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![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | International Shipping |
![]() | Customer References Available |
![]() | Training and Installation Options Available |
- Stock: In Stock
- Brand: Eppendorf
- Product Code: SM101412-SU20
- Condition: Pre-Owned
For Sale Eppendorf BioFlo 120 Bioprocess Bioreactor Control System for Eppendorf Single-Use BioBlu Vessels
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
- Includes remote instrument setup and training session
Bioreactors are configured to meet individual customer requirements (shown with a single-use Eppendorf BioBLU vessel)
We will be able to configure this bioreactor for either microbial production or bioprocessing
Below is the configuration of the Bioflo 120 controller you will receive
Compatible vessel types and volumes
| Bioflo 120 controller can be paired | Eppendorf BioBlu Single-Use vessels are BioBLU 1c, 1f, 3f, 10f, (3c, 5c, 5p, 14c, or 50c either µ or M) |
| Bioflo 120 controller can be paired | Glass/Autoclavable vessels are 1.0L, 2.0L, 5.0L, or 10 Liters. |
Gas flow supply connections
| Gas flow control | 1 TMFC (Thermal Mass Flow Controller) |
| Gas mixing option | 4 gases (Air, N2, O2, CO2) |
| (Gas-in) Supply ports | (Air, O2 , N2 , CO2 ) Maximum 10 psig (0.69 barg) when paired with glass / autoclavable vessels Maximum 6 psig (0.44 barg) when paired with a BioBlu Single-use vessel |
| (Gas-out) Sparge connection | 1 TMFC; Flow Range: (0.04 – 20 SLPM) Also available as TMFC (0.002 – 1 SLPM or 0.01 – 5 SLPM; please inquire for available stock) |
Bioflo 120 Controller digital connections/inputs
| Vessel drive | Accepts direct or magnetic drive connection port |
| Temperature sensor | Accepts PT100 temperature probes |
| Extra digital sensors (Two) | Accepts PH, DO, Redox, or CO2 sensors (maximum 2 sensors) |
| Heater blanket | Allows the controller to manage the blanket's heating function |
| Exhaust condenser | Accepts different-sized Peltier exhaust condensers |
| Level Sensors | One available level input |
| Optional: | (3) three analog input/output connections on the advanced model |
Bioflo 120 Controller peristaltic pumps
| Includes 3 installed peristaltic pumps | |
| Pumps 1, 2, & 3 / Watson-Marlow 114DV head | with Fixed Speed: 30 rpm (0 – 100% Duty Cycle) |
Installed liquid connections and their operating temperature ranges
| External water source | Main water supply and return/drain |
| Vessel supply and return |
|
| Liquid-cooled exhaust condenser supply & return | 0.5 psig (0.035 barg) |
Controller Weight/Dimensions/Power Requirement
| Power requirements | 100-120/208-240 VAC |
| Dimensions (W x D x H) | 24.7 × 55.9 × 62.9 cm (9.7 × 22 × 24.8 in) |
| Net weight: | 14.8 kg (32.7 lb) |
Here is what you will receive:
- Eppendorf Bioflo 120 Bioprocess Control Station (for BioBlu vessels)
- Drive (matching the vessel volume size and type) (Qty:1)
- 4-gas regulator w/ valves (Qty:1)
- Ph sensor cable (Qty:1)
- Ph sensor (Qty:1)
- DO (Disolved Oxygen) Sensor (Qty:1)
- Temperature Sensor (Qty:1)
You can pair this Bioflo 120 controller with single-use Eppendorf BioBLU vessels for cell culture or microbial applications, including Bacteria, Yeast, Fungi, Mammalian(Animal), or Insect cells.
Above is a kit for use with BioBLU vessels
* Heated blanket will be included free if you will be using this bioreactor with BioBLU vessels; please inquire about the size you will need
Eppendorf BioBlu c single use-vessels – (Cell Culture) (Total Volume) | BioBLU 1c (1.8 L) | BioBLU 3c (5 L) | BioBLU 5c (5 L) | BioBLU 5p (5 L) | BioBLU 14c (14 L) | BioBLU 50c (50 L) |
| Working volume | 320 mL – 1.25 L | 1.25 – 3.75 L | 1.25 – 3.75 L | 3.75 L | 3.5 – 10.5 L | 18 – 40 L |
| Number of impellers: | 1x or 2x pitched-blade | 1x or 2x pitched-blade | 1x pitched-blade | packed-bed | 1x pitched-blade | 1 x pitched-blade |
| Drive: | Magnetic overhead drive | |||||
OR
Eppendorf BioBlu f single use-vessels – (Fermentation) (Total Volume) | BioBLU 1f (1.8 L) | BioBLU 3f (5 L) |
| Working volume: | 250 mL – 1.25 L | 1.25 – 3.75 L |
| Number of impellers: | 2 or 3 x Rushton-type | 3 x Rushton-type |
| Drive: | Magnetic overhead drive | |
* Please note we also have other Bioflo 120 and Bioflo 320 with autoclavable glass vessels.
* Should you need multiple bioreactors, we generally stock multiple units of each model
Reactor Customization/Options might be available upon request. Please inquire with our sales team.
- Different Volume Vessels
- Choice of agitation impellers (pitched blade, marine, Rushton)
- Following range TFMC mass flows may be available: 0.002 – 1 SLPM, 0.01 – 5 SLPM, 0.04 – 20 SLPM
- External chiller (heating/cooling)
- Cooling loops or cooling fingers for temperature control
- Redox or CO2 sensors
- Exhaust condenser (liquid or peltier)
- Jacketed vessels
- Sparge vessel attachment
- Vessel Baffle
- Level / Foam sensor
- Others accesories

