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BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors

BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

$52,995.00
CLICKABLE TABS BELOW

For Sale BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (5)Lasers/(18)Colors/(20)Detectors

 

  • Fully Functional
  • Current model
  • Serviced & in Stock
  • Good cosmetic condition
  • Warranty

 

Here is what you will receive:

  • BD LSRFortessa X-20 5-Laser Flow Cytometer
  • BD High Throughput Sampler
  • FACS Fluid Supply Station
  • Sheath Level Sensor
  • Waste Bottle + Level Sensor
  • 2-gallon Stainless Steel Cleaning Solution Vessel
  • Computer running Diva software ver9.0 (w/ 2 monitors, keyboard, mouse)
  • De Novo FCS Express analysis software (1-Year Subscription) RUO version
  • Communication and power cables.

 

When you purchase a flow cytometer from Boston Industries, you also receive a 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

  • User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
  • Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
  • Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
  • High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
  • Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
  • With FCS Express 7, you will have the latest Research Use analysis software.

 

BD LSRFortessa X-20 Cell Analyzer Flow Cytometer Configuration:

  • (5) Lasers: Red 40mW(640nm), YG 50mW(561nm), Blue 50mW(488nm), Violet 50mW(405nm), UV (355nm)
  • (18) Colors simultaneously
  • (20) Detector Channels

         - Forward Scatter Detection: Photodiode detector with a 488/10 bandpass (BP) filter

         - Side Scatter Detector: Photomultiplier tube (PMT) with a 488/10 BP filter

         - Red (640nm) - A (780/60, 750LP), B (730/45, 690LP), C (670/30, 665NM)

         - YG (561nm) - A (780/60,750LP), B (710/50, 685LP), C (670/30,635LP), D (610/20,600LP), E (586/15,0-58)

         - Blue (488nm) - A (710/50, 685LP), B (530/30, 505LP)

         - Violet (405nm) - A (780/60, 750LP), B (710/50, 685LP), C (670/30, 635LP), D (610/20, 600LP), E (525/50, 505LP), F (450/50, GG420)

         - UV (355nm) - A (525/50, 505LP), B (379/28, L-37)

 

  • Fluorescence Sensitivity:

         - FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)

         - PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)

         - PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5)

         - APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)

 

  • Fluorescence Resolution: Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Fluorescence Linearity: Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
  • Data Acquisition Rate: 40,000 events/sec with beads

 

Customization/Options: N/A

 

Testing performed by our in-house service team:

The BD LSRFortessa X-20 Cell Analyzer Flow Cytometer passed all tests and was found to be fully functional:

  • We tested the connection to the Diva 9 software.
  • We ran the fluidics priming and sequences.
  • We ran several cleaning cycles (long clean, flow cell clean, etc.)
  • We tested the HTS functionality.
  • We performed the optical alignment.
  • We completed the homing, priming, and cleaning.
  • We tested the run with 8 Peak Beads.

 

(A standardized set of Rainbow Beads with known optical properties was run through the system to assess the sensitivity, resolution, and positional accuracy of the detectors.
Successful detection of these beads in the expected fluorescence channels and with the anticipated intensities confirmed that the detectors are operating within the required specifications,
ensuring reliable data acquisition and analysis.)

 

Instrument Overview:

  • The BD LSRFortessa X-20 Cell Analyzer offers high performance in flow cytometry.
  • It supports up to 20 parameters simultaneously.
  • The LSRFortessa X-20 is customizable with up to 5 lasers.
  • Its advanced optics ensure precise data collection.
  • User-friendly software simplifies operation and analysis.
  • The BD LSRFortessa X-20 Flow Cytometer is ideal for complex, multi-parameter flow cytometry assays.

 

Instrument Application:

  • Immunophenotyping: Analyzing cell surface and intracellular markers to identify and quantify different cell populations.
  • Cell Cycle Analysis: Measuring DNA content to determine the distribution of cells in different phases of the cell cycle.
  • Apoptosis Detection: Assessing cell death by evaluating markers such as Annexin V and propidium iodide.
  • Cytokine Production: Quantifying cytokines and other soluble factors using bead-based immunoassays.
  • Transfection Efficiency: Evaluating the success of genetic material introduction into cells.
  • Multiparametric Analysis: Conducting complex studies involving multiple parameters to understand cellular functions and interactions

 

The key features:

  • Customizable with up to 5 lasers.
  • Supports 20 simultaneous parameters.
  • High-performance optics for precise data.
  • User-friendly software interface.
  • Compact and modular design.
  • Advanced fluidics system.

 

 De Novo FCS Express Software Features

  • Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
  • Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
  • Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
  • Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
  • Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
  • Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
  • Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
  • Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

 

 Benefits of FCS Express Software

  • Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
  • User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
  • Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
  • Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
  • Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
  • Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
    Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
  • Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

 

Additional technical info:

 

Note: Subscription is for the Research Use Only (RUO) version. 

 

Video Overview:

 

 

About us

 

How we pack

 

Why should you buy from us?

 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping.
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Many upgrade and repair parts are in stock.
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 

  • It will work!
  • Warranty
  • An extended warranty may be available.
  • You get after-sales technical support.
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

*LO* Large Crate

750#

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What steps do you take to verify, service, and confirm the functionality of the BD LSRFortessa X-20 Cell Analyzer Flow Cytometer?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

  • We tested the connection to the Diva 9 software.
  • We ran the fluidics priming and sequences.
  • We ran several cleaning cycles (long clean, flow cell clean, etc.)
  • We tested the HTS functionality.
  • We performed the optical alignment.
  • We completed the homing, priming, and cleaning.
  • We tested the run with 8 Peak Beads.

 

What supplies do I need?

You will need the consumables starting out: (Here are some helpful links)

 

Can I view the BD LSRFortessa X-20 Cell Analyzer Flow Cytometer in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.

 

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warranted for both parts and labor.
  • The customer should retain all packing materials during the warranty period.

 

What should I watch out for when buying a BD LSRFortessa X-20 Cell Analyzer Flow Cytometer used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

 

  • We tested the connection to the Diva 9 software.
  • We ran the fluidics priming and sequences.
  • We ran several cleaning cycles (long clean, flow cell clean, etc.)
  • We tested the HTS functionality.
  • We performed the optical alignment.
  • We completed the homing, priming, and cleaning.
  • We tested the run with 8 Peak Beads.

 

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this BD LSRFortessa X-20 Cell Analyzer Flow Cytometer elsewhere, possibly for less.

 

Here are some of the reasons for our price.

 

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We must purchase any required or missing accessories to complete the instruments.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing and shipping sensitive instruments.
  • We guarantee that the instrument will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

 

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

How do you pack? Have you previously packed and shipped BD LSRFortessa X-20 Cell Analyzer Flow Cytometer? Do you ship Internationally?

Packing

  • Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

 

How much will I save buying it pre-owned, and is it worth it?

A lot, and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.

 

Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

 

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

 

We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend thousands of dollars and receive a damaged instrument.

Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing.

We generally ship parcels either via UPS or freight for all larger goods.

Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant).

On freight shipments, we use 2” 1.7 lb. density foam, which provides excellent cushioning and support for the instrument.

Instruments are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provides cushioning in all directions and holds instruments firmly in place.

Final step is to photograph everything inside the crate and then seal it with screws.

 

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies