Imaging Systems and Gel Documentation

Capable Studies: Widefield, Brightfield, Confocal
Wavelength range: 7 class 3B lasers (405 nm/100 mW; 450 nm/75 mW; 488 nm/120 mW; 561 nm/100 mW; 594 nm/100 mW; 647 nm/120 mW; 785 nm/100 mW)
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanXApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Focus resolution: Sub-micron step size with advanced micro-stepping and double-stepping capabilities 

Andor Zyla sCMOS camera with single-disk spinning confocal optics (60 µm pinhole)
Laser and image-based autofocus with encoded XYZ stages offering <25 nm positioning resolution
Automated objective changer with 4×, 10×, 20× water, and 60× water immersion objectives
Supports slides and 1–1536 well plates; throughput >160K wells/day confocal imaging
Environmental control and water immersion modules

High-res CMOS camera with fast plate imaging
Fluorescence & white light modes, 4x objective
Supports 96/384-well SBS plates, rapid dispense
DispenCell S2022: low pressure, no calibration
Minimal prep, 5-min dispense, tracks cell size

Capable Studies: Widefield, Brightfield, Confocal
Illumination: 7-color laser light source for fluorescence excitation and a White LED for Brightfield
Objectives: 10x/0.40 UPlanSApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Camera: Photometrics X1
Live-cell imaging: HCA Onstage Incubator

High-res CMOS camera with fast plate imaging
Fluorescence & white light modes, 4x objective
Supports 96/384-well SBS plates, rapid dispense
DispenCell S2021: low pressure, no calibration
Minimal prep, 5-min dispense, tracks cell size

Capable Studies: Widefield, Brightfield, Confocal
Wavelength range: The Light Engine houses seven class 3B lasers (405 nm; 450 nm; 488 nm; 561 nm; 594 nm; 647 nm; 785 nm)
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanSApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Focus resolution: Sub-micron step size with advanced micro-stepping and double-stepping capabilities

Capable Studies: Widefield, Brightfield, Confocal
Illumination: 7-color laser light source for fluorescence excitation and a White LED for Brightfield
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanSApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Camera: Photometrics X1

Seeding: Nanoliter droplet dispensing (<1 psi) for fragile cell types
Imaging: Z-stack at 20 focal positions, 10× magnification, 650 nm laser
Clonality: Immediate single-cell check + daily growth imaging
Plate handling: automated heated stacker for up to 10 plates; Plate formats: 96-well standard; manual modes for 6–48 wells
Supplied with a VIPS Wash Station

Seeding: Nanoliter droplet dispensing (<1 psi) for fragile cell types
Imaging: Z-stack at 20 focal positions, 10× magnification, 650 nm laser
Clonality: Immediate single-cell check + daily growth imaging
Plate handling: Automated heated stacker for up to 10 plates
Plate formats: 96-well standard; manual modes for 6–48 wells

Andor Zyla sCMOS high-sensitivity camera
5-position filter changer with DAPI, FITC, TRITC, CY5 filters
4-position objective changer with Nikon Plan APO 4x/0.20, Plan APO Lambda 10x/0.45, Plan Apo 20x/0.75, Plan APO 40x/0.95 objectives
Plates supported: up to 1536-well plates and Slides with adapter
Widefield: yes

Imaging mode: Fluorescence, phosphor imaging, densitometry
Lasers: 488, 532, 635, 685, 785
Internal Filters: IP, Cy2, Cy3, Cy5; Loaded Filters: IR Short, IR Long
PMTs: Bi-alkali, Multi-alkali
Measurable dynamic range: >5 orders of magnitude

Capable Studies: Widefield, Brightfield
Wavelength range: (405 nm; 450 nm; 488 nm; 561 nm; 594 nm; 647 nm; 785 nm)
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanSApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Focus resolution: Sub-micron step size with advanced micro-stepping and double-stepping capabilities 

Low-pressure nanoliter droplet dispensing (< 1 psi) for gentle cell handling
Suitable for fragile and high-value cells (e.g., iPSCs, CHO cells, HEK293)
Z-stack imaging at 20 focal positions per well for enhanced depth resolution
Two-step clonality assurance
Automated in-line media dispensing immediately after seeding and imaging

Detection Modes: Fluorescence, Phosphorimaging, Digitization
Excitation Wavelengths: 473 nm (blue LD laser), 532 nm (green SHG laser), 635 nm (red LD laser)
Filters: LPG (O575), LPB (Y510), BPG1 (570DF20), LPR CH2 (R665)
Scanning Area: 40 x 46cm
Scan Pixel Sizes: 10, 25, 50, 100, 200um; Pre-Scan Pixel Size: 1000um

Version: NyOne 4K (Basic)
Illumination: LED Brightfield
Objective: Olympus 10x/0.30 UPlanFLN
Camera type: Basler a2A4504-18um CMOS
Culture system: Microwell plates (6, 12, 24, 48, 96, 384), Microscope slides, and Culture dishes

Imaging Mode: Phosphor Imaging
Laser: 635nm 84mW
Internal Filter: IP (390BP)
PMT: Bi-alkali
Measurable Dynamic Range: >5 orders of magnitude

Capable Studies: Widefield, Fluorescence, Brightfield
Wavelength range w/ LED, solid-state five-color light engine (386-23nm, 485-20nm, 560-25nm, 650-13nm, 740-13nm)
Objective: 10x/0.40 UPlanApo
Camera: Photometrics X1
Microplate Types: clear bottom 6-well through 1536 microwell plate, slides with insert adapter (not included)

Transmission source: 740 nm LED for Brightfield & Digital Phase Contrast
4 LED Light Sources
Emission Bandpass Filters: 430-500 nm, 655-760 nm, 570-650 nm, and 500-550 nm (for image alignment)
Objective Lenses: 5x, 10x, 20x Air
Camera: high-sensitivity 4.7 Mp sCMOS camera

Camera: Andor Zyla sCMOS camera
Speed: >200,000 wells/day
5-position filter cube changer; 4 position objective changer
Plates supported: up to 1536-well plates
96-well plate scan time, 2 colors, 1 site/well: 2 minutes

Camera: >4 megaPixel sCMOS
Speed: >200,000 wells/day
5-position filter cube changer; 4 position objective changer
Plates supported: up to 1536-well plates
96-well plate scan time, 2 colors, 1 site/well: 2 minutes

Imaging Modes: Brightfield, Fluorescence (Green)
Fluorescence: Green Excitation (470/40x) Emission (510lp)
Objective Lens: 2x
Light Source: LED
Camera: 5MP CMOS

Illumination: transmitted and RGB transmitted modes
Objective capacity: 4-position manual turret with 4x, 10x, 20x phase contrast objectives
Condenser: 4-position turret with clear aperture and 3-phase annuli
Camera: 3.2 MP CMOS (2064 × 1536), 3.45 µm pixel resolution
Fluorescence: 2-position chamber

Automated plate handling within a temperature-controlled stacker for up to 10 plates (96-well)
Manual scan mode formats: 6, 12, 24, 48-well plates
Laser wavelength: 650nm
Max average power: 850uW
Data Storage: Whole plate image: appr 150MB

5-position manual objective turret with 4x, 10x, and 20x lenses.
Phase contrast ring with brightfield and multiple phase options.
Filter cubes for DAPI, GFP, and RFP channels.
Supports transmitted and RGB transmitted light illumination.
Mechanical X/Y stage for precise sample positioning.

5-position manual objective turret with 4x, 10x, and 20x lenses.
Phase contrast ring with brightfield and multiple phase options.
Filter cubes for DAPI, GFP, and RFP channels.
Supports transmitted and RGB transmitted light illumination.
Mechanical X/Y stage for precise sample positioning.