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We apologize this Instrument is currently | |
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![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | US & International Shipping |
- Stock: SOLD
- Brand: Cytiva
- Product Code: 67282
- Condition: Pre-Owned
For Sale Cytiva Akta Avant 25 Chromatography System
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- WARRANTY
- Includes remote instrument setup and training session
Here is what you will receive:
- Cytiva AKTA Avant 25 Chromatography System
- Computer with Unicorn software v.7.8
- Fraction collector cassette tray (Two cassettes included, please inquire for available inventory)
- 2 x Column holder
- 2 x Tubing holder spool
- Communication and power cables
Akta Avant 25 Chromatography System Configuration (Installed modules):
- Sample inlet valve 2nd (V9-S2)
- Versatile valve (V9-V)
- Versatile valve 2 (V9-V)
- Versatile valve 3 (V9-V)
- System pump A (P9-A)
- System pump B (P9-B)
- Sample pump (P9-S)
- Mixer (M9)
- Inlet valve A (V9-IA)
- Inlet valve B (V9-IB)
- Sample inlet valve (V9-IS)
- Quaternary valve (Q9) (Standalone – not mounted)
- Injection valve (V9-Inj)
- Column valve (V9-C)
- pH valve (V9-pH)
- Outlet valve (V9-O)
- UV monitor (U9-M)
- Conductivity monitor (C9)
- System pressure monitor (R9)
- Sample pressure monitor (R9)
- Fraction collector
- Sample loop volume: 2ml
Customization/Options: N/A
Testing performed by our in-house service team:
The Akta Avant 25 Chromatography System passed all tests and was found to be fully functional:
- We performed power-up and initialization testing.
- We verified software functionality and PC connectivity.
- We conducted pump priming and confirmed proper operation.
- We evaluated the performance of the UV, conductivity, and pH monitors.
- We tested the fraction collector rack code reader and confirmed correct identification.
- We performed the fraction collector dispensing test and verified accurate collection.
- We assessed system flow rate accuracy and confirmed compliance with specifications.
- We evaluated the sample pump flow rate accuracy and confirmed stable delivery.
- We tested system gradient formation and confirmed proper gradient performance.
- We performed the air sensor test and confirmed proper detection.
Instrument Overview:
- Cytiva Akta Avant 25 Chromatography System is built as a highly modular, automated liquid chromatography platform.
- Architecture combines multiple valve clusters, enabling flexible routing, switching, and multi-buffer handling.
- Dual system pumps with a dedicated sample pump provide controlled, scalable, and reproducible flow delivery.
- Integrated dynamic mixing and valve coordination support precise gradient generation and method flexibility.
- Embedded monitoring of UV, conductivity, and pressure ensures continuous, real-time process supervision.
- Fraction handling and injection subsystem enable automated sample introduction and collection with consistent performance.
Instrument Application:
- Protein purification (e.g., antibodies, enzymes)
- Preparative chromatography for biomolecule isolation
- Process development and scale-up studies
- Buffer screening and method optimization
- Quality control and analytical verification of samples
The Key Features:
- Modular platform for automated chromatography
- Multiple valves enable flexible fluid routing
- Dual system pumps + sample pump ensure control
- Integrated mixer supports precise gradient formation
- UV, conductivity, and pressure monitoring built in
- Fraction collector enables automated collection
Additional technical info:
Video Overview:
About us

How we pack

VIEW SOME OF THE CUSTOMERS WE HELPED HERE
Why do so many customers choose to order from us?

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*LA* Large Crate
675#
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Akta Avant 25 Chromatography System?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.
- We perform power-up and initialization testing.
- We verify software functionality and PC connectivity.
- We conduct pump priming to confirm proper operation.
- We evaluate UV, conductivity, and pH monitor performance.
- We test the fraction collector rack code reader for correct identification.
- We perform the fraction collector dispensing test to verify accurate collection.
- We assess system flow rate accuracy.
- We evaluate sample pump flow rate accuracy to confirm stable delivery.
- We test system gradient formation and confirm proper gradient performance.
- We perform the air sensor test to confirm proper detection.
What supplies do I need?
You will need the consumables starting out:
- Chromatography columns (e.g., affinity, ion exchange, SEC)
- Prepacked media/resins (agarose, silica, polymer-based)
- Buffers and buffer components (salts, acids, bases, reagents)
- Sample filters and syringe filters (0.22 µm / 0.45 µm)
- Tubing, fittings, and connectors (biocompatible materials)
- Sample loops (various volumes)
- Vials, bottles, and reservoir containers
- pH calibration solutions and conductivity standards
- Cleaning and sanitization solutions (e.g., NaOH, ethanol)
- Waste containers and collection tubes for fractions
Can I view the Akta Avant 25 Chromatography System in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying an Akta Avant 25 Chromatography System used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We perform power-up and initialization testing.
- We verify software functionality and PC connectivity.
- We conduct pump priming to confirm proper operation.
- We evaluate UV, conductivity, and pH monitor performance.
- We test the fraction collector rack code reader for correct identification.
- We perform the fraction collector dispensing test to verify accurate collection.
- We assess system flow rate accuracy.
- We evaluate sample pump flow rate accuracy to confirm stable delivery.
- We test system gradient formation and confirm proper gradient performance.
- We perform the air sensor test to confirm proper detection.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have an Akta Avant 25 Chromatography System on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Akta Avant 25 Chromatography System Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.
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