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Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector

Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

  • Stock: In Stock
  • Brand: Waters
  • Product Code: 49475
  • Condition: Pre-Owned
$22,995.00
CLICKABLE TABS BELOW

For Sale Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector


Pre-owned instrument

 

  • Fully Functional
  • Current model
  • Serviced & in Stock
  • Good cosmetic condition
  • Warranty
  • An extended warranty option and training may be available.

 

Here is what you will receive:

  • Acquity H bioQuaternary Solvent Manager (bio-QSM #186015041)
  • Acquity H bioSample manager-FTN with 50uL extension loop (bio SM-FTN #186015040)
  • Acquity TUV detector (TUV #186015031) w/ Analytical 500nL Flow Cell
  • Acquity Column heater/cooler 30cm (30-cm CHC #186015011)
  • Acquity Column heater (CH-A #186015042)
  • Solvents Tray
  • Inter-communication cables
  • Standard power cords.

 

Please Note: Computer and Empower software licenses will need to be purchased separately. They are not included. The computer was used for testing only.

 

Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector Configuration:

  • Dwell volume (total system): <400 uL (includes standard 100 uL mixer)
  • Integrated leak management: leak sensors, as standard, and safe leak handling
  • Quantum synchronization: injection synchronization between the pump and sample manager enhances retention time reproducibility
  • Settable flow rate range: 0.001 to 2.200 mL/min in 0.001 mL increments
  • Maximum operating pressure: 15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min
  • pH range: 2 to 12
  • Unattended operation: leak sensors and safe leak handling, full 96-hour diagnostic data display through console software
  • Cycle time: <30 s inject-to-inject

 

BioQuaternary Solvent Manager:

  • Number of solvents: up to four solvents in any combination
  • Solvent degassing: Integrated vacuum degassing, four chambers; one additional chamber for the SM-FTN purge solvent
  • Solvent blending: automated, on-line pH, ionic strength, and organic modifier blending from pure solvents with Auto-Blend Plus Technology
  • Gradient formation: Low-pressure mixing, quaternary gradient
  • Gradient profiles: 11 gradient curves [including linear, step (2), concave (4), and convex (4)]
  • Primary check valve: Intelligent Intake Valve (i2 Valve)
  • Flow accuracy ±1.0% at 0.5 to 2.0 mL/min using 100% A (with i2 Valve); Backpressure 600 to 1000 psi with degassed H20
  • Flow precision 0.075% RSD or ±0.020 min SD, whichever is greater, based on six replicates (with i2 Valve); H2O/MeOH (60:40) via Auto-Blend Plus Technology
  • Compressibility compensation: automatic and continuous
  • Priming: wet priming can run at flow rates up to 4 mL/min
  • Pump seal wash: equipped with an automated wash system to flush the rear of the high pressure seal and the plunger
  • Flow ramping Range: 0.01 to 30.00 min to reach 2.00 mL/min; Default: 0.45 min to reach 2.00 mL/min

 

BioSample manager-FTN:

  • Injection volume range: 0.1 to 50 uL, up to 1,000.0 uL with optional extension loops
  • Number of sample plates: any two of the following: 96 and 384 microtiter plates, 48 position 2.00-mL vial plates, 48 position 0.65-mL micro-centrifuge tube plates, 24 position 1.50-mL micro-centrifuge tube plates
  • Maximum sample capacity: 768 in two 384-well plates or 96 in 2-mL vial holders, four additional positions for dilution functions
  • Sample compartment temperature range: 4DegC to 40DegC, settable in 0.1 DegC increments
  • Temperature accuracy: ±0.5DegC at sensor
  • Temperature stability: ±1.DegC at sensor
  • Injection needle wash: Integral, active, programmable
  • Minimum sample required: 3 uL residual, using total recovery 2-mL vials (zero offsets)
  • Advanced Sample Manager capabilities: auto-dilution, auto-addition, and load-ahead

 

UPLC TUV Detector:

  • Wavelength range: 190 to 700 nm
  • Wavelength accuracy: ±1 nm
  • Sampling rate: up to 80 points/s
  • Light source: deuterium lamp
  • Flow Cell: Analytical 500nL Flow Cell
  • Dispersion volume: ≤0.8 uL (analytical flow cell);
  • Flow cell design: light-guiding UPLC intelligent technology flow cell
  • Path length: 10 mm (analytical flow cell)
  • Flow cell volume: 500 nL (analytical flow cell)
  • Pressure limit: 1000 psi (all flow cells)
  • Optical bandwidth: 5 nm

 

Column heater/cooler 30cm:

  • Column capacity: accommodates multiple columns, 20 to 300 mm in length, between 2.1 - 7.8 mm internal diameter (I.D.)
  • Compartment temperature control: 4-65DegC  in 0.1DegC increments
  • Column compartment temperature accuracy: ±0.8 DegC at the sensor
  • Compartment operational temperature range: cooling: 15 DegC below ambient; heating: 5DegC above ambient to 65DegC

 

Column heater:

  • Column capacity: Single column, up to 150 mm in length, up to 4.6 mm internal diameter (I.D.)
  • Column compartment temperature range: 5-90DegC, settable in 0.1DegC increments
  • Column compartment temperature accuracy: ±0.5 DegC at the sensor
  • Column compartment temperature stability ±0.3 DegC at the sensor
  • Solvent conditioning: active pre-heating

 

Customization/Options:

Other detectors, flow cell options, and sample loops might be available. Please inquire with our sales team.

 

Testing performed by our in-house service team:

  • We inspect the Waters Acquity UPLC-H Bio Liquid Chromatography System for physical damage.
  • We check for seamless communication with the Empower software.
  • We run samples and tests that allow us to perform IQ/OQ/PQ.

BioQuaternary Solvent Manager:

  • We check to ensure the proper initialization of the Solvent Manager.
  • We thoroughly assess the stability and consistency of solvent delivery.
  • We verify the accuracy of flow rates.
  • We evaluate the system's performance in running gradient tests.
  • We test the functionality and performance of the pressure limiter.
  • We verified that the seal-washing pump is operational efficiently.
  • We perform degasser health checks to verify the degasser is functioning correctly.

bioSample manager-FTN:

  • We check to ensure the proper initialization of the sample manager.
  • We evaluate the accuracy of the sample cooler set temperature for consistency and precision.
  • We verify the reproducibility of sample handling processes.
  • We check the column oven's temperature accuracy within specified standards to ensure precise control for optimal chromatographic separation.
  • We check the syringe's integrity and perform a Sample Syringe Leak Test.
  • We inspect the integrity of the needle seal by performing the Needle Seal Readiness Test.
  • We perform a quick test of the leak detector.
  • We perform the system's reproducibility tests, ensuring consistent and reliable performance across multiple runs and providing confidence in the accuracy of chromatographic results.

TUV detector:

  • We perform checks to evaluate the proper initialization of the TUV detector.
  • We check the remaining lamp life to ensure optimal performance.
  • We run QC sample tests to verify accurate and reliable detection capabilities.

Column heaters:

  • We run the initialization of the column heater.
  • We run tests to verify the accuracy and stability of the column oven temperature.
  • We perform ramping stress tests to verify performance.

 

Instrument Overview: 

  • Waters Acquity UPLC H-Class Bio Liquid Chromatography System provides high-performance separation and analysis capabilities.
  • Equipped with a TUV Detