Imaging Systems

Andor Zyla sCMOS camera with dual-disk spinning confocal optics (50 µm slit, 60 µm pinhole)
Laser and image-based autofocus with encoded XYZ stages offering <25 nm positioning resolution
Automated objective changer with 4×, 10×, 20× water, and 40× water immersion objectives
Supports slides and 1–1536 well plates; throughput >160K wells/day confocal imaging
Environmental control, water immersion, and transmitted light imaging modules

Capable Studies: Widefield, Brightfield, Confocal
Wavelength range: 7 class 3B lasers (405 nm/100 mW; 450 nm/75 mW; 488 nm/120 mW; 561 nm/100 mW; 594 nm/100 mW; 647 nm/120 mW; 785 nm/100 mW)
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanXApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Focus resolution: Sub-micron step size with advanced micro-stepping and double-stepping capabilities 

PCO Edge 26 sCMOS camera with 5120 × 5120 resolution
Dual spinning-disk confocal geometry with 50/250 µm and 50/500 µm pinholes
Four-position objective changer with 4x, 10x, 20x/0.95 water, 60x/1.2 water objectives
5-position dichroic and 8-position emission filter wheels with full DAPI–CY7 set
Environmental Control, Water Immersion, Fluidics Option

High-res CMOS camera with fast plate imaging
Fluorescence & white light modes, 4x objective
Supports 96/384-well SBS plates, rapid dispense
DispenCell S2022: low pressure, no calibration
Minimal prep, 5-min dispense, tracks cell size

Capable Studies: Widefield, Brightfield, Confocal
Illumination: 7-color laser light source for fluorescence excitation and a White LED for Brightfield
Objectives: 10x/0.40 UPlanSApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Camera: Photometrics X1
Live-cell imaging: HCA Onstage Incubator

Camera: Andor Zyla 4 megaPixel sCMOS
Throughput: >160K wells/day confocal, >200K wells/day widefield
4 position objective changer/ 5-position dichroic filter wheel/ 8-position emission filter wheel
Plates supported: up to 1536-well plates
Spinning disk geometry: 60 um pinhole & 42um pinhole (dual disk)

Capable Studies: Widefield, Brightfield, Confocal
Illumination: 7-color laser light source for fluorescence excitation and a White LED for Brightfield
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanSApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Camera: Photometrics X1

Andor Zyla sCMOS high-sensitivity camera
5-position filter changer with DAPI, FITC, TRITC, CY5 filters
4-position objective changer with Nikon Plan APO 4x/0.20, Plan APO Lambda 10x/0.45, Plan Apo 20x/0.75, Plan APO 40x/0.95 objectives
Plates supported: up to 1536-well plates and Slides with adapter
Widefield: yes

Capable Studies: Widefield, Brightfield, Confocal
Wavelength range: wavelengths between 386 nm–747 nm
Microplate Types: clear bottom 96-, 384-, and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.30 UPlanFLN objective, 20x/0.50 UPlanFLN objective, 4x/0.13 UPlanFL objective, (3 position objective changer)
Live-cell imaging: HCA Onstage Incubator

Capable Studies: Widefield, Brightfield
Wavelength range: (405 nm; 450 nm; 488 nm; 561 nm; 594 nm; 647 nm; 785 nm)
Microplate Types: clear bottom 96-, 384- and 1536-, slides as well with insert adapter (up to 4 glass microscope slides)
Objectives: 10x/0.40 UPlanSApo, 20x/0.45 LUCPlanFL N, 40x/0.60 LUCPlanFL N (3 position objective changer)
Focus resolution: Sub-micron step size with advanced micro-stepping and double-stepping capabilities 

Imaging Modes: Widefield (open aperture), Confocal (IRIS, EDGE line scanning), Transmitted light Light
Source: four laser lines (405, 488, 561, 642) White LED for transmitted light
Microplate Types: clear bottom 6 - 1536 well, slides with insert adapter (up to 4 glass microscope slides), and 35mm/40mm petri dish
Objectives: 4x/.20 Plan Apo, 10x/0.45 Plan Apo, 20x/0.75 Plan Apo, 40x/1.15 Apo S LWD Water (4 position objective changer)
Liquid Handling Module and Live Cell Imaging Module options

