Leica DM6000 Upright Fluorescence Motorized Microscope with Motorized XY Stage (New Filters) Pred DM6

Customer: Hvidovre Hospital, Denmark

SKU-32263
 

Customer Application: 

This customer was looking for a fully motorized fluorescence imaging microscope to view their cancer research. The Leica DM6000 is a modular microscope system, that allows customization and flexibility to meet the specific needs of cancer research.

Customer Requested Modifications:

• From a full spectrum metal halide fluorescence source to a multi-band LED light source. LED light sources emit less heat and negligible amounts of UV or infrared radiation, minimizing phototoxic effects on live cancer cells during imaging. This is especially advantageous for long-term imaging studies or investigations involving sensitive cellular processes or drug response evaluations.
• From our default DAPI, FITC, Texas Red filter sets to Attenuated DAPI, FITC, Texas Red, and CY3 filters. The Leica DM6000 can be equipped with fluorescence modules, such as fluorescence filter sets and fluorescence illuminators, allowing for precise fluorescence imaging and analysis of cancer cells and fluorescently tagged biomarkers.
• The total magnification range of this microscope with 10x eyepieces and installed objectives was increased to 400x. The Leica DM6000 is known for its exceptional optical performance, providing high-resolution and contrast imaging. Its high-quality lenses and optics ensure clear visualization of cellular structures, which is crucial for studying cancer cells and their characteristics.
• The stage was swapped from a manual XY stage to a motorized stage. A precise and stable stage with fine focus adjustment allows for accurate positioning and focusing of the slide containing cancer cells.
• In combination with the base’s motorized Z focus, the new stage allowed for imaging options like Z stacking with mosaic stitching. The Leica DM6000 offers imaging automation capabilities, such as motorized stages, autofocus systems, and advanced software, enabling efficient and precise imaging of multiple samples, time-lapse experiments, and image analysis workflows.

Customer: Weill Cornell Medicine, New York

SKU-25856

Customer Application: 

The customer was looking for a way to scan a single slide for fluorescence immunology samples and requested phase contrast options. Fluorescence microscopy enables the detection and analysis of immune responses, including immune cell interactions, antibody labeling, and cytokine expression within tissues.

Customer Requested Modifications:

• From our default, DAPI, FITC, Texas Red filter sets to FITC, Texas Red, and CY3 filtersImmunology often involves the use of fluorescent labeling to visualize specific immune cells, antibodies, or molecular markers. The Leica DM6000 can be equipped with fluorescence modules and filter sets, allowing precise and high-quality fluorescence imaging for immunological studies. 
• Phase contrast annuals and matching objectives were added to allow for contrast imaging. Immunology studies often require visualization of cellular morphology and structural details. The Leica DM6000 supports various contrast techniques, including brightfield, phase contrast, and differential interference contrast (DIC), enabling researchers to observe immune cells and their interactions with enhanced contrast and detail. 
• The total magnification range of this microscope with 10x eyepieces and installed objectives was increased to 630x. The Leica DM6000 delivers exceptional optical performance, including high-resolution imaging capabilities. This is critical for immunology research, as it allows researchers to observe and analyze fine details of immune cells, subcellular structures, or cellular interactions.
• The CMOS camera was upgraded to a CCD which delivers exceptional optical performance, including high-resolution imaging. This is critical for immunology research, as it allows researchers to observe and analyze fine details of immune cells, subcellular structures, or cellular interactions.
• The new stage allowed for full slide scanning using image capture and processing software. These automation capabilities enhance workflow efficiency and enable high-throughput imaging and data acquisition.  

Customer: International Independent Researcher

SKU-24565

Customer Application: 

This customer was looking for a reasonably priced time-lapse slide-imaging microscope for biological studies with DIC options for Pharmacological research. The Leica DM6000 microscope provides valuable functionality and performance for pharmacology and drug discovery research. It empowers researchers to visualize, analyze, and understand the effects of drugs on cellular processes, contributing to the development of new therapeutics and advancements in pharmacological science.

Customer Requested Modifications:

• From our default, DAPI, FITC, Texas Red filter sets to DAPI, FITC, Texas Red, and CY3 filters. Pharmacology and drug discovery often involve the use of fluorescent probes, dyes, or markers to study drug-target interactions, cellular responses, and drug efficacy. The Leica DM6000 can be equipped with fluorescence modules and filter sets, enabling precise and high-quality fluorescence imaging for pharmacological studies. 
• Differential Interference Contrast prisms and matching objectives were added to allow for contrast imaging. The Leica DM6000 supports various contrast techniques, such as brightfield, phase contrast, and differential interference contrast (DIC). These techniques facilitate the observation of cellular morphology, structural changes, and drug-induced alterations, enhancing the visualization of cellular responses to pharmacological treatments. 
• The total magnification range of this microscope with 10x eyepieces and installed objectives was increased to 400x. The Leica DM6000 delivers exceptional optical performance, providing high-resolution imaging capabilities. This is crucial in pharmacology and drug discovery research to observe and analyze cellular structures, drug uptake, intracellular localization, and drug-target interactions at a microscopic level.
• The stage was swapped from a manual XY stage to a motorized stage. Pharmacological studies often require imaging multiple samples or conducting high-throughput experiments. The Leica DM6000 features motorized stage and imaging automation capabilities, enabling efficient and precise sample positioning, multi-site imaging, and time-lapse experiments. Automation reduces human error and enhances workflow efficiency.
• The head was swapped for a more ergonomic model. Prolonged microscope use is common in pharmacology research. The Leica DM6000 is designed with ergonomic considerations, such as adjustable eyepieces, comfortable stage controls, and customizable settings, ensuring user comfort during extended periods of observation, analysis, and experimentation. 

