Thermo Fisher Scientific

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 6 Flr channels; 488nm blue laser: 2 Flr channels; 561nm yellow laser: 3 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Optics: 3 lasers, 10 colors, 12 detectors (Blue/3, Yellow/4, Red/3)
Acquisition: up to 35,000 events/sec; electronic speed up to 65,000 events/sec
Sample Analysis Volume: 20 uL to 4 mL; Custom sample flow rates 12.5–1,000 uL/min
Sensitivity: ≤80 MESF FITC; ≤30 MESF PE; ≤70 MESF APC
Autosampler: 96/384-well plates and microcentrifuge tubes; <45 min per 96-well plate

Processing time: 70–100 minutes; >85% isolation efficiency
Purity up to 95% with CTS Dynabeads CD3/CD28; viability unaffected
Isolation scale up to 1,000 mL (~10B T-cell input)
Pump speed range: 10–150 mL/min
Rocker angle: –30° to 30°; speed: 1–30 cpm

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Optics:(4) Lasers, (14) Colors, (16) Detectors
405nm violet laser: 4 Flr channels; 488nm blue laser: 3 Flr channels; 561nm yellow laser: 4 Flr channels; 637nm red laser: 3 Flr channels;
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

4-plex digital PCR with 5 optical channels
20,480 microchambers per reaction
Dynamic range: 5 logs; ±10% precision
Up to 16 samples per run (MAP16 plate)
Sensitivity: 1 to 100,000 copies

Block Format: Interchangeable, 96-well 0.1 mL block (Fast)
Reaction Volume: 10–30 uL for 96-well 0.1 mL block
Maximum Ramp Rate: 9.0DegC/sec
Average Sample Ramp Rate: 4.81DegC/sec
Temperature Details: uniformity: 0.4DegC; range: 4-99.9DegC

(3) Lasers: 405nm Violet, 488nm Blue, 561nm Yellow; (11) Colors Simultaneously
Channel Filters: (Violet) 440/50, 512/25, 603/48, 710/50; (Blue) 530/30, 590/40, 695/40; (Yellow) 585/16, 620/15, 695/40, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um

Illumination: 5-position chamber for 4 fluorescence light cubes plus brightfield imaging
Filter cubes (motorized switching): DAPI (357/447 nm), GFP (482/524 nm), RFP (542/593 nm), Cy5 (635/692 nm)
5-position automated turret: PlanApo N 2x/0.80, UPlanSApo 4x/0.16, UPlanSApo 10x/0.40, UCPlanFL N 20x/0.70, LUCplanFL N 40x/0.60
Stage: Motorized encoded X/Y scanning stage
Sample Compatibility: Slides, Well plates, Petri Dishes with included sample holders

Processing time: 70–100 minutes; >85% isolation efficiency
Purity up to 95% with CTS Dynabeads CD3/CD28; viability unaffected
Isolation scale up to 1,000 mL (~10B T-cell input)
Pump speed range: 10–150 mL/min
Rocker angle: –30° to 30°; speed: 1–30 cpm

Reaction Volume: 10-100uL for 96-well 0.2 mL block
Maximum Ramp Rate 96-well (0.2 mL): 6.5C/sec
Average Sample Ramp Rate 96-well (0.2 mL): 3.66C/sec
Temperature Uniformity: 0.4C; Temperature Range 4-99.9C
VeriFlex Blocks 3 or 6 independent temperature zones (96-well blocks)

Block Format: Interchangeable, 96-well 0.1 mL block (Fast)
Reaction Volume: 10–30 uL for 96-well 0.1 mL block
Maximum Ramp Rate: 9.0DegC/sec
Average Sample Ramp Rate: 4.81DegC/sec
Temperature Details: uniformity: 0.4DegC; range: 4-99.9DegC

Reaction Volume: 5–20 uL for 384-well block
Maximum Ramp Rate: 6.0DegC/sec
Average Sample Ramp Rate: 2.92DegC/sec
Temperature uniformity: 0.4DegC and range: 4-99.9DegC.
Filter or color combinations: 21

Block Format: 96-well block (0.2mL)
96 well reaction volume: 10-100 uL
Max. ramp rate: 3.66DegC/sec
Temperature Range: 4-99.9DegC
(5) Color detection, Excitation/emission range 450–600 nm/500–640 nm

Block Format: Fast 96-well block (0.1mL)
Reaction Volume: 10–30 uL
Maximum Ramp Rate: 9.0DegC/sec, Average Ramp Rate: 4.81DegC/sec
Temperature: uniformity at 0.4DegC and a range of 4-99.9DegC.
Filter combinations: 5; Excitation/emission range: 450–600 nm/500–640 nm

Block Format: 384-well block
Reaction Volume: 5–20 uL
Maximum Ramp Rate: 6.0DegC/sec; Average Ramp Rate: 2.92DegC/sec;
Temperature: uniformity at 0.4DegC and a range of 4-99.9DegC.
Filter combinations: 5; Excitation/emission range: 450–600 nm/500–640 nm

Centrifugal force: 3000 x g
Centrifuge chamber volume: 10 mL
Recommended input volume range: 50 mL-20 L
Peristaltic pump flow rate: 5-110 mL/min; 30-160 mL/min (kit dependent)
Cell count per chamber load: 5x10^7-5x10^9 cells

Centrifugal force: 3000 x g
Centrifuge chamber volume: 10 mL
Recommended input volume range: 50 mL-20 L
Peristaltic pump flow rate: 5-110 mL/min; 30-160 mL/min (kit dependent)
Cell count per chamber load: 5x10^7-5x10^9 cells

Centrifugal force: 3000 x g
Centrifuge chamber volume: 10 mL
Recommended input volume range: 50 mL-20 L
Peristaltic pump flow rate: 5-110 mL/min; 30-160 mL/min (kit dependent)
Cell count per chamber load: 5x10^7-5x10^9 cells

5-position manual objective turret with 4x, 10x, and 20x lenses.
Phase contrast ring with brightfield and multiple phase options.
Filter cubes for DAPI, GFP, and RFP channels.
Supports transmitted and RGB transmitted light illumination.
Mechanical X/Y stage for precise sample positioning.

5-position manual objective turret with 4x, 10x, and 20x lenses.
Phase contrast ring with brightfield and multiple phase options.
Filter cubes for DAPI, GFP, and RFP channels.
Supports transmitted and RGB transmitted light illumination.
Mechanical X/Y stage for precise sample positioning.

Sealing Plate Height: 8-46 mm
Foil Length per plate: 119-128 mm
Sealing Temperature Range: ambient to 199 DegC
Sealing Time: 0.5-9.9 seconds
Speed: 30 seconds per plate

Sealing Plate Height: 8-46 mm
Foil Length per plate: 119-128 mm
Sealing Temperature Range: ambient to 199 DegC
Sealing Time: 0.5-9.9 seconds
Speed: 30 seconds per plate