- Microbial fermentation process development
![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | International Shipping |
![]() | Customer References Available |
![]() | Training and Installation Options Available |
- Stock: In Stock
- Brand: Sartorius
- Product Code: 63569
- Condition: Pre-Owned
For Sale Sartorius Biostat B Twin Bioreactor for Microbial Cultures with 1 Liter Jacketed Autoclavable Vessels
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
- Includes remote instrument setup and training session
We are a trusted equipment source to many Fortune 500 companies, small/midsize companies, and universities
Below is how we ensure a smooth transaction:
- We price bioreactors very competitively, reflecting the extensive technical hours each one receives
- Our team is versed in these instruments and is happy to jump on a call (or email) to answer your questions
- We accurately verify what we sell, providing operational videos and detailed testing information
- Most bioreactors receive between 10 and 60 hours of technician time to ensure they operate properly
- We are well-versed in global logistics, having safely shipped various instruments to over 40 countries
- We offer professional post-sale technical support, resulting in virtually no returns in 2024 & 2025.
Here is what you will receive:
(1x) Sartorius Biostat B twin control tower for 2 vessels
(2x) 1-Liter autoclavable Double-wall (Jacketed) Glass Vessel (Other vessels and sizes are available.)
- (2x) Direct drive agitation motors with attached cables
- (2x) pH sensors with cable
- (2x) DO sensors with cable
- (2x) Temp sensor with cable
- (2x) Feed/Supply Weight Scales
- (2x) Vessels Scales
- (1x) Power cord
Kindly take note: Tubing and other consumables are not included. Inquire with us about any accessories or components you would like to have added.
We have other Biostat B controller configurations that may better suit your application.
Sartorius Biostat B Bioreactor Controller Configuration: same for both sides
Gas flow supply connections
| Gas flow control | 2 TMFCs (Thermal Mass Flow Controller), Precision: ±1% full scale; 2 Flow meters, Precision: ±5% full scale |
| Gas mixing option | 2-Gas mixing (Air, O2) with sparger outlet |
| (Gas-in) Supply ports | Air, O2; Maximum 1.5 barg |
| (Gas-out) Sparge connection | 02: up to 3 lpm (MFC) Air: up to 3 lpm (MFC) |
Controller peristaltic pumps
| Built-in Pumps: | (2) x 4 Watson Marlow 114, fixed speed (on|off controlled) pumps for pH control (acid | base) and substrate |
| Rotation Range: | SUBS A, SUBS B, ACID (5 rpm); BASE (44 rpm) |
Univessel Culture Vessel: Autoclavable Univessel Glass Double-wall (Jacketed) Culture Vessels (Other vessels and sizes are available.)
| Working volume range: | 0.35 to 1 L (Total volume 1.6 L) |
| Mixing: | 1 x 3-blade segment impeller |
| Aeration: | Ring sparger |
Motor drive
| Drive unit: | 200 W motor for 1L–10L Vessel |
| Stirrer shaft coupling: | Direct Coupling |
| Agitation ran ge with direct drive: | 20-2000 RPM |
Temperature Control Module
| Temperature control for Univessel Glass DW (Jacketed) Vessels: | Open thermostat system with recirculation pump and automatic cooling water valve |
| Temperature control range: | 8 DegC above the cooling-water inlet temperature of up to 80 DegC |
| Heating capacity: | 600 W |
Controller digital connections/inputs (Click here to expand this section)
| Port Label | Typical Accessory / Sensor |
|---|---|
| Serial-A Scales connection(FWEIGHT), RS-232, M12 plug | Balance/culture vessel weight sensor (e.g., 60 kg/300 kg max)* |
| Serial-B Scales connection (FWEIGHT /VWEIGHT), RS-232, M12 plug. | Substrate weight or vessel load cell for gravimetric feed control* |
| Serial-C RS-232, M12 plug. | Biostat RM Rocker connection |
| Temp - Temperature sensor, M12 plug | Pt100 temperature sensor (0–150 °C, ±0.1 °C accuracy) |
| Thermostat (x2) Heating/cooling control return and inlet | |
| pH/Redox pH sensor or Redox sensor input, VP8 plug | Combined pH/Redox sensor (e.g., Mettler Toledo InPro series) |
| pO₂ pO2 sensor input, VP8 plug | Optical DO sensor (e.g., VisiFerm DO, Mettler InPro 6800) |
| External Signal A/B input, M12 plug | Control signals fro process monitoring or control |
| External Signal C/D, M12 plug | Digital trigger signals (start/stop, alarms, status outputs) |
| Exhaust (x2) Exhaust cooler control inlet/return | Cooling water loop for glass/single-use exhaust condenser |
| Sparger Aeration control | Sparger line for Air, O₂ (with Flow meter or MFC control) |
| Pump-B / Pump-C External peri-pumps for acid/base/antifoam addition, M12 plug | Watson-Marlow 120 series pumps (speed-controlled, external) |
| Stirrer (x2) Stirring motor drive and encoder feedback | Direct drive or magnetic coupling motors (e.g., Sartorius 200 W) |
(* Depending on the configuration of the device, the port may be present but not configured for operation. Configuration changes must be made by the manufacturer.)
