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Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block

Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

  • Stock: In Stock
  • Brand: Roche
  • Product Code: 51601
  • Condition: Pre-Owned
$11,995.00
CLICKABLE TABS BELOW

For Sale Roche Cobas Z PCR LightCycler 480-II w/ Thermocycler 96-S block


Pre-owned instrument

  • Fully Functional
  • Current model
  • Serviced & in Stock
  • Good cosmetic condition
  • Warranty
  • An extended warranty option and training may be available.

 

The Roche Cobas Z 480 is the IVD version of LightCycler 480 II
They are identical aside from Cobas Z having a higher level of certification than Lightcyler 480 II

 

Here is what you will receive:

  • Roche Cobas Z480 PCR Lightcycler
  • 96-S silver thermal cycler block
  • 96-well block cycler cover
  • Computer running LightCycler software v1.5
  • Communication and power cables

 

Roche Cobas Z PCR LightCycler 480-II Configuration:

  • Sample capacity: 96-well plate with installed 96-silver block
  • Sample volume: 10-100 ul
  • Run time (96-well plate): < 60 minutes
  • Excitation: 5 excitation filters, high-intensity broad-spectrum xenon lamp
  • Detection: 6 detection filters, signals detected online by CCD camera
  • Temperature control: Peltier-based heating and cooling
  • Temp Range: 37 - 95DegC
  • Dimensions (WxDxH): 60 x 60 x 54.5 cm. | 24 x 24 x 21.5 in.
  • Weight: 55 kg | 121 lbs


Excitation:

  • Type: xenon reflector lamp
  • Luminous intensity: 10 uW/mm2
  • Wattage: 100 W
  • Lifetime: > 500 h


Detector:

  • Type: cooled monochrome CCD camera.
  • Resolution: 1024 x 1344 pixel
  • Integration time: 10 ms to 10 s
  • Integration time selection: dynamic or manual
  • Sensitivity: < 0.2 nmol/l fluorescein, typically 0.1 nmol/l (20ul reaction volume)
  • Reproducibility: CV =< 0.15%
  • Crosstalk well-to-well: < 0.2% optically; < 0.02% with software correction


Filter Set of the LightCycler 480 II Instrument:

Excitation wavelengths (nm):

  • Bandpass / Half Band Width (HBW)
  • 440nm / 35nm
  • 465nm / 25nm
  • 498nm / 40nm
  • 533nm / 25nm
  • 618nm / 35nm


Detection wavelengths (nm):

  • Bandpass / Half Band Width (HBW)
  • 488nm / 20nm
  • 510nm / 20nm
  • 580nm / 20nm
  • 610nm / 20nm
  • 640nm / 20nm
  • 660nm / 95nm(low pass)

 

96-well Silver Thermal Block:

  • Temperature control: Peltier-based heating and cooling
  • Temperature range: 37-95DegC
  • Heating rate 96-well block: 4.4DegC/s
  • Cooling rate 96-well block: 2.2DegC/s

 

Customization/Options:

384 block options are available and easily exchangeable. Please inquire with our sales team.

 

Testing performed by our in-house service team:

  • The Cobas system components are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary.
  • The proper operation of electronic components is confirmed
  • We perform stress tests on the thermal block and electronics. 
  • We perform tests of all the instrument functions listed below
  • We inspect the optics.
  • We perform measurement excitation.
  • We assess the operation of the filter wheel.
  • We check filter dark position.
  • We verify the motor connection.
  • We calibrate flat field parameters.
  • We confirm the alignment.
  • We check hot pixels, bias value, and noise
  • We inspect the camera.
  • We check the cover heater temperature.
  • We verify X and Y-axis calibration.
  • We confirm excitation measurement and detector alignment.
  • We test barcode scanner functionality.
  • We verify the barcode reader’s operation.

