Thermo Fisher Scientific

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Processing time: 70–100 minutes; >85% isolation efficiency
Purity up to 95% with CTS Dynabeads CD3/CD28; viability unaffected
Isolation scale up to 1,000 mL (~10B T-cell input)
Pump speed range: 10–150 mL/min
Rocker angle: –30° to 30°; speed: 1–30 cpm

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Reaction Volume: 5–20 uL for 384-well block
Maximum Ramp Rate: 6.0DegC/sec
Average Sample Ramp Rate: 2.92DegC/sec
Temperature uniformity: 0.4DegC and range: 4-99.9DegC.
Filter or color combinations: 21

2L/9C/11D optical configuration, multicolor detection
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um
CytKick: <45 min/96‑well; <0.5% carryover

(3) Lasers: 405nm Violet, 488nm Blue, 561nm Yellow; (11) Colors Simultaneously
Channel Filters: (Violet) 440/50, 512/25, 603/48, 710/50; (Blue) 530/30, 590/40, 695/40; (Yellow) 585/16, 620/15, 695/40, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

(3) Lasers: 405nm Violet, 488nm Blue, 637nm Red; (11) Colors Simultaneously
Channel Filters: (Violet) 440/50, 512/25, 603/48, 710/50; (Blue) 530/30, 574/26, 695/40, 780/60; (Red) 670/14, 720/30, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Processing time: 70–100 minutes; >85% isolation efficiency
Purity up to 95% with CTS Dynabeads CD3/CD28; viability unaffected
Isolation scale up to 1,000 mL (~10B T-cell input)
Pump speed range: 10–150 mL/min
Rocker angle: –30° to 30°; speed: 1–30 cpm

4-plex digital PCR with 5 optical channels
20,480 microchambers per reaction
Dynamic range: 5 logs; ±10% precision
Up to 16 samples per run (MAP16 plate)
Sensitivity: 1 to 100,000 copies

Reaction Volume: 10-100uL for 96-well 0.2 mL block
Maximum Ramp Rate 96-well (0.2 mL): 6.5C/sec
Average Sample Ramp Rate 96-well (0.2 mL): 3.66C/sec
Temperature Uniformity: 0.4C; Temperature Range 4-99.9C
VeriFlex Blocks 3 or 6 independent temperature zones (96-well blocks)

Block Format: Interchangeable, 96-well 0.1 mL block (Fast)
Reaction Volume: 10–30 uL for 96-well 0.1 mL block
Maximum Ramp Rate: 9.0DegC/sec
Average Sample Ramp Rate: 4.81DegC/sec
Temperature Details: uniformity: 0.4DegC; range: 4-99.9DegC

Reaction Volume: 5–20 uL for 384-well block
Maximum Ramp Rate: 6.0DegC/sec
Average Sample Ramp Rate: 2.92DegC/sec
Temperature uniformity: 0.4DegC and range: 4-99.9DegC.
Filter or color combinations: 21

Block Format: 96-well block (0.2mL)
96 well reaction volume: 10-100 uL
Max. ramp rate: 3.66DegC/sec
Temperature Range: 4-99.9DegC
(5) Color detection, Excitation/emission range 450–600 nm/500–640 nm

Block Format: Fast 96-well block (0.1mL)
Reaction Volume: 10–30 uL
Maximum Ramp Rate: 9.0DegC/sec, Average Ramp Rate: 4.81DegC/sec
Temperature: uniformity at 0.4DegC and a range of 4-99.9DegC.
Filter combinations: 5; Excitation/emission range: 450–600 nm/500–640 nm

Block Format: 384-well block
Reaction Volume: 5–20 uL
Maximum Ramp Rate: 6.0DegC/sec; Average Ramp Rate: 2.92DegC/sec;
Temperature: uniformity at 0.4DegC and a range of 4-99.9DegC.
Filter combinations: 5; Excitation/emission range: 450–600 nm/500–640 nm

Centrifugal force: 3000 x g
Centrifuge chamber volume: 10 mL
Recommended input volume range: 50 mL-20 L
Peristaltic pump flow rate: 5-110 mL/min; 30-160 mL/min (kit dependent)
Cell count per chamber load: 5x10^7-5x10^9 cells

Centrifugal force: 3000 x g
Centrifuge chamber volume: 10 mL
Recommended input volume range: 50 mL-20 L
Peristaltic pump flow rate: 5-110 mL/min; 30-160 mL/min (kit dependent)
Cell count per chamber load: 5x10^7-5x10^9 cells

Centrifugal force: 3000 x g
Centrifuge chamber volume: 10 mL
Recommended input volume range: 50 mL-20 L
Peristaltic pump flow rate: 5-110 mL/min; 30-160 mL/min (kit dependent)
Cell count per chamber load: 5x10^7-5x10^9 cells

Recommended cell concentration: 20 × 10^6 to 100 × 10^6 cells/mL.
Electroporation chamber volume: 1 mL with 1–25 mL processing range.
Pulse voltage range: 500 to 2,500 V with 1–30 ms pulse width control.
Number of pulses: 1–10 with 500–1,000 ms pulse interval.
Run time: 7–22 min for 5–25 mL electroporation volumes.

Illumination: transmitted and RGB transmitted modes
Objective capacity: 4-position manual turret with 4x, 10x, 20x phase contrast objectives
Condenser: 4-position turret with clear aperture and 3-phase annuli
Camera: 3.2 MP CMOS (2064 × 1536), 3.45 µm pixel resolution
Fluorescence: 2-position chamber

Block format: 96-well isothermal block
PCR volume range: 10−100 uL
Thermal range: 0-105 DegC
Thermal accuracy: ±0.25 DegC (35 DegC to 99.9 DegC)
Max. ramp rate: 3.5 DegC/sec. (block), 1.8 DegC/sec. (sample)

Block format: 384-well isothermal block
PCR volume range: 5-10 uL
Thermal range: 0-105 DegC
Thermal accuracy: ±0.25 DegC (35 DegC to 99.9 DegC)
Max. ramp rate: 2.8 DegC/sec. (block), 1.6 DegC/sec. (sample)

(3) Laser: 405nm Violet, 488nm Blue, 637nm Red; (11) Colors Simultaneously
405nm V laser: 440/50, 512/25, 603/48, 710/50; 488nm B laser: 530/30, 574/26, 695/40, 780/60; 637nm R laser: 670/14, 720/30, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
CytKick Max Autosampler, External Fluid Supply