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We apologize this Instrument is currently | |
Please submit a request, we will notify you if another one comes in stock. Thank you! | |
![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | US & International Shipping |
- Stock: SOLD
- Brand: Eppendorf
- Product Code: 58239
- Condition: Pre-Owned
For Sale Eppendorf BioFlo 120 Bioprocess Control Station, (TMFC 20 SLPM) w/ 3-Liter Glass Vessel
This item has been recently sold. Please view the other available instruments on your right.
If you do not see what you need, let us know via the “Request Quote” form; we often replenish sold-out items.
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- WARRANTY
Please note that a green dye was intentionally used for the video to better show the operation.
Here is what you will receive:
- Eppendorf BioFlo 120 Bioprocess Controller
- Eppendorf 3 Liter Glass Vessel (2.2 Liter Working Volume)
- Heated Blanket for 3-liter Glass Vessel with Stainless Steel Headplate
- Overhead Direct Drive Motor, 25-1200 RPM (PN: ESM80K-C1)
- Ph/ReDox sensor cable PN: 1390810400
- DO sensor cable PN: M1379-8107
- Pt100 Temperature probe
- Level sensor connection cable PN: 1390811000
- Water Connection Kit: Condenser Water In & Out Tubing, Vessel Water In & Out Tubing
3-Liter Glass Vessel Includes:
- Direct Drive Impeller Shaft with x2 Rushton Impeller
- Exhaust Condenser with Cooling Inlet and Outlet
- Submerged Cooling Loop for Vessel Temperature Control with Inlet and Outlet
- Removable Stainless Steel Baffle Insert, fermentation only
- Ring Sparge with Sparge Supply Tubing
- Triple Feed Port with Luer-Lock Fittings
- Single Feed Port with Luer-Lock Fitting
- Thermowell for Temperature Sensor
- Temperature Sensor
- Sampling Port with Luer-Lock Fitting
- Various Blindplugs for Headplate
BioFlo 120 Bioreactor Configuration:
- Vessel: Eppendorf 3-Liter Glass Vessel (working volume 0.8-2.2 L)
- Temperature Range:70DegC maximum
- Temperature Control: external heating blanket and cooling loop
- Temperature Sensor Type: Pt100
- Gas mixing options: 4 gas (air, N2, O2, CO2)
- Gas flow control: Thermal Mass flow Controller (TMFC): 0.04 - 20 SLPM
- Agitation range with direct drive: 25 - 1200 RPM
- Pumps 1, 2, & 3: Watson-Marlow 114DV head, 30rpm (0-100% duty cycle)
- Dimensions (H x W x D): 24.7 x 55.9 x 62.9 cm (9.7 x 22 x 24.8 in)
- Power requirements: 50/60 Hz,100-120V/208-240V, 10A
Customization/Options: N/A
Testing performed by our in-house service team:
The BioFlo 120 Bioreactor passed all tests and was found to be fully functional:
- We verified the power supply and completed the initialization process.
- We assessed the functionality of the touchscreen display.
- We tested the agitation motor and motor control, confirming the smooth operation.
- We tested and calibrated pumps 1-3 to ensure accurate performance.
- We verified the readings of the PH sensor using our calibration solution.
- We verified the readings of the DO sensor using our calibration solution.
- We tested the vessel's heated jacket and heating control and verified the temperature using our NIST-calibrated probe.
- We confirmed the heated jacket shutoff after reaching the temperature setpoint.
- We tested the operation of the condenser and cooling loop solenoids.
- We checked the gassing output (sparge) and gas control.
- We monitored the trend graph and data logging.
Instrument Overview:
- The Eppendorf BioFlo 120 is a compact and versatile bioprocess control station for lab-scale fermentation and cell culture.
- It supports multiple vessel sizes and types, including single-use and autoclavable glass vessels from 250 mL to 40 L.
- This system provides precise control of key parameters like pH, dissolved oxygen, temperature, and agitation.
- Its modular design allows easy integration of additional sensors and accessories to meet diverse process requirements.
- The BioFlo 120 offers a user-friendly interface with customizable settings for various bioprocess workflows.
- It is ideal for research and development in biotechnology, pharmaceuticals, and academic laboratories.
Instrument Application:
- Fermentation and cell culture monitoring and control
- Microbial fermentation for enzyme or biofuel production
- Cell culture for protein expression or vaccine development
- Scale-up studies for biopharmaceutical processes
The Key Features:
- Compact design for lab-scale bioprocesses
- Supports single-use and glass vessels from 250 mL to 40 L
- Modular setup for sensor customization
- Heated jacket for accurate temperature regulation.
- Thermal Mass flow Controller as a gassing option
- Agitation speeds up to 1500 RPM with direct drive (vessel dep-nt)
- Easy-to-use interface for smooth operation
Video Overview:
About us

How we pack

Why do so many customers choose to order from us?

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

- Warranty
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*SM* Small Crate
220#
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the BioFlo 120 Bioreactor?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards..
- We verified the power supply and completed the initialization process.
- We assessed the functionality of the touchscreen display.
- We tested the agitation motor and motor control, confirming the smooth operation.
- We tested and calibrated pumps 1-3 to ensure accurate performance.
- We tested the vessel's heated jacket and heating control.
- We confirmed the heated jacket shutoff after reaching the temperature setpoint.
- We tested the operation of the condenser and cooling loop solenoids.
- We checked the gassing output (sparge) and gas control.
- We monitored the trend graph and data logging.
Can I view the BioFlo 120 Bioreactor in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warrantied for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a BioFlo 120 Bioreactor used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We verified the power supply and completed the initialization process.
- We assessed the functionality of the touchscreen display.
- We tested the agitation motor and motor control, confirming the smooth operation.
- We tested and calibrated pumps 1-3 to ensure accurate performance.
- We tested the vessel's heated jacket and heating control.
- We confirmed the heated jacket shutoff after reaching the temperature setpoint.
- We tested the operation of the condenser and cooling loop solenoids.
- We checked the gassing output (sparge) and gas control.
- We monitored the trend graph and data logging.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a BioFlo 120 Bioreactor on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped BioFlo 120 Bioreactor Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