 

Instrument Overview: 

  • The Roche Cobas Z PCR LightCycler 480-II is a high-throughput real-time PCR system.
  • It offers precise and reliable results with excellent sensitivity and specificity.
  • This instrument is designed for research laboratories and diagnostic settings.
  • The LightCycler 480-II allows for multiplexing and high sample throughput.
  • It features advanced software for data analysis and result interpretation.
  • The Roche Cobas Z PCR LightCycler 480-II enhances workflow efficiency with its user-friendly interface.  

 

Instrument Application:

  • For Life Science Research
  • For General Laboratory Use
  • Sequence-Independent Detection Assays
  • Sequence-Specific Probe Binding Assays
  • Single-labeled probes (SimpleProbe chemistry)
  • Hybridization probes (HybProbe chemistry)
  • Hydrolysis probes (5 ́-nuclease assay)

 

The key features of Roche Cobas Z PCR LightCycler 480-II:

  • Advanced PCR technology
  • High-throughput analysis
  • Intuitive software interface
  • Precise temperature control
  • Versatile applications
  • Reliable performance

 

Additional technical info:

 

Video Overview:

 

About us

 

How we pack

 

Why should you buy from us?

 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping.
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Many upgrade and repair parts are in stock.
  • Remote installation and instrument operation training are available.
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 

  • It will work!
  • Warranty
  • An extended warranty may be available.
  • You get after-sales technical support.
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*SM* Small Crate
275#

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What steps do you take to verify, service, and confirm the functionality of the Roche Cobas Z PCR LightCycler 480-II?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

 

  • The Cobas system components are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary.
  • The proper operation of electronic components is confirmed
  • We perform stress tests on the thermal block and electronics. 
  • We perform tests of all the instrument functions listed below
  • We inspect the optics.
  • We perform measurement excitation.
  • We assess the operation of the filter wheel.
  • We check filter dark position.
  • We verify the motor connection.
  • We calibrate flat field parameters.
  • We confirm the alignment.
  • We check hot pixels, bias value, and noise
  • We inspect the camera.
  • We check the cover heater temperature.
  • We verify X and Y-axis calibration.
  • We confirm excitation measurement and detector alignment.
  • We test barcode scanner functionality.
  • We verify the barcode reader’s operation.

 

What supplies do I need?

You will need the consumables starting out:

 

Can I view the Roche Cobas Z PCR LightCycler 480-II in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.

 

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warrantied for both parts and labor.
  • The customer should retain all packing materials during the warranty period.

 

Installation & Training:

Option 1 – In Person

If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.

 

Option 2 – Remote/Virtual

Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.

 

Both training options include:

  • Review of the instrument and its capabilities.
  • General instrument and software operation.
  • Review of the recommended maintenance procedures.

 

We generally recommend these training services if you never worked with this instrument.

 

What should I watch out for when buying a Roche Cobas Z PCR LightCycler 480-II used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

 

  • The Cobas system components are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary.
  • The proper operation of electronic components is confirmed
  • We perform stress tests on the thermal block and electronics. 
  • We perform tests of all the instrument functions listed below
  • We inspect the optics.
  • We perform measurement excitation.
  • We assess the operation of the filter wheel.
  • We check filter dark position.
  • We verify the motor connection.
  • We calibrate flat field parameters.
  • We confirm the alignment.
  • We check hot pixels, bias value, and noise
  • We inspect the camera.
  • We check the cover heater temperature.
  • We verify X and Y-axis calibration.
  • We confirm excitation measurement and detector alignment.
  • We test barcode scanner functionality.
  • We verify the barcode reader’s operation.

 

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.

 

Do you have a Roche Cobas Z PCR LightCycler 480-II on hand?
  • This is a common Roche Cobas Z PCR LightCycler 480-II, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this Roche Cobas Z PCR LightCycler 480-II instrument elsewhere, possibly for less.

 

Here are some of the reasons for our price.

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We must purchase any required or missing accessories to complete the instruments.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing and shipping sensitive instruments.
  • We guarantee that the instrument will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

 

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

How do you pack? Have you previously packed and shipped Roche Cobas Z PCR LightCycler 480-II? Do you ship Internationally?

Packing

  • Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

 

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

 

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

 

Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies