Laboratory

5 lasers: 355, 405, 488, 561, 637 nm
~147 fluorescence channels + FSC/SSC
High sensitivity: PE ≤4 MESF, APC ≤7 MESF
Up to 40,000 events/sec, detection to ~160 nm
Autosampler: 96/384-well plates and 5 mL tubes, 4°C Peltier cooling

Orientation: Inverted
Illumination Type: Brightfield, Polarization, Fluorescence, and Confocal Fluorescence
Objectives: APO 10x/0,40 CS2, APO 20x/0,75 CS2, APO 40x/1,10 W motCORR CS2, APO 40x/1,30 Oil CS2, APO 63x/1,40 Oil CS2
Light Sources: LED brightfield lamp, LED3 Fluorescence source, Stellaris 5 (White Laser and 405nm Diode)
Stage: Motorized

Robotic Arms: Span-8 w/ Gripper, 96-Multichannel Arm w/ Gripper
Channels: 8, 96
Volume: 1-1000 on Span-8, 5-1070 on 96-Multichannel
Disposable Tips: P20, P30, P30XL, P250, P1000 (P1000 for span 8 only)
Application: liquid handling and sample preparation

(5) Lasers: Blue 488 nm (200mW) / Red 640 nm (140mW) / Violet 405 nm (200mW) / UV 355 nm (109mW) / Yellow-Green 561 nm (150mW)
Laser Color Allocation (Laser/Channel Count): 6-Blue, 3-Red, 7-Violet, 5-Yellow/Green, 8-UV
Acquisition rate: 40,000 events/second with beads
Fluorescence resolution: (CV) PI: Area, <3.0%, full G^0 /G^1 peak for PI-stained CEN
Sample Handling: HTS High Throughput Sampler

Detection technology: Bio-Layer Interferometry (BLI)
16 spectrometers with up to 16 simultaneous reads
Molecular weight: >150 Da
Sample volume: 40-220 uL (depending on plate type)
Microplate positions: 96- and 384-well microplates, two plate positions

Robotic Arm(s): Flexible Channel Arm with (Span-8 FCA), Multi-Channel Arm (MCA), RGA Gripper Arm
Pipetting Channel(s): 8 (FCA) and 96 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Robotic Arm(s): Flexible Channel Arm with Air System (Span-8 FCA), Multi-Channel Arm (MCA), Robotic Gripper Arm (RGA)
Pipetting Channel(s): 8 (FCA) and 96 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Optofluidic single-cell manipulation platform
OEP-based light-directed cell positioning
500 pL-1 nL NanoPen chamber volumes
Five filter cubes: 4 for fluorescence and 1 for brightfield/OEP
Per-chip temperature regulation (10–40DegC)

Robotic Arm(s): Flexible Channel Arm with (Span-8 FCA), Multi-Channel Arm (MCA), RGA Gripper Arm
Pipetting Channel(s): 8 (FCA) and 96/384 (MCA)
Pipetting Volume: 0.5 to 1000uL
Tips: Disposable Tips 10, 50, 200, 350, 1000uL can be mounted
Application: liquid handling and sample preparation

Orientation: Inverted
Illumination Type: Brightfield, Fluorescence
Objectives: Nikon Plan Apo λ 10x/0.45, Nikon S Fluor 20x/0.75, Nikon Apo 60x/1.40 Oil λS
Light Sources: LED Brightfield lamp, Lumencor SOLA, LU-N3-SIM laser unit
Stage: Motorized XY

Capable Studies: Widefield, Brightfield, Confocal
Wavelength range: 7 class 3B lasers (405 nm/100 mW; 450 nm/75 mW; 488 nm/120 mW; 561 nm/100 mW; 594 nm/100 mW; 647 nm/120 mW; 785 nm/100 mW)
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanXApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Focus resolution: Sub-micron step size with advanced micro-stepping and double-stepping capabilities 

Optofluidic single-cell manipulation platform
OEP-based light-directed cell positioning
500 pL-1 nL NanoPen chamber volumes
Five filter cubes: 4 for fluorescence and 1 for brightfield/OEP
Per-chip temperature regulation (10–40DegC)

Membrane Width: 5 mm - 120 mm; speed adjustable up to 100 mm/sec
(7) Dispensers: (4) BioJet (solenoid valve) and (3) AirJet (pneumatic) dispensers with tandem pumps
Dual drying towers, each with 3 IR-heated zones and real-time temperature feedback
Keyence CA-H048MX (2448×2048 px) camera with CA-LH8 (8 mm, f/1.4) lens for in-line vision inspection
Capstan-driven tension control, diptank module with level sensing, and automated marking system

8 independently controlled single-use bioreactor stations
100–250 mL working volume per vessel
Agitation range 100–4500 rpm; 18–55°C ±0.5°C
pH 2.0–8.5 (±0.02); DO 0–200% air saturation
5 syringe pumps + 1 peristaltic pump per vessel

