PCR

Robotic Arm(s): Span-8, Gripper
Channels: 8
Volume: 1 - 1000ul
Disposable Tips: 200 or 1000ul
Application: liquid handling and sample preparation

Run Time: ~35 min for 384-well, ~30 min for 0.2 mL 96-well and 0.1 mL Fast 96-well (using Applied Biosystems Fast Master Mix)
Excitation/Detection Wavelengths: 6 excitation (450–670 nm), 6 emission (500–720 nm)
Temperature Uniformity: +/-0.5C, within 30 sec of reaching temperature set point
Supported Applications: Standard curve, Comparative Ct , Genotyping, Presence/Absence, HRM, Melt curve

Thermal Control: peltier-based, 4 - 99C; Heating Ramp Rate: up to 5.5 C/sec: Cooling Ramp Rate: up to 5.5 C/sec
Detection: CCD camera, 9M pixel, 12 x 12um pixel size
Fluorescence Excitation: 475nm, 530nm, 575nm; Fluorescence Emission: 525nm, 570nm, 630nm
Supported IFCs: Gene expression - 48.48 Dynamic Array IFC, 96.96 Dynamic Array IFC, 192.24 Gene Expression IFC, Flex Six Gene Expression IFC; Genotyping - 48.48 Dynamic Array IFC, 96.96 Dynamic Array IFC, 192.24 Genotyping IFC, Flex Six Genotyping IFC; Digital PCR - qdPCR 37K IFC, 48.770 Digital Array IFC, 12.765 Digital Array IFC
Software: Data Collection Biomark HD software, Real-Time PCR Analysis software, SNP Genotyping Analysis software, Digital PCR Analysis software, Melt Curve Analysis software

Thermal Control: peltier-based, 4 - 99C; Heating Ramp Rate: up to 5.5 C/sec: Cooling Ramp Rate: up to 5.5 C/sec
Detection: CCD camera, 9M pixel, 12 x 12um pixel size
Fluorescence Excitation: 475nm, 530nm, 575nm; Fluorescence Emission: 525nm, 570nm, 630nm
Supported IFCs: Gene expression - 48.48 Dynamic Array IFC, 96.96 Dynamic Array IFC, 192.24 Gene Expression IFC, Flex Six Gene Ex
Software: Data Collection Biomark HD software, Real-Time PCR Analysis software, SNP Genotyping Analysis software, Digital PCR An

Capacity: 96-Well Plate with installed 96-silber block
Temp Range: 37 - 95C
Accuracy: +/-0.2C of target temperature
96-well block: 4.8C/s
Dimensions (WxDxH): 57.4 x 58.8 x 49.7cm

Capacity: 96-Well Plate with installed 96-S block
Temp Range: 37 - 95C
Accuracy: +/-0.2C of target temperature
96-well block: 4.8C/s
Dimensions (WxDxH): 57.4 x 58.8 x 49.7cm

96-S Thermal Block w/ 96 well cycler cover
Excitation: 465nm, 498nm, 540nm, 610nm, 680 nm
Detection: 510nm, 580nm, 610nm, 645nm, 670nm, 700nm
Reaction Volume Range: 5-20ul
Reproducibility: CV =< 0.15%

Capacity: 96-Well Plate with installed 96-S block
Temp Range: 37 - 95C
Accuracy: +/-0.2C of target temperature
96-well block: 4.8C/s
Dimensions (WxDxH): 57.4 x 58.8 x 49.7cm

Capacity: 96-Well Plate with installed 96-S block
Temp Range: 37 - 95C
Accuracy: +/-0.2C of target temperature
96-well block: 4.8C/s
Dimensions (WxDxH): 57.4 x 58.8 x 49.7cm

Capacity: 96-Well Plate with installed 96-S block
Temp Range: 37 - 95C
Accuracy: +/-0.2C of target temperature
96-well block: 4.8C/s
Dimensions (WxDxH): 57.4 x 58.8 x 49.7cm

96-S Thermal Block w/ 96 well cycler cover
Excitation: 465nm, 498nm, 540nm, 610nm, 680 nm
Detection: 510nm, 580nm, 610nm, 645nm, 670nm, 700nm
Reaction Volume Range: 5-20ul
Reproducibility: CV =< 0.15%

Labware: QuantStudio 3D Digital PCR 20K Chip v2

Channels: 8 pipetting channels (20 - 200ul)
Precision: 20 - 200ul; less than 4% coefficient of variation
Capacity: up to 96 samples in increments of 8 samples
Dimensions (WxHxD): QIAcube HT 540 x 450 x 630mm (21 x 18 x 25in), VS04 Vacuum Station 495 x 520 x 400mm (19 x 20 x 16in)
Weight: QIAcube HT instrument 44kg (97lb), VS04 Vacuum Station 19kg (42lb)

Channels: 8 pipetting channels (20 - 200ul)
Precision: 20 - 200ul; less than 4% coefficient of variation
Capacity: up to 96 samples in increments of 8 samples
Dimensions (WxHxD): QIAcube HT 540 x 450 x 630mm (21 x 18 x 25in), VS04 Vacuum Station 495 x 520 x 400mm (19 x 20 x 16in)
Weight: QIAcube HT instrument 44kg (97lb), VS04 Vacuum Station 19kg (42lb)

Sampling Format: 3 x 16 well plates
Measurement Applications: Adhesion, Apoptosis, Cell Characterization, Cytotoxicity, Immune Cell Killing, Proliferation, Receptor Signaling, Virus Cytopathic Effects
Test Signal: 22 mV rms +/-20% with max. 5 mV DC offset at 10, 25 and 50 kHz
Environment: Temperature, +15C to +40C; relative humidity, 98% maximum without condensation
Resistor Values: 37.4, 64.9, 91.0, 115.0 Om, +/-0.5%

Sampling Format: 3 x 16 well plates
Measurement Applications: Adhesion, Apoptosis, Cell Characterization, Cytotoxicity, Immune Cell Killing, Proliferation, Receptor Signaling, Virus Cytopathic Effects
Test Signal: 22 mV rms +/-20% with max. 5 mV DC offset at 10, 25 and 50 kHz
Environment: Temperature, +15C to +40C; relative humidity, 98% maximum without condensation
Resistor Values: 37.4, 64.9, 91.0, 115.0 Om, +/-0.5%

Sampling Format: 3 x 16 well plates
Measurement Applications: Adhesion, Apoptosis, Cell Characterization, Cytotoxicity, Immune Cell Killing, Proliferation, Receptor Signaling, Virus Cytopathic Effects
Test Signal: 22 mV rms +/-20% with max. 5 mV DC offset at 10, 25 and 50 kHz
Environment: Temperature, +15C to +40C; relative humidity, 98% maximum without condensation
Resistor Values: 37.4, 64.9, 91.0, 115.0 Om, +/-0.5%

SNP Genotyping 2,304/9,216; Digital PCR 9,180
Excitation filters: 485-20, 530-20, 580-25 (center-width, in nm)
Emission filters: 525-25, 570-30, 630-30 (center-width, in nm)
Illumination: 175Watt Xenon arc lamp
Software: EP1 Data Collection software, Fluidigm SNP Genotyping Analysis software, Digital PCR Analysis software

Run Time: < 2 hours
Thermal Cycling System: peltier-based, 96-well block
Optical System: CCD camera witht halogen lamp excitation
Reaction Volumes: 20 - 100uL
Temperature Range: 4 - 100C

Run Time: <2h
Temperature Range: 4 - 100C
Detector: CCD camera with halogen lamp excitation; five-excitation and five-emission filters
Reaction Volume Range: 20-100uL

Chip supported: 151
Experiment tracking: Barcode
Gas pressure: Internal compressor
Interface: USB and Ethernet