Nikon Eclipse Ti2-E PFS Inverted LED Fluorescence Motorized Microscope (New Filters and Perfect Focus System)

Nikon Eclipse Ti2-E PFS Inverted LED Fluorescence Motorized Microscope (New Filters and Perfect Focus System)

	Approved Vendor to many US, Canada, and EU Universities
	US & International Shipping
	Hundreds of Custom Microscopes Shipped
	Customer References Available
	Training and Installation Options Available

Stock: In Stock
Brand: Nikon
Product Code: SM100198-XY
Condition: Pre-Owned

$54,495.00

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For Sale Nikon Eclipse Ti2-E PFS Inverted LED Fluorescence Motorized Microscope

Fully Functional
Serviced & in Stock
Customized to your requirements
Includes new fluorescence filters
Technical sales consultation available
Continental USA 180 DAYS WARRANTY & 60 DAYS INTERNATIONAL
Includes remote microscope training session and software overview

Service Highlights

Optics quality is checked for field distortion and uniformity at the specimen plane
Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
Preventive Maintenance (PM) is Completed

More info about our Microscopy Capabilities or view the Microscope Buying Guide

This is what you will receive before customizing this microscope:

Standard Nikon Eclipse Ti2-E PFS (Perfect Focus System) Microscope Configuration:

Microscopy Techniques:

Brightfield and Fluorescence Microscopy

Microscope Base:

Nikon Ti2-E PFS (Perfect Focus) Inverted Microscope
Nikon TI2-CTRE Control Box

Microscope Head:

TC-T-TS Binocular Head
CFI 10x/22 Eyepiece (Qty. 2)

Fluorescence Light Path:

Liquid Light Guide
Reflective Light Path

Fluorescence Illumination:

Lumencor LED Spectra Multicolor Light Engine

Fluorescence filter cube turret:

Fluorescence Cube Motorized 6-position turret TI2-F-FLT-E

Fluorescence filters:

Three empty filter cubes
Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3
*Other filters available - please inquire*

Brightfield Illumination:

Pillar for transmitted illumination TI2-D-PD
LED Lamphouse for dia illumination TI2-D-LHLED

Objective Turret:

Perfect Focus Unit with motorized 6-position nosepiece (Ti2-N-ND-P)

Objectives:

4x/0.13 Plan Fluor
10x/0.30 Plan Fluor
20x/0.75 Plan APO VC DIC

Condenser:

Mechanical Condenser Turret
LWD Manual Condenser Lens with 0.52 Lens

Stage:

Nikon TI2-CTRE Control Box
ProScan III H31XYZE Microscope Controller
Prior ProScan H117P1N4/F Motorized Stage
PRIOR CS200XY Joystick

Photo/Port Splits:

Left - 100%
Right - 100%
Eyepieces - 100%
Eyepieces/Left - 20%/80%

Camera:

Bestscope 8.3MP color CMOS camera

Computer & Software:

Computer running applicable software

Accessories:

Necessary power and communication cables

This configuration includes any 3 of these new filter sets.

New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

Automated/motorized capabilities of this microscope:

Installed automation/motorized components:

Motorized XY Stage
Motorized Objective Nosepiece
Motorized Fluorescence Filter Turret
Motorized Z axis with drift control PFS

This microscope's imaging capabilities:

Imaging software highlights:

Ability to take still images and live videos of your samples
Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
Variable linear measurements such as line segment, radius values, and others
Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
Multi-frame (Time lapse/Image acquisition over time)
*Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage

We can customize this microscope to fit your requirements:

Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)

Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality:

Microscope can be configured for magnification between 40x and 200x (with 10x Eyepieces)
We can add standard higher magnification Nikon microscope objectives
We can add high-end Nikon Apochromat objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

Additional filter cubes
Additional filter sets to match your fluorochromes.
Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:

We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

We can configure it to work with the sample medium you need:

Slide, Petri dish, microplate, etc.

Commonly Requested Customer Upgrades

High-End CCD, sCMOS Cameras
LED or Metal Halide Fluorescence Light Sources
Phase Contrast, Polarization, or the DIC configurations
Higher Magnification Objectives
Higher performance Fluor or APO objectives

We are excited to help build and configure the right microscope for you!

Nikon Eclipse Ti2-E PFS Microscope Overview

(Final microscope capabilities will depend on the modifications you request)

Inverted Nikon Ti2-E PFS research microscope supporting brightfield and fluorescence imaging.
Motorized components include 6-position objective turret and 6-position fluorescence filter cube turret.
LED-based illumination for both transmitted light and fluorescence using Lumencor Spectra light engine.
Configurable fluorescence light path with liquid light guide and support for common dye filter sets.
Precision motorized stage with joystick control enabling accurate XY positioning and Z focus control.
Modular optical design allowing future upgrades to optics, illumination, detectors, and automation.

Suited for the following applications as configured:

Routine cell culture imaging and inspection in transmitted light
Fixed-cell fluorescence imaging using DAPI, FITC, Texas Red, or Cy3 filters
Multicolor fluorescence assays with manual or motorized filter switching
General life science research and laboratory documentation
Teaching and training in brightfield and fluorescence microscopy
High-resolution imaging of adherent cells on standard culture vessels

PFS System overview

Automatic focus stability that maintains precise focus over long imaging sessions
Real-time drift correction that compensates for temperature and mechanical changes
Near-infrared focus tracking and no interference with visible or fluorescence imaging
Ideal for time-lapse imaging and ensures consistent results across hours or days
Non-invasive to samples and no added photobleaching or phototoxicity

Other potential applications:

Live-cell imaging with additional environmental control and incubation upgrades
Time-lapse microscopy with extended automation and autofocus integration
High-content screening with camera and software expansion
Phase contrast or DIC imaging with added optics and condensers
Advanced multichannel fluorescence with expanded filter sets and light sources
Quantitative imaging and image-based assays with upgraded detectors and analysis tools

Brochure: Nikon Eclipse Ti2-E PFS Microscope brochure
Manual: Nikon Eclipse Ti2-E PFS Microscope manual

Video Overview:

Check out our microscopy capabilities:

https://www.bostonind.com/our-microscopy-capabilities

How we pack

VIEW SOME OF THE CUSTOMERS WE HELPED HERE

REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US

They want to ensure the microscope is customized to fit their application
They want to ensure that internal optics are properly cleaned
They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
They want to ensure that image quality is verified at the sample point for field distortion and uniformity
They want to ensure that the fluorescence intensity is verified at the sample point
They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
They want training to learn how to operate the microscope
They want a reliable warranty
They want peace of mind knowing it will be safely and professionally packed for shipping

We make sure to match the microscope capabilities to your requirements.
We have experience servicing and repairing these microscopes.
This microscope goes through an extensive set of service steps before shipping.
We offer detailed photos and videos of the microscopes you buy.
We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
Many upgrade and repair parts are in stock.
Remote installation and microscope operation training are available.
On-site installation may be available in the Boston Area (New England area)
Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Warranty
An extended warranty may be available.
You get after-sales technical support.
Customer references are available.
The microscope will be complete and working!
Smooth process from the initial quote to order fulfillment.

*SM* Small Crate
300#

COMMON CUSTOMER QUESTIONS AND ANSWERS BELOW

What steps do you take to verify, service, and confirm the functionality of the Nikon Eclipse Ti2-E PFS Microscope?

We verify various microscope functions individually and together, performing necessary repairs or maintenance to bring the microscope to our quality standards.

We inspected the Nikon Ti2-E PFS inverted microscope base and confirmed proper operation of the control box.
We examined the binocular head and calibrated both eyepieces.
We evaluated the fluorescence light path, including the liquid light guide, collimating adapter, and reflective optics.
We measured output stability and spectral performance of the light engine.
We exercised and validated the motorized fluorescence filter cube turret across all positions.
We inspected the built-in brightfield illumination path to confirm uniformity and optical alignment.
We evaluated transmitted illumination using the illumination pillar and verified output performance of the LED lamphouse.
We aligned, and confirmed accurate positioning of the motorized six-position objective turret.
We evaluated resolution, contrast, and phase performance of the objectives.
We aligned the mechanical condenser turret and optimized the long-working-distance manual condenser lens.
We checked operation of the ProScan III controller and the motorized stage.
We verified accuracy, repeatability, and responsiveness of the joystick-based stage control.
We confirmed correct light distribution across all photo port and eyepiece split configurations.
We acquired and reviewed test images using the BestScope camera to confirm imaging performance.
We confirmed stable operation of the computer system and verified correct functionality of the applicable software.

Can you help me identify the best microscope and configuration for me?

We are here to help.

Contact us with your application; we will help you identify the best microscope configuration.
We do not believe in a one-size-fits-all approach; you should get a microscope that will do what you need.

Can I view the Nikon Eclipse Ti2-E PFS Microscope in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the microscope you are interested in.

What about the warranty? Installation, training, maintenance?

Warranty Terms:

The microscope is fully warranted for parts and labor.
The customer should retain all packing materials during the warranty period.

Installation & Training:

Option 1 – In Person

If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.

Option 2 – Remote/Virtual

Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.

Both training options include:

Review of the microscope and its capabilities.
Microscope and software operation.
Review of the recommended maintenance procedures.

We generally recommend these training services to anyone who is new to this microscope model.

What should I watch out for when buying a Nikon Eclipse Ti2-E PFS Microscope used/pre-owned from another lab?

Make sure the microscope is properly packed for shipping and will arrive safely. If the microscope has many components, request to have it packed in a wooden crate.
Confirm that there is no physical damage or any misaligned internal components.
Ask what accessories are included.
Ask how long the warranty is and what it covers.

Here is what our technical team tests so you can request something similar and ensure your microscope will be fully functional.

We confirm the microscope's physical condition.
We meticulously inspect and clean the optical path within the microscope.
We assess light quality using various fluorescence and non-fluorescence samples.
We ensure the stage moves smoothly in both fine and coarse adjustments.
We ensure the smooth operation of all mechanical components.
We confirm that all electronic/motorized components are working well.
We set up communication between the microscope, installed components, and the software.
We test the camera by acquiring sample images.
We confirm the responsiveness of any included touchscreens and buttons.
We ensure necessary accessories and cables are included.

We recommend inspecting the microscope in person with your sample or requesting a virtual demo; this will allow you to evaluate the condition and performance of the microscope you are considering purchasing.

Do you have a Nikon Eclipse Ti2-E PFS Microscope on hand?

Yes, this is a common microscope model we carry, and we stock multiple accessories and many replacement parts to back up our warranty.

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this microscope model elsewhere, possibly for less.

Here are some of the reasons for our price.

Our microscope will be fully serviced.
It will be customized and configured to your requirements.
We may offer longer standard warranty terms.
We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
We have to employ technical staff that can do the testing/repairs and after-sales support if there are any issues.
We invest considerable time into the testing and service of each microscope we sell.
We have to purchase any missing required accessories to make the microscopes complete.
We have to procure and stock service parts to fulfill our warranty.
We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
We guarantee that the microscope will be functional.

This procurement is for a University or College. Are purchase orders with NET terms accepted?

If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
POs are accepted from US and Canadian universities.

How fast will you ship?

We can ship within a few days to 2 weeks, depending on your required microscope customization.

Will my microscope be insured during shipping?

All microscopes shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

How do you pack? Have you previously packed and shipped a Nikon Eclipse Ti2-E PFS Microscope? Do you ship Internationally?

Packing

Smaller microscopes ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
Most larger microscopes ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
Accessories are packed safely in boxes with padding and then safely packed inside the crate.
We may not use a crate for local deliveries to save you money *if delivered by us.
We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile microscopes globally.

Our experience and process

We have safely packed and shipped thousands of sensitive analytical instruments.

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.
You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
Know that your microscope was tested and working the day it left our facility and is covered if there are faults.

Sustainability

Well, this one is easy: buying a pre-owned microscope is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

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