Thermo Scientific Vanquish Flex Quaternary UHPLC System with Variable Wavelength and Fluorescence Detectors

For Sale Thermo Scientific Vanquish Flex Quaternary UHPLC System with Variable Wavelength and Fluorescence Detectors

• Fully Functional
• Serviced & in Stock
• Current Model
• Made in 2022-2023
• Good cosmetic condition
• Technical sales consultation available
•  WARRANTY
• Includes remote instrument setup and training session

Here is what you will receive:

• Vanquish Quaternary Pump F w/ Static Mixer, volume: 350 uL p/n: VF-P20-A
• Vanquish Split Sampler FT p/n: VF-A10-A
• Vanquish Split Sampler Sample Racks 54 pos.x12 mm OD vials (≤ 1.5 mL) Qty.(3)
• Vanquish Column Compartment H  p/n: VH-C10-A
• Vanquish Variable Wavelength Detector F p/n: VF-D40-A with Standard Bio flow cell (11 uL, 10 mm, 50Bar) p/n 6077.0200
• Vanquish Fluorescence Detector F, p/n: VF-D50-A with Standard Bio flow cell (8 uL,20Bar) p/n 6079.4230
• PC loaded with Chromeleon software v.7.3.1
• Solvents Rack
• Inter-communication cables
• Standard power cords

Vanquish Flex Quaternary UHPLC System Configuration:

Vanquish Fluorescence Detector F:

• Optical design: two monochromators with concave holographic gratings and elliptic mirrors for the highest efficiency in light transmission
• Light source: xenon flash lamp (15W)
• Number of photomultipliers: 1
• Wavelength range: Excitation: 220 nm - 630 nm/Emission: 220 nm - 650 nm
• Spectral bandwidth: Excitation: 20 nm/Emission: 20 nm
• Wavelength accuracy: ± 2 nm
• Wavelength repeatability: ± 0.2 nm
• Spectra scanning modes: Single spectrum scan modes: Excitation, Emission, Synchronous
• Number of channels: up to 4 channels
• Data collection rate: up to 200 Hz
• Wavelength switching time: <250 ms
• Emission filter: variable: 5 positions
• Flow Cell: standard flow cell, biocompatible (8 uL, 2 MPa, fused silica)

Vanquish Variable Wavelength Detector F:

• Optical design: UV/VIS/NIR photometer (tunable Czerny-Turner monochromator) with additional internal reference beam
• Light source: Deuterium lamp for UV and VIS; Tungsten lamp for VIS and NIR
• Wavelength range: 190–900 nm (with deuterium and tungsten lamp)
• Spectral bandwidth: typ. 6 nm at 254 nm
• Wavelength Accuracy: ± 1 nm
• Wavelength Repeatability: ± 0.1 nm
• Wavelength Calibration: Internal calibration with zero order of monochromator spectrum
• Wavelength Validation: Internal validation with holmium-oxide filter
• Signal channel: Up to 4 signal channels
• Data collection rate (single-channel): up to 250 Hz; (multi-channel) Up to 5 Hz
• Noise (single-channel): <±2.5 uAU at 254 nm
• Noise (multi-channel): <±10 uAU (typical <±7 uAU) at 254 nm and 280 nm
• Drift: <0.1 mAU/h at 254 nm
• Linearity: <5% at 2.5 AU (typical <3% at 2.5 AU
• Flow Cell: Standard Bio flow cell (11 uL, 10 mm, 50Bar)

Vanquish Quaternary Pump F:

• Operating Principle: serial dual-piston pump
• Flow Range: 0.001–8 mL/min, in 1 uL/min increments
• Pressure Range: 2–103 MPa; flow rate >5 mL/min, the pressure range decreases 80 MPa
• Compressibility Compensation: fully automated, independent of the composition of the mobile phase
• Flow Accuracy: ±0.1%
• Flow Precision: <0.05% RSD or <0.01 min SD, whichever is greater
• Pulsation typically: <1.0% or <0.2 MPa, whichever is greater
• Gradient Formation: low-pressure gradient proportioning
• Proportioning Accuracy: ±0.5% of full-scale
• Proportioning Precision: <0.15% SD
• Number of Solvent Lines: 4
• Mixer Volume: 350 uL static mixer
• Dwell Volume: 679 uL 
• Solvent Degassing: built-in, 4 Channels

Vanquish Split Sampler FT:

