BD FACSMelody Cell Sorter (3)Lasers/(9)Colors/(11)Detectors 

For Sale BD FACSMelody Cell Sorter (3)Lasers/(9)Colors/(11)Detectors 

Pre-owned instrument

• Current model
• Serviced & in Stock
• Good cosmetic condition
• Warranty

Here is what you will receive:

• BD Biosciences FACSMelody Cell Sorter
• 100um Nozzle (Qty:1)
• Closed-Loop Nozzle (Qty:1)
• 1.0 Neutral Density Filter (Qty:1)
• 1.5 Neutral Density Filter (Qty:1)
• 2.0 Neutral Density Filter (Qty:1)
• Aerosol Shield (Qty:1)
• Sort Block Splash Shield (Qty:1)
• 1.5mL/2.0mL Tube Holder for 2-Way Sorting (Qty:1)
• 5.0mL Tube Holder for 2-Way Sorting (Qty:1)
• 5.0mL Tube Holder for 4-Way Sorting (Qty:1)
• 5.0mL Temperature Control Tube Holder for 2-Way Sorting (Qty:1)
• 1.5mL Temperature Control Tube Holder for 2-Way Sorting (Qty:1)
• 1.5mL Temperature Control Tube Holder for 4-Way Sorting (Qty:1)
• Tube Holder Adapter (Qty:1)
• Compatible chiller will be provided (We used an internal testing chiller to verify system operation)
• JUN-AIR 87R-4P Oil-Free Air Compressor
• Air Supply Tubing
• Stainless Steel Pressure Vessel for Sheath Supply
• Sheath Tubing & Level Sensor
• Waste Tubing & Level Sensor
• Fluidics Harness/Umbilical
• Computer/Workstation with FACSChorus Software
• De Novo FCS Express analysis software (1-Year Subscription) RUO version
• Computer Monitor, Mouse, and Keyboard
• Power & Communication Cables

When you purchase a flow cytometer from Boston Industries, you also receive a 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

• User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
• Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
• Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
• High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
• Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
• With FCS Express 7, you will have the latest Research Use analysis software.

BD FACSMelody Cell Sorter Configuration:

Optics: (4-2-3) configuration

• (3) Lasers: 405nm Violet / 488nm Blue / 640nm Red
• (9) Colors simultaneously / (11) Detectors
•  Forward Scatter Channel 0 (488/10 blue laser)
•  405nm violet laser: Channel A (755LP), Channel B (528/45), Channel C (448/45)
•  488nm blue laser: Channel A (783/56), Channel B (700/54), Channel C (586/42), Channel D (527/32), Channel E (488/15)
•  640nm red laser: Channel A (783/56), Channel B (660/10)

Cell sorting collection:

• 4-way sorting: 1.5-, 2.0-, and 5.0-mL tubes
• 2-way sorting: 1.5-, 2.0- and 5.0-mL tubes
• Fluorescence sensitivity: FITC: <110 molecules (MESF-FITC); PE: <30 molecules (MESF-PE)
• Droplet sorting: 34,000 drops per second
• Acquisition rate: max 40,000 events per second, independent of the number of parameters.
• Air supply: 80–90 psi, regulated, filtered (<5 ppm), dry, oil-free
• Dimensions (W x D x H) Cell sorter: 49.5 x 55.9 x 48.3 cm (19.5 x 22 x 19 in.)
• Dimensions (W x D x H) Electronics box: 50.8 x 55.9 x 48.3 cm (20 x 22 x 19 in.)
• Weight: Cell sorter: 40.75 kg (89.8 lb)
• Weight Electronics box: 36.25 kg (79.9 lb)

Customization/Options: 

We can also add a circulating chiller if one is required.

Testing performed by our in-house service team:

The BD FACSMelody Cell Sorter passed some tests and was found to be functional:

• We powered on the BD FACSMelody Cell Sorter.
• We established a connection with the FACSChorus v2.0 Software.
• We performed the fluidics startup and stream prime.
• We monitored the sheath and waste level sensing.
• We ran several fluidics cleaning cycles.
• We utilized the sheath filter purge function.
• We completed the sample acquisition and backflush.
• We created and ran the experiment.
• We tested the automated stage alignment and operation.
• We adjusted the gain and gating.
• We acquired clean data using 8-Peak Fluorospheres.
• We ran the flowcell clean and shutdown protocols.

Instrument Overview:

• The BD FACSMelody Cell Sorter is equipped with three lasers: blue (488 nm), red (640 nm), and violet (405 nm).
• It can detect up to nine different fluorescent colors, enabling multi-parametric analysis.
• This instrument features ten detectors, ensuring precise identification and sorting of cell populations.
• The BD FACSMelody Cell Sorter provides high sensitivity and resolution for various research needs.
• Designed for ease of use, the sorter supports a wide range of fluorochromes and assays.
• The BD FACSMelody Cell Sorter's robust design ensures reliable performance in diverse laboratory settings.

Instrument Application:

• Immunophenotyping
• Stem cell research
• Cancer research
• Drug discovery
• Single-cell analysis

The key features:

• 4-2-3 configuration
• Detects nine fluorescent colors
• High sensitivity and resolution
• User-friendly design
• Reliable performance for labs

 De Novo FCS Express Software Features

• Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
• Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
• Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
• Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
• Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
• Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
• Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
• Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

 Benefits of FCS Express Software

• Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
• User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
• Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
• Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
• Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
• Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
  Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
• Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

Additional technical info:

• Datasheet: BD FACSMelody Cell Sorter Datasheet
• Analysis Software: FCS Express Analysis Software
• Brochure: BD FACSMelody Cell Sorter Brochure
• Quick Reference Guide: BD FACSMelody Cell Sorter Quick Reference Guide
• Manual: BD FACSMelody Cell Sorter Manual

Note: Subscription is for the Research Use Only (RUO) version. 

Video Overview:

About us

How we pack

Why should you buy from us?

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and instrument operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

• It will work!
• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.

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