Customer: Research Facility in Shanghai China 

SKU-30483
Customer Application: 

This customer was looking for a budget-friendly motorized microscope for viewing cells for neurological research. In neuroscience, fluorescence microscopy helps researchers visualize neuronal structures, track neural activity, study synaptic connections, and investigate the mechanisms underlying neurological disorders.

Customer Requested Modifications:

• Our default DAPI, FITC, and Texas Red filter sets were changed to Texas Red, CY3, and CY5. Neurological studies often require the simultaneous imaging of multiple fluorophores or fluorescent labels to investigate complex neuronal processes or multiple molecular targets. The Leica DM6000 supports multi-channel imaging, allowing researchers to capture and analyze multiple fluorescent signals within a single sample.
• The total magnification range of this microscope with 10x eyepieces and installed objectives was increased to 630x. The Leica DM6000 provides excellent optical performance, including high-resolution imaging capabilities. This is crucial in neurology research to observe and analyze fine details of neuronal structures, synaptic connections, and cellular morphology.
• The addition of Micromanager and FIJI software allowed for quantitative analysis of neuronal structures, such as dendritic spine density, axonal length, or neuronal connectivity. The Leica DM6000 combined with imaging software for automated image analysis, data quantification, and statistical analysis, facilitating accurate and efficient data processing.
• The stage was swapped from a manual XY stage to a motorized stage. The Leica DM6000 offers imaging automation features, including motorized stages, which are valuable for high-throughput imaging, multi-site imaging, and time-lapse experiments in neurology research. Automation enhances workflow efficiency and allows for precise and reproducible imaging.
• The new stage allowed for joystick control which allowed for quick scanning of their samples during viewing. Neurology research can involve long hours of microscope use. The Leica DM6000 is designed with ergonomic considerations, such as adjustable eyepieces, comfortable stage controls, and customizable settings, ensuring user comfort during extended periods of observation and analysis.

Customer: Baylor University, Texas

SKU-26413

Customer Application: 

This customer requested a fully motorized microscope that could be used for studying the development of organisms by visualizing morphological changes, tracking cell migration, and examining gene expression patterns during embryogenesis.

Customer Requested Modifications: 

• From our default, DAPI, FITC, Texas Red filter sets to DAPI, FITC, Texas Red, and CY3 filters. Developmental biology often involves the use of fluorescent markers or dyes to visualize specific developmental processes, gene expression patterns, or cell lineage tracing. The Leica DM6000 can be equipped with fluorescence modules and filter sets, enabling precise and high-quality fluorescence imaging for studying cellular dynamics and molecular interactions during development. 
• The total magnification range of this microscope with 10x eyepieces and installed objectives was increased to 400x. The Leica DM6000 provides exceptional optical performance, including high-resolution imaging capabilities. This is crucial in developmental biology to observe and analyze fine details of embryonic structures, tissue morphogenesis, and cellular differentiation during various stages of development.
• The stage was swapped from a manual XY stage to a motorized stage. The Leica DM6000 offers imaging automation features, including motorized stages and autofocus systems, which are valuable for high-throughput imaging, time-lapse experiments, and multi-site imaging in developmental biology research. Automation enhances workflow efficiency and allows for precise and reproducible imaging.
• The new stage allowed for a full slide scanning function to be added to time-lapse image capture. Utilizing Micromanager and FIJI software, quantitative analysis of developmental structures, such as cell counts, tissue measurements, or growth dynamics is possible. The Leica DM6000 combined with imaging software for automated image analysis, data quantification, and statistical analysis, facilitating accurate and efficient data processing.

Customer: Doyle Lab, Arizona 

SKU-15307

Customer Application: 

This customer wanted an upright microscope for viewing samples at very high magnification in molecular biological studies. The Leica DM6000 microscope provides researchers in molecular biology with the necessary tools and performance to investigate molecular interactions, cellular processes, and molecular mechanisms. It aids in advancing our understanding of gene expression, molecular signaling pathways, and molecular interactions, contributing to the field of molecular biology. 

Customer Requested Modifications:

• From our default, DAPI, FITC, Texas Red filter sets to DAPI, Texas Red, and CY3 filters. Molecular biology often involves the use of fluorescent probes, dyes, or markers to visualize specific molecules, gene expression patterns, or cellular events. The Leica DM6000 can be equipped with fluorescence modules and filter sets, enabling precise and high-quality fluorescence imaging for studying molecular interactions, protein localization, and cellular processes. 
• The total magnification range of this microscope with 10x eyepieces and installed objectives was increased to 1000x. The Leica DM6000 provides exceptional optical performance, including high-resolution imaging capabilities. This is crucial in molecular biology to visualize and analyze subcellular structures, cellular processes, and molecular interactions at a microscopic level.
• APO objectives were selected for their superior imaging capabilities.APO objectives are designed to minimize chromatic aberration, which is the phenomenon that causes different colors of light to focus at slightly different points. This reduction in chromatic aberration improves image quality by enhancing color accuracy, contrast, and resolution. It allows for clearer and more precise imaging of samples, especially when using multiple wavelengths or fluorescence imaging.
• The stage was swapped from a manual XY stage to a motorized stage. The Leica DM6000 offers imaging automation features, including motorized stages and autofocus systems, which are valuable for high-throughput imaging, time-lapse experiments, and multi-site imaging in molecular biology research. Automation enhances workflow efficiency and allows for precise and reproducible imaging. The new stage allowed for the still placement of the samples during oil immersion image capture
• The addition of Micromanager and FIJI software allows for quantitative analysis of molecular structures, protein expression levels, or cellular dynamics. The Leica DM6000 can be combined with imaging software for automated image analysis, data quantification, and statistical analysis, facilitating accurate and efficient data processing.