Approved Vendor to many US, Canada, and EU Universities | |
International Shipping | |
Customer References Available | |
Training and Installation Options Available |
- Stock: In Stock
- Brand: Waters
- Product Code: 53531
- Condition: Pre-Owned
For Sale Waters Alliance e2695 Separations Module with 2998 Photodiode Array Detector HPLC System
Pre-owned instrument
- Fully Functional
- Current model
- Serviced & in Stock
- Good cosmetic condition
- Warranty
Here is what you will receive:
- Waters Alliance e2695 Separations Module w/SHC (model # 186269506)
- Waters Alliance Column Heater/Cooler (model #186179100)
- Waters 2998 Photodiode Array Detector (model #186299800) (**New Deuterium Lamp**)
- Solvent Bottle Tray
- Inter-communication cables
- Standard power cords
Please Note: For Computer control and sample analysis, the MassLynx or Empower 3 software and license will need to be purchased from Waters. Otherwise, this instrument can be controlled using that control panel in standalone mode.
Waters Alliance HPLC System Configuration:
e2695 Separations Module with Sample Heating/Cooling:
Solvent management:
- Number of solvents: one to four
- Solvent conditioning: vacuum degas, two operating modes, four chambers, <500 uL internal volume per chamber
- Flow rate range: 0.01 to 10.000 mL/min (0.050 to 5.000 mL/min typical) in 0.001 mL/min increments
- Compressibility compensation: automatic and continuous
- Dwell volume (total system): ≤1.145 mL
- Plunger seal wash: integral, active, programmable
- Gradient profiles: 11 gradient curves (including linear, step [2], concave [4], and convex [4])
- Dry prime/wet prime: automatic front panel control, SystemPREP function for automatic solvent(s) purge
- Flow ramping: time (0.01 to 30.00 min in 0.01 min increments) to reach maximum flow rate
- Maximum operating pressure: 5000 psi (345 bar [0.010 to 3.000 mL/min]) programmable upper and lower limits
- Composition range: 0.0% to 100.0%, in 0.1% increments
- Composition accuracy: ±0.5% absolute, independent of backpressure
- Composition precision: ≤0.15% RSD or ≤0.02 min SD, whichever is greater, based on retention time
- Flow precision: ≤0.075% RSD or ≤0.02 min SD, six replicates, based on retention time or volumetric measures (0.200 to 5.000 mL/min), isocratic premix
- Flow accuracy: ±1% or 10 uL/min, whichever is greater, 0.200 to 5.000 mL/min, (degassed methanol at 600 psi backpressure)
Sample management:
- Number of sample vials: 120 vials, configured in five carousels of 24 vials each
- Number of sample injections: 1 to 99 injections per sample vial
- Sample delivery precision: typically <0.5% RSD, 5 to 80 uL (using standard 250 uL syringe)
- Sample carryover: sample carryover ≤0.0025% for caffeine, under specified conditions; injection needle wash Integral, active, programmable
- Injection accuracy: ±1 uL (±2%) (50 uL, N=6), sample: 100% degassed water, analytical solvent: 100% degassed methanol
- Standard sample vial: 2 mL
- Advanced operations: priority samples, auto additions, auto standards
- Injection volume range: 0.1 to 100.0 uL, standard
- Injector linearity: >0.999 coefficient of deviation (1.000 to 100.000 uL)
- Minimum sample required: 10 uL, using low-volume inserts
Sample temperature control:
- Ambient -25 DegC or 4 DegC (whichever is greater) to 40 DegC in 1 DegC increments
- ±3 DegC temperature accuracy
- 60-min time limit from lab ambient to heating set-point
- 90-min time limit from lab ambient to cooling set-point
Column Heater/Cooler:
- Column environment controllable from 4 DegC up to 65 DegC
- Cooling capability enhances rapid multi-method switching
2998 Photodiode Array Detector:
- Light source: deuterium arc lamp
- Path length: 10 mm (analytical flow cell)
- Cell volume: 8.4 uL (analytical flow cell)
- Pressure limit: 6895 kPa (69 bar, 1000 psi)
- Wetted materials: 316 stainless steel, fused silica
- Wavelength range: 190 to 800 nm
- Bandwidth: 1.2 nm
- Photodiodes: 512
- Digital resolution: 1.2 nm/pixel
- Wavelength accuracy: ±1.0 nm
- Wavelength repeatability: ±0.1 nm
- Linearity: < 5% at 2.0 AU, propylparaben series at 257 nm, analytical flowcell
- Sampling rate: 1, 2, 5, 10, 20, 40, and 80 Hz
Customization/Options:
- Other detectors might be available. Please inquire with our sales team.
Testing performed by our in-house service team:
The Waters Alliance HPLC System passed all tests and was found to be fully functional:
- We inspected and cleaned the system.
- We tested using Empower software.
- We performed the gradient proportioning valve test.
- We ran the seal-washing pump test.
- We tested the pressure limiter.
- We verified the accuracy of the flow rate.
- We conducted a solvent delivery stability test to evaluate the system's ability to deliver solvents consistently.
- We ran gradient tests to ensure consistent and accurate gradient formation.
- We performed the sample cooler set temperature accuracy test to ensure precise temperature control.
- We checked the column oven temperature accuracy to verify its consistency.
- We conducted a repeatability test to confirm the system's reliability.
- We initialized the PDA detector.
- We installed a new lamp.
- We ran tests to verify accurate and reliable detection capabilities.
- We performed calibration verification to ensure measurement accuracy.
Instrument Overview:
- The Alliance HPLC System features integrated solvent and sample management functions that ensure consistent system-to-system performance and high reproducibility.
- A large, intuitive, LCD-based user interface on the e2695 Separations Module allows rapid system set-up through AutoStartPLUS routines that streamline daily startups.
- The 2998 Photodiode Array Detector enables the detection and quantification of multiple components in a sample.
- Improved control of your column environment via a column heater/cooler, allowing you to control temperatures whether you’re using single or multiple columns
- Physical design that provides easy, tool-free access to pistons, seals, and the piston seal-wash as well as the lower needle-wash seal of the sample manager
- The Waters Alliance HPLC System with 2998 Photodiode Array Detector ensures accurate and efficient chromatographic analysis.
Instrument Application:
- Pharmaceutical Quality Control
- Environmental Contaminant Analysis
- Food and Beverage Safety Testing
- Clinical Drug Monitoring
- Forensic Toxicology
The key features:
- High-performance liquid chromatography (HPLC).
- Ultimate chromatographic sensitivity in photodiode array detection with 2998 PDA Detector
- Column heater/cooler for temperature-controlled HPLC separations
- Sample heater/cooler manages labile samples and enhances the stability or solubility of samples.
- Ideal for pharmaceutical, environmental, and food analysis.
Additional technical info:
- Datasheet: Waters Alliance HPLC System Datasheet
- Brochure: Waters Alliance HPLC System Brochure
- Manual: Waters Alliance HPLC System Manual
- Datasheet: Waters 2998 Photodiode Array Detector Datasheet
- Brochure: Waters 2998 Photodiode Array Detector Brochure
- Manual: Waters 2998 Photodiode Array Detector Manual
- Test results: Noise Drift Results
- Test results: Gradient Test Calculations Results
- Test results: Gradient Test CD Results
- Test results: Gradient Test AB Results
- Test results: PDA Calibration Verification Results
- Test results: Repeatability Test Results
Video Overview:
About us
How we pack
Why should you buy from us?
- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping.
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Many upgrade and repair parts are in stock.
- Remote installation and instrument operation training are available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
- It will work!
- Warranty
- An extended warranty may be available.
- You get after-sales technical support.
- Smooth process from the initial quote to order fulfillment.
- Customer references are available.
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*LA* Large Crate ESQ
600#
COMMON QUESTIONS AND ANSWERS BELOW
What steps do you take to verify, service, and confirm the functionality of the Waters Alliance HPLC System?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.
- We inspected and cleaned the system.
- We tested using Empower software.
- We performed the gradient proportioning valve test.
- We ran the seal-washing pump test.
- We tested the pressure limiter.
- We verified the accuracy of the flow rate.
- We conducted a solvent delivery stability test to evaluate the system's ability to deliver solvents consistently.
- We ran gradient tests to ensure consistent and accurate gradient formation.
- We performed the sample cooler set temperature accuracy test to ensure precise temperature control.
- We checked the column oven temperature accuracy to verify its consistency.
- We conducted a repeatability test to confirm the system's reliability.
- We initialized the PDA detector.
- We installed a new lamp.
- We ran tests to verify accurate and reliable detection capabilities.
- We performed calibration verification to ensure measurement accuracy.
What supplies do I need?
You will need the consumables starting out:
Can I view the Waters Alliance HPLC System in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Waters Alliance HPLC System used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We inspected and cleaned the system.
- We tested using Empower software.
- We performed the gradient proportioning valve test.
- We ran the seal-washing pump test.
- We tested the pressure limiter.
- We verified the accuracy of the flow rate.
- We conducted a solvent delivery stability test to evaluate the system's ability to deliver solvents consistently.
- We ran gradient tests to ensure consistent and accurate gradient formation.
- We performed the sample cooler set temperature accuracy test to ensure precise temperature control.
- We checked the column oven temperature accuracy to verify its consistency.
- We conducted a repeatability test to confirm the system's reliability.
- We initialized the PDA detector.
- We installed a new lamp.
- We ran tests to verify accurate and reliable detection capabilities.
- We performed calibration verification to ensure measurement accuracy.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this Waters Alliance HPLC System elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We must purchase any required or missing accessories to complete the instruments.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing and shipping sensitive instruments.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you previously packed and shipped Waters Alliance HPLC System? Do you ship Internationally?
Packing
- Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot, and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.