BD FACSymphony A5 Flow Cytometer (5)Lasers/(29)Colors/(31)Detectors w/ HTS Sampler & Supply System Unit 2

For Sale BD FACSymphony A5 Flow Cytometer (5)Lasers/(29)Colors/(31)Detectors w/ HTS Sampler & Supply System 

• Fully Functional
• Serviced & in Stock
• Expert sales consultation
• Made in 2022
•  Includes remote installation and instrument training session
•  Includes subscription to De Novo FCS Express 7 Analysis software
•  WARRANTY

Optional Services

•  Optional on-site setup and training are available for customers within 2 hours of Boston, MA
•  Optional remote installation support and training are available for non-local customers 

Service Highlights

•  Calibration beads test results match expected values
•  Laser power output confirmed stable over time
•  Instrument stress testing performed

Configuration Highlights

•  Includes 5 Laser, 96- & 384-well plate sampler
•  Includes a complementary subscription to De Novo FCS Express 7 Analysis software

Here is what you will receive:

• BD FACSymphony™ A5 Flow Cytometer Special Order Research Product
• BD High-Throughput Sampler (HTS)
• BD FACSFlow™ Supply System
• Fluidics Harness and sensors
• Computer with FACSDiva 9.3 Software and Dongle
• Monitors (Qty:2), Mouse & Keyboard
• BD Tables for the Cytometer and the PC
• Complimentary De Novo FCS Express analysis software (1-Year Subscription) RUO version
• Communication and power cables

When you purchase a flow cytometer from Boston Industries, you also receive a complimentary 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

• User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
• Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
• Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
• High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
• Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
• With FCS Express 7, you will have the latest Research Use analysis software.

BD FACSymphony A5 Flow Cytometer Configuration:

Optics:

• Laser Color Allocation (Laser/Channel Count): 6-Blue, 3-Red, 7-Violet, 5-Yellow/Green, 8-UV
• (5) Lasers: Blue 488 nm (200mW) / Red 628 nm (200mW) / Violet 405 nm (200mW) / UV 355 nm (60mW) / Yellow-Green 552 nm (200mW)
• (29) Fluorescence Colors simultaneously
• (31) Detector Channels (Manufacturer can upgrade the system up to 50 parameters)
• Forward scatter detector: Photodiode with a 488/10 BP filter for the 488-nm laser
• Side scatter detector: PMT with a 488/10 BP filter for the 488-nm laser
• 200mW 488 nm Blue Laser: 780/60, 750/30, 710/50, 670/30, 610/20, 515/20
• 200mW 405 nm Violet Laser: 780/60, 740/35, 710/50, 670/30, 610/20, 586/15, 470/15
• 140mW 640 nm Red Laser: 780/60, 710/50, 670/30
• 109mW 355 nm UV Laser: 820/60, 740/35, 670/30, 610/20, 586/15, 515/30, 450/50, 379/28
• 150mW 561 nm Yellow-Green Laser: 780/60, 710/50, 670/30, 610/20, 586/15

Performance:

• Fluorescence sensitivity: may vary depending on the combination of laser and filter selections
• Fluorescence resolution: coefficient of variation (CV) - PI: Area, <3.0%, full G⁰ /G¹ peak for PI-stained CEN
• Fluorescence linearity: doublet/singlet ratio: PI-stained CEN: 1.95–2.05 (488-nm laser)
• FSS sensitivity: sensitivity enables the separation of fixed platelets from noise
• FSS resolution: scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes
• SS resolution: scatter performance enables separation of 0.5-um beads from noise
• Acquisition rate: 40,000 events/second with beads

Fluidics:

• Sample flow rate modes: RUN, STANDBY, and PRIME.
• Continuously adjustable flow rate, plus three preset flow rates: LO: 12 ul/min; MED: 35 ul/min; HI: 60 ul/min

High Throughput Sampler:

• Automates and accelerates sample acquisition
• Compatible with 96- and 384-well plates
• <0.5% sample carryover in high-throughput mode, <0.75% in standard mode

FACSFlow Supply System: increases capacity and ease of use while maintaining a stable fluidics pressure.

