Waters Acquity UPLC H-Class Liquid Chromatography System w/ QDa & TUV Detectors

  For Sale Waters Acquity UPLC H-Class Liquid Chromatography System with QDa & TUV Detectors 

Pre-owned instrument

• Fully Functional
• Current model
• Serviced & in Stock
• Good cosmetic condition
• Warranty 

Here is what you will receive:

• Waters Acquity UPLC H-Class Quaternary Solvent Manager (model #186015018)
• Waters Acquity UPLC H-Class Sample manager FTN with 50uL sample loop (model #186015017)
• Waters Acquity UPLC Column Manager (model #186015043)
• Waters Acquity TUV Detector w/UPLC analytical flow cell 500nL (model #186015031)
• Waters Acquity QDa Detector (model #186008511)
• Solvents Tray
• PC with Empower 3 software
• Inter-communication cables
• Standard power cords

Please Note: The computer and Empower 3 software came to us with this Waters system. If the user’s application requires additional licenses, the end user will need to purchase them from Waters.

Waters Acquity UPLC H-Class Liquid Chromatography System Configuration:

• Dwell volume: <400 uL (standard 100 uL mixer)
• Integrated leak management: leak sensors, as standard, and safe leak handling
• Quantum synchronization: injection synchronization between the pump and sample manager enhances retention time reproducibility
• Settable flow rate range: 0.001 to 2.200 mL/min in 0.001 mL increments
• Maximum operating pressure: 15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min
• pH range: 2 to 12
• Unattended operation: leak sensors and safe leak handling, full 96-hour diagnostic data display through console software
• Cycle time: <30 s inject-to-inject

Acquity UPLC H-Class Quaternary Solvent Manager:

• Number of solvents: blend up to four solvents in any combination
• Solvent degassing: integrated vacuum degassing, four chambers; one additional chamber for the SM-FTN purge solvent
• Solvent blending: automated, on-line pH, ionic strength, and organic modifier blending from pure solvents with Auto-Blend Plus Technology
• Gradient formation: low-pressure mixing, quaternary gradient
• Gradient profiles: 11 gradient curves [including linear, step (2), concave (4), and convex (4)]
• Primary check valve: intelligent Intake Valve (i² Valve)
• Flow accuracy: ±1.0% at 0.5 to 2.0 mL/min using 100% A (with i² Valve); back pressure 600 to 1000 psi with degassed H₂0
• Flow precision: 0.075% RSD or ±0.020 min SD, whichever is greater, based on six replicates (with i² Valve)

Acquity UPLC H-Class Sample Manager FTN:

• Injection volume range: 0.1 to 50 uL, up to 1,000.0 uL with optional extension loops
• Accuracy (aspiration): ±0.2 uL, measured by fluid weight removed from vial with 10.0 uL injections averaged over 20 injections using a standard 100 uL syringe.
• Precision: <1% RSD 0.2 to 1.9 uL; <0.5% RSD 2.0 to 10.0 uL
• Number of sample plate positions: Two
• Compatible labware: 96 and 384 microplates, 48 position 2.00-mL vial plates, 48 position 0.65-mL micro-centrifuge tube plates, 24 position 1.50-mL micro-centrifuge tube plates
• Maximum sample capacity: 768 in two 384-well plates or, 96 in 2-mL vial holders
• Sample compartment: 4 to 40 DegC, settable in 0.1 DegC increments
• Temperature accuracy: ±0.5 DegC at sensor
• Temperature stability: ±1.0 DegC at sensor
• Injection needle wash: integral, active, programmable
• Minimum sample required: 3 uL residual, using total recovery; 2 mL vials (zero offset)
• Sample carryover: <0.004% caffeine (UV); <0.005% sulfadimethoxine (MS)
• Advanced Sample Manager capabilities: auto-dilution, auto-addition, and load-ahead

Acquity UPLC Column Manager:

• Column capacity: 2 columns (max. length of 150 mm with filter or guard column) or 4 columns (max. length of 50 mm) with optional tubing kit, up to 4.6 mm (I.D.)
• Switching valves: 2 nine-port, eight-position valves; provides programmable, automatic, random access switching, waste and bypass positions for rapid solvent changeover
• Column compartment temperature range: 4.0 to 90.0 DegC, settable in 0.1 DegC increments; 2 independent heat/cool zones per module, up to six zones in a stacked configuration
• Column compartments temperature accuracy: ±0.5 DegC
• Column compartments temperature stability: ±0.3 DegC
• Solvent conditioning: active pre-heating as standard

Acquity UPLC TUV Detector: 

• Wavelength range: 190 to 700 nm
• Wavelength accuracy: ±1 nm
• Sampling rate: up to 80 points/s
• Light source: deuterium lamp
• Dispersion volume: ≤0.8 uL (Analytical flow cell);
• Flow cell design: light-guiding UPLC intelligent technology flow cell
• Path length: 10 mm (analytical flow cell)
• Flow cell volume: 500 nL (analytical flow cell)
• Pressure limit: 1000 psi (all flow cells)
• Optical bandwidth: 5 nm

Acquity UPLC QDa Detector:

