Thermo Scientific Vanquish Flex Quaternary UHPLC System w/ Diode Array & Fluorescence Detectors Unit 5

For Sale Thermo Scientific Vanquish Flex Quaternary UHPLC System with Diode Array & Fluorescence Detectors Unit 5

 This item has been recently sold. Please view the other available instruments on your right.  

 If you do not see what you need, let us know via the “Request Quote” form; we often replenish sold-out items.

• Fully Functional
• Serviced & in Stock
• Current Model
• Made in 2022
• Good cosmetic condition
• Technical sales consultation available
•  WARRANTY

Here is what you will receive:

• Vanquish Fluorescence Detector F w/ Standard Bio Flow Cell 8uL, 20bar p/n: VF-D50-A
• Vanquish Diode Array Detector HL w/ Standard LightPipe flow cell, 2 uL, 10 mm, 6 MPa p/n: VH-D10-A
• Vanquish Split Sampler FT p/n: VF-A10-A
• Vanquish Split Sampler Sample Racks 54 pos. x 12 mm OD vials (≤ 1.5 mL) Qty.(4)
• Vanquish Quaternary Pump F w/ Static Mixer, volume: 350 uL & Capilary Mixer, volume: 50 uL p/n: VF-P20-A  
• Vanquish Column Compartment H w/ Active Column Preheater p/n: VH-C10-A
• Solvents Rack
• Inter-communication cables
• Standard power cords

Please note that the computer with Thermo Chromeleon software is not included.

You can purchase a Chlomeleon license for around $5,500.

Vanquish Flex Quaternary UHPLC System Configuration:

Vanquish Fluorescence Detector F:

• Optical design: two monochromators with concave holographic gratings and elliptic mirrors for the highest efficiency in light transmission
• Light source: xenon flash lamp (15W)
• Number of photomultipliers: 1
• Wavelength range: Excitation: 220 nm - 630 nm/Emission: 220 nm - 650 nm
• Spectral bandwidth: Excitation: 20 nm/Emission: 20 nm
• Wavelength accuracy: ± 2 nm
• Wavelength repeatability: ± 0.2 nm
• Spectra scanning modes: Single spectrum scan modes: Excitation, Emission, Synchronous
• Number of channels: up to 4 channels
• Data collection rate: up to 200 Hz
• Wavelength switching time: <250 ms
• Emission filter: variable: 5 positions
• Flow Cell: standard flow cell, biocompatible (8 uL, 2 MPa, fused silica)
• Flow cell thermostat 15 DegC above ambient to 50 DegC absolute

Vanquish Diode Array Detector HL:

• Optical design: single-beam, reverse-optics design with concave holographic grating, High Numerical Aperture (NA) achromatic optics, 1024 element photodiode array.
• Light source: deuterium lamp
• Wavelength range: 190–680 nm
• Spectral bandwidth: pixel resolution: 0.5 nm (average); optical resolution: 1 nm (FWHM with 1 nm slit width)
• Slit width Settable: settable: 1 nm, 2 nm, 4 nm, 8 nm
• Wavelength accuracy: ±1 nm
• Wavelength repeatability: ±0.1 nm
• Wavelength calibration: internal calibration with the D-alpha line of the deuterium lamp
• Wavelength validation: internal validation with holmium oxide filter
• Number of signal channels: up to 10 + 3D field
• Data collection rate: up to 200 Hz
• Noise: <±3 μAU at 230 nm
• Drift: <0.5 mAU/h at 230 nm
• Linearity: <5% at 2.0 AU (typically <5% at 2.5 AU)
• Flow cell: Standard LightPipe flow cell, biocompatible (2 uL, 10 mm, 6 MPa, fused silica)
• Flow cell pressure limit: 6 MPa (60 bar, 870 psi)

Vanquish Split Sampler FT:

• Injection units: 1
• Operating principle: split loop injection
• Pressure range: 2–103 MPa
• Injection volume range: 0.01–25 uL, min. Step = 0.01 uL
• Injection volume accuracy: typically ±0.5% for 10 uL water
• Injection volume precision: <0.25% area RSD for 1 uL (caffeine in water); typically <0.5% area RSD for 0.5 uL (caffeine in water)
• Injection linearity: r > 0.99999 (caffeine in water)
• Injection cycle time: down to 8s depending on separation conditions
• Minimum sample required: 2 uL at 1 uL injection volume
• Carryover (UV): <0.002% with caffeine (typically: <0.0004%)
• Needle wash (external): 1 solvent per injection unit, dip rinse and continuous rinse
• Sample compartment temperature range: 4–40 DegC (≥23 K below ambient at <80% RH)
• Sample temperature accuracy: -2 DegC/+4 DegC
• Sample temperature stability: ±1 DegC
• Dwell volume (contribution of the autosampler to the system gradient delay volume):
• Method Transfer capability: 110 uL with 25 uL sample loop
• Sample capacity: 4 segments useable for sample racks or well plates with SBS footprint format; plus a capacity of 12 x 22.5 mm O.D. vials in the carousel
• Included sample racks: (4) x 54×12 mm OD vials (≤1.5 mL)
• Barcode reading: empty segment detection, rack/well plate verification, inventory management

