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BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS - AV

BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS - AV
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS - AV
  • Stock: In Stock
  • Brand: BD
  • Product Code: 50165
  • Condition: Pre-Owned
$52,995.00
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Here is a BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors with HTS

Predecessor to:
Current model

Condition:
This BD LSRFortessa Cell Analyzer Flow Cytometer is in good working condition.

Please see the exact video and photos below.

Testing we performed:
The BD LSRFortessa Cell Analyzer Flow Cytometer passed all tests and was found to be fully functional:
Connected to software (Diva 9): PASSED
Ran fluidics priming and sequences: PASSED
Ran several cleaning cycles (long clean, flow cell clean, etc.): PASSED
Tested HTS functionality: PASSED
Performed Optical Alignment: PASSED
(Homing, Priming, Cleaning): PASSED
Tested run with 8 Peak Beads: PASSED

System consists of:
BD LSRFortessa Flow Cytometer
HTS High Throughput Sampler
Computer with FACSDiva software ver9.0 w/ Dongle (with monitor, keyboard, mouse)
Communication and power cables.

Configuration and Setup:
Optics:
(4) Lasers: Violet (405nm), Blue (488nm), Yellow/ Green (561nm), Red (650nm)
(16) Colors simultaneously
(18) Detector Channels
- Forward Scatter Detection: Photodiode detector with a 488/10 BP filter
- Side Scatter Detector: Photomultiplier tube (PMT) with a 488/10 BP filter
- 405 violet laser detection channels: A (780/60, 750LP), B (710/50, 690LP), C (670/30, 635LP), D (610/20, 600LP), E (525/50, 505LP) F (450/50, GG420)
- 488 blue laser detection channels: A (710/50, 685LP), B (530/30, 505LP)
- 561 yelllow/green laser detection channels: A (780/40, 750LP), B (710/50, 685LP), C (670/30, 635LP), D (610/20, 600LP), E (586/15)
- red laser detection channels: A (780/60, 750LP) B (730/45, 690LP), C (670/30, RG665)

Fluorescence Sensitivity:
- FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
- PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
- PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5)
- APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
Fluorescence Resolution: Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
Fluorescence Linearity: Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
Data Acquisition Rate: 40,000 events/sec with beads

Fluidics:
Sample Flow Rates: Front button panel provides three modes: RUN, STANDBY, and PRIME
Continuously adjustable flow rate, plus three preset flow rates: LO: 12 uL/min; MED: 35 uL/min; HI: 60 uL/min

Dimensions (H x W x D): 76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.)
Weight: ~159 kg (~350 lb)

High Throughput Sampler:
The BD High Throughput Sampler (HTS) is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate.
HTS Throughput Acquisition: less than 15 minutes per microtiter plate in high-throughput mode using a 2-second acquisition, less than 44 minutes in standard mode using a 10-second acquisition
Carryover: <0.5% High-throughput mode; <0.75% Standard mode


Video Description:
The video below gives an overview of the BD LSRFortessa Cell Analyzer Flow Cytometer, and also demonstrates its functionality.

Documentation:
Datasheet: BD LSRFortessa Cell Analyzer Flow Cytometer

Brochure: High Throughtput Sampler

Brochure: BD LSRFortessa Cell Analyzer Flow Cytometer

Manual: BD LSRFortessa Cell Analyzer Flow Cytometer

This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*LO* Long Crate ESQ
750#

Please do not hesitate to contact us if you have any questions.
 

 

All equipment warranty request must have a support case opened within the 60-day warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.

 

Tags: BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS - AV , Flow Cytometers
Safe Shipping
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Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies