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![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | International Shipping |
![]() | Customer References Available |
![]() | Training and Installation Options Available |
- Stock: In Stock
- Brand: Distek
- Product Code: 66664
- Condition: Pre-Owned
For Sale Distek BIOne 1250 Bioreactor System with 2L Autoclavable Cell Culture Vessel and O₂/CO₂/Air Gas Control
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
- Includes remote instrument setup and training session
Here is what you will receive:
- Distek 1250 bioprocess control station
- BIOne 2L autoclavable glass bioreactor with holder
- Drive motor
- Heating blanket
- pH cable with sensor
- DO cable with sensor
- Temperature probe
Distek BIOne 1250 Benchtop Bioreactor System Configuration:
Aeration/Gas Control:
- 4-gas configuration/ 4 independent gas channels with integrated mass flow control
- Air (sparger) for aeration; Air sparger TMFC: max 1000 sccm)
- Air (overlay) for headspace control; Air overlay TMFC: max 1000 sccm)
- O₂ (oxygen) enrichment; O₂ TMFC: max 1000 sccm
- CO₂ for pH control; CO₂ TMFC: max 750 sccm
Pump System: 4 integrated peristaltic pumps (BIOne 1-4)
Variable speed control
Typical uses: acid/base addition; feed/media addition; antifoam dosing
Vessel Type: BIOne 2L autoclavable glass bioreactor (cell culture configuration)
Total volume: 3 L
Working volume: 0.95-2.0 L
Non-jacketed vessel with external heating blanket
Real-time monitoring: temperature, pH, dissolved oxygen (DO), RPM
Agitation System:
External direct-drive motor with encoder feedback. Agitation range: 15-1250rpm
Pitched-blade impeller, axial flow
Low-shear mixing suitable for cell culture applications
Variable speed control via the controller
Temperature Control:
Temperature control via external heating blanket (200 W), ambient to ~60 °C (typical)
External cooling (external chiller required for cooling operation) via:
- Cooling supply and return ports
- Condenser loop (supply/return)
Controller digital connections / Auxiliary Inputs:
- Sensor Ports: 1-3 for pH/Redox; 4 and 5 for DO
- Level sensors (Level 1 / Level 2)
- Scale input (external balance)
- Temperature probe input
- Filter heater connection
- Heater connection
Connectivity & Interfaces:
- Ethernet (network integration)
- RS-232 serial communication
- 2 x USB ports
- The additional I/O enables the incorporation of multiple Process Analytical Technologies (PATs) into the BIOne 1250 Control Station.
- 4 Analog I/O (multi-channel, configurable) Analog Input 1: Optical DO (4–20 mA, saturation units)
- 2 Digital I/O (configurable)
Customization/Options: N/A
Testing performed by our in-house service team:
The Distek BIOne 1250 Benchtop Bioreactor System passed all tests and was found to be fully functional:
- We powered on and initialized the system successfully.
- We verified that the touchscreen interface is responsive and fully functional.
- We evaluated the agitation system and confirmed stable motor operation with smooth speed control.
- We checked the heating blanket and confirmed proper heating response.
- We operated all peristaltic pumps and confirmed proper function and flow control.
- We verified the gas controls using a calibrated MFC and confirmed the proper function of the air, O₂, and CO₂ channels.
- We verified the pH and dissolved oxygen (DO) sensors and confirmed signal response.
Instrument Overview:
- The Distek BIOne 1250 is a fully integrated bench-top bioprocess control system designed for precise control of cell culture and aerobic bioprocess applications.
- This unit is configured with a 2 L autoclavable glass vessel and supports closed-loop control of dissolved oxygen, pH, and temperature.
- The system features a 4-gas configuration (air sparger, air overlay, oxygen, and carbon dioxide), enabling accurate aeration, oxygen enrichment, and pH control via CO₂.
- A servo-driven agitation motor with encoder feedback and a pitched-blade impeller provides low-shear axial mixing optimized for cell culture processes.
- Four integrated peristaltic pumps enable precise, automated dosing of acid, base, feed media, and antifoam for controlled process operation.
- The system includes pH, dissolved oxygen (DO), and temperature probes, along with multiple additional sensor input ports for expanded capability.
- With a built-in touchscreen interface, multiple sensor inputs, and network connectivity, the BIOne 1250 is a reliable platform for laboratory and pilot-scale bioprocessing.
Instrument Application:
- Cell culture process development
- Aerobic bioprocess optimization
- Fed-batch and batch studies
- Media and process optimization
- Scale-up feasibility testing
- Academic and R&D laboratory use
The Key Features:
- 2 L autoclavable glass vessel
- Low-shear pitched-blade mixing
- Integrated heating blanket
- 4-gas system: air overlay, sparger, O₂, CO₂
- 4 peristaltic pumps (base + 3 configurable)
- pH, DO, temperature, and agitation control
Video Overview:
About us

How we pack

VIEW SOME OF THE CUSTOMERS WE HELPED HERE
Why do so many customers choose to order from us?

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*ME* Medium Crate
390#
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Distek BIOne 1250 Benchtop Bioreactor System?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.
- We powered on and initialized the system successfully.
- We verified the touchscreen interface is responsive and fully functional.
- We evaluated the agitation system and confirmed stable motor operation with smooth speed control.
- We checked the heating blanket and confirmed proper heating response.
- We operated all peristaltic pumps and confirmed proper function and flow control.
- We verified the gas controls using a calibrated MFC and confirmed the proper function of the air, O₂, and CO₂ channels.
- We verified the pH and dissolved oxygen (DO) sensors and confirmed signal response.
Can I view the Distek BIOne 1250 Benchtop Bioreactor System in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Distek BIOne 1250 Benchtop Bioreactor System used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We powered on and initialized the system successfully.
- We verified the touchscreen interface is responsive and fully functional.
- We evaluated the agitation system and confirmed stable motor operation with smooth speed control.
- We checked the heating blanket and confirmed proper heating response.
- We operated all peristaltic pumps and confirmed proper function and flow control.
- We verified the gas controls using a calibrated MFC and confirmed the proper function of the air, O₂, and CO₂ channels.
- We verified the pH and dissolved oxygen (DO) sensors and confirmed signal response.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a Distek BIOne 1250 Benchtop Bioreactor System on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Distek BIOne 1250 Benchtop Bioreactor System Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
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