





























































































![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | International Shipping |
![]() | Customer References Available |
![]() | Training and Installation Options Available |
- Stock: In Stock
- Brand: Sartorius
- Product Code: 66283
- Condition: Pre-Owned
For Sale Sartorius Biostat B-DCU 6-Fold Cell Culture Univessel Bioreactor System (Autoclavable or Single Use configurations available)
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- WARRANTY
- Optional remote instrument setup and training are available
We are a trusted equipment source to many Fortune 500 companies, small/midsize companies, and universities
Below is how we ensure a smooth transaction:
- We price bioreactors very competitively, reflecting the extensive technical hours each one receives
- Our team is versed in these instruments and is happy to jump on a call (or email) to answer your questions
- We accurately verify what we sell, providing operational videos and detailed testing information
- Most bioreactors receive between 10 and 60 hours of technician time to ensure they operate properly
- We are well-versed in global logistics, having safely shipped various instruments to over 40 countries
- We offer professional post-sale technical guidance and optional instrument training.
Here is what you will receive: (This is an example configuration, please inquire to build the best configuration(s) for your application)
- (1x) BioPat DCU Tower
- (6x) Biostat B-DCU Cell Culture bioreactor stations
- (6x) Agitation motor with/ attached cable
- (6x) 2L Univessel Glass Single-wall Culture Vessels – Also available as single use configuration
- (6x) 220W Heating blanket (we also have cooling blankets avaialble)
- (6x) Temperature sensor and cable
- (6x) pH sensor and cable
- (6x) Dissolved oxygen sensor and cable
- (12x) Feed/Supply Weight or Vessels Scales p/n: EVO1S1-WV4 (4kg max.)
Please note: Consumables are not included. Inquire with us about any accessories or components you would like to have added.
Pre-Configured Process Modes: the system includes three pre-built configurations optimized for common bioprocess applications.
- Mammalian cell culture: CO₂ overlay for pH control (Air + CO₂ mixing – typical for mammalian culture processes)
- Microbial fermentation: O₂ enrichment overlay for dissolved oxygen control (Air + O₂ mixing – typical for high oxygen demand microbial processes)
- Fed-batch operation: Gravimetric substrate or feed dosing into the reactor vessel, supporting both mammalian and microbial cultivation processes
Vessel Compatibility: This system is configured to support both single-use and reusable vessel platforms, offering flexibility for process development environments.
- It is compatible with Univessel SU single-use stirred tank reactors for mammalian cell culture.
- Reusable glass vessels, including single-wall and jacketed configurations, for fermentation and advanced development applications.
Optional configurations available on this system:
Autoclavable Vessels (up to 10 Liters possible, depending on gassing strategy requirements, see mass flow specs below and discuss with our sales team)

Single-use Vessel configuration

Gravimetric Feed Dosing (Hanging Bag on Scale)

