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Agilent Fragment Analyzer 5300 Capillary Electrophoresis

Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Agilent Fragment Analyzer 5300 Capillary Electrophoresis
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

  • Stock: In Stock
  • Brand: Agilent
  • Product Code: 52731
  • Condition: Pre-Owned
$21,995.00
CLICKABLE TABS BELOW

For Sale Agilent 5300 Fragment Analyzer

 

 Pre-owned instrument

 

  • Fully Functional
  • Current Model
  • Serviced & in Stock
  • Good cosmetic condition
  • Warranty
  • An extended warranty option and training may be available.

 

Made in 2019

 

Here is what you will receive:

  • Agilent 5300 Fragment Analyzer
  • Capillary Array
  • (4) Marker/sample PCR microplate (semi or non-skirted) holders
  • (2) Deep-well microplate holders
  • (2) 250ml bottle
  • 50ml bottle
  • 500ml waste bottle
  • Computer with Fragment Analyzer ProSize data analysis software
  • Fragment Analyzer controller software
  • Syringe for valve flush
  • Communication and power cables

 

Please note: The Capillary Array is a consumable and has a one-year or 1000-injection lifespan. A new one will need to be purchased from Agilent for around $3540. 

 

Agilent 5300 Fragment Analyzer Configuration:

  • Instrument Applications: NGS libraries, cell-free DNA, genomic DNA, large fragment DNA, PCR fragments, total RNA and IVT RNA
  • Analysis Method: fluorescence-based capillary electrophoresis using intercalating dye
  • Capillary Array: compatible with 48 and 96 capillary arrays
  • Minimum Sample Volume: 25 uL of liquid for injection; as little as 2.5 uL of actual sample required
  • Sample Capacity: 3 sample trays, each holding 1x 96 well PCR microplate
  • Compatible Microplates: 96 well semi or non-skirted PCR plates for markers and samples, 96 deep-well plates for buffer and waste
  • Number of Samples per Run: 48 or 96 (Depending on the installed Array)
  • Variable Throughput: up to 240 samples, medium throughput system
  • High Sensitivity: sensitivities down to 5 pg/uL for a single fragment, cycle time or PCR reagent
  • Resolution and Sizing Accuracy: resolve small differences between fragments, as low as 3 bp for fragments under 300 bp size

 

Customization/Options: n/a

 

Testing performed by our in-house service team:

  • We checked the power supply to ensure it provides stable output.
  • We test communication with the software to verify seamless interaction and data exchange.
  • We test the initialization and homing processes to guarantee accurate and reliable setup.
  • We check the pump operation to ensure consistent and reliable fluid movement.
  • We test the plate movement to validate precise and smooth sample handling.
  • We thoroughly test the prime function of all fluids to ensure the system is clean and functions as designed.
  • We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
  • We test the high voltage system from minimum to maximum levels to verify consistent and safe operation.
  • We performed an Optical Alignment on a Capillary Array, verifying the camera and illumination were operating fully.


Instrument Overview: 

  • Agilent 5300 Fragment Analyzer utilizes capillary electrophoresis for high-resolution nucleic acid analysis.
  • Advanced algorithms enable precise sizing and quantification of DNA and RNA fragments.
  • Quality control capabilities include assessing genomics workflows with exceptional accuracy.
  • The instrument streamlines the evaluation of PCR and NGS library preparations.
  • Robust technology ensures not only accurate quantification but also comprehensive integrity assessment for diverse samples in molecular biology research.

 

Instrument Application: 

Agilent 5300 Fragment Analyzer is commonly used in a variety of assay and application examples, including:

  • Nucleic acid fragment analysis
  • Quality control in genomics workflows
  • Size determination of DNA and RNA fragments
  • Assessment of PCR and NGS library preparations
  • Quantification and qualification of sample integrity
  • High-throughput DNA and RNA analysis for research and diagnostics

 

The key features of Agilent 5300 Fragment Analyzer:

  • High-precision DNA/RNA analysis
  • High-resolution separation
  • Medium- to high-throughput separation
  • User-friendly interface
  • Reduced sample analysis time
  • Broad assay portfolio
  • Controlled sample handling
  • Seamless switching between applications

 

Additional technical info:

 

Video Overview: 

 

Why should you buy from us?

 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping.
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
  • Many upgrade and repair parts are in stock.
  • Remote installation and instrument operation training are available.
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 

  • It will work!
  • Warranty
  • An extended warranty may be available.
  • You get after-sales technical support.
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

*ME* Medium Crate
350#

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What steps do you take to verify, service, and confirm the functionality of the Agilent 5300 Fragment Analyzer?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

 

  • We checked the power supply to ensure it provides stable output.
  • We test communication with the software to verify seamless interaction and data exchange.
  • We test the initialization and homing processes to guarantee accurate and reliable setup.
  • We check the pump operation to ensure consistent and reliable fluid movement.
  • We test the plate movement to validate precise and smooth sample handling.
  • We thoroughly test the prime function of all fluids to ensure the system is clean and functions as designed.
  • We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
  • We test the high voltage system from minimum to maximum levels to verify consistent and safe operation.
  • We performed an Optical Alignment on a Capillary Array, verifying the camera and illumination were operating fully.

 

What supplies do I need?

You will need the consumables starting out: 

 

 

Can I view the Agilent 5300 Fragment Analyzer in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or feel free to request a virtual Zoom demo of the instrument you are interested in.

 

 What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warrantied for both parts and labor.
  • The customer should retain all packing materials during the warranty period. 

 

Installation & Training:

 

Option 1 – In Person

If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.

 

Option 2 – Remote/Virtual

Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.

 

Both training options include:

  • Review of the instrument and its capabilities.
  • General instrument and software operation.
  • Review of the recommended maintenance procedures.

 

 We generally recommend these training services if you never worked with this instrument.

 

What should I watch out for when buying Agilent 5300 Fragment Analyzer used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components. 
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

 

  • We check the power supply to ensure it met the required specifications.
  • We test communication with the software to verify seamless interaction and data exchange.
  • We test the initialization and homing processes to guarantee accurate and reliable setup.
  • We check the pump operation to ensure consistent and reliable fluid movement.
  • We test the plate movement to validate precise and smooth sample handling.
  • We thoroughly test the fluid prime function of all fluids to ensure proper functionality and prevent any potential issues.
  • We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
  • We test the high voltage system from minimum to maximum levels to verify consistent and safe operation.

 

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.

 

 Do you have an Agilent 5300 Fragment Analyzer on hand?
  • This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.

 

Here are some of the reasons for our price.

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We have to purchase any missing required accessories to make the instruments complete.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
  • We guarantee that the instrument will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

 How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

 

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

 How do you pack? Have you packed and shipped Fragment Analyzer Instruments in the past? Do you ship Internationally?

Packing

  • Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments. 

 

*ME* Medium Crate
350#

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why.  Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement. 
  • Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.

 

 Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.   

 

 I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

 

 

Agilent Fragment Analyzer Quick Guide

 

(Click on the PDF below to scroll thru it)

 

(Click on the empty blank/white space to scroll thru)

 

 

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies