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Sartorius Stedim Biostat B-DCU 4-Fold Cell Culture Bioreactor Univessel System
We apologize this Instrument is currently | |
Please submit a request, we will notify you if another one comes in stock. Thank you! | |
 | Approved Vendor to many US, Canada, and EU Universities |
| US & International Shipping |
- Stock: SOLD
- Brand: Sartorius Stedim
- Product Code: 61377
- Condition: Pre-Owned
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For Sale Sartorius Stedim Biostat B-DCU 4-Fold Cell Culture Bioreactor Univessel System
This item has been recently sold. Please view the other available instruments on your right.
If you do not see what you need, let us know via the “Request Quote” form; we often replenish sold-out items.
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- WARRANTY
- Includes remote instrument setup and training session
Here is what you will receive:
- (1x) BioPat DCU Tower
- (4x) Biostat B-DCU Cell Culture bioreactor stations
- (4x) 5 Liter Vessel / Single-walled UniVessel Autoclavable Glass
- (4x) Minebea Intec MW1P1E-60ED-I-
V1 Mildrics 1 Vessel Scales 60 kg - (4x) 435W Heating blanket
- (4x) BioPAT Trace Dual Channel Online Analysis System p/n: BPT001
- (4x) BioPAT Trace Dialysis probe length 332 mm LUER p/n: BPT0087
- (1x) Frigomix Recirculating Chiller p/n: FX-1000
- (4x) Temp sensor
- (4x) Optical Dissolved Oxygen sensor
- (4x) pH sensor
Each station includes:
- Power cord
- Connection cable to BioPat DCU Tower
- Agitation motor w/ attached cable
- pH extension cable
- O2 extension cable
- Temp sensor w/ cable
Biostat B-DCU Bioreactor Station Configuration Same for 4 Towers:
Gas flow supply connections:
| Gas flow control | 4 TMFCs (Thermal Mass Flow Controller), Precision: ±0.3% full scale; ± 0.5% current value; 4 Gas switching valves sparger to overlay |
| Gas outlets | Additive flow 4-gas (Air, O2, N2, CO2) aeration module with sparger and headspace outlet Equipped with four gas inputs (Air, O₂, N₂, and CO₂), the Advanced Additive Flow Module utilizes 4 Thermal Mass Flow Controllers and 4 gas switching valves to deliver precise gas flow to sparger and overlay ports, enabling advanced cascade control.
|
| Gas-in: | Air, O2, N2, CO2; Maximum 1.5 barg (22 psi) |
| Gas-out: | - Air: 0-500ccm
|
Controller peristaltic pumps:
| Built-in Pumps: | 5 Watson Marlow 114, fixed speed (on|off controlled) pumps with rotary switch |
| Pump Designations & Rotation Range: | Base, Acid (5 rpm); Subs-A, Subs-B, Subs-C (44 rpm) |
Univessel Glass (SW):
| Working volume: | 5 L |
| Mixing: | 2x3-blade segment impellers |
| Aeration: | L-sparger |
Process Control:
| Sensors | Measurement Range/Display Resolution |
| Temperature: | Pt100/0– 150°C (temperature control 0–80°C)/0.1°C |
| Dissolved oxygen, reusable: | optical/0– 100%/0.1% |
| pH, reusable: | combined measuring electrode/2– 12 pH/0.01 pH |
| Balance culture vessel: | 60 kg max./10 g (0.35 oz) |
| Biosensors: | BioPAT Trace Dialysis Probes for online monitoring of up to two parameters, including glucose and lactate or small-molecule alcohols, such as methanol and ethanol. |
Motor drive:
| Drive unit: | 200 W motor for 1L–10L Vessel |
| Stirrer shaft coupling: | Direct Coupling |
| Agitation range with direct drive: | 20-1500 RPM |
Temperature Control Module:
| Temperature control for Univessel Glass Single-wall Culture Vessels | External heating blanket and cooling finger |
| Temperature control range: | 8 DegC above cooling-water inlet temperature up to 60 DegC |
| Heating 435W blanket capacities for 5L vessel: | 400W
|
| Frigomix temperature control range: | -10 up to 40 DegC |
| Cooling capacity (at +30 DegC): | 1000W |
| Internal fluid tank: | 4.5 L |
| Pump flow rate: | 12 L/min (2.5 bar) |
Controller digital connections/inputs (Click here to expand this section)
| Port Label | Typical Accessory / Sensor | Purpose / Use |
|---|---|---|
| Temp | Pt-100 Temperature Sensor (M12 plug) | Measures culture vessel temperature. |
| PH-A/Redox-A | pH sensor A or Redox sensor A (VP8 plug) | Monitors pH and redox potential in the culture medium. |
| PO2-A | DO (Dissolved Oxygen) Sensor A (VP8 plug) | Measures dissolved oxygen in the medium. |
| Press | Pressure Sensor (M12 plug) | Monitors headspace or vessel pressure. |
| Ext.Sig-A/B | External Signal Input A/B (M12 plug) | Allows integration of analog signal inputs (0–10 V / 4–20 mA). |
| Ext.Sig-C/D | External Signal Input C/D (M12 plug) | Additional analog signal inputs for extended control or monitoring. |
| Ext.Sig-E/F | External Signal Input E/F (M12 plug) | Analog signal inputs; often used for weight balances or external sensors. |
| Heating blanket | Heating Jacket (Amphenol plug) | Heats single-walled culture vessels (up to 780W). |
| Exhaust Cooler (return) | Cooling Loop Return (Serto gland d 8 mm) | Returns exhaust cooling water from condenser. |
| Exhaust Cooler (inlet) | Cooling Loop Inlet (Serto gland d 8 mm) | Supplies cooling water to exhaust condenser. |
| Thermostat (return) | Thermostat Water Return (Serto gland d 10 mm) | Returns water from the vessel jacket or cooling blanket. |
| Thermostat (inlet) | Thermostat Water Inlet (Serto gland d 10 mm) | Supplies cooling/heating water to the vessel. |
| Ethernet, DCU-Tower | Ethernet Control Link (M12 plug) | Connects control tower to DCU system. |
| Fieldbus | Fieldbus Communication Port (M12 plug) | Enables communication via protocols like Modbus or OPC. |
| Serial-A | Balance A (RS-232/M12 plug) | Connects to Sartorius balance or similar. |
| Serial-B | Balance B (RS-232/M12 plug) | Additional balance input. |
| Serial-C | Balance C (RS-232/M12 plug) | Additional balance input. |
| Serial-D | Balance D (RS-232/M12 plug) | Additional balance input. |
| Serial-E | BioPAT® Viamass (RS-485/M12 plug) | Viable biomass sensor for capacitance-based measurement. |
| Serial-F | UniVessel® SU (optical sensors, RS-485/M12 plug) | Single-use vessel integration with SU-compatible optical sensors. |
| Pump-A | External Pump A (M12 plug) | Drives substrate addition or liquid transfer. |
| Pump-B | External Pump B (M12 plug) | Additional external pump support. |
| Pump-C | External Pump C (M12 plug) | Additional external pump support. |
| Pump-D | External Pump D (M12 plug) | Additional external pump support. |
| Ext. Drive | External Stirrer Motor Control (M12 plug) | Connects and controls magnetic stirrer or top-drive motors. |
| Power Out | Power Output (M12 plug) | Supplies power to external devices (e.g., vessel light, sensors). |
| PA | Potential Equalization | Safety grounding point to avoid electric discharge. |
| Mains | Main Power Supply | Primary power input for Biostat® B-DCU system. |
| Air | Compressed Air Supply | Base gas for aeration, cascade control. |
| O2 | Oxygen Supply | Used for DO control via enrichment in sparger or overlay. |
| N2 | Nitrogen Supply | Used for inert gassing or stripping oxygen. |
| CO2 | Carbon Dioxide Supply | Used for pH control in mammalian cell cultures. |
| Cooling Water (inlet) | Cooling Medium Inlet (nozzle d10 mm) | Inflows cooling water to temperature control jacket or cooling finger. |
| Cooling Water (return) | Cooling Medium Return (nozzle d10 mm) | Outflow of warmed water back to chiller. |
| Stirrer connection cable | Stirrer Motor Signal Cable | Transfers drive signal to the motor on the culture vessel. |
(* Depending on the configuration of the device, the port may be present but not configured for operation. Configuration changes must be made by the manufacturer.)