Biostat B-DCU System Configuration: Same for 6 Towers
Gas flow supply connections
| Gas flow control | 6 TMFCs (Thermal Mass Flow Controller), Precision: ±0.3% full scale; ± 0.5% current value |
| Gas outlets | Advanced Additive Flow 4-gas aeration module with sparger and overlay (headspace) outlets. The system features four gas inputs (Air, O₂, N₂, CO₂) and utilizes six TMFCs across six gas lines, including overlay lines for Air and CO₂. Depending on the chosen configuration, O₂ can also be routed to the overlay for headspace oxygen enrichment or additional dissolved oxygen control. This flexible architecture enables precise, independent gas delivery and advanced cascade control for optimized process performance.
|
| (Gas-in) Supply ports: | Air, O2, N2, CO2; Maximum 1.5 barg (22 psi) |
| Gas-out: (Be sure to discuss your gassing needs with our team) | Overlay: - Air: 0-300ccm - CO2: 0-150ccm Sparger: - Air: 0-3lpm |
Controller peristaltic pumps (Additional Pumps can be added upon request)
| Built-in Pumps: | 3 x Watson Marlow 114 fixed speed (on|off controlled) pumps with rotary switch |
- Scales provide real-time gravimetric monitoring of feed bottles, supply containers, or reactor vessels, supporting precise dosing and flexible mass-based process control (4 kg max.)
Univessel Glass (SW) Culture Vessel (As listed, other vessel options available)
| Working volume range: | 0.4 to 2 L (Total Volume: 3L) |
| Mixing: | (2) x 3-blade segment impeller |
| Aeration: | L-sparger |
Motor drive
| Drive unit: | 200 W motor for 1L–10L Vessel |
| Stirrer shaft coupling: | Direct Coupling |
| Agitation ran ge with direct drive: | 20-2000 RPM |
Temperature Control Module
| Temperature control for Univessel SU Single-use Vessels | Electrical heating blanket |
| Temperature control range: | DegC above cooling-water inlet temperature up to 60 DegC |
| Heating capacity: | 170W |
Controller digital connections/inputs (Click here to expand this section)
| Port Label | Typical Accessory / Sensor | Purpose / Use |
|---|---|---|
| Temp | Pt-100 Temperature Sensor (M12 plug) | Measures the culture vessel temperature. |
| Press | Pressure Sensor (M12 plug) | Monitors headspace or vessel pressure. |
| Ext.Sig-A/B | External Signal Input A/B (M12 plug) | Allows integration of analog signal inputs (0–10 V / 4–20 mA). |
| Ext.Sig-C/D | External Signal Input C/D (M12 plug) | Additional analog signal inputs for extended control or monitoring. |
| Ext.Sig-E/F | External Signal Input E/F (M12 plug) | Analog signal inputs are often used for weight balances or external sensors. |
| Heating blanket | Heating Jacket (Amphenol plug) | Heats single-use culture vessels. |
| Exhaust Cooler (return) | Cooling Loop Return (Serto gland d 8 mm) | Returns exhaust cooling water from the condenser. |
| Exhaust Cooler (inlet) | Cooling Loop Inlet (Serto gland d 8 mm) | Supplies cooling water to the exhaust condenser. |
| Thermostat (return) | Thermostat Water Return (Serto gland d 10 mm) | Returns water from the vessel blanket. |
| Thermostat (inlet) | Thermostat Water Inlet (Serto gland d 10 mm) | Supplies cooling/heating water to the vessel. |
| Ethernet, DCU-Tower | Ethernet Control Link (M12 plug) | Connects the control tower to the DCU system. |
| Fieldbus | Fieldbus Communication Port (M12 plug) | Enables communication via protocols like Modbus or OPC. |
| Serial-A | Balance A (RS-232/M12 plug) | Connects to Sartorius balance or similar. |
| Serial-B | Balance B (RS-232/M12 plug) | Additional balance input. |
| Serial-C | Balance C (RS-232/M12 plug) | Additional balance input. |
| Serial-D | Balance D (RS-232/M12 plug) | Additional balance input. |
| Serial-E | BioPAT® Viamass (RS-485/M12 plug) | Viable biomass sensor for capacitance-based measurement. |
| Serial-F | UniVessel® SU (optical sensors, RS-485/M12 plug) | Single-use vessel integration with SU-compatible optical sensors. |
| Pump-A | External Pump A (M12 plug) | Drives substrate addition or liquid transfer. |
| Pump-B | External Pump B (M12 plug) | Additional external pump support. |
| Pump-C | External Pump C (M12 plug) | Additional external pump support. |
| Pump-D | External Pump D (M12 plug) | Additional external pump support. |
| Ext. Drive | External Stirrer Motor Control (M12 plug) | Connects and controls magnetic stirrer or top-drive motors. |
| Power Out | Power Output (M12 plug) | Supplies power to external devices (e.g., vessel light, sensors). |
| PA | Potential Equalization | A safety grounding point to prevent an electric discharge. |
| Mains | Main Power Supply | Primary power input for the Biostat® B-DCU system. |
| Air | Compressed Air Supply | Base gas for aeration, cascade control. |
| O2 | Oxygen Supply | Used for DO control. |
| N2 | Nitrogen Supply | Used for inert gassing or stripping oxygen. |
| CO2 | Carbon Dioxide Supply | Used for pH control in mammalian cell cultures. |
| Cooling Water (inlet) | Cooling Medium Inlet (nozzle d10 mm) | Inflows of cooling water to the temperature control jacket or the cooling finger. |
| Cooling Water (return) | Cooling Medium Return (nozzle d10 mm) | Outflow of warmed water back to the chiller. |
| Stirrer connection cable | Stirrer Motor Signal Cable | Transfers drive signal to the motor on the culture vessel. |
(* Depending on the configuration of the device, the port may be present but not configured for operation. Configuration changes must be made by the manufacturer.)
Controller Weight/Dimensions/Power Requirements
| Power requirements | 120 V (±10%),60 Hz, max. power consumption 12 A |
| Dimensions (W x D x H) | 360×768×432 cm (14.2×30.2×17.0in.) |
| Net weight: | 45 kg (99 lbs) |
Please note that, depending on the requested options, we may recommend a different bioreactor model that better suits your application.
Customization/Options: (Available options include but are not limited to the following; please inquire for additional options)
- Additional temperature sensors
- pH sensors with cables
- DO (dissolved oxygen) sensors with cables
- Safety valve station/overpressure protection modules
- Alternative vessel scale configurations (higher capacity or precision options)
- Exhaust filter heaters (for condensation control)
- Off-gas / exhaust analyzers (O2 / CO2 monitoring modules)
- Motor adaptors for SU vessels
- Additional Pumps can be added
Testing performed by our in-house service team:
The Biostat B-DCU System passed all tests and was found to be fully functional:
- We verified stable power input and tested the power supply to the system.
- We tested the system startup and shutdown procedures and confirmed proper initialization of the BioPat DCU tower.
- We tested the operation of the touchscreen display and confirmed accurate responsiveness.
- We verified communication between the control unit and six bioreactor stations and confirmed stable connectivity.
- We checked the operation of peristaltic pumps and verified accurate flow control.
- We verified the functionality and speed control of agitation motors.
- We tested the communication and accuracy of the temperature probes and confirmed proper calibration response.
- We confirmed proper operation of the heating blankets.
- We tested the functionality of the gas-mixing components and verified proper gas-flow control using a NIST calibrated massflow.
- We verified the accuracy of the vessel and feed scales and confirmed that weight measurements were stable.
- We verified alarm functionality and confirmed proper operation of safety interlocks.
Instrument Overview:
- The Sartorius Biostat B-DCU system is designed for independent parallel cell culture and fermentation processes, supporting efficient bioprocess development.
- The platform consists of six bioreactor stations connected to a centralized BioPAT® DCU Tower, providing unified monitoring and control of all process parameters.
- Each station integrates agitation, gas mixing, and heating capabilities, ensuring stable and reproducible process conditions.
- Key parameters such as temperature, pH, and dissolved oxygen (DO) are precisely controlled to maintain optimal growth environments.
- The system’s compact footprint and modular architecture allow flexible configurations to accommodate different laboratory scales and workflows.
- Parallel operation enables high process reproducibility while minimizing cross-contamination risk between reactors.
Instrument Application:
- Mammalian cell culture development and optimization
- Microbial fermentation process development
- Parallel screening of cultivation conditions
- Media and feed strategy optimization
- Cell line and strain characterization
- Early-stage bioprocess development and scale-up studies
- Fed-batch and batch cultivation experiments
The Key Features:
- Six independent bioreactor stations for parallel bioprocess development.
- Centralized BioPAT DCU control tower for unified system management.
- Precise control of temperature, pH, dissolved oxygen, and agitation.
- Advanced 4-gas aeration module supporting Air, O₂, N₂, and CO₂.
- Flexible vessel compatibility with Univessel SU and reusable glass reactors.
- Gravimetric feed dosing for accurate fed-batch cultivation processes.
Additional technical info:
- Manual: Biostat B-DCU System Manual
Video Overview:
About us