Controller Weight/Dimensions/Power Requirements
| Power requirements | 120 V (±10%),60 Hz, max. power consumption 12 A |
| Dimensions (W x D x H) | 35×82.2×43 cm (14 ×32 × 17 in) |
| Net weight: | 55 kg (121 lbs) |
Customization/Options: (Please inquire with us for available inventory)
- Exhaust condensers
- Alternative glass vessel sizes (Up to 10L)
- Heating/cooling circulator
- Heated exhaust filters
- External chiller
- Watson-Marlow 120 external feed pump
- Alternative impeller types
Please note that, depending on the requested options, we may recommend a different bioreactor model that better suits your application.
Testing performed by our in-house service team:
The Sartorius Biostat B Twin Bioreactor Controller passed all tests and was found to be fully functional:
- We verified the system startup and ensured the successful initialization of all core modules.
- We tested the touchscreen display's responsiveness and accuracy.
- We verified the operation of all peristaltic pumps for acid/base, and substrate feed.
- We tested the temperature and agitation control systems for proper regulation and stability.
- We verified the accuracy of the vessel and feed scales and confirmed stable weight measurements.
- We verified the pH probes using standard buffer solutions.
- We confirmed proper DO probe response through calibration and functional testing.
- We confirmed proper temperature control and verified effective heating through the jacketed vessel system.
- We evaluated the gas flow control and sparging functions using NIST-calibrated mass flow meters.
- We confirmed proper operation of the cooling water distribution system for effective heat removal.
- We performed a rinse cycle and verified that all fluid lines were free from blockages and contamination.
Instrument Overview:
- Sartorius Biostat B Twin bioreactor supports controlled microbial cultivation in 1 L jacketed autoclavable glass vessels.
- Dual-vessel configuration enables parallel fermentation runs with independent control of key process parameters.
- Integrated control of temperature, pH, dissolved oxygen, agitation speed, and aeration.
- Precision gas delivery of air and O₂ through spargers using thermal mass flow controllers and flow meters.
- Direct-drive agitation with a 200 W motor supports speeds up to 2000 rpm for efficient mixing and oxygen transfer.
- Multiple pumps enable precise acid, base, and substrate addition to maintain stable pH and controlled nutrient supply during fermentation.
- Includes feed weight scales and vessel scales for monitoring media addition and culture mass during operation.
- Jacketed vessels enable precise temperature control using an external heating/cooling circulator. (Not included)
Instrument Application:
- Media and nutrient optimization studies
- Strain screening and characterization
- Batch and fed-batch fermentation experiments
- Small-scale bioprocess optimization
- Aeration and agitation strategy testing
The Key Features:
- Two 1L double-wall (jacketed), autoclavable glass vessels
- Precision gas mixing control for Air and O₂
- Direct-drive agitation system up to 2000 rpm
- Jacketed vessel design for efficient temperature control
- Integrated peristaltic pumps for acid and substrate feed
- Thermal mass flow controllers for accurate gas delivery
Additional technical info:
Video Overview:
About us

How we pack

VIEW SOME OF THE CUSTOMERS WE HELPED HERE
WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER BIOREACTORS FROM US?
They want to ensure that the bioreactor controller has been thoroughly tested and responds to commands.
They want to know whether the sensors have been tested against appropriate standards or calibrated using appropriate tools.
They want to ensure the paired drive is correctly matched to the required vessel size and type.
They want to ensure that the vessel’s drive RPMs are verified against the tachometer.
They want to ensure that the bioreactor controller can detect all attached sensors.
They want to ensure that all peristaltic pumps are tested through their maximum RPMs.
They want to ensure that the mass flows have been properly tested using a NIST traceable standard.
They want peace of mind knowing it will be safely and professionally packed for shipping.
They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*ME* Medium Crate
425#
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Sartorius Biostat B Twin Bioreactor Controller?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.
- We verified the system startup and ensured the successful initialization of all core modules.
- We tested the touchscreen display's responsiveness and accuracy.
- We verified the operation of all peristaltic pumps for acid/base, and substrate feed.
- We tested the temperature and agitation control systems for proper regulation and stability.
- We verified the accuracy of the vessel and feed scales and confirmed stable weight measurements.
- We verified the pH probes using standard buffer solutions.
- We confirmed proper DO probe response through calibration and functional testing.
- We confirmed proper temperature control and verified effective heating through the jacketed vessel system.
- We evaluated the gas flow control and sparging functions using NIST-calibrated mass flow meters.
- We confirmed proper operation of the cooling water distribution system for effective heat removal.
- We performed a rinse cycle and verified that all fluid lines were free from blockages and contamination.
What supplies do I need?
You will need the consumables starting out:
Can I view the Sartorius Biostat B Twin Bioreactor Controller in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Sartorius Biostat B Twin Bioreactor Controller used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We verified the system startup and ensured the successful initialization of all core modules.
- We tested the touchscreen display's responsiveness and accuracy.
- We verified the operation of all peristaltic pumps for acid/base, and substrate feed.
- We tested the temperature and agitation control systems for proper regulation and stability.
- We verified the accuracy of the vessel and feed scales and confirmed stable weight measurements.
- We verified the pH probes using standard buffer solutions.
- We confirmed proper DO probe response through calibration and functional testing.
- We confirmed proper temperature control and verified effective heating through the jacketed vessel system.
- We evaluated the gas flow control and sparging functions using NIST-calibrated mass flow meters.
- We confirmed proper operation of the cooling water distribution system for effective heat removal.
- We performed a rinse cycle and verified that all fluid lines were free from blockages and contamination.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a Sartorius Biostat B Twin Bioreactor Controller on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Sartorius Biostat B Twin Bioreactor Controller Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

