Waters ACQUITY UPLC H-Class PLUS Bio System w/ PDA and FLR Detectors (THIS IS A PLUS MODEL, MADE IN 2021)

For Sale Waters ACQUITY UPLC H-Class PLUS Bio System w/ PDA and FLR Detectors

• Fully Functional
• Serviced & in Stock
• Current Model
• Made in 2021 (MINIMAL USE)
• Good cosmetic condition
• Technical sales consultation available
• WARRANTY

  Here is what you will receive:

• Acquity UPLC H-Class PLUS BioQuaternary Solvent Manager ACQ-QSM: REF 186015081 / SN E21QBP288M
• Acquity UPLC H-Class PLUS BioSample Manager ACQ-FTN-H w/ 50µL sample loop: REF 186015086 / SN E21FBP332M
• Acquity UPLC Column manager: REF 186015043 / SN C21CMP892G
• Acquity UPLC Column manager AUX: REF 186015049 / SN D21CMX201G
• Acquity UPLC Detector ACQ-PDA with analytical flow cell 1000 psi max, 500nL: REF 186015032 / SN C21UPD490A
• Acquity UPLC Detector ACQ-FLR with analytical flow cell 500 psi max, 2µL: REF 186015029/ SN F20UPF671G
• PC with Empower software SW 
• Solvent tray
• Inter-communication cables
• Standard power cords

ACQUITY UPLC H-Class PLUS Bio System Configuration:

BioQuaternary Solvent Manager:

• Blend up to four solvents in any combination (standard).
• Expanded solvent choices with optional six-port solvent select valve.
• Integrated vacuum degassing with four chambers.
• One additional chamber for the SM-FTN-H purge solvent.
• Automated on-line pH, ionic strength, and organic modifier blending from pure solvents (Auto-Blend Plus™ Technology).
• Low-pressure mixing, quaternary gradient formation.
• 11 gradient curves including linear, step (2), concave (4), and convex (4).

BioSample Manager-FTN (bioSM-FTN-H):

• Injection volume range: 0.1 to 10.0 µL standard, up to 1,000.0 µL with optional extension loops (not included)
• Equipped with a 50 µL sample loop for standard injection operations.
• Accuracy (aspiration): ±0.2 µL, based on 10 µL injections averaged over 20 injections using a standard 100 µL syringe.
• Linearity: ≥0.999 using a standard needle.
• Precision: ≤1% RSD (0.2 to 1.9 µL), ≤0.5% RSD (2.0 to 4.9 µL), ≤0.25% RSD (5.0 to 100.0 µL).
• Sample plate options(any 2 of the following): 96 and 384 microtiter plates, or vial and micro‑centrifuge tube plate formats.
• Sample plate configuration: 2 × 48‑position 2.00‑mL vial plates.
• Maximum sample capacity: up to 768 samples (two 384‑well plates or 96 two‑mL vial holders).
• Temperature control range: 4.0 to 40.0 °C, adjustable in 0.1 °C increments.
• Temperature performance: ±0.5 °C accuracy and ±1.0 °C stability at sensor.
• Sample handling features: integrated needle wash, low residual volume (3 µL), and minimal carryover (≤0.002%).

Acquity UPLC PDA Detector:

• Wavelength range: 190 to 500 nm
• Wavelength accuracy: ±1 nm
• Linearity range: deviation at 2.0 AU ≤5%
• Optical resolution: 1.2 nm
• Digital resolution: 0.6 nm/pixel to 500 nm
• Sampling rate: up to 80 points/s
• Light source: deuterium lamp
• Flow cell design (analytical): light-guiding UPLC intelligent technology flow cell
• Flow cell volume: 500 nL
• Path length: 10 mm
• Pressure limit: 1000 psi (all flow cells)

Acquity UPLC FLR Detector:

• Wavelength range: Ex.:200 to 890 nm; Em.: 210 to 900 nm
• Wavelength accuracy: ±3 nm
• Bandwidth: 20 nm
• Wavelength repeatability: ±0.25 nm
• Sensitivity: S/N >1000 (Raman spectrum of H2O)
• Measurement range: 0.001 to 10,000 EU
• Data acquisition: up to 80 Hz
• Flow cell design: axially illuminated
• Cell volume: <2 uL
• Pressure limit: 500 psi