Low-pressure nanoliter droplet dispensing (< 1 psi) for gentle cell handling
Suitable for fragile and high-value cells (e.g., iPSCs, CHO cells, HEK293)
Z-stack imaging at 20 focal positions per well for enhanced depth resolution
Two-step clonality assurance
Automated in-line media dispensing immediately after seeding and imaging

5-position manual objective turret with 4x, 10x, and 20x lenses.
Phase contrast ring with brightfield and multiple phase options.
Filter cubes for DAPI, GFP, and RFP channels.
Supports transmitted and RGB transmitted light illumination.
Mechanical X/Y stage for precise sample positioning.

Detection Modes: Fluorescence, Phosphorimaging, Digitization, Chemiluminescence
The Fluor stage is included for Fluorescence imaging
Filters: BPG1, BPB1, LPR (Ch2), IP (Ch1)
Excitation Wavelengths: 473nm (blue LD laser), 532nm (green SHG laser), 635nm (red LD laser)
Scan Pixel Sizes: 10, 25, 50, 100, 200um

Transmission source: 740 nm LED for Brightfield & Digital Phase Contrast
4 LED Light Sources
Emission Bandpass Filters: 430-500 nm, 655-760 nm, 570-650 nm, and 500-550 nm (for image alignment)
Objective Lenses: 5x, 10x, 20x Air
Camera: high-sensitivity 4.7 Mp sCMOS camera

Camera: Andor Zyla sCMOS camera
Speed: >200,000 wells/day
5-position filter cube changer; 4 position objective changer
Plates supported: up to 1536-well plates
96-well plate scan time, 2 colors, 1 site/well: 2 minutes

Camera: >4 megaPixel sCMOS
Speed: >200,000 wells/day
5-position filter cube changer; 4 position objective changer
Plates supported: up to 1536-well plates
96-well plate scan time, 2 colors, 1 site/well: 2 minutes

Camera: CMOS, Field of view 10x: 1.92 mm2
Six-position objective changer/filter cube turret
Supported labware: 6- to 384-well plates and 25 mm x 75 mm (1 in. x 3 in.) slides, 35 mm culture dishes
96-well plate scan time, two colors, one site/well: 8 minutes
Temperature control: Without environmental control cassette 6°C (11°F) above ambient up to 40°C (104°F)

Low-pressure nanoliter droplet dispensing (< 1 psi) for gentle cell handling
Suitable for fragile and high-value cells (e.g., iPSCs, CHO cells, HEK293)
Z-stack imaging at 20 focal positions per well for enhanced depth resolution
Automated in-line media dispensing immediately after seeding and imaging

Low-pressure nanoliter droplet dispensing (< 1 psi) for gentle cell handling
Suitable for fragile and high-value cells (e.g., iPSCs, CHO cells, HEK293)
Z-stack imaging at 20 focal positions per well for enhanced depth resolution
Automated in-line media dispensing immediately after seeding and imaging

Automated plate handling within a temperature-controlled stacker for up to 10 plates (96-well)
Manual scan mode formats: 6, 12, 24, 48-well plates
Laser wavelength: 650nm
Max average power: 850uW
Data Storage: Whole plate image: appr 150MB

Automated plate handling within a temperature-controlled stacker for up to 10 plates (96-well)
Manual scan mode formats: 6, 12, 24, 48-well plates
Laser wavelength: 650nm
Max average power: 850uW
Data Storage: Whole plate image: appr 150MB

Imaging Methods: single color, multicolor, time-lapse, Z-stacking, and movie capture
Fluorescence Light Path: Motorized LED cube interchange mechanism.
Objectives: Evos LPlanFL PH2 4x/0.13, 10x/0.3, 20x/0.4, 40x/0.65 (5-position manual turret)
Filter Cubes: DAPI (357/447 nm), GFP (482/524 nm), Texas Red (585/628 nm)
High-sensitivity 3.2 MP monochrome CMOS camera (2,048 x 1,536 pixels) with 3.45 μm pixel resolution