Laboratory

5 lasers: 355, 405, 488, 561, 637 nm
~147 fluorescence channels + FSC/SSC
High sensitivity: PE ≤4 MESF, APC ≤7 MESF
Up to 40,000 events/sec, detection to ~160 nm
Autosampler: 96/384-well plates and 5 mL tubes, 4°C Peltier cooling

Orientation: Inverted
Illumination Type: Brightfield, Polarization, Fluorescence, and Confocal Fluorescence
Objectives: APO 10x/0,40 CS2, APO 20x/0,75 CS2, APO 40x/1,10 W motCORR CS2, APO 40x/1,30 Oil CS2, APO 63x/1,40 Oil CS2
Light Sources: LED brightfield lamp, LED3 Fluorescence source, Stellaris 5 (White Laser and 405nm Diode)
Stage: Motorized

(5) Lasers: Red 40mW(640nm), YG 50mW(561nm), Blue 50mW(488nm), Violet 50mW(405nm), UV (355nm)
Laser Color Allocation (Laser/Channel Count): Blue/2, Red/3, Violet/6, UV/2, Yellow/5
Fluorescence Sensitivity: FITC 80 (MESF-FITC), PE: 30 (MESF-PE)
Data Acquisition Rate: 40,000 events/sec
Sample Handling: 96 or 384 well microplates or manually loaded tubes

5 lasers, 30 colors, 32 detectors with solid‑state design
SiPM detection, FSC sensitivity 0.4 um, SSC 0.1 um
Fluorescence sensitivity: FITC ≤40 MESF, PE ≤10 MESF
100,000 events/sec with syringe‑pump volumetric counting
NovoSampler Q supports tubes and multi‑well plates

Robotic Arms: Span-8 w/ Gripper, 96-Multichannel Arm w/ Gripper
Channels: 8, 96
Volume: 1-1000 on Span-8, 5-1070 on 96-Multichannel
Disposable Tips: P20, P30, P30XL, P250, P1000 (P1000 for span 8 only)
Application: liquid handling and sample preparation

(5) Lasers: Blue 488 nm (200mW) / Red 640 nm (140mW) / Violet 405 nm (200mW) / UV 355 nm (109mW) / Yellow-Green 561 nm (150mW)
Laser Color Allocation (Laser/Channel Count): 6-Blue, 3-Red, 7-Violet, 5-Yellow/Green, 8-UV
Acquisition rate: 40,000 events/second with beads
Fluorescence resolution: (CV) PI: Area, <3.0%, full G^0 /G^1 peak for PI-stained CEN
Sample Handling: HTS High Throughput Sampler

5 lasers, 30 colors, 32 detectors with solid‑state design
SiPM detection, FSC sensitivity 0.4 um, SSC 0.1 um
Fluorescence sensitivity: FITC ≤40 MESF, PE ≤10 MESF
100,000 events/sec with syringe‑pump volumetric counting
NovoSampler Q supports tubes and multi‑well plates

Robotic Arm(s): Flexible Channel Arm with (Span-8 FCA), Multi-Channel Arm (MCA), RGA Gripper Arm
Pipetting Channel(s): 8 (FCA) and 96 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Robotic Arm(s): Flexible Channel Arm with Air System (Span-8 FCA), Multi-Channel Arm (MCA), Robotic Gripper Arm (RGA)
Pipetting Channel(s): 8 (FCA) and 96 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Optofluidic single-cell manipulation platform
OEP-based light-directed cell positioning
500 pL-1 nL NanoPen chamber volumes
Five filter cubes: 4 for fluorescence and 1 for brightfield/OEP
Per-chip temperature regulation (10–40DegC)

Optics: (4) Lasers: Violet (405nm), Blue (488nm), Yellow/ Green (561nm), Red (650nm)
Laser Color Allocation: Blue/2, Red/3, Violet/6, Yellow/5
Data Acquisition Rate: 40,000 events/sec
Fluorescence Sensitivity: FITC 80 (MESF-FITC), PE: 30 (MESF-PE), PE-Cy5: 10 (MESF-PE Cy5), APC: 70 (MESF-APC)
Sample Handling: 96 or 384 well microplates or manually loaded tubes

Robotic Arm(s): Flexible Channel Arm with (Span-8 FCA), Multi-Channel Arm (MCA), RGA Gripper Arm
Pipetting Channel(s): 8 (FCA) and 96/384 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Orientation: Inverted
Illumination Type: Brightfield, Fluorescence
Objectives: Nikon Plan Apo λ 10x/0.45, Nikon S Fluor 20x/0.75, Nikon Apo 60x/1.40 Oil λS
Light Sources: LED Brightfield lamp, Lumencor SOLA, LU-N3-SIM laser unit
Stage: Motorized XY