Vanquish Quaternary Pump F:

• Operating Principle: serial dual-piston pump
• Flow Range: 0.001–8 mL/min, in 1 uL/min increments
• Pressure Range: 2–103 MPa; flow rate >5 mL/min, the pressure range decreases 80 MPa
• Compressibility Compensation: fully automated, independent of the composition of the mobile phase
• Flow Accuracy: ±0.1%
• Flow Precision: <0.05% RSD or <0.01 min SD, whichever is greater
• Pulsation typically: <1.0% or <0.2 MPa, whichever is greater
• Gradient Formation: low-pressure gradient proportioning
• Proportioning Accuracy: ±0.5% of full-scale
• Proportioning Precision: <0.15% SD
• Number of Solvent Lines: 4
• Mixer Volume: 400 uL (50 uL proprietary capillary mixer and 350 uL static mixer)
• Dwell Volume: 679 uL 
• Solvent Degassing: built-in, 4 Channels

Vanquish Column Compartment H with Active Column Preheater:

Operating Principle Column Chamber: Still air and forced air

• Temperature Range: 5–120 DegC in 0.1 DegC increments (18K below ambient)
• Temperature Stability: ±0.05K
• Temperature Accuracy: ±0.5K (up to 80 DegC)
• Heating Performance: <15 min, 20–50 DegC, ±1K Typical: 5 min, 25–40 DegC, ±1K
• Cooling Performance: <15 min, 50–20 DegC, ±1K
• Capacity: 2 column slots

Operating Principle Pre-heater: Active

• Temperature Range: 30–120 DegC  (5K above ambient)
• Temperature Stability: ±0.5K
• Temperature Accuracy: ±2K
• Heating Performance: 80 DegC at 2.5 mL/min, 120 DegC at 1 mL/min (water/acetonitrile (50/50, v/v))
• Capacity: 2 column slots
• Temperature Zones: 1 per device (up to 3 zones in stacked configuration)
• Column ID: 4 column ID tags
• Valve: 2 Positions 6 Ports Thermo Scientific 150MPa bio

Customization/Options: N/A

Testing performed by our in-house service team:

The Vanquish Flex Quaternary UHPLC System passed all tests and was found to be fully functional:

Vanquish Quaternary Pump F:

• We performed the initialization test.
• We conducted the self-test.
• We verified the software communication test.
• We performed the gradient proportioning valve test.
• We completed the seal-washing pump test.
• We ran the pressure limiter test.
• We tested the flow rate accuracy.
• We ran a solvent delivery stability.

Vanquish Split Sampler FT:

• We conducted the initialization test.
• We checked the software communication.
• We tested the sample cooler set temperature accuracy.
• We performed the needle wash test.
• We carried out the reproducibility test.

Vanquish Column Compartment H:

• We conducted the initialization test.
• We verified the software communication.
• We conducted the heating and cooling test.
• We performed the switching valve test.
• We tested the active column preheater.
• We checked the leak sensor.

Vanquish Diode Array Detector HT:

• We performed the initialization test.
• We verified the software communication.
• We checked the leak sensor.
• We conducted test injections of the caffeine solution and verified the peaks.

Vanquish Fluorescence Detector F:

• We conducted the initialization test.
• We verified the software communication.
• We checked the leak sensor.
• We performed test injections of the test solution and verified the resulting peaks.

Instrument Overview:

• Thermo Scientific Vanquish Flex Quaternary UHPLC delivers high-pressure, high-resolution liquid chromatography.
• Supports quaternary solvent blending with precise gradient control for complex separations.
• Diode Array and Fluorescence Detectors enable broad spectral coverage and high-sensitivity detection.
• Modular design allows configuration flexibility for bioanalytical and pharmaceutical workflows.
• Offers consistent temperature control and sample integrity with advanced column and sampler options.
• Designed for high-throughput labs requiring robustness, reliability, and reproducible performance.

Instrument Application:

• Analysis of pharmaceutical impurities and degradation products
• Detection of fluorescently labeled biomolecules (e.g., proteins, peptides)
• Simultaneous quantification of vitamins in nutritional supplements
• Monitoring of polycyclic aromatic hydrocarbons (PAHs) in environmental samples
• Quality control of dye-labeled oligonucleotides in genetic research

The Key Features:

• Quaternary pump supports complex gradients
• DAD and FLD enable a broad detection range
• High-pressure capable of sharp peaks
• Precise temperature control for stability
• Modular setup suits varied workflows
• Low dispersion design ensures high resolution

Additional technical info:

• Manual: Thermo Vanquish UHPLC Manual
• Manual: Vanquish Fluorescence Detector Manual
• Manual: Vanquish Diode Array Detector Manual
• Manual: Vanquish Split Sampler FT Manual
• Manual: Vanquish Quaternary Pump F Manual
• Manual: Vanquish Column Compartment H Manual

Video Overview:

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Why do so many customers choose to order from us?

• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• An extended warranty may be available
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

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