Nikon Eclipse Ti2-E Inverted Fluorescence Phase Contrast Microscope (New Filters) Unit 5

Nikon Eclipse Ti2-E Inverted Fluorescence Phase Contrast Microscope (New Filters) Unit 5

	Approved Vendor to many US, Canada, and EU Universities
	US & International Shipping
	Hundreds of Custom Microscopes Shipped
	Customer References Available
	Training and Installation Options Available

Stock: In Stock
Brand: Nikon
Product Code: 55915
Condition: Pre-Owned

$37,995.00

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For Sale Nikon Eclipse Ti2-E Inverted Fluorescence Phase Contrast Microscope Unit 5

Fully Functional
Serviced & in Stock
Customized to your requirements
Includes new fluorescence filters
Technical sales consultation available
Continental USA 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
Includes remote microscope training session and software overview
More info about our Microscopy Capabilities or view the Microscope Buying Guide

This is what you will receive before customizing this microscope:

Standard Nikon Eclipse Ti2-E Microscope Configuration:

Microscopy Techniques:

Brightfield with Phase Contrast studies and reflective Fluorescence Microscopy

Microscope Base:

Nikon Eclipse TI2-E
Nikon TI2-CTRE Control Box

Microscope Head:

TC-T-TS Binocular Head
CFI 10/22 Eyepiece (Qty. 2)

Fluorescence Light Path:

Liquid Light Guide
Collimating Adapter
Reflective Light Path

Fluorescence Illumination:

Lumencor Aura fluorescence source

Fluorescence filter cube turret:

Fluorescence Cube Motorized 6-Position Turret TI2-F-FLT-E

Fluorescence filters:

Three empty filter cubes
Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
*Other filters available - please inquire*

Brightfield Light Path:

Built-into the base

Brightfield Illumination:

Illumination Pillar TI2-D-PD
Halogen lamp w/ TI-PS100 power supply

Objective Turret:

Motorized 6-position objectives turret

Objectives:

4x/0.13 Plan Fluor PhL
10x/0.30 Plan Fluor Ph1
20x/0.75 Plan Apo VC
(This is a high quality Nikon APO CFI60 Objective; it was originally installed in a custom piece of equipment where the manufacturer did not want to show that they used Nikon objectives)
Other objectives available (APO, higher N.A, etc.)

Condenser:

Mechanical Condenser Turret w/ Ph inserts
LWD Manual Condenser Lens with 0.52 Lens

Stage:

TI2-S-SS-E Motorized XY Stage
Nikon TI2-CTRE Control Box
TI2-S-JS joystick

Photo/Port Splits:

Left - 100%
Right - 100%
Eyepieces - 100%
Eyepieces/Left - 20%/80%

Internal Magnification:

1x-1.5x

Camera:

8.3MP CMOS Camera (we have other CCD and Scmos cameras we can upgrade to)

Computer & Software:

Computer running Camera Imaging Software and MicroManager

Accessories:

Necessary power and communication cables

This configuration includes any 3 of these new filter sets.

New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)

Automated/motorized capabilities of this microscope:

Installed automation/motorized components:

Motorized XY Stage
Motorized EPI Fluorescence Path Shutter
Motorized Objective Nosepiece
Motorized Fluorescence Filter Turret
Motorized Port Splitting
Motorized Z-Axis

Benefits of motorized/Automated Z-Axis drive:

Automatic parfocality adjustment
The ability to recall previously saved focus levels
Automated management of Z positioning/ Fine-coarse control

This microscope's imaging capabilities:

Imaging software highlights:

The included camera software allows for live viewing and image/video capture with variable linear measurements such as line segment, radius values, and others.
The easy-to-use interface also allows for customization of color and resolution settings.
The Micro-Manager software enables control of motorized components.
Multi-slice (Z coordinate/Z Stacking)
*Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage

We can customize this microscope to fit your requirements:

Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)

Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality:

Microscope can be configured for magnification between 40x and 200x (with 10x Eyepieces)
We can add standard higher magnification Nikon microscope objectives
We can add high-end Nikon Apochromat objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

Additional filter cubes
Additional filter sets to match your fluorochromes.
Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:

We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

We can configure it to work with the sample medium you need:

Slide, Petri dish, microplate, etc.