Customization/Options: 

• Customization of detector configuration (Please inquire for more information)

Testing performed by our in-house service team:

The BD FACSymphony A5 Flow Cytometer passed all tests and was found to be fully functional:

• We verified the connection to the software to ensure proper communication and system control.
• We performed an extensive cleaning of the fluidic system to remove any residual contaminants.
• We conducted fluidics priming and sequencing to confirm smooth and consistent sample flow.
• We completed multiple cleaning cycles, including long clean and flow cell clean procedures.
• We ensured full functionality of the HTS (High-Throughput Screening) module.
• We performed an optical alignment of the laser and detector positioning.
• We verified the sheath and sample pressure values to ensure system stability and precision.
• We tested the calibration of each flow rate setting for consistent performance across all modes.
• We executed homing, priming, and cleaning procedures to confirm operational readiness.
• We conducted a run with Single-Peak Beads to validate optical and fluidic performance.
• We ran the CS&T Baseline and Performance tests with good CVs.

Instrument Overview:

• BD FACSymphony A5 Flow Cytometer is a high-parameter system for advanced multiparametric cell analysis.
• Five-laser optical configuration supports up to 29 colors and 31 detectors for expanded panel design.
• BD FACSymphony A5 Flow Cytometer provides high-sensitivity detection with optimized optics and fluidics.
• High-power laser configuration supports efficient excitation of dim fluorochromes and improved resolution of low-expression and rare cell populations
• Integrated HTS sampler enables automated acquisition from multiwell plates.
• Supply system supports stable operation during extended, high-throughput runs.
• BD FACSymphony A5 Flow Cytometer is designed for reliable performance in demanding laboratory environments.

Instrument Application:

• High-dimensional immunophenotyping of complex cell populations
• Multiparametric analysis using large fluorescence panels
• High-throughput sample acquisition from multiwell plates
• Deep immune profiling with large multicolor panels
• Rare cell population identification and characterization
• High-throughput screening with plate-based sampling
• Detailed analysis of cell activation and signaling states
• Phenotypic comparison across large sample sets

The Key Features:

• Five-laser optical configuration
• Supports up to 29 colors (Manufacturer can upgrade the system up to 50 parameters)
• Total of 31 detection channels
• High-sensitivity fluorescence detection
• HTS sampler for plate-based loading
• Integrated supply system for long runs

 De Novo FCS Express Software Features

• Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
• Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
• Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
• Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
• Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
• Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
• Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
• Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

 Benefits of the Complimentary DeNovo FCS Express Software

• Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
• User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
• Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
• Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
• Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
• Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
• Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
• Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

Additional technical info:

• Analysis Software: FCS Express Analysis Software
• Manual: BD FACSymphony A5 Flow Cytometer Manual
• BD FACSymphony A5 Flow Cytometer Baseline Report
• BD FACSymphony A5 Flow Cytometer Performance Report
• BD FACSymphony A5 Flow Cytometer Laser Configuration

Note: Subscription is for the Research Use Only (RUO) version. 
 

Video Overview:

About us

How we pack

 VIEW SOME OF THE CUSTOMERS WE HELPED HERE 

WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER FLOW CYTOMETERS FROM US?

  They want to ensure the cytometer undergoes full fluidics system decontamination, flushing, and inspection to protect the valves and flow sensors from damage.

  They want to ensure the cytometer has no residual sheath salt buildup in the flow cell, which could skew scatter data and, and if improperly cleaned, can damage the flow cell.

  They want to confirm that all detectors, including forward scatter (FSC) and side scatter (SSC) detectors, can be aligned and calibrated for particle sizing and granularity resolution.

  They want assurance that baseline performance has been verified using standardized particles, with data or log files available for review.

  They want to confirm that electronic noise and EMI levels at the detector/amplifier stage remain within tolerance and will not distort signal fidelity.

  They want to ensure that no fiber-optic cables are missing, misrouted, or defective, as this can lead to diminished fluorophore excitation or signal dropout.

  They want to confirm that no power degradation or misalignment in laser paths is detected through precise mW-level measurement at the final optics output.

  They want to ensure that optical filters and dichroic mirrors have been inspected for delamination, spectral shift, or contamination that could compromise signal specificity.

  They want to ensure the sample flow rate is linear and stable across a full range of flow settings, verified with volumetric calibration and pressure profiling.

  They want to ensure the system passes automated QC and performance tracking with reproducible CVs for bead standards across multiple channels.

  They want to ensure the fluidics pump assembly does not exhibit pulsation, drift, or backpressure due to check valve wear or micro-leaks.

  They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

• We have experience servicing and repairing these instruments.
  
• This instrument goes through all the described service steps before shipping. see questions section below
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

*LO* Long Crate
800#

COMMON QUESTIONS AND ANSWERS BELOW