Controller Weight/Dimensions/Power Requirements
| Power requirements | 120 V (±10%),60 Hz, max. power consumption 12 A |
| Dimensions (W x D x H) | 360×768×432 cm (14.2×30.2×17.0in.) |
| Net weight: | 45 kg (99 lbs) |
Customization/Options:
- Exhaust coolers (Please inquire for available inventory)
- Additional Vessel Scales
- Other vessel sizes may be available
- Other customizations may be available
Testing performed by our in-house service team: (Final testing will be adjusted to the final configuration)
The Biostat B-DCU System passed all tests and was found to be fully functional:
- We tested the power supply to the system.
- We tested the operation of the touchscreen display.
- We tested the communication between the control unit and the bioreactor stations.
- We checked the operation of all peristaltic pumps.
- We verified the functionality of the agitation motors.
- We verified the communication and accuracy of the temperature probes.
- We tested the communication and responsiveness of the dissolved oxygen (DO) sensors.
- We confirmed the proper operation of the heating blankets.
- We verified the accuracy of the scales.
- We tested the functionality of the gas mixing components.
- We confirmed the functionality of the coolant distribution components.
Instrument Overview:
- Includes 4 cell culture stations with 5L single-walled UniVessel glass for small to mid-scale bioprocessing.
- Each vessel integrates Minebea Intec vessel scales, heating blanket, temp, pH, and dissolved oxygen sensors.
- BioPAT Trace system enables dual-channel online analysis with dialysis probes for nutrient monitoring.
- Frigomix recirculating chiller supports temperature control for consistent culture conditions.
- Modular tower design links and controls all bioreactor stations for coordinated operation.
- Suitable for parallel processing with uniform measurement and control across all vessels.
Instrument Application:
- Parallel cell line development
- Media optimization studies
- Small-scale upstream process development
- Batch and fed-batch cultivation
- Scale-down model validation
The Key Features:
- 4-fold parallel bioreactor configuration
- Modular, compact benchtop design
- Independent vessel parameter control
- Integrated pumps and gas flow system
- Precise pH, DO, temp, and speed control
Additional technical info:
- Manual: Biostat B-DCU Fermenter System Manual
- Manual: BioPAT Trace Dual Channel Online Analysis System Manual
Video Overview:
About us
How we pack
Why do so many customers choose to order from us?
- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
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COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Biostat B-DCU System?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards..(Final testing will be adjusted to the final configuration)
- We tested the power supply to the system.
- We tested the operation of the touchscreen display.
- We tested the communication between the control unit and the bioreactor stations.
- We checked the operation of all peristaltic pumps.
- We verified the functionality of the agitation motors.
- We verified the communication and accuracy of the temperature probes.
- We tested the communication and responsiveness of the dissolved oxygen (DO) sensors.
- We confirmed the proper operation of the heating blankets.
- We verified the accuracy of the scales.
- We tested the functionality of the gas mixing components.
- We confirmed the functionality of the coolant distribution components.
What supplies do I need?
You will need the consumables starting out:
Can I view the Biostat B-DCU System in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Biostat B-DCU System used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We tested the power supply to the system.
- We tested the operation of the touchscreen display.
- We tested the communication between the control unit and the bioreactor stations.
- We checked the operation of all peristaltic pumps.
- We verified the functionality of the agitation motors.
- We verified the communication and accuracy of the temperature probes.
- We tested the communication and responsiveness of the dissolved oxygen (DO) sensors.
- We confirmed the proper operation of the heating blankets.
- We verified the accuracy of the scales.
- We tested the functionality of the gas mixing components.
- We confirmed the functionality of the coolant distribution components.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a Biostat B-DCU System on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Biostat B-DCU System Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
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