Acquity UPLC CM-A Column Manager:

• Column capacity: 2 columns (max. length of 150 mm with filter or guard column) or four columns, using a CM-Aux stacked above a CM-A (max. length of 50 mm) with optional tubing kit, up to 4.6 mm (I.D.) 
• Switching valves: 2 nine-port, eight-position valves; provides programmable, automatic, random access switching, waste and bypass positions for rapid solvent changeover
• Column compartment temperature range: 4.0 to 90.0 DegC, settable in 0.1 DegC increments; 2 independent heat/cool zones per module, up to six zones in a stacked configuration
• Solvent conditioning: active pre-heating as standard

Customization/Options: 

• Other detectors and flow cell options might be available. Please inquire with our sales team. 

Testing performed by our in-house service team:

The ACQUITY UPLC H-Class PLUS Bio System passed all tests and was found to be fully functional:

Waters Acquity UPLC H-Class PLUS Bio QSM Quaternary Pump:

• We completed the initialization test.
• We verified software communication.
• We assessed the gradient proportioning valve performance.
• We evaluated the seal washing pump operation.
• We tested the leak sensor functionality.
• We conducted a degasser health check.
• We verified the pressure limiter operation.
• We measured flow rate accuracy.
• We assessed solvent delivery stability.
• We performed a leak test.
• We executed the gradient test.

Waters Acquity UPLC H-Class PLUS Bio Sample Manager FTN-H:

• We completed the initialization test.
• We verified software communication.
• We evaluated the sample cooler set temperature accuracy.
• We performed the syringe leak test.
• We assessed the needle seal integrity.
• We checked the leak detector functionality.
• We conducted the reproducibility test.

Waters Acquity UPLC Column Manager:

• We powered on and ensured proper initialization of the column manager.
• We checked communication with the Empower software.
• We tested column cooling and heating
• We performed a column oven temperature accuracy test to verify the accuracy of temperature control in the column oven.

Waters Acquity UPLC PDA Detector:

• We inspected the detector for physical damage.
• We performed extensive checks to evaluate the proper initialization of the PDA detector.
• We checked the seamless communication with the Empower software.
• We monitored the remaining lamp life to ensure optimal performance. (Lamp hours: 44HR)
• We ran tests to verify accurate and reliable detection capabilities.
• We performed leak-sensor testing in the fluidic pathways and components to verify the detector's accuracy and reliability.

Waters Acquity UPLC Fluorescence (FLR) Detector:

• We inspected the Waters Acquity UPLC FLR Detector for physical damage.
• We checked the seamless communication with the software.
• We checked the remaining life of the lamp to ensure proper functionality. (Lamp hours: 276 Hr)
• We verified calibration accuracy.

Instrument Overview:

• High-performance UHPLC platform designed for robust bioseparations and flexible workflow integration.
• Supports quaternary solvent mixing with precise gradient formation and reliable flow control.
• Equipped with PDA and FLR detectors for complementary UV-Vis and fluorescence detection capabilities.
• Optimized for biocompatible workflows with inert flow path materials and low-sample adsorption.
• Provides accurate temperature control and consistent solvent management for reproducible separations.
• Delivers high sensitivity, resolution, and throughput for complex biomolecule analysis.

Instrument Application:

• Monoclonal antibody characterization and purity assessment
• Glycan profiling and protein heterogeneity analysis
• Peptide mapping and biomolecule structural confirmation
• Aggregation and stability studies of biologics
• Fluorescence-based detection of labeled biomolecules

The Key Features:

• UHPLC for bioseparations
• Quaternary solvent mixing
• PDA and FLR detection
• Biocompatible inert flow path
• Precise gradient control
• High sensitivity, resolution

Additional technical info:

• Manual: ACQUITY UPLC H-Class Bio System Manual
• Manual: Acquity Column Manager Manual
• Manual: Waters Acquity PDA Detector Manual
• Manual: Waters Acquity FLR Detector Manual 

Video Overview:

About us

How we pack

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Why do so many customers choose to order from us?

• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

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