Thermo Scientific Vanquish Horizon/Flex Quaternary HPLC System with Diode Array, Fluorescence Detectors & Fraction Collector

For Sale Thermo Scientific Vanquish Horizon/Flex Quaternary HPLC System with Diode Array, Fluorescence Detectors & Fraction Collector

• Fully Functional
• Serviced & in Stock
• Current Model
• Good cosmetic condition
• Technical sales consultation available
•  WARRANTY

Estimated pricing new.

VC-D50-A Fluorescence Detector C $ 14,600.00

VF-D11-A Diode Array Detector FG $ 26,600.00

VF-A10-A Split Sampler FT $ 29,000.00
VF-P20-A Quaternary Pump F $ 22,300.00

VH-C10-A Column Compartment H $ 7,190.00

VF-F11-A Integral Fraction Collector FT $ 14,100.00

Here is what you will receive:

• Vanquish Fluorescence Detector C w/ Standard Bio Flow Cell 8uL, 20bar p/n: VC-D50-A
• Vanquish Diode Array Detector FG p/n: VF-D11-A
• Vanquish Split Sampler FT p/n: VF-A10-A
• Vanquish Split Sampler Sample Racks 54 x 12 mm OD vials (≤ 1.5 mL) Qty.(4)
• Vanquish Quaternary Pump F w/ Static Mixer, volume: 350 uL p/n: VF-P20-A
• Vanquish Column Compartment H w/ Active Column Preheater p/n: VH-C10-A
• Fraction collector F p/n: VF-F11-A
• 90 position racks for 8 mL tubes; 13 mm x 100 mm Qty.(2)
• Computer running Chromeleon software ver7.3 (w/ monitor, keyboard, mouse)
• Solvents Rack
• Inter-communication cables
• Standard power cords

Vanquish Horizon/Flex Quaternary HPLC System Configuration:

Vanquish Fluorescence Detector C:

• Optical design: two monochromators with concave holographic gratings and elliptic mirrors for the highest efficiency in light transmission
• Light source: xenon flash lamp (15W)
• Number of photomultipliers: 1
• Wavelength range: Excitation: 200 nm - 630 nm/Emission: 265 nm - 650 nm
• Spectral bandwidth: Excitation: 20 nm/Emission: 20 nm
• Wavelength accuracy: ± 2 nm
• Wavelength repeatability: ± 0.2 nm
• Spectra scanning modes: Single spectrum scan modes: Excitation, Emission, Synchronous
• Number of channels: single channel
• Data collection rate: up to 100 Hz
• Emission filter: fixed filter with 280 nm
• Flow Cell: standard flow cell, biocompatible (8 uL, 2 MPa, fused silica)
• Flow cell thermostat 15 DegC above ambient to 50 DegC absolute

Vanquish Diode Array Detector FG:

• Optical design: single-beam, reverse-optics design with concave holographic grating, achromatic optics, 1024-element photodiode array
• Light source: deuterium lamp, tungsten lamp
• Wavelength range: 190–800 nm (with deuterium and tungsten lamp)
• Spectral bandwidth: pixel resolution: 0.6 nm (average)
• Slit width Settable: wide, narrow
• Wavelength accuracy: ±1 nm
• Wavelength repeatability: ±0.1 nm
• Wavelength calibration: internal calibration with the D-alpha line of the deuterium lamp
• Wavelength validation: internal validation with holmium oxide filter
• Number of signal channels: up to 10 + 3D field
• Data collection rate: up to 250 Hz (including 3D acquisition)
• Noise: <±6 uAU at 254 nm
• Drift: <1 mAU/h (typically <0.5 mAU/h) at 254 nm
• Linearity: <5% at 2.2 AU (typically <5% at 2.7 AU)
• Flow cell: Standard bio, 13 uL Flow cell, path length 10 mm (13 uL, PEEK) 
• Flow cell pressure limit: standard biocompatible flow cell: 5 MPa (50 bar, 720 psi)

Vanquish Split Sampler FT:

• Injection units: 1
• Operating principle: split loop injection
• Pressure range: 2–103 MPa
• Injection volume range: 0.01–25 uL, min. Step = 0.01 uL
• Injection volume accuracy: typically ±0.5% for 10 uL water
• Injection volume precision: <0.25% area RSD for 1 uL (caffeine in water); typically <0.5% area RSD for 0.5 uL (caffeine in water)
• Injection linearity: r > 0.99999 (caffeine in water)
• Injection cycle time: down to 8s depending on separation conditions
• Minimum sample required: 2 uL at 1 uL injection volume
• Carryover (UV): <0.002% with caffeine (typically: <0.0004%)
• Needle wash (external): 1 solvent per injection unit, dip rinse and continuous rinse
• Sample compartment temperature range: 4–40 DegC (≥23 K below ambient at <80% RH)
• Sample temperature accuracy: -2 DegC/+4 DegC
• Sample temperature stability: ±1 DegC
• Dwell volume (contribution of the autosampler to the system gradient delay volume):
• Method Transfer capability: 110 uL with 25 uL sample loop
• Sample capacity: 4 segments useable for sample racks or well plates with SBS footprint format; plus support vial holders with a capacity of 12 x 22.5 mm O.D. vials
• Included sample racks: (4)x54 × 12 mm OD vials (≤1.5 mL)
• Barcode reading: empty segment detection, rack/well plate verification, inventory management

Vanquish Quaternary Pump F:

• Operating Principle: serial dual-piston pump
• Flow Range: 0.001–8 mL/min, in 1 uL/min increments
• Pressure Range: 2–103 MPa; flow rate >5 mL/min, the pressure range decreases 80 MPa
• Compressibility Compensation: fully automated, independent of the composition of the mobile phase
• Flow Accuracy: ±0.1%
• Flow Precision: <0.05% RSD or <0.01 min SD, whichever is greater
• Pulsation Typically: <1.0% or <0.2 MPa, whichever is greater
• Gradient Formation: low-pressure gradient proportioning
• Proportioning Accuracy: ±0.5% of full-scale
• Proportioning Precision: <0.15% SD
• Number of Solvent Lines: 4
• Mixer Volume: 400 uL (50 uL proprietary capillary mixer and 350 uL static mixer)
• Dwell Volume: 679 uL 
• Solvent Degassing: built-in, 4 Channels

Vanquish Column Compartment H with Active Column Preheater:

Operating Principle Column Chamber: Still air and forced air

• Temperature Range: 5–120 DegC in 0.1 DegC increments (18K below ambient)
• Temperature Stability: ±0.05K
• Temperature Accuracy: ±0.5K (up to 80 DegC)
• Heating Performance: <15 min, 20–50 DegC, ±1K Typical: 5 min, 25–40 DegC, ±1K
• Cooling Performance: <15 min, 50–20 DegC, ±1K
• Capacity: 2 column slots

Operating Principle Pre-heater: Active

• Temperature Range: 30–120 DegC  (5K above ambient)
• Temperature Stability: ±0.5K
• Temperature Accuracy: ±2K
• Heating Performance: 80 DegC at 2.5 mL/min, 120 DegC at 1 mL/min (water/acetonitrile (50/50, v/v))
• Capacity: 2 column slots
• Temperature Zones: 1 per device (up to 3 zones in stacked configuration)
• Column ID: 4 column ID tags
• Valve: 2 Positions 6 Ports Thermo Scientific 150MPa bio

Fraction collector F:

• Sample Racks Included: (2)racks for 90 × 13mm (OD) × 100mm (H) tubes (8 mL) 
• Tube Change Time: 0.2 to 0.4 s, depending on collection mode and rack type
• Maximum Tube Height: 150 mm
• Flow Rate Range: 0.4 mm ID drop former: 0.5 – 8 mL/min; 1 mm ID drop former: 8 – 150 mL/min
• Carry-Over Volume: 15 uL

Customization/Options: N/A

Testing performed by our in-house service team:

The Vanquish Horizon/Flex Quaternary HPLC System passed all tests and was found to be fully functional:

Vanquish Quaternary Pump F:

• We performed the initialization test.
• We conducted the self-test.
• We verified the software communication test.
• We performed the gradient proportioning valve test.
• We completed the seal-washing pump test.
• We ran the pressure limiter test.
• We tested the flow rate accuracy.
• We ran a solvent delivery stability.

Vanquish Split Sampler FT:

• We conducted the initialization test.
• We checked the software communication.
• We tested the sample cooler set temperature accuracy.
• We performed the needle wash test.
• We carried out the reproducibility test.

Vanquish Column Compartment H:

• We conducted the initialization test.
• We verified the software communication.
• We carried out the heating/cooling test.
• We performed the switching valve test.
• We tested the active column preheater.
• We checked the leak sensor.

Vanquish Diode Array Detector FG:

• We performed the initialization test.
• We verified the software communication.
• We checked the leak sensor.
• We conducted test injections of the caffeine solution.
• We assessed the UV/VIS lamp lifetime.

Vanquish Fluorescence Detector C:

• We conducted the initialization test.
• We verified the software communication.
• We checked the leak sensor.
• We carried out test injections of the rhodamine solution.
• We checked the lamp's lifetime.

Fraction Collector F:

• We performed the initialization test.
• We verified the software communication.
• We checked the leak sensor.
• We tested the fraction collection accuracy.
• We assessed the tray movement functionality.
• We verified the response of the fraction collector to the stop and start commands from the software.

COMMON QUESTIONS AND ANSWERS BELOW