Molecular Devices ImageXpress Micro Confocal HT.ai High-Content Laser Imaging System Including Robot w/ Environmental Control, Water Immersion & Transmitted Light Options

For Sale Molecular Devices ImageXpress Micro Confocal HT.ai High-Content Laser Imaging System Including Robot w/ Environmental Control, Water Immersion & Transmitted Light Options 

• Fully Functional
• Serviced & in Stock
• Current Model
• Good cosmetic condition
• Technical sales consultation available
•  WARRANTY
•   Optional on-site installation available

Here is what you will receive:

• Molecular Devices ImageXpress Micro Confocal HT.ai High-Content Imaging System
• ImageXpress power and options controller
• Installed emission Filters: DAPI, FITC, CFP, Texas Red, CY5, CY7, YFP
• Nikon Objectives: 

- 4x/0.20 Plan Apo

- 10x/0.45 Plan Apo Lambda

- 20x/0.95 Water Apo Lambda S LWD

- 40x/1.15 Water Apo Lambda S LWD

• 89 North LDI-NIR EX light engine (Class 4 Laser)
• Wave Lengths: 405, 445, 470, 640, 520, 555, 730
• Nikon Illumination arm with Nikon 100W Halogen lamp (D-LH/LC)
• Live Cell Instrument FC-9 Gas Mixer
• Water Immersion Control Box
• Single Slide Holder
• Computer running MetaXpress 6.7.2 with license dongle
• Automation Computer running Biosero Green Button Go v7.1.4
• Brooks PreciseFlex 400 sample handler robot
• (4) Molecular Devices 20-position plate hotels
• Barcode Tray position and swap position
• Emergency Stop button
• Laboratory table with predefined mount points and mount hardware
• Monitor, mouse, keyboard
• Communication and power cables

ImageXpress Confocal HT.ai HCI System Configuration:

• Camera: Andor Zyla sCMOS
• Spinning disk geometry: 50 um slit and 60 um pinhole (dual disk)
• High-speed laser autofocus with integrated image autofocus option
• Light Source: 89 North LDI light engine & 100W Halogen lamp
• Linear encoded voice coil-driven X, Y, and Z stages with better than 25 nm resolution
• 4-position automated objective changer
• Objectives:

- 4x/0.20 Plan Apo

- 10x/0.45 Plan Apo Lambda

- 20x/0.95 Water Apo Lambda S LWD

- 40x/1.15 Water Apo Lambda S LWD

• 5-position software-selectable dichroic filter wheel
• 8-position software-selectable emission filter wheel
• Filters Installed: DAPI, FITC, CFP, Texas Red, CY5, CY7, YFP
• Sample compatibility: slides and one to 1536-well microplates, round or flat bottom, low to high profile
• Throughput: >160K wells/day confocal, >200K wells/day widefield

Brooks PreciseFlex 400 Configuration:

• Configured Options: 750mm Z travel and Extended Reach Arm
• Range of motion & resolution:
• - J1 (Z) axis: 750mm
• - J2 axis: ± 90 degrees
• - J3 axis: ± 167 degrees
• - J4 axis: +/- 970 degrees
• Horizontal Reach (Extended Horizontal Reach, with included Servo Gripper) 734 mm
• Spring gripper force: 2-23 Newtons closing, 2-10 Newtons opening, 7 Newtons power off
• Resolution: 10 microns typical
• Repeatability: +/- 0.090 mm overall in x,y & z directions at 18-22C
• Maximum acceleration: 2500mm/sec² with 500 gram payload
• Maximum speed: 500 mm/sec Cartesian, 360 degrees/sec J2, 720 degrees/sec J3, J4
• Labware: SBS plates can be picked in landscape and portrait orientation.
• Weight: 30kg

Installed OPTIONAL System Features:

Environmental Control:

• Multi-day, live cell time-lapse imaging
• Provides appropriate atmospheric conditions. LCI FC-9 Gas Controller supports 5-10% CO₂
• Mimics physiological environment (30-40 DegC ± 0.5 DegC)
• Controls humidity and minimizes evaporation (0.5 μL/well/hour for 96- or 384-well formats)

Water Immersion Option:

• Water-immersion controller maintains a stable water layer for high-NA imaging of live or thick samples.
• Water-immersion objectives (20x/0.95, 40x/1.15) improve resolution and reduce aberrations in aqueous 3D specimens.

Transmitted Light Option:

• High-contrast imaging, where unstained cells are easily viewed or separated from the background
• Nikon Illumination arm with Nikon 100W Halogen lamp
• Ideal for non-fluorescent histochemically stained samples
• Fluorophore-independent morphology visualization with fluorescent imaging overlay

Software Modules:

MetaXpress 6.7 Acquisition and Offline Analysis Software included foundational tools:

• Time-series acquisition and analysis

  • Integrated with Acquisition and Analysis Wizards

• Z-series acquisition

  • Acquisition integrated with the Acquisition Wizard

• Z-series analysis

  • Analysis integrated into the workflow

  • Compatible with MetaXpress PowerCore software

• Customized analysis

  • Integrated Custom Module Editor

• Transmitted light segmentation

  • Simple step cards are available with the Custom Module Editor

• Asynchronous kinetics

  • Built into the Acquisition Wizard

• Basic curve fitting

  • Included with the MetaXpress software

• Peak analysis

  • Included with the MetaXpress software

• Basic 3D acquisition setup tools

  • Includes Z-stack capture, 2D projection options

  • Interactive GUI for Z settings

Included OPTIONAL Application Modules:

Customization/Options:

Other objectives might be available; please inquire with our sales team.

Testing performed by our in-house service team:

The ImageXpress Confocal HT.ai HCI System passed all tests and was found to be fully functional:

• We initialized the ImageXpress Micro Confocal System.
• We established communication with the software.
• We checked the clarity of the objectives.
• We tested the laser autofocus functionality.
• We ensured clarity in all installed filters.
• We tested the laser interlock safety shutoff.
• We verified the functionality of the 89 North LDI
• We performed multi-well acquisitions.
• We performed multi-channel slide acquisitions with image stitching.
• We tested the functionality of the emission and dichroic filter wheels.
• We conducted spatial calibration.
• We confirmed the operation of the FC-9 Gas Controller.
• We confirmed camera clarity and functionality.
• We verified full X- and Y-stage movement.
• We confirmed the full Z movement.

Brooks PreciseFlex 400 testing steps performed:

• We established communication with the Green Button Go for program development and testing.
• We established communication between the ImageXpress control PC and the Automation PC
• We executed the homing cycle, confirming accurate and consistent motion alignment.
• We ran multi-plate acquisitions to ensure smooth and reliable operation.
• We performed a full calibration to verify the precision of the system’s positioning.
• We tested collision detection and confirmed the system stopped immediately upon detection.
• We verified the emergency stop control.
• We monitored for joint position errors to ensure accurate joint movement and error-free operation.
• We assessed the joint envelope to validate the full range of motion and correct limits.
• We tested the gripper’s open/close functions, ensuring precise and responsive action.
• We checked the internal CMOS and servo encoder batteries, ensuring they functioned properly for consistent performance.

Instrument Overview:

• Molecular Devices ImageXpress Micro Confocal HT.ai delivers high-content confocal imaging with laser-based optical sectioning.
• Integrated environmental control supports stable live-cell imaging with regulated temperature, humidity, and CO₂.
• Water-immersion Nikon objectives provide high numerical aperture imaging for improved resolution and signal capture.
• The system includes transmitted-light imaging via a Nikon halogen illumination arm for structural visualization.
• Automated plate handling is enabled by a Brooks PreciseFlex 400 robot with multiple 20-position plate hotels.
• Designed for high-throughput, unattended operation, the platform supports fully automated imaging workflows.

Instrument Application:

• High-content imaging and quantitative analysis of live or fixed cells
• Multiplexed fluorescence assays for multi-parameter cellular readouts
• 3D imaging of spheroids, organoids, and complex tissue models
• High-throughput phenotypic screening for drug discovery
• Toxicity, viability, proliferation, and cell-health assessments
• Subcellular structure visualization and tracking over time
• Automated image acquisition for large plate formats and screening workflows

The Key Features:

• High-speed confocal imaging
• Widefield and confocal modes
• Environmental control
• Water-immersion optics support
• Multi-channel fluorescence

Additional technical info:

• Manual: ImageXpress Confocal HT.ai HCI System Manual
• Set Up Guide: ImageXpress Environmental Control Gas Mixer Set Up Guide
• Manual: PreciseFlex 400 Sample Handler Manual

Video Overview:

About us

How we pack

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Why do so many customers choose to order from us?

• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

*LA* Large Crate
790#

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