Capable Studies: Widefield, Brightfield, Confocal
Wavelength range: 7 class 3B lasers (405 nm/100 mW; 450 nm/75 mW; 488 nm/120 mW; 561 nm/100 mW; 594 nm/100 mW; 647 nm/120 mW; 785 nm/100 mW)
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanXApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Focus resolution: Sub-micron step size with advanced micro-stepping and double-stepping capabilities 

Optofluidic single-cell manipulation platform
OEP-based light-directed cell positioning
500 pL-1 nL NanoPen chamber volumes
Five filter cubes: 4 for fluorescence and 1 for brightfield/OEP
Per-chip temperature regulation (10–40DegC)

Membrane Width: 5 mm - 120 mm; speed adjustable up to 100 mm/sec
(7) Dispensers: (4) BioJet (solenoid valve) and (3) AirJet (pneumatic) dispensers with tandem pumps
Dual drying towers, each with 3 IR-heated zones and real-time temperature feedback
Keyence CA-H048MX (2448×2048 px) camera with CA-LH8 (8 mm, f/1.4) lens for in-line vision inspection
Capstan-driven tension control, diptank module with level sensing, and automated marking system

8 independently controlled single-use bioreactor stations
100–250 mL working volume per vessel
Agitation range 100–4500 rpm; 18–55°C ±0.5°C
pH 2.0–8.5 (±0.02); DO 0–200% air saturation
5 syringe pumps + 1 peristaltic pump per vessel

Pipetting System: 8×1 mL CORE II channels; CORE II 96 multi-probe head; 1–1000 uL range
Sample Loading: automatic sample loading; 1D autoload barcode scanner
Transport: Internal plate gripper (iSWAP); TrackGripper
Pipetting Control: CO‑RE; air displacement; LLD; pLLD; ADC; MAD; TADM
KingFisher Presto (96 samples/run); 5-115 DegC heating/cooling

4 Lasers/ 48 Fluorescence Channels/ 51 Detector
Violet (405 nm) Blue (488 nm) Red (640 nm) Yellow-Green (561 nm)
Sample input: manual (12x75 mm polystyrene and polypropylene tubes), plate loader (96-MP, 96-DW MP, 40-tube racks)
Sample flow rates:  Tube mode:15 - 60 uL/min Plate mode: 15 - 100 uL/min
Data acquisition rate: up to 35,000 events/s

Robotic Arms: Span-8 w/ Gripper, 96-Multichannel Arm w/ Gripper
Channels: 8, 96
Volume: 1-1000 on Span-8, 5-1070 on 96-Multichannel
Disposable Tips: P20, P30, P30XL, P250, P1000 (P1000 for span 8 only)
Application: liquid handling and sample preparation

Robotic Arms: Span-8 w/ Gripper, 384-Multichannel Arm w/ Gripper
Channels: 8, 384
Volume: 1-1000 on Span-8, 0.5-60 ul on 384-Multichannel
Disposable Tips: 25, 40ul (Filtered) 30, 50ul (Non-Filtered) disposable tips for 384-MCA; P20, P30, P30XL, P250, P1000 for span-8
Application: liquid handling and sample preparation

4 lasers, 25 colors, 27 detectors with solid‑state design
SiPM detection, FSC sensitivity 0.4 um, SSC 0.1 um
Fluorescence sensitivity: FITC ≤40 MESF, PE ≤10 MESF
100,000 events/sec with syringe‑pump volumetric counting
NovoSampler Q supports tubes and multi‑well plates

Plate format: 32-wells with E-BD32 Micro Fluidic Gassing Lid
Cultivation well filling volume: 800 – 2400 uL (rpm and microtiter plate type dependent)
Temperature: - 8 °C below AT - 50 °C
Shaking frequencies: 100 rpm-1500 rpm
Installed filters: biomass, 3x pH (HP8, LG1, ph51), 2x DO (Pst3, RF)

(5) Lasers: Blue 488 nm (200mW) / Red 628 nm (200mW) / Violet 405 nm (200mW) / UV 355 nm (60mW) / Yellow-Green 552 nm (200mW)
Laser Color Allocation (Laser/Channel Count): 6-Blue, 3-Red, 8-Violet, 5-Yellow/Green, 7-UV
Acquisition rate: 40,000 events/second with beads
Fluorescence resolution: (CV) PI: Area, <3.0%, full G^0 /G^1 peak for PI-stained CEN
Sample Handling: HTS High Throughput Sampler

(5) Lasers: Blue 488nm / Red 637nm / Violet 405nm/ UV 355nm/ Yellow Green 561nm
Laser Color Allocation: 4-Blue, 3-Red, 6-Violet, 4-Yellow/Green, 6-UV
Acquisition rate: 40,000 events/second with bead
Fluorescence resolution: (CV) PI: Area, <3.0%, full G^0 /G^1 peak for PI-stained CEN
Sample Handling: HTS High Throughput Sampler