mL increments
Labware compatibility: (2x) 96- or 384-well plates, other options
Injection volume range: 0.1 to 50.0 uL, in 0.1-uL increments; 10-uL loop
Column compartment temperature range: 4 to 90 DegC settable in 0.1 DegC increments
PDA detector wavelength range: 190 to 800 nm; Mass range QDA Detector: 30 - 1250 m/z

(4) Laser: 405nm Violet, 488nm Blue, 561nm Yellow, 637nm Red; (14) Colors Simultaneously
Channel Filters: (Violet) 440/50, 512/25, 603/48, 710/50 (Blue) 530/30, 590/40, 695/40 (Yellow) 585/16, 620/15, 695/40, 780/60 (Red) 670/14, 720/30, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Data Acquisition Speed: up to 35,000 events/second with 34 parameters, based on a 10% coincidence rate per poisson statistics
Maximum Electronic Speed: 65,000 events/second with all parameters

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 6 Flr channels; 488nm blue laser: 2 Flr channels; 561nm yellow laser: 3 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

(4) Laser: 405nm Violet, 488nm Blue, 561nm Yellow, 637nm Red; (14) Colors Simultaneously
Channel Filters: (Violet) 440/50, 512/25, 603/48, 710/50; (Blue) 530/30, 590/40 695/40; (Yellow) 585/16, 620/15, 695/40, 780/60; (Red) 670/14, 720/30, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

(4) Laser: 405nm Violet, 488nm Blue, 561nm Yellow, 637nm Red; (14) Colors Simultaneously
Channel Filters: (Violet) 440/50, 512/25, 603/48, 710/50; (Blue) 530/30, 590/40 695/40; (Yellow) 585/16, 620/15, 695/40, 780/60; (Red) 670/14, 720/30, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Data Acquisition Speed: up to 35,000 events/second

Robotic Arm(s): Flexible Channel Arm with Liquid System (Span-8 Liquid FCA), Robotic Gripper Arm (RGA)
Channels: 8
Volume: up to 1250 ul
Tips Type: non disposable, fixed
Application: liquid handling and sample preparation

(3) Lasers: 405nm Violet / 488nm Blue / 640nm Red
(9) Colors simultaneously / (11) Detectors
4-way sorting: 1.5-, 2.0-, and 5.0-mL tubes; 2-way sorting: 1.5-, 2.0- and 5.0-mL tubes
Fluorescence sensitivity: FITC: <110 molecules (MESF-FITC); PE: <30 molecules (MESF-PE)
Acquisition rate: max 40,000 events per second, independent of the number of parameters

Robotic Arms: 96AM, Gripper upgrade
Channels: 96 (max 250uL per channel)
Usable Tips: 10, 30, 70, 200, 250uL
Microplates: 96-well formats
(9) Customizable Deck Positions

Microscope Orientation & Grade: Inverted, Superior Grade
Illumination: Brightfield, Fluorescence with DAPI,FITC,Texas Red (DIC, Polarization, PH or other filters can be added)
Objectives: 5x/0.15 Plan-Neofluar, 10x/0.30 Plan-Neofluar Ph1, 20x/0.40 Plan-Neofluar Ph2 (other objectives available)
Stage: Motorized XY Stage
Motorized Features: Lightpath, Objective Turret, Fluor Cube Turret, Z axis focus, XY Stage, Condenser (Shutter may be available)

Orientation: Inverted
Illumination Type: Brightfield w/ DIC, Fluorescence
Objectives: 4x/0.13 Plan Fluor, 10x/0.30 Plan Fluor DIC L/N1, 20x/0.75 Plan Apo VC
Light Source: PRIOR Brightfield LED, EXFO Metal Halide
Stage: Mechanical XY

Orientation: Inverted
Illumination Type: Brightfield w/ Fluorescence
Objectives: 4x/0.13 Plan Fluor WD 17.1, 10x/0.30 OFN25 Ph1 DL, 20x/0.75 Plan APO VC
Light Sources: 12V LED Lamp, EXFO X-Cite 120 Metal Halide
Stage: XY Motorized