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![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | International Shipping |
![]() | Customer References Available |
![]() | Training and Installation Options Available |
- Stock: In Stock
- Brand: Thermo Scientific
- Product Code: 58238
- Condition: Pre-Owned
For Sale Thermo Scientific Invitrogen BIGFOOT Spectral Cell Sorter (4)Lasers/(25)Fluorescence Channels/(27) Detectors
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- WARRANTY
Here is what you will receive:
- Bigfoot Cell Sorter
- Computer running SQS software
- Communication and Power Cables
- Sorting alignment plates (Tubes, 96 well Plate, 384 well Plate)
- 100 μm nozzle installed
- (1) 1.5mL Tube sample holder
- (1) 5mL Tube sample holder
- (1) 15mL Tube sample holder
- (1) 50mL Sort output adapter
- (2) 15mL Sort output adapters
- (6) 5mL Sort output adapters
- (2) 1.5mL Sort output adapters
- (1) Sort output Loader Box
- (1) Sort output Microplate Stage
Bigfoot Cell Sorter Configuration:
Optics:
- Excitation lasers: Violet (405 nm), Blue (488 nm), Yellow-green (561 nm), Far red (640 nm)
- Laser/Channel Count: 7V-7B-7Y-4R
- Detection parameters: 25 fluorescence + 2 scatter
- Fluorescence Channels:
- Violet (405 nm): 420/10, 465/22, 525/35, 583/30, 661/20, 720/60, 750LP
- Blue (488 nm): 507/19, 549/15, 615/24, 670/30, 720/60, 750LP, 583/30
- Yellow-green (561 nm): 575/15, 589/15, 615/24, 661/20, 700/13, 720/24, 750LP
- Far red (640 nm): 670/30, 700/13, 728/40, 750LP
- Scatter parameters: standard FSC and SSC 488 nm
- Pulse measurement: simultaneously measured peak, area, and width for sample input and output of every channel
- Fluorescence sensitivity: <100 MESF for FITC, PE, APC
Fluidics:
- Nozzles: 100 μm ceramic nozzle, 30 PSI
- Sample input tube types: 1.5 mL, 5 mL, and 15 mL
- Temperature control: 4 DegC to 37 DegC for input and output of all media types
- Agitation: automatic based on input tube type
Sorting:
- Event rate: >100,000 events/sec acquisition with all 60 parameters
- Sorting speed: >70,000 events/sec with all parameters enabled
- Sort devices:
- sort output adapters for 1.5 mL, 5 mL, 15 mL, and 50 mL tubes
- straight-down sorting into 96-well plates
- straight-down sorting into 384-well plates
- 4-way sorting into 96-well plates
- 4-way sorting into 384-well plates
- Dimensions (WxDxH): 99 x 99 x 170 cm (39 x 39 x 67 in.)
- Weight: 1433 lbs (650 kg)
- Power supply: 100-240VAC 50/60Hz
Customization/Options: N/A
Testing performed by our in-house service team:
The Bigfoot Cell Sorter passed all tests and was found to be fully functional:
- We powered up the instrument and computer system.
- We verified that the instrument performed full startup and shutdown sequences.
- We performed extensive cleaning of the fluidic system.
- We adjusted the fluidic sensors and verified proper operation and flow.
- We tested the droplet generation function.
- We checked the laser powers and stable output.
- We performed alignment of the optics and lasers.
- We passed the QC bead alignment tests.
- We adjusted the stream collectors and verified the drop delay by testing.
- We verified the automatic stream alignment.
- We tested the media calibration wizard for the 5 mL tubes.
Instrument Overview:
- The Thermo Scientific Invitrogen BIGFOOT Cell Sorter offers high-speed, customizable cell sorting.
- It features 4 lasers and 27 detection channels for complex multicolor panels.
- BIGFOOT Cell Sorter ensures precise droplet generation and automatic stream alignment.
- The system includes advanced calibration wizards for consistent sorting performance.
- Robust design supports full startup, shutdown, and QC calibration sequences.
- Thermo Scientific BIGFOOT Cell Sorter is engineered for reliable, high-throughput operation.
Instrument Application:
- Immunophenotyping
- Rare cell isolation
- Stem cell sorting
- Cancer research
The Key Features:
- 4 lasers, 27 detection channels
- High-speed sorting
- Automated calibration and drop delay
- Spectral unmixing and conventional modes
- Multi-way sorting into various plate/tube formats
Additional technical info:
- Datasheet: Bigfoot Cell Sorter Datasheet
- Brochure: Bigfoot Cell Sorter Brochure
- Manual: Bigfoot Cell Sorter Manual
Video Overview:
About us
How we pack
VIEW SOME OF THE CUSTOMERS WE HELPED HERE
- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
- Warranty
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
1600#
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Bigfoot Cell Sorter?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards..
- We powered up the instrument and computer system.
- We verified that the instrument performed full startup and shutdown sequences.
- We performed extensive cleaning of the fluidic system.
- We adjusted the fluidic sensors and verified proper operation and flow.
- We tested the droplet generation function.
- We checked the laser powers and stable output.
- We performed alignment of the optics and lasers.
- We passed the QC bead alignment tests.
- We adjusted the stream collectors and verified the drop delay by testing.
- We verified the automatic stream alignment.
- We tested the media calibration wizard for the 5 mL tubes.
What supplies do I need?
You will need the consumables starting out:
- Bigfoot™ Calibration Beads | Thermo Fisher Scientific
- Cell Sorter Accessories | Thermo Fisher Scientific
Can I view the Bigfoot Cell Sorter in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Bigfoot Cell Sorter used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We powered up the instrument and computer system.
- We verified that the instrument performed full startup and shutdown sequences.
- We performed extensive cleaning of the fluidic system.
- We adjusted the fluidic sensors and verified proper operation and flow.
- We tested the droplet generation function.
- We checked the laser powers and stable output.
- We performed alignment of the optics and lasers.
- We passed the QC bead alignment tests.
- We adjusted the stream collectors and verified the drop delay by testing.
- We verified the automatic stream alignment.
- We tested the media calibration wizard for the 5 mL tubes.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a Bigfoot Cell Sorter on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Bigfoot Cell Sorter Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend tens of thousands of dollars and receive a damaged instrument.
Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing.
We generally ship parcels either via UPS or via freight for all larger instruments.
Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant). On freight shipments, we also use dense foam, which provides excellent cushioning and support for the instrument. Instruments then are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provide cushioning in all directions and hold instruments firmly in place.
Final step is to we photograph everything inside the crate and then seal it with screws.