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Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro

Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

 

  • Stock: In Stock
  • Brand: Roche
  • Product Code: SM100253-480/384
  • Condition: Pre-Owned
$9,995.00
CLICKABLE TABS BELOW

For Sale Roche LightCycler 480-II PCR System with 384 Well Thermocycler Block

 

  • Fully Functional
  • Serviced & in Stock
  •  Technical sales consultation available
  • Includes Continental US 120 Days Warranty & 60 Days International
  •  Extended 1-Year Warranty option available to US customers
  •  Training options available
  •  Preventative Maintenance (PM) & Calibration Performed.

 

Predecessor to: Roche LightCycler Pro


 

Here is what you will receive:

  • Roche PCR Lightcycler 480II
  • 384-S silver thermal cycler block
  • 384-well block cycler cover
  • Computer running LightCycler software
  • Communication and power cables

 

Roche PCR LightCycler 480-II Configuration:

  • Sample capacity: 384-well plate with installed 384-silver block
  • Sample volume: 10-100 ul
  • Run time (96-well plate): < 60 minutes
  • Excitation: 5 excitation filters, high-intensity broad-spectrum xenon lamp
  • Detection: 6 detection filters, signals detected online by CCD camera
  • Temperature control: Peltier-based heating and cooling
  • Temp Range: 37 - 95DegC
  • Dimensions (WxDxH): 60 x 60 x 54.5 cm. | 24 x 24 x 21.5 in.
  • Weight: 55 kg | 121 lbs


Excitation:

  • Type: xenon reflector lamp
  • Luminous intensity: 10 uW/mm2
  • Wattage: 100 W
  • Lifetime: > 500 h


Detector:

  • Type: cooled monochrome CCD camera.
  • Resolution: 1024 x 1344 pixel
  • Integration time: 10 ms to 10 s
  • Integration time selection: dynamic or manual
  • Sensitivity: < 0.2 nmol/l fluorescein, typically 0.1 nmol/l (20ul reaction volume)
  • Reproducibility: CV =< 0.15%
  • Crosstalk well-to-well: < 0.2% optically; < 0.02% with software correction


Filter Set of the LightCycler 480 II Instrument:

Excitation wavelengths (nm):

  • Bandpass / Half Band Width (HBW)
  • 440nm / 35nm
  • 465nm / 25nm
  • 498nm / 40nm
  • 533nm / 25nm
  • 618nm / 35nm


Detection wavelengths (nm):

  • Bandpass / Half Band Width (HBW)
  • 488nm / 20nm
  • 510nm / 20nm
  • 580nm / 20nm
  • 610nm / 20nm
  • 640nm / 20nm
  • 660nm / 95nm(low pass)

 

96-well Silver Thermal Block:

  • Temperature control: Peltier-based heating and cooling
  • Temperature range: 37-95DegC
  • Heating rate 96-well block: 4.4DegC/s
  • Cooling rate 96-well block: 2.2DegC/s

 

Customization/Options:

96 block options are available and easily exchangeable. Please inquire with our sales team.

 

Testing performed by our in-house service team:

The Roche PCR LightCycler 480-II passed all tests and was found to be fully functional:

  • The Cobas system components are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary.
  • The proper operation of electronic components is confirmed
  • We perform stress tests on the thermal block and electronics. 
  • We perform tests of all the instrument functions listed below
  • We inspect the optics.
  • We perform measurement excitation.
  • We assess the operation of the filter wheel.
  • We check filter dark position.
  • We verify the motor connection.
  • We calibrate flat field parameters.
  • We confirm the alignment.
  • We check hot pixels, bias value, and noise
  • We inspect the camera.
  • We check the cover heater temperature.
  • We verify X and Y-axis calibration.
  • We confirm excitation measurement and detector alignment.
  • We test barcode scanner functionality.
  • We verify the barcode reader’s operation.

 

Instrument Overview: 

  • The Roche PCR LightCycler 480-II is a high-throughput real-time PCR system.
  • It offers precise and reliable results with excellent sensitivity and specificity.
  • This instrument is designed for research laboratories and diagnostic settings.
  • The LightCycler 480-II allows for multiplexing and high sample throughput.
  • It features advanced software for data analysis and result interpretation.
  • The Roche PCR LightCycler 480-II enhances workflow efficiency with its user-friendly interface.  

 

Instrument Application:

  • For Life Science Research
  • For General Laboratory Use
  • Sequence-Independent Detection Assays
  • Sequence-Specific Probe Binding Assays
  • Single-labeled probes (SimpleProbe chemistry)
  • Hybridization probes (HybProbe chemistry)
  • Hydrolysis probes (5 ́-nuclease assay)

 

The key features of Roche PCR LightCycler 480-II:

  • Advanced PCR technology
  • High-throughput analysis
  • Intuitive software interface
  • Precise temperature control
  • Versatile applications
  • Reliable performance

 

Additional technical info:

 

Video Overview:

 

About us

 

How we pack

 

Why should you buy from us?

 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping.
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Many upgrade and repair parts are in stock.
  • Remote installation and instrument operation training are available.
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 

  • It will work!
  • Warranty
  • An extended warranty may be available.
  • You get after-sales technical support.
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

COMMON QUESTIONS AND ANSWERS BELOW

 

Steps you take to verify, service, and confirm the functionality of the Roche LightCycler 480-II?

Summary of testing & maintenance we perform (depending on included configuration):

  • We inspect the Roche LightCycler 480-II for physical damage.
  • The LightCycler 480-II system components are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary.
  • The proper operation of electronic components is confirmed
  • Necessary repairs and services performed to make sure the LightCycler 480-II will function as intended
  • We perform stress tests on the thermal block and electronics. 
  • We perform tests of all the instrument functions listed below
    • Self-check: PASSED
    • Check Lamp Stability: PASSED
    • Check Camera and Filter: PASSED
    • Perform Measurement Excitation testing: PASSED
    • Check Filter Wheel Response Time: PASSED
    • Check Filter Dark Position: PASSED
    • Check Filter Motor Connection: PASSED
    • Check Flat Field Parameters: PASSED
    • Check Cover Marker Coordinates: PASSED
    • Check Camera Hot Pixels: PASSED
    • Check Bias Value: PASSED
    • Check Noise: PASSED
    • Exchange Reference Channel: PASSED
    • Check Camera: PASSED
    • Check Cover Heater Temperature: PASSED
    • Tested barcode scanner: PASSED
    • X and Y-axis calibration: PASSED (unit is less than 5% of allowable offset)
    • Excitation Measurement and Detector alignment: PASSED
    • Blank plate run performed with no errors: PASSED

What supplies do I need?

You will need the consumables starting out: 

 

Can I view the Roche LightCycler 480-II in person at your facility or do a live Zoom demo before it is shipped to me?

We offer both onsite and remote demos via Zoom.

  • We invite you to come in for an onsite Demo or schedule a Zoom demo of the LightCycler 480-II.

 What about the warranty? Installation, training, maintenance?

Roche LightCycler 480-II Advanced PCR System comes with a 90-day Warranty.

 

Warranty Terms:

  • The warranty term is 90 Days from the date of the Roche LightCycler 480-II’s arrival at the destination.
  • This includes parts and labor.
  • The customer should retain all packing materials during the warranty period. 

 

Exceptions: 

  • Damage to the system due to the use of incorrect consumables.
  • Electrical damage from plugging into an incorrect voltage. 
  • Improper storage or transportation procedures.
  • Issues caused by lack of proper standard maintenance.
  • Moisture/Water damage.
  • Physical damage.

 

Optional Training Package:

We can offer Roche Instrument Training (Remote setup, general maintenance procedures guidance). This service is highly recommended to avoid a lot of the above issues. 

 

What should I watch out for when buying a Roche LightCycler 480-II system used/pre-owned from another lab?

Basic steps to confirm quality:

  • Confirm that all the Roche LightCycler 480-II covers are not cracked or broken. (It is very common for the Thermal Block door to be damaged, affecting the interlocks.)
  • Confirm the plate drawer opens and closes smoothly.
  • Confirm that the instrument is on a Windows 10 computer and communicates. 
  • Make sure the Thermal Block is in good working order. (They are thousands of dollars to replace.)  
  • Confirm that the Lamp is in good condition and the Xenon reflector lamp is new.

 

If you have concerns, we highly recommend inspecting the Roche LightCycler 480-II  in person or doing a live Zoom call; this will allow you to evaluate the condition.

 

 Do you have Roche LightCycler 480-II units on hand?
  • We stock multiple Roche LightCycler 480-II  systems along with many replacement parts to back our warranty.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get the Roche LightCycler 480-II  into your lab faster.
  • POs are accepted from US and Canadian universities.

 

 How fast will you ship?
  • We can ship within a few days to 2 weeks. Ship times depend on the current demand for our instruments. Members of our sales team will be able to offer current estimates.

 

Will my instrument be insured during shipping?
  • All Roche LightCycler 480-II are shipped fully insured within the US and internationally as long as we are a shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

 How do you pack? Have you packed and shipped LightCyclers in the past? Do you ship Internationally?

Packing

  • Most Roche LightCycler 480-II systems ship in fully enclosed ISPM-15 certified wooden crates, with proper padding underneath and the sides, and have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally we will only ship via Air Freight or Air Parcel (such as UPS, DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience shipping robotic liquid handelers around the world.

Our experience and process

  • We have safely packed and shipped hundreds of sensitive analytical instruments, including Roche LightCycler 480-II.
  • Before final packing/shipping, the Roche LightCycler 480-II is re-checked to verify performance.

 

*SM* Small Crate
275#

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is certainly worth it, and here is why.  Feel free to ask for feedback from hundreds of customers who bought from us.

  • You will save around 55-70% vs. buying a new Roche.  Why not spend leftover funds on another instrument for your lab?
  • You will get a 90-day warranty, plus, when available, an option to obtain an extended warranty agreement. 
  • Get peace of mind knowing that your instrument was tested and working the day it left our facility and is covered if there are faults.
  • Many customers choose to invest funds into Extended Warranties or maintenance plans for additional piece of mind.

 

The Roche LightCycler 480-II could be priced up to $65,000. So why not save your funds by buying Roche LightCycler 480-II used, get the high-quality sample preparation you expect from Roche, and use your leftover funds elsewhere?

 Sustainability
  • Well, this one is easy; buying a pre-owned Roche LightCycler 480-II is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.   

 I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-366-2699) or complete this form to get your questions answered or receive a formal Quote.

 

 

We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend thousands of dollars and receive a damaged instrument.

Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing.

We generally ship parcels either via UPS or freight for all larger goods.

Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant).

On freight shipments, we use 2” 1.7 lb. density foam, which provides excellent cushioning and support for the instrument.

Instruments are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provides cushioning in all directions and holds instruments firmly in place.

Final step is to photograph everything inside the crate and then seal it with screws.

 

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies