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Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1

Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

  • Stock: In Stock
  • Brand: Agilent
  • Product Code: SM101495-INFINITY
  • Condition: Pre-Owned
$21,995.00
CLICKABLE TABS BELOW

For Sale Agilent 5400 Fragment Analyzer (Automation-ready) Unit 1

 

  • Fully Functional
  • Serviced & in Stock
  • Current Model
  • Good cosmetic condition
  • Technical sales consultation available
  • CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
  • Includes remote instrument setup and training session

 


Here is what you will receive:

  • Agilent 5400 Fragment Analyzer
  • (3) Marker/sample PCR microplate (semi- or non-skirted) holders
  • (2) Deep-well microplate holders
  • (1) 50ml bottle
  • (2) 250ml bottle
  • 500ml waste bottle
  • Computer running Fragment Analyzer controller software
  • Communication and power cables

 

Please note: Capillary Arrays and other consumables are not included and are used for demonstrational purposes.

 

Agilent 5400 Fragment Analyzer Configuration:

  • Instrument Applications: DNA and RNA fragment analysis, sizing, quantification, and quality assessment.
  • Analysis Method: automated multiplexed capillary electrophoresis.
  • High Sensitivity: fluorescence-based detection using a high-sensitivity CCD detector and LED excitation.
  • Capillary Array: compatible with 96 capillary arrays
  • Minimum Sample Volume: 2 uL recommended sample input; minimum 20 µL per well for injection.
  • Sample Capacity: 1x96-well PCR sample plate
  • Compatible Microplates: full-skirt 96-well PCR plates for sample and marker preparation; 96-well deep-well plates for buffer and waste reservoirs.
  • Number of Samples per Run: up to 96 samples.
  • Resolution and Sizing Accuracy: up to 3 bp resolution with ≤5% separation resolution and ±10% sizing accuracy
  • Power requirements: 100-240 VAC; 50/60 Hz
  • Dimensions (H x W x D): 86.4 × 101.6 × 61 cm (34 × 40 × 24 in)
  • Weight: 39 kg (86 lb)

 

Customization/Options: 

  • Automation Arms (Please inquire for available inventory)
  • Automated Liquid Handlers (Please inquire for available inventory)

 

Testing performed by our in-house service team:

The Agilent 5400 Fragment Analyzer passed all tests and was found to be fully functional:

  • We check the power supply to ensure it provides stable output.
  • We test communication with the software to verify seamless interaction and data exchange.
  • We test the initialization and homing processes to guarantee accurate and reliable setup.
  • We check pump operation to ensure consistent, reliable fluid movement.
  • We test plate movement to validate precise, smooth sample handling.
  • We thoroughly test the prime function of all fluids to ensure the system is clean and functions as designed.
  • We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
  • We test the high-voltage system from minimum to maximum levels to verify consistent, safe operation.

 

Instrument Overview:

  • Agilent 5400 Fragment Analyzer delivers reliable DNA and RNA quality assessment for high-demand laboratories.
  • High sample capacity helps maximize productivity and accelerate results in busy workflows.
  • Automation-ready design supports efficient, walk-away operation with minimal hands-on time.
  • Generates accurate fragment sizing and quantification for confident decision-making.
  • Flexible configuration adapts easily to changing throughput and performance requirements.
  • Durable, user-focused platform helps reduce operational effort while maintaining consistent results.

 

Instrument Application:

  • DNA fragment sizing and quality assessment
  • NGS library quality control
  • Genomic DNA integrity analysis
  • Total RNA and small RNA evaluation
  • PCR product verification
  • mRNA vaccine characterization and QC

 

The Key Features:

  • High-throughput sample processing
  • Automated capillary separation
  • Accurate fragment sizing
  • Automation-ready workflow
  • Flexible run configuration
  • Minimal hands-on operation

 

Additional technical info:

 

Video Overview:

 

About us

 

How we pack

  



 VIEW SOME OF THE CUSTOMERS WE HELPED HERE 


WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER FRAGMENT ANALYZERS FROM US?

 

  They want to ensure the power supply has been verified for stable output, preventing unpredictable voltage drops or surges that could prevent proper fragmentation of samples.


  They want assurance that a full optical alignment of the capillary array has been performed, verifying that the camera and illumination system are operating properly for accurate detection and baseline stability.


  They want to ensure the fluidic pump operates smoothly and consistently, avoiding pressure fluctuations that can lead to migration failures, sample cross-contamination, or incorrect fragment sizing.


  They want to confirm that plate transport and positioning mechanisms have been validated for smooth, accurate movement, preventing needle crashes, missed wells, or bent sampling probes.


  They want to confirm that electronic noise and EMI levels from the high-voltage supply remain within tolerance and will not distort the image signal quality.


  They want to ensure that the electronics are up to date and compatible with the current software versions.


  They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.


 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping. see the questions section below
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Technical sales consultation available
  • Remote installation and instrument operation training may be available.
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 


  • Warranty
  • An extended warranty may be available
  • You get after-sales technical support
  • Customer references are available
  • The instrument will be fully functional!
  • Smooth process from the initial quote to order fulfillment

 


This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

 

*SM* Small Crate
230#

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What are the steps you take to verify, service, and confirm the functionality of the Agilent 5400 Fragment Analyzer?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

  • We check the power supply to ensure it provides stable output.
  • We test communication with the software to verify seamless interaction and data exchange.
  • We test the initialization and homing processes to guarantee accurate and reliable setup.
  • We check pump operation to ensure consistent, reliable fluid movement.
  • We test plate movement to validate precise, smooth sample handling.
  • We thoroughly test the prime function of all fluids to ensure the system is clean and functions as designed.
  • We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
  • We test the high-voltage system from minimum to maximum levels to verify consistent, safe operation.

 

What supplies do I need?

You will need the consumables starting out:

 

Can I view the Agilent 5400 Fragment Analyzer in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.

 

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warranted for both parts and labor.
  • The customer should retain all packing materials during the warranty period.

 

What should I watch out for when buying an Agilent 5400 Fragment Analyzer used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

  • We check the power supply to ensure it provides stable output.
  • We test communication with the software to verify seamless interaction and data exchange.
  • We test the initialization and homing processes to ensure accurate and reliable setup.
  • We check pump operation to confirm consistent and reliable fluid movement.
  • We test plate movement to validate precise and smooth sample handling.
  • We thoroughly test the prime function of all fluids to ensure the system is clean and operates as designed.
  • We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
  • We test the high-voltage system from minimum to maximum levels to verify consistent and safe operation.

 

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.

 

Do you have an Agilent 5400 Fragment Analyzer on hand?
  • This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.

 

Here are some of the reasons for our price.

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We have to purchase any missing required accessories to make the instruments complete.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
  • We guarantee that the instrument will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

 

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

How do you pack? Have you packed and shipped Agilent 5400 Fragment Analyzer Instruments in the past? Do you ship Internationally?

Packing

  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

 

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.

 

Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

 

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.