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BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors w/ BD FACSFlow Supply System and High Throughput Sampler - AV

BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors w/ BD FACSFlow Supply System and High Throughput Sampler - AV
BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors w/ BD FACSFlow Supply System and High Throughput Sampler - AV
  • Stock: In Stock
  • Brand: BD
  • Product Code: 50956
  • Condition: Pre-Owned

BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors with BD FACSFlow Supply System and High Throughput Sampler

Pre-owned instrument

• Fully Functional
• Serviced & in Stock
• Current model
• Good cosmetic condition
• Extended Collection Bed Upgrade
Continental US Warranty 120 Days & 60 Days International.
• Options for Extended warranty, Installation and Training may be available

Here is what you will receive:
•BD FACSymphony A3 Flow Cytometer (Made in 2021.  New around $300,000.00)
• Power Conditioner
• Fluidics Supply Station
• Fluidics hoses
• Pressure Vessel with holder
• Waste container
• HTS system
• Desktop PC running BD FACSDiva software ver9.1 (w/ monitor, keyboard, mouse)
• Communication and power cables

BD FACSymphony A3 Flow Cytometer System Configuration: 
Flow Cytometer:
• Optics:(3) Lasers, (14) Colors, (16) Detectors:
• Forward scatter detector: Photodiode with a 488/10 bandpass (BP) filter for the 488-nm laser
• Side scatter detector: PMT with a 488/10 BP filter for the 488-nm laser
• 488nm blue laser: 780/60, 710/50, 610/20, 575/25, 530/30
• 405nm violet laser: 780/60, 710/50, 670/30, 610/20, 470/15, 431/28
• 637nm red laser: 780/60, 710/50, 670/30

• Fluorescence sensitivity: may vary dependent on the combination of laser and filter selections
• Fluorescence resolution: coefficient of variation (CV)
• PI: Area, <3.0%, full G0 /G1 peak for PI-stained CEN
• Fluorescence linearity: doublet/singlet ratio: PI-stained CEN: 1.95–2.05 (488-nm laser)
• FSS sensitivity: sensitivity enables separation of fixed platelets from noise
• FSS resolution: scatter performance is optimized for resolving lymphocytes, monocytes and granulocytes
• SS resolution: scatter performance enables separation of 0.5-um beads from noise
• Acquisition rate: 40,000 events/second with bead

• Sample flow rates three modes: RUN, STANDBY and PRIME.
• Continuously adjustable flow rate, plus three preset flow rates: LO: 12 uL/min; MED: 35 uL/min; HI: 60 uL/min

• Instrument dimensions (W x D x H) 83.8 x 76.2 x 80 cm (33 x 30 x 31.5 in)
• Weight 167.9 kg (379 lb)
• Power Operation at 100/115/230 VAC and 50 or 60 Hz

High Throughput Sampler:
• Automates and accelerates sample acquisition
• Compatible with 96- and 384-well plates
• <0.5% sample carryover in high-throughput mode

FACSFlow Supply System:
• Increases capacity and ease of use while maintaining a stable fluidics pressure
• Reduces daily maintenance by incorporating a 20-L BD FACSFlow Cubitainer

• Extended Warranty
• Installation
• Training

QC Testing performed by our in-house service team:
• The BD FACSymphony A3 Flow Cytometer was successfully powered on by our team, ensuring its readiness for operation.
• Communication between the cytometer and its operating software was established and verified by us, confirming seamless integration.
• Our team completed the fluidics priming and sequence operations, achieving optimal system preparation without issues.
• Comprehensive system cleaning, including long clean and flow cell clean, was meticulously performed by us, ensuring the system's cleanliness and functionality.
• The high-throughput ssampler (HTS) functionality underwent rigorous testing by our specialists, with all systems confirmed as fully operational.
• We conducted and successfully confirmed the processes of homing, priming, and cleaning, of the (HTS) maintaining the system's efficiency.
• Laser alignment was precisely performed by our team, with accuracy and alignment confirmed to meet operational standards.
• Verification of all channels, using test beads, was conducted by us, ensuring each channel's functionality and readiness for accurate analysis using CS&T beads.

Instrument Overview:
• The BD FACSymphony A3 is designed for high-throughput cell analysis, enhancing lab efficiency.
• Features advanced fluidics and optical systems for precise sample handling and analysis.
• Supports multi-parametric analysis with high sensitivity and specificity.
• Equipped with a user-friendly interface, simplifying complex experimental setups.
• The instrument's modular design allows for easy upgrades and customization.
• Offers robust data management and analysis tools, streamlining workflow.

Instrument Application:
• Immunophenotyping
• Cell Cycle Analysis
• Apoptosis Detection
• Intracellular Cytokine Staining
• Cancer Research
• Functional Assays
• Multiplex Assays
• Receptor Occupancy
• Microbial Studies

• Datasheet: BD FACSymphony A3 Flow Cytometer Datasheet
• Brochure: BD FACSymphony A3 Flow Cytometer Brochure
• Manual: BD FACSymphony A3 Flow Cytometer Manual

Video Overview:

Why should you buy from us?

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and instrument operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

It will work!
• Continental US Warranty 120 Days & 60 Days International.
• An extended warranty may be available.
• You get after-sales technical support.

• Smooth process from the initial quote to order fulfillment.
• Customer references are available.

This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*LA* Large Crate ESQ

Please do not hesitate to contact us if you have any questions.



All equipment warranty request must have a support case opened within the 60-day warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.


Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies