ABI

Run Time: ~35 min for 384-well, ~30 min for 0.2 mL 96-well and 0.1 mL Fast 96-well (using Applied Biosystems Fast Master Mix)
Excitation/Detection Wavelengths: 6 excitation (450–670 nm), 6 emission (500–720 nm)
Temperature Uniformity: +/-0.5C, within 30 sec of reaching temperature set point
Supported Applications: Standard curve, Comparative Ct , Genotyping, Presence/Absence, HRM, Melt curve

Run Time: <2 hrs/run (Standard Mode), <40 min⁄run (Fast Mode)
Temperature Range (Metric): 4-100C
Detection Method: SYBR, Primer-Probe Detection
Reaction Volume Range: 10-30 ul (Standard curve experiments: 40 ul in standard mode is validated)
Optics: 4 emission filters, Blue LED excitation source, photodiode

Block Format: Interchangeable
Format: Fast 96-well plate
Temperature Range: 4-100C
Temperature Uniformity: +/-0.5C
Dimensions: 53.3 cm (21 in) (W) x 63.5 cm (25 in) (D) x 64.5 cm (25.4 in) (H)

Excitation/Detection Wavelengths: 6 excitation (450–670 nm), 6 emission (500–720 nm)
Format: Array Card
Temperature Range: 4-100C
Temperature Uniformity: +/-0.5C
Dimensions: 53.3 cm (21 in) (W) x 63.5 cm (25 in) (D) x 64.5 cm (25.4 in) (H)

Sample Capacity (block format): 96-well 0.2 mL block, 96-well 0.1 mL block (Fast), 384-well block, Applied Biosystems TaqMan Array Cards
Reaction Volume: 10-30uL for 96-well 0.1 mL block; 10-100uL for 96-well 0.2 mL block; 5-20uL for 384-well block; ~1.5uL for TaqMan Array Cards
Maximum Ramp Rate 96-well (0.2 mL): 6.5C/sec; 96-well (0.1 mL): 9.0C/sec; 384-well: 6.0C/sec; TaqMan Array Cards: 5.0C/sec
Average Sample Ramp Rate 96-well (0.2 mL): 3.66C/sec; 96-well (0.1 mL): 4.81C/sec; 384-well: 2.92C/sec; TaqMan Array Cards: 3.00C/sec
Temperature Uniformity: 0.4C; Temperature Range 4-99.9C

Block Format: Interchangeable
Format: 384-well plate
Temperature Range: 4-100C; Temperature Uniformity: +/-0.5C
Dimensions (WxDxH): 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in)
Excitation Filter: 470 +/- 15 nm, 520 +/- 10 nm, 550 +/- 10 nm, 580 +/- 10 nm, 640 +/- 10 nm, 662 +/- 10 nm; Emission Filter: 520 +/- 15 nm, 558 +/- 12 nm, 586 +/- 10 nm, 623 +/- 14 nm, 682 +/- 14 nm, 711 +/- 12 nm

Block Format: Interchangeable
Format: 384-well plate
Temperature Range: 4-100C; Temperature Uniformity: +/-0.5C
Dimensions (WxDxH): 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in)
Excitation Filter: 470 +/- 15 nm, 520 +/- 10 nm, 550 +/- 10 nm, 580 +/- 10 nm, 640 +/- 10 nm, 662 +/- 10 nm; Emission Filter: 520 +/- 15 nm, 558 +/- 12 nm, 586 +/- 10 nm, 623 +/- 14 nm, 682 +/- 14 nm, 711 +/- 12 nm

Block Format: Interchangeable
Format: 384-well plate
Temperature Range: 4-100C
Max Ramp rate: 3.5°C/Sec
Multiplexing: Yes

Block Format: Interchangeable
Format: 384-well plate
Temperature Range: 4-100C
Max Ramp rate: 3.5°C/Sec
Multiplexing: Yes

Block Format: Interchangeable
Format: 384-well plate
Temperature Range: 4-100C; Temperature Uniformity: +/-0.5C
Dimensions (WxDxH): 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in)
Excitation Filter: 470 +/- 15 nm, 520 +/- 10 nm, 550 +/- 10 nm, 580 +/- 10 nm, 640 +/- 10 nm, 662 +/- 10 nm; Emission Filter: 520 +/- 15 nm, 558 +/- 12 nm, 586 +/- 10 nm, 623 +/- 14 nm, 682 +/- 14 nm, 711 +/- 12 nm

Block Format: Interchangeable
Format: 96-well plate
Temperature Range: 4-100C; Temperature Uniformity: +/-0.5C
(5) coupled excitation and emission filter channels; (5) color detection
Dimensions (WxDxH): 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in)

Block Format: Interchangeable
Format: 96-well plate
Temperature Range: 4-100C; Temperature Uniformity: +/-0.5C
(5) coupled excitation and emission filter channels; (5) color detection
Dimensions (WxDxH): 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in)

Block Format: Interchangeable
Format: 96-well plate
Temperature Range: 4-100C; Temperature Uniformity: +/-0.5C
(5) coupled excitation and emission filter channels; (5) color detection
Dimensions (WxDxH): 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in)

Block Format: Interchangeable
Format: 96-well plate
Temperature Range: 4-100C; Temperature Uniformity: +/-0.5C
(5) coupled excitation and emission filter channels; (5) color detection
Dimensions (WxDxH): 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in)

Block Type: NON-Interchangeable
Format: 384-well plate
Detection Range 384-well: 450-650 nm/500-700 nm
Temperature Range: 4-100C
Temperature Uniformity: +/-0.4C

Block Type: NON-Interchangeable
Format: 384-well plate
Detection Range 384-well: 450-650 nm/500-700 nm
Temperature Range: 4-100C
Temperature Uniformity: +/-0.4C

(2) Laser: 488nm Blue, 637nm Red
(9) Detection Channels
Channel Filters: (Blue) 530/30, 574/26, 695/40, 780/60 (Red) 670/14, 720/30, 780/60
Sample Input Flow Rate: 12.5uL - 1,000uL/min
Fluid Consumption: ~1.8L/day

(4) Laser: 405nm Violet, 488nm Blue, 561nm Yellow, 637nm Red
(14) Colors Simultaneously
Emission Filters: (Violet) 440/50, 512/25, 603/48, 710/50 (Blue) 530/30, 590/40, 695/40 (Yellow) 585/16, 620/15, 695/40, 780/60 (Red) 670/14, 720/30, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Data Acquisition Speed: up to 35,000 events/second with 34 parameters, based on a 10% coincidence rate per poisson statistics

(3) Laser: 405nm Violet, 488nm Blue, 637nm Red
(11) Colors Simultaneously
Channel Filters (Violet): 440/50, 512/25, 603/48, 710/50
Channel Filters (Blue): 488/10+OD2(SSC), 530+30, 574/26, 695/40, 780/60
Channel Filters (Red): 670/14, 720/30, 780/60

Reaction Volume Range: 5 - 30uL
Sample Format: 96-Well Plates, optimized for 10uL reactions
Temperature Range: 4 - 100C
Sample ramp rate Fast mode: 3.5°C/sec
Multiplex Capability: Multiplex up to five targets per well

Reaction Volume Range: 5 - 30uL
Sample Format: 96-Well Plates, optimized for 10uL reactions
Temperature Range: 4 - 100C
Sample ramp rate Fast mode: 3.5°C/sec
Multiplex Capability: Multiplex up to five targets per well

96-plate Run Time: <30min
Temperature Range: 4-100C
Detectors: CCD camera with halogen lamp excitation
Reaction Volumes: 5-30uL
Sensitivity: Detection of 1 copy of template in a 20uL reaction for a single reporter TaqMan assay with 99.7% confidence