How we pack

VIEW SOME OF THE CUSTOMERS WE HELPED HERE
WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER BIOREACTORS FROM US?
They want to ensure that the bioreactor controller has been thoroughly tested and responds to commands.
They want to know whether the sensors have been tested against appropriate standards or calibrated using appropriate tools.
They want to ensure the paired drive is correctly matched to the required vessel size and type.
They want to ensure that the vessel’s drive RPMs are verified against the tachometer.
They want to ensure that the bioreactor controller can detect all attached sensors.
They want to ensure that all peristaltic pumps are tested through their maximum RPMs.
They want to ensure that the mass flows have been properly tested using a NIST traceable standard.
They want peace of mind knowing it will be safely and professionally packed for shipping.
They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

- Warranty
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
900#
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Biostat B-DCU System?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.
- We verified stable power input and tested the power supply to the system.
- We tested the system startup and shutdown procedures and confirmed proper initialization of the BioPat DCU tower.
- We tested the operation of the touchscreen display and confirmed accurate responsiveness.
- We verified communication between the control unit and six bioreactor stations and confirmed stable connectivity.
- We checked the operation of peristaltic pumps and verified accurate flow control.
- We verified the functionality and speed control of agitation motors.
- We tested the communication and accuracy of the temperature probes and confirmed proper calibration response.
- We confirmed proper operation of the heating blankets.
- We tested the functionality of the gas mixing components and verified proper gas-flow control using a NIST calibrated massflow.
- We verified the accuracy of the vessel and feed scales and confirmed stable weight measurements.
- We verified alarm functionality and confirmed proper operation of safety interlocks.
What supplies do I need?
You will need the consumables starting out:
- Cell Culture Media & Buffers | Sartorius
- Univessel® SU - Stirred Tank Single-Use Bioreactor | Sartorius
Can I view the Biostat B-DCU System in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Biostat B-DCU System used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We verified stable power input and tested the power supply to the system.
- We tested the system startup and shutdown procedures and confirmed proper initialization of the BioPat DCU tower.
- We tested the operation of the touchscreen display and confirmed accurate responsiveness.
- We verified communication between the control unit and six bioreactor stations and confirmed stable connectivity.
- We checked the operation of peristaltic pumps and verified accurate flow control.
- We verified the functionality and speed control of agitation motors.
- We tested the communication and accuracy of the temperature probes and confirmed proper calibration response.
- We confirmed proper operation of the heating blankets.
- We tested the functionality of the gas mixing components and verified proper gas-flow control using a NIST calibrated massflow.
- We verified the accuracy of the vessel and feed scales and confirmed stable weight measurements.
- We verified alarm functionality and confirmed proper operation of safety interlocks.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a Biostat B-DCU System on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Biostat B-DCU System Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.















