• Injection units: 1
• Operating principle: split loop injection
• Pressure range: 2–103 MPa
• Injection volume range: 0.01–100 uL, min. Step = 0.01 uL
• Injection volume accuracy: typically ±0.5% for 10 uL water
• Injection volume precision: <0.25% area RSD for 1 uL (caffeine in water); typically <0.5% area RSD for 0.5 uL (caffeine in water)
• Injection linearity: r > 0.99999 (caffeine in water)
• Injection cycle time: down to 8s depending on separation conditions
• Minimum sample required: 2 uL at 1 uL injection volume
• Carryover (UV): <0.002% with caffeine (typically: <0.0004%)
• Needle wash (external): 1 solvent per injection unit, dip rinse and continuous rinse
• Sample compartment temperature range: 4–40 DegC (≥23 K below ambient at <80% RH)
• Sample temperature accuracy: -2 DegC/+4 DegC
• Sample temperature stability: ±1 DegC
• Sample capacity: 4 segments useable for sample racks or well plates with SBS footprint format; plus a capacity of 12 x 22.5 mm O.D. vials in the carousel
• Included sample racks: (3) x Sample Racks 54 pos.x12 mm OD vials (≤ 1.5 mL)
• Barcode reading: empty segment detection, rack/well plate verification, inventory management

Vanquish Column Compartment H:

• Operating Principle Column Chamber: still air and forced air
• Temperature Range: 5–120 DegC in 0.1 DegC increments (18K below ambient)
• Temperature Stability: ±0.05K
• Temperature Accuracy: ±0.5K (up to 80 DegC)
• Heating Performance: <15 min, 20–50 DegC, ±1K Typical: 5 min, 25–40 DegC, ±1K
• Cooling Performance: <15 min, 50–20 DegC, ±1K
• Capacity: 2 column slots

Customization/Options: N/A

Testing performed by our in-house service team:

The Vanquish Flex Quaternary UHPLC System passed all tests and was found to be fully functional:

Vanquish Quaternary Pump F:

• We performed the initialization test.
• We conducted the self-test.
• We verified the software communication test.
• We performed the gradient proportioning valve test.
• We completed the seal-washing pump test.
• We ran the pressure limiter test.
• We tested the flow rate accuracy.
• We ran a solvent delivery stability.

Vanquish Split Sampler FT:

• We conducted the initialization test.
• We checked the software communication.
• We tested the sample cooler set temperature accuracy.
• We performed the needle wash test.
• We carried out the reproducibility test.

Vanquish Column Compartment H:

• We conducted the initialization test.
• We verified the software communication.
• We conducted the heating and cooling test.
• We checked the leak sensor.

Vanquish Variable Wavelength Detector F:

• We performed the initialization test.
• We verified the software communication.
• We checked the leak sensor.
• We conducted test injections of the test solution and verified the peaks.

Vanquish Fluorescence Detector F:

• We performed the initialization test.
• We verified the software communication.
• We checked the leak sensor.
• We conducted test injections of the test solution and verified the peaks.

Instrument Overview:

• Vanquish Flex Quaternary UHPLC System with Variable Wavelength and Fluorescence Detectors, configured for high‑resolution liquid chromatography.
• Quaternary pump provides four‑solvent mixing with precise gradient formation, supporting complex separations and method flexibility.
• Integrated static mixer (350 µL) minimizes dispersion and improves gradient stability, delivering sharper peaks and better reproducibility.
• Split‑loop autosampler enables accurate, low‑carryover injections with high precision and supports small‑volume/vial sample handling.
• Thermostatted column compartment offers tight temperature control with enhanced stability, improving retention time consistency and resolution.
• Dual detection combines VWD for quantitative absorbance measurements and FLD for highly sensitive, selective fluorescence analysis.

Instrument Application:

• Pharmaceutical assay and impurity profiling
• Trace detection of fluorescent biomarkers
• Bioanalytical quantification of proteins and peptides
• Stability testing and degradation studies
• Multi‑component mixture separation and identification

The Key Features:

• Quaternary 4‑solvent gradient control
• Low‑dispersion static mixer (350 µL)
• High‑precision split‑loop autosampler
• Tight column temperature regulation
• Dual detection: VWD + fluorescence
• Modular, UHPLC‑ready platform

Additional technical info:

• Manual: Thermo Vanquish UHPLC Manual
• Manual: Vanquish Split Sampler FT Manual
• Manual: Vanquish Quaternary Pump F Manual
• Manual: Vanquish Column Compartment H Manual
• Manual: Vanquish Variable Wavelength Detector Manual
• Manual: Vanquish Fluorescence Detector Manual

Video Overview:

About us

How we pack

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Why do so many customers choose to order from us?

• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see the questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• An extended warranty may be available
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

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