Commonly Requested Customer Upgrades

High-End CCD, sCMOS Cameras
LED or Metal Halide Fluorescence Light Sources
Phase Contrast, Polarization, or the DIC configurations
Higher Magnification Objectives
Higher performance Fluor or APO objectives

We are excited to help build and configure the right microscope for you!

Nikon Eclipse Ti2-E Microscope Overview

(Final microscope capabilities will depend on the modifications you request)

This Nikon Eclipse Ti2-E Inverted Fluorescence Phase contrast Microscope is currently set up for Brightfield with Phase Contrast studies and reflective Fluorescence studies.
The camera is capable of detailed images and live feed viewing.
Enhancing components, such as Fluorescence Filter Cubes have been added (other cubes may be available - please inquire).
This microscope is compatible with slides (options for petri dishes or microplates may be available).
This microscope includes an XY motorized stage, EPI Fluorescence Path Shutter, motorized Objective Nosepiece, motorized fluorescence cube turret, and motorized port splitter.
The fluorescence light source included is a Lumencor Aura fluorescence source

Suited for the following applications as configured:

Cell Culture Imaging: ideal for observing live cells, organoids, and cellular morphology in both Brightfield and Phase Contrast.
Immunofluorescence Studies: suitable for detecting fluorescently labeled proteins, antibodies, and other biomolecules using reflective fluorescence techniques.
Cancer Research: effective for monitoring cell behavior, apoptosis, or proliferation in Brightfield and fluorescence modes.
Drug Screening: enables evaluation of drug effects on live or fixed cells using fluorescence or phase contrast imaging.
Stem Cell Research: facilitates tracking of differentiation, morphology, and viability in various microscopy modes.
Microbiology: useful for studying bacterial growth, motility, and biofilms in Brightfield or fluorescence.
Molecular Biology: supports visualization of nucleic acids, proteins, or organelles tagged with fluorescent dyes.
Tissue and Histological Studies: enables imaging of thin tissue slices in Brightfield and enhanced visualization with fluorescence labeling.
Neuroscience Applications: suitable for imaging neural networks and synaptic activity using fluorescence microscopy.

Other potential applications:

Live-cell imaging: tracking dynamic cellular processes.
Developmental biology: studying tissue morphogenesis and differentiation.
Virology research: observing virus-host interactions.
3D cultures/organoids: imaging structural and molecular features.
Nanoparticle tracking: monitoring fluorescently labeled particles.
Genetics/CRISPR: visualizing genetic edits or modifications.
Pathology: identifying biomarkers in clinical research.
Protein dynamics: assessing protein interactions and localization.
Forensic analysis: detecting fluorescent evidence traces.

Brochure: Nikon Eclipse Ti2-E Microscope brochure
Manual: Nikon Eclipse Ti2-E Microscope manual

Video Overview:

Check out our microscopy capabilities:

https://www.bostonind.com/our-microscopy-capabilities

How we pack

Why do so many customers choose to order from us?

We make sure to match the microscope capabilities to your requirements.
We have experience servicing and repairing these microscopes.
This microscope goes through an extensive set of service steps before shipping.
We offer detailed photos and videos of the microscopes you buy.
We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
Many upgrade and repair parts are in stock.
Remote installation and microscope operation training are available.
On-site installation may be available in the Boston Area (New England area)
Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Warranty
An extended warranty may be available.
You get after-sales technical support.
Customer references are available.
The microscope will be complete and working!
Smooth process from the initial quote to order fulfillment.

*SM* Small Crate
300#

COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW

What steps do you take to verify, service, and confirm the functionality of the Nikon Eclipse Ti2-E Microscope?

We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.

We confirmed the microscope's physical condition.
We meticulously inspected and cleaned the optical path within the microscope.
We lubricated mechanical components.
We thoroughly tested the halogen lamp and light source to confirm their functionality.
We tested the TI2-CTRE power supply.
We inspected and confirmed the clarity and performance of the objectives.
We tested the fluorescence system, ensuring the source operates at optimal efficiency.
We evaluated the port-splitter to confirm seamless image switching between the camera and eyepieces.
We checked the mechanical condenser for smooth operation.
We tested the motorized Z-axis for precise movement.
We validated the motorized objective turret for accurate performance.
We tested the motorized stage to ensure precise and responsive motion across the XY axis.
We confirmed the motorized fluorescence turret operates with accurate alignment.
We tested the motorized fluorescence shutter to verify smooth operation.
We confirmed the connection between the camera and computer for stable and uninterrupted communication.
We tested the live feed, ensuring it delivers clear and high-resolution imaging.
We comprehensively assessed all motorized components, including the objective turret, Z-axis, XY stage, and shutters.
We confirmed that the eyepieces and camera port produce clear, sharp images.
We verified that all mechanical and motorized components operate smoothly, enabling effortless sample focusing and adjustment.
We ensured necessary accessories and cables are included.

Can you help me identify the best microscope and configuration for me?

We are here to help.

Contact us with your application; we will help you identify the best microscope configuration.
We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.

Can I view the Nikon Eclipse Ti2-E Microscope in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.

What about the warranty? Installation, training, maintenance?

Warranty Terms:

The microscope is fully warranted for parts and labor.
The customer should retain all packing materials during the warranty period.

Installation & Training:

Option 1 – In Person

If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.

Option 2 – Remote/Virtual

Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.

Both training options include:

Review of the microscope and its capabilities.
Microscope and software operation.
Review of the recommended maintenance procedures.

We generally recommend these training services to anyone who is new to this microscope model.

What should I watch out for when buying a Nikon Eclipse Ti2-E Microscope used/pre-owned from another lab?

Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
Confirm that there is no physical damage or any misaligned internal components.
Ask what accessories are included.
Ask how long the warranty is and what it covers.

Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.

We confirm the microscope's physical condition.
We meticulously inspect and clean the optical path within the microscope.
We assess light quality using various fluorescence and non-fluorescence samples.
We ensure the stage moves smoothly in both fine and coarse adjustments.
We ensure the smooth operation of all mechanical components.
We confirm that all electronic/motorized components are working well.
We set up communication between the microscope, installed components, and the software.
We test the camera by acquiring sample images.
We confirm the responsiveness of any included touchscreens and buttons.
We ensure necessary accessories and cables are included.

We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.

Do you have a Nikon Eclipse Ti2-E Microscope on hand?

Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this microscope model elsewhere, possibly for less.

Here are some of the reasons for our price.

Our microscope will be fully serviced.
It will be customized and configured to your requirements.
We may offer longer standard warranty terms.
We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
We invest considerable time into the testing and service of each microscope we sell.
We have to purchase any missing required accessories to make the microscopes complete.
We have to procure and stock service parts to fulfill our warranty.
We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
We guarantee that the microscope will be functional.

This procurement is for a University or College. Are purchase orders with NET terms accepted?

If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
POs are accepted from US and Canadian universities.

How fast will you ship?

We can ship within a few days to 2 weeks, depending on your required microscope customization.

Will my microscope be insured during shipping?

All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

How do you pack? Have you previously packed and shipped a Nikon Eclipse Ti2-E Microscope? Do you ship Internationally?

Packing

Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
Accessories are packed safely in boxes with padding and then safely packed inside the crate.
We may not use a crate for local deliveries to save you money *if delivered by us.
We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile microscopes globally.

Our experience and process

We have safely packed and shipped thousands of sensitive analytical instruments.

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
Know that your microscope was tested and working the day it left our facility and is covered if there are faults.

Sustainability

Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

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