Pipetting System: 8×1 mL CORE II channels; CORE II 96 multi-probe head; 1–1000 uL range
Sample Loading: automatic sample loading; 1D autoload barcode scanner
Transport: Internal plate gripper (iSWAP); TrackGripper
Pipetting Control: CO‑RE; air displacement; LLD; pLLD; ADC; MAD; TADM
KingFisher Presto (96 samples/run); 5-115 DegC heating/cooling

4 Lasers/ 48 Fluorescence Channels/ 51 Detector
Violet (405 nm) Blue (488 nm) Red (640 nm) Yellow-Green (561 nm)
Sample input: manual (12x75 mm polystyrene and polypropylene tubes), plate loader (96-MP, 96-DW MP, 40-tube racks)
Sample flow rates:  Tube mode:15 - 60 uL/min Plate mode: 15 - 100 uL/min
Data acquisition rate: up to 35,000 events/s

Robotic Arms: Span-8 w/ Gripper, 96-Multichannel Arm w/ Gripper
Channels: 8, 96
Volume: 1-1000 on Span-8, 5-1070 on 96-Multichannel
Disposable Tips: P20, P30, P30XL, P250, P1000 (P1000 for span 8 only)
Application: liquid handling and sample preparation

Robotic Arms: Span-8 w/ Gripper, 384-Multichannel Arm w/ Gripper
Channels: 8, 384
Volume: 1-1000 on Span-8, 0.5-60 ul on 384-Multichannel
Disposable Tips: 25, 40ul (Filtered) 30, 50ul (Non-Filtered) disposable tips for 384-MCA; P20, P30, P30XL, P250, P1000 for span-8
Application: liquid handling and sample preparation

Plate format: 32-wells with E-BD32 Micro Fluidic Gassing Lid
Cultivation well filling volume: 800 – 2400 uL (rpm and microtiter plate type dependent)
Temperature: - 8 °C below AT - 50 °C
Shaking frequencies: 100 rpm-1500 rpm
Installed filters: biomass, 3x pH (HP8, LG1, ph51), 2x DO (Pst3, RF)

(5) Lasers: Blue 488 nm (200mW) / Red 628 nm (200mW) / Violet 405 nm (200mW) / UV 355 nm (60mW) / Yellow-Green 552 nm (200mW)
Laser Color Allocation (Laser/Channel Count): 6-Blue, 3-Red, 8-Violet, 5-Yellow/Green, 7-UV
Acquisition rate: 40,000 events/second with beads
Fluorescence resolution: (CV) PI: Area, <3.0%, full G^0 /G^1 peak for PI-stained CEN
Sample Handling: HTS High Throughput Sampler

(5) Lasers: Blue 488nm / Red 637nm / Violet 405nm/ UV 355nm/ Yellow Green 561nm
Laser Color Allocation: 4-Blue, 3-Red, 6-Violet, 4-Yellow/Green, 6-UV
Acquisition rate: 40,000 events/second with bead
Fluorescence resolution: (CV) PI: Area, <3.0%, full G^0 /G^1 peak for PI-stained CEN
Sample Handling: HTS High Throughput Sampler

(5) Lasers: Blue 488nm / Red 637nm / Violet 405nm/ UV 355nm/ Yellow Green 561nm
Laser Color Allocation: 3-Blue, 3-Red, 6-Violet, 4-Yellow/Green, 6-UV
Acquisition rate: 40,000 events/second with bead
Fluorescence resolution: (CV) PI: Area, <3.0%, full G^0 /G^1 peak for PI-stained CEN
Sample Handling: HTS High Throughput Sampler

Excitation lasers: Violet (405 nm), Blue (488 nm), Yellow-green (561 nm), Far red (640 nm)
Laser/Channel Count: 7V-7B-7Y-4R; Scatter parameters: standard FSC and SSC 488 nm
Nozzle: 100 um ceramic nozzle, 30 PSI
Sample input tube types: 1.5 mL, 5 mL, and 15 mL
Temperature control: 4 DegC-37 DegC for input and output of all media types

Andor Zyla sCMOS camera with single-disk spinning confocal optics (60 µm pinhole)
Laser and image-based autofocus with encoded XYZ stages offering <25 nm positioning resolution
Automated objective changer with 4×, 10×, 20× water, and 60× water immersion objectives
Supports slides and 1–1536 well plates; throughput >160K wells/day confocal imaging
Environmental control and water immersion modules

Orientation: Inverted
Illumination Type: Brightfield with Fluorescence
Objectives: 5x/0.15 HC PL FLUOTAR, 10x/0.30 HC PL FLUOTAR, 20x/0.50 HC PL FLUOTAR , 40x/0.80 HC PL FLUOTAR
Light Sources: LED brightfield lamp, LED5 Fluorescence source
Stage: Motorized