Molecular Devices ImageXpress Micro Confocal High-Content Imaging System w/ Environmental Control, Water Immersion & Fluidics Options

For Sale Molecular Devices ImageXpress Micro Confocal High-Content Imaging System w/ Environmental Control, Water Immersion & Fluidics Options

 This item has been recently sold. Please view the other available instruments on your right.  

 If you do not see what you need, let us know via the “Request Quote” form; we often replenish sold-out items.

• Fully Functional
• Serviced & in Stock
• Current Model
• Good cosmetic condition
• Technical sales consultation available
•  WARRANTY

Here is what you will receive:

• Molecular Devices ImageXpress Micro Confocal Custom Upgraded High-Content Imaging System
• 96-well Fluidics Option
• Custom LED Transmitted Light Option
• Environmental Control Option
• Integrated image autofocus Option
• Robo EC Stage Option
• Spinning disk geometry: dual spinning disk with 50/250 um & 50/500 um pinhole
• Upgraded Lumencor Celesta 7 Laser Light Engine (Class 4 Lasers)
• Water Immersion Module
• Single Slide Holder
• Emission and Dichroic Filter Wheel Removal Tools
• Nikon Objectives:

- 4x/0.20 Plan APO Lambda

- 10x/0.45 Plan APO Lambda

- 20x/0.95 Water APO Lambda

- 60x/1.2 Water Plan APO VC

• Emission Filters: DAPI. FITC, CY3, TRITC, Texas Red, CY5, CY7
• 452/45, 483/32, 520/28, 562/40, 595/31, 624/40, 692/40, 819/44
• Computer running ImageXpress 6.7.2 software w/ dongle (w/ monitor and mouse)
• Communication and power cables

ImageXpress Micro Confocal HCI System Configuration:

• Upgraded Camera: PCO Edge 26 Monochrome sCMOS
• Resolution: 5120 x 5120 pixels
• High-throughput dual disk configuration with 50/250 um & 50/500 um confocal pinhole, and widefield modes
• Four-position objective changer
• Objectives:
• 4x/0.20 Plan APO Lambda
• 10x/0.45 Plan APO Lambda
• 20x/0.95 Water APO Lambda
• 60x/1.2 Water Plan APO VC
• 5-position software selectable dichroic filter wheel
• 8-position software selectable emission filter wheel
• Emission Filters installed: DAPI. FITC, CY3, TRITC, Texas Red, CY5, CY7
• High-speed laser autofocus with integrated image autofocus option
• Linear encoded voice coil-driven X, Y, and Z stages with better than 25 nm resolution
• Sample compatibility: slides and up to 1536-well microplates, round or flat bottom, low to high profile
• Throughput: >160K wells/day confocal, >200K wells/day widefield

Installed OPTIONAL system features:

• Environmental Control:

- Multi-day, live cell time-lapse imaging

- Provides appropriate atmospheric conditions (e.g., 5% or 10% CO2; requires premixed gas)

- Mimics physiological environment (30-40 DegC ± 0.5 DegC)

- Controls humidity and minimizes evaporation (0.5 uL/well/hour for 96- or 384-well formats)

- Compound Plate Temperature setting is installed along with the Fluidics option

• Water Immersion Option:

- Water-immersion controller maintains a stable water layer for high-NA imaging of live or thick samples.

- Water-immersion objectives (20x/0.95, 60x/1.2) improve resolution and reduce aberrations in aqueous 3D specimens.

• Fluidics Option:

- The robot is capable of dispensing volumes between 3 uL and 200 uL with an accuracy of ±5% or ±1 uL, whichever is greater.

Software Modules:

MetaXpress 6.7 Acquisition and Offline Analysis Software included foundational tools:

• Time-series acquisition and analysis

  • Integrated with Acquisition and Analysis Wizards

• Z-series acquisition

  • Acquisition integrated with the Acquisition Wizard

• Z-series analysis

  • Analysis integrated into the workflow

  • Compatible with MetaXpress PowerCore software

• Customized analysis

  • Integrated Custom Module Editor

• Transmitted light segmentation

  • Simple step cards are available with the Custom Module Editor

• Asynchronous kinetics

  • Built into the Acquisition Wizard

• Basic curve fitting

  • Included with the MetaXpress software

• Peak analysis

  • Included with the MetaXpress software

• Basic 3D acquisition setup tools

  • Includes Z-stack capture, 2D projection options

  • Interactive GUI for Z settings

Included OPTIONAL application modules:

Customization/Options:

• Other objectives might be available, please inquire with our sales team.

Testing performed by our in-house service team:

The ImageXpress Micro Confocal HCI System passed all tests and was found to be fully functional:

• We initialized the ImageXpress Micro Confocal System.

• We established communication with the software.
• We checked the clarity of the objectives.
• We tested the laser autofocus functionality.
• We ensured clarity in all installed filters.
• We verified the functionality of the Lumencor Celesta Light Engine
• We verified the functionality of the transmitted light LED.
• We performed multi-well acquisitions.
• We performed multi-channel slide acquisitions with image stitching.
• We tested the emission and dichroic filter wheel functionality.
• We tested the water immersion functionality.
• We performed fluidics positioning calibration.
• We tested fluid dispensing.
• We conducted spatial calibration.
• We confirmed camera clarity and functionality.
• We verified full X and Y stage movement.
• We confirmed the full Z movement.

Instrument Overview:

• The ImageXpress Micro Confocal High-Content Imaging System features a proprietary AgileOptix™ spinning-disk confocal optical path for high sensitivity and rapid imaging.
• The system supports water-immersion objectives (up to ~1.2 NA) to reduce aberrations and enhance imaging of thick tissues or 3D models.
• It offers environmental control (temperature, humidity, CO₂) to maintain live-cell conditions during long-term time-lapse experiments.
• Optional on-board fluidics modules enable automated liquid handling, media exchange and compound addition integrated with imaging workflows.
• Throughput can exceed ~160 000 wells per day in confocal mode and ~200 000 wells per day in widefield mode due to large field-of-view and high-speed acquisition.
• The design enables switching between widefield and confocal modalities within the same instrument to accommodate diverse sample types from slides to 1536-well plates.

Instrument Application:

• Long-term live-cell imaging of 3D spheroids or organoids under controlled temperature, CO₂, and humidity
• High-resolution imaging of thick samples using water-immersion objectives to reduce optical aberrations
• Automated compound addition or media exchange during time-lapse studies using integrated fluidics

The Key Features:

• Widefield and confocal imaging in one system
• Dual spinning-disk for high sensitivity
• Large field-of-view captures whole wells efficiently
• Water-immersion objectives reduce aberrations
• Built-in fluidics for automated media or reagent handling
• Environmental control ensures stable live-cell imaging

Additional technical info:

• Quick Start Guide: ImageXpress Micro Confocal Quick Start Guide
• Manual: ImageXpress Micro Confocal Manual

Video Overview:

About us

How we pack

 VIEW SOME OF THE CUSTOMERS WE HELPED HERE 

WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER IMAGING SYSTEMS FROM US?

  They want to ensure that internal optics are properly cleaned.

  They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned.

  They want to ensure that image quality is verified at the sample point for field distortion and uniformity.

  They want assurance that the motorized sample stage is responsive and accurately positions microplates and slides for repeatable image capture, avoiding well misalignment or incomplete scan coverage.

  They want to confirm excitation sources (LED, laser, or arc lamp) have been tested for power stability and wavelength accuracy, ensuring consistent fluorescence signal intensity across experiments.

  They want to ensure both brightfield and fluorescence imaging modes produce uniform, high-contrast, artifact-free images, verified through multi-mode scans and visual QC overlays.

  They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

COMMON QUESTIONS AND ANSWERS BELOW