Please note that, depending on the requested options, we may sometimes recommend a different model bioreactor that may better fit your application.
Testing performed by our in-house service team:
The Bioflo 120 Bioprocess Controller passes the following tests:
- We verify the power supply and complete the system initialization process.
- We assess the functionality of the touchscreen display, confirming responsive and accurate input.
- We test the agitation motor and motor control, confirming smooth and stable operation at multiple RPM ranges.
- We test Pumps 1 through 3 to ensure reliable and accurate delivery rates, including external pumps added to the system.
- We verify the pH sensor readings using a calibration solution, confirming accurate output.
- We verify the dissolved oxygen (DO) sensor readings using a calibration solution, ensuring sensor accuracy.
- We test the heating control system and verify temperature accuracy using a NIST-traceable calibrated probe.
- We test the gas output via the sparge line and verified gas control functionality.
- We test all add-on options and accessories.
- We perform additional testing as requested within reasonable parameters.
Instrument Overview:
- The Eppendorf BioFlo 120 Bioprocess Control System is designed for microbial and cell culture applications.
- It supports single-use and glass vessels ranging from 250 mL to 40 L in volume.
- Integrated touchscreen interface enables streamlined control of key parameters like pH, DO, temperature, and agitation.
- BioFlo 120 offers automated gas mixing and pump control for enhanced process precision.
- Built-in peristaltic pumps simplify fluid handling and media addition during cultivation runs.
- The system ensures reproducible results with accurate sensor calibration and reliable control components.
Instrument Application:
- Cell line development
- Protein expression
- Vaccine production
- Fermentation of microorganisms
- Media optimization
- Small-scale biomanufacturing
The Key Features:
- Compact, space-saving design
- Built-in touchscreen interface
- Automated gas and pump control
- Accurate sensor calibration tools
- Supports single-use and glass vessels
Video Overview:
About us

How we pack

VIEW SOME OF THE CUSTOMERS WE HELPED HERE
REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US
- They want to ensure that the bioreactor controller has been thoroughly tested and responds to commands
- They want to ensure that the paired drive will be correctly matched to the required vessel size and type
- They want to ensure that the vessel’s drive RPMs have been verified against the tachometer
- They want to ensure that the bioreactor controller can detect all attached sensors
- They want to ensure that all peristaltic pumps are tested up to their maximum RPMs
- They want to ensure that the internal heating coil and fluid pump are performing properly
- They want a reliable warranty
- They want to ensure that the included vessel is correctly matched to the bioreactor controller
- They want peace of mind knowing it will be safely and professionally packed for shipping

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

- Warranty
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*SM* Small Crate
315#
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Bioflo 120 Bioprocess Controller?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards..
- We verify the power supply and complete the system initialization process.
- We assess the functionality of the touchscreen display, confirming responsive and accurate input.
- We test the agitation motor and motor control, confirming smooth and stable operation at multiple RPM ranges.
- We test Pumps 1 through 3 to ensure reliable and accurate delivery rates, including external pumps added to the system.
- We verify the pH sensor readings using a calibration solution, confirming accurate output.
- We verify the dissolved oxygen (DO) sensor readings using a calibration solution, ensuring sensor accuracy.
- We test the heating control system and verify temperature accuracy using a NIST-traceable calibrated probe.
- We test the gas output via the sparge line and verified gas control functionality.
- We test all add-on options and accessories.
- We perform additional testing as requested within reasonable parameters.
What supplies do I need?
You will need the consumables starting out:
- BioFlo 120 Bioreactors
- BioBLU c Single-Use Bioreactors
- BioBLU f Single-Use Bioreactors
- BioBLU Single-Use Bioreactor Adaptor Kits
Can I view the Bioflo 120 Bioprocess Controller in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Bioflo 120 Bioprocess Controller used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We verify the power supply and complete the system initialization process.
- We assess the functionality of the touchscreen display, confirming responsive and accurate input.
- We test the agitation motor and motor control, confirming smooth and stable operation at multiple RPM ranges.
- We test Pumps 1 through 3 to ensure reliable and accurate delivery rates, including external pumps added to the system.
- We verify the pH sensor readings using a calibration solution, confirming accurate output.
- We verify the dissolved oxygen (DO) sensor readings using a calibration solution, ensuring sensor accuracy.
- We test the heating control system and verify temperature accuracy using a NIST-traceable calibrated probe.
- We test the gas output via the sparge line and verified gas control functionality.
- We test all add-on options and accessories.
- We perform additional testing as requested within reasonable parameters.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a Bioflo 120 Bioprocess Controller on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Bioflo 120 Bioprocess Controller Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
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