• Acquisition modes: Full scan MS, Selected Ion Recording (SIR)
• RADAR: collect highly specific quantitative data for target compounds with the ability to visualize all other components
• Mass range: 30 to 1250 m/z
• Scan speed: automatically optimized for enhanced data quality for acquisition rates of up to e.g. 10Hz for m/z 100 to 1000 or 20 Hz for m/z 50 to 500
• Mass accuracy: mass accuracy is better than ±0.2 Da over the full mass range
• Mass stability: mass drift is less than 0.1 Da over a 24-hour period
• Linearity of response: the linearity of response relative to sample concentration for a specified compound is up to four orders of magnitude from the limit of detection
• Ion polarity switching time: 25 ms to switch between positive and negative ion modes
• SIR acquisition rate: automatically-optimized for enhanced data quality for acquisition rates of up to 100Hz
• Number of SIR channels: up to 1024 SIR channels (32 functions, 32 channels per function) can be monitored in a single acquisition
• Mass resolution: automated mass resolution control (0.7 Da) for constant data quality

Customization/Options:

• Other detectors and flow cell options might be available. Please inquire with our sales team. 

Testing performed by our in-house service team:

The Waters Acquity UPLC H-Class Liquid Chromatography System with QDa & TUV Detectors passed all tests and was found to be fully functional:

• We inspect the Waters Acquity UPLC H-Class Liquid Chromatography System for its physical condition.
• We check for seamless communication with the Empower software.

Quaternary solvent manager:

• We carefully evaluate the system's startup process for proper functionality.
• We thoroughly assess the stability and consistency of solvent delivery.
• We evaluate the system's performance in creating and running gradient tests.
• We perform a gradient proportioning valve test to confirm accurate gradient composition.

• We run a seal-washing pump test to assess the functionality of the seal-washing system.
• We test the leak sensor.
• We perform the degasser health check to ensure proper degassing of solvents.
• We test the pressure limiter to validate the pressure regulation capabilities.
• We verify the accuracy of flow rates.
• We run leak tests to check for leaks under high-pressure conditions.

Sample manager FTN:

• We power on and ensure the proper initialization of the sample manager.
• We carry out a sample cooler set temperature accuracy test to ensure precise temperature control.
• We do a syringe leak test to detect any leaks in the syringe system that could affect sample delivery.
• We perform the needle seal test.
• We test the leak sensor.
• We perform a reproducibility test to assess the reproducibility of sample handling and injection.

Column manager:

• We power on and ensure the proper initialization of the column manager.
• We perform a column oven temperature accuracy test to verify the accuracy of temperature control in the column oven.

TUV Detector:

• We power on and ensure the proper initialization of the TUV detector.
• We check the remaining lamp life to ensure optimal performance.
• We run tests to verify accurate and reliable detection capabilities.
• We verify the leak sensor.

QDa Detector:

• We check the detector initializes correctly, establishing its baseline functionality and readiness for operation.
• We run tests to verify accurate and reliable detection capabilities.
• We run calibration to verify the accuracy and reliability of the detector's measurements.
• We test the detector's ability to distinguish between closely spaced peaks, which is important for accurately identifying and quantifying compounds.
• We run resolution checks.

Instrument Overview:

• The Waters Acquity UPLC H-Class Liquid Chromatography System is a high-performance instrument for analytical separations and quantification.
• It is equipped with QDa and TUV detectors, offering sensitive detection and reliable UV-Vis measurements.
• The system is designed for efficiency, delivering high-resolution separations and short analysis times.
• It provides flexibility and versatility for a wide range of analytical challenges.
• The Waters Acquity UPLC H-Class is a dependable choice for laboratories requiring accurate chromatographic analysis.

Instrument Application: Waters Acquity UPLC H-Class Liquid Chromatography System with QDa & TUV Detectors 

The Waters Acquity UPLC H-Class Liquid Chromatography System is commonly used in various chromatographic analysis and detection applications. including:

• Pharmaceutical analysis: for drug discovery, development, and quality control.
• Environmental analysis: for monitoring contaminants in water, soil, and air samples.
• Food and beverage analysis: for detecting additives, pesticides, and contaminants in food products.
• Clinical research: for analyzing biomarkers, metabolites, and pharmaceutical compounds in biological samples.
• Chemical research: for characterizing organic compounds and investigating reaction kinetics.

The key features of Waters Acquity UPLC H-Class Liquid Chromatography System:

• High-performance analytical separations and quantification.
• Sensitive detection with QDa and TUV detectors.
• Efficient with high-resolution separations and short analysis times.
• Flexibility for various analytical challenges.

Additional technical info:

• Datasheet: Waters Acquity UPLC H-Class Liquid Chromatography System datasheet
• Brochure: Waters Acquity UPLC H-Class Liquid Chromatography System brochure
• Manual: Waters Acquity UPLC H-Class Liquid Chromatography System manual
• Manual: Waters Acquity Quaternary Solvent Manager manual
• Manual: Waters Acquity Sample Manger FTN manual
• Brochure: Waters Acquity UPLC Detectors brochure
• Datasheet: Waters Acquity TUV Detector datasheet
• Manual: Waters Acquity TUV Detector manual
• Datasheet: Waters Acquity QDa Detector datasheet
• Manual: Waters Acquity QDa Detector manual

Video Overview:

About us

How we pack

Why should you buy from us?

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• It will work!
• Warranty
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.

COMMON QUESTIONS AND ANSWERS BELOW