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Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 

Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

  • Stock: In Stock
  • Brand: Biotek Bio-Tek
  • Product Code: 67954
  • Condition: Pre-Owned
$28,995.00
CLICKABLE TABS BELOW

For Sale Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader 

 

  • Fully Functional
  • Serviced & in Stock
  • Current Model
  • Made in 2024
  • Good cosmetic condition
  • Technical sales consultation available
  • WARRANTY
  • Includes remote instrument setup and training session

 


Here is what you will receive:

  • Agilent BioTek Cytation 5 CYT5MF Multinmode Microplate Reader
  • Custom filter cube (Position1- Ex:485/20nm Dm:510nm Em:530/25nm)(Position2 - Ex:440/30nm Dm:510nm Em:530/25nm)
  • Power supply and Communication cables
  • Desktop Computer running Gen5 software
  • Monitor, Mouse, and Keyboard

 

Cytation 5 CYT5MF Multimode Microplate Reader Configuration:

  • Multimode-only configuration (no imaging)
  • Detection Modes (Monochromator-Based or Filter based): UV-Vis absorbance, Fluorescence intensity, Time-resolved fluorescence (secondary mode), Luminescence
  • Optical System: Quad monochromators (top/bottom for FI; secondary for TRF)
  • Fluorescence Intensity wavelength range: 250-700 nm
  • Variable bandwidth: 9-50 nm in 1 nm increments
  • Fluorescence dynamic range: 7 decades
  • Reading speed: 96 wells in 11 s; 384 wells in 22 s
  • Absorbance wavelength range: 230-999 nm
  • Bandwidth: 4 nm (230-285 nm); 8 nm (>285 nm)
  • Dynamic range: 0-4.0 OD Resolution: 0.0001
  • Wavelength accuracy: ± 2 nm, repeatability: ± 0.2 nm
  • Luminescence wavelength range: 300-700 nm
  • Luminescence dynamic range: > 6 decades
  • Environmental Control: 4-zone incubation up to 65 DegC with Condensation Control
  • Temperature uniformity: ± 0.2 DegC at 37 DegC
  • Plate Compatibility: 6-384 well microplates
  • Shaking: linear, orbital, double orbital
  • Dimensions: 16.4" W × 17.5" H × 20.2" D
  • Weight: 80 lb (36.3 kg)

 

Customization/Options: N/A

 

Testing performed by our in-house service team:

The Cytation 5 CYT5MF Multimode Microplate Reader passed all tests and was found to be fully functional:

  • We verified proper power operation and successful system initialization.
  • We confirmed reliable communication with the control software.
  • We tested incubation performance up to the maximum specified temperature of 65 °C.
  • We evaluated all available shaking modes and confirmed proper operation.
  • We confirmed accurate absorbance measurements using a validated test plate.
  • We confirmed proper fluorescence detection using a validated test plate.
  • We confirmed proper luminescence detection using a validated test plate.
  • We tested and confirmed proper time-resolved fluorescence (TRF) performance using a validated test plate.
  • We verified the functionality and accuracy of the auto-exposure feature.

 

Instrument Overview:

  • Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader is a filter- and monochromator-based detection system without imaging.
  • Offers filter-based modes including fluorescence intensity, polarization, time-resolved fluorescence, luminescence, and alpha detection.
  • Supports monochromator-based UV–Vis absorbance, fluorescence intensity, time-resolved fluorescence, and luminescence.
  • Provides dual-readout flexibility with both filter-based and monochromator-based measurement pathways.
  • Designed for compatibility with microplates and Take3 microvolume plates for varied sample formats.
  • Delivers broad multimode detection coverage across absorbance, fluorescence, luminescence, and advanced fluorescence variants.

 

Instrument Application:

  • Absorbance assays (e.g., ELISA, protein/DNA quantification)
  • Fluorescence intensity assays
  • Luminescence assays (e.g., ATP, reporter genes)
  • Cell viability and cytotoxicity assays
  • Enzyme activity measurements
  • Kinetic (time-course) plate assays
  • Multiplex biomarker detection

 

The Key Features:

  • UV–Vis, fluorescence, luminescence detection
  • Filter and monochromator dual modes
  • Wide absorbance range: 230–999 nm
  • Fluorescence range: 250–700 nm
  • Rapid reads: 96/well in 11 s
  • 4-zone incubation up to 65 °C

 

 

Additional technical info:

 

Video Overview:

 

About us

 

How we pack

  



 VIEW SOME OF THE CUSTOMERS WE HELPED HERE 


Why do so many customers choose to order from us?

 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping. see the questions section below
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Technical sales consultation available
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 


  • Warranty
  • You get after-sales technical support
  • Customer references are available
  • The instrument will be fully functional!
  • Smooth process from the initial quote to order fulfillment

 


This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What are the steps you take to verify, service, and confirm the functionality of the Cytation 5 CYT5MF Multimode Microplate Reader?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

  • We verified proper power operation and successful system initialization.
  • We confirmed reliable communication with the control software.
  • We tested incubation performance up to the maximum specified temperature of 65 °C.
  • We evaluated all available shaking modes and confirmed proper operation.
  • We confirmed accurate absorbance measurements using a validated test plate.
  • We confirmed proper fluorescence detection using a validated test plate.
  • We confirmed proper luminescence detection using a validated test plate.
  • We tested and confirmed proper time-resolved fluorescence (TRF) performance using a validated test plate.
  • We verified the functionality and accuracy of the auto-exposure feature.

 

What supplies do I need?

You will need the consumables starting out:

 

Can I view the Cytation 5 CYT5MF Multimode Microplate Reader in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.

 

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warranted for both parts and labor.
  • The customer should retain all packing materials during the warranty period.

 

What should I watch out for when buying a Cytation 5 CYT5MF Multimode Microplate Reader used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

  • We verified proper power operation and successful system initialization.
  • We confirmed reliable communication with the control software.
  • We tested incubation performance up to the maximum specified temperature of 65 °C.
  • We evaluated all available shaking modes and confirmed proper operation.
  • We confirmed accurate absorbance measurements using a validated test plate.
  • We confirmed proper fluorescence detection using a validated test plate.
  • We confirmed proper luminescence detection using a validated test plate.
  • We tested and confirmed proper time-resolved fluorescence (TRF) performance using a validated test plate.
  • We verified the functionality and accuracy of the auto-exposure feature.

 

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.

 

Do you have a Cytation 5 CYT5MF Multimode Microplate Reader on hand?
  • This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.

 

Here are some of the reasons for our price.

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We have to purchase any missing required accessories to make the instruments complete.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
  • We guarantee that the instrument will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

 

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

How do you pack? Have you packed and shipped Cytation 5 CYT5MF Multimode Microplate Reader Instruments in the past? Do you ship Internationally?

Packing

  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

 

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.

 

Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

 

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 



We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend tens of thousands of dollars and receive a damaged instrument.

 

Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing. 

 

We generally ship parcels either via UPS or via freight for all larger instruments.

 

Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant).  On freight shipments, we also use dense foam, which provides excellent cushioning and support for the instrument. Instruments then are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provide cushioning in all directions and hold instruments firmly in place.

 

Final step is to we photograph everything inside the crate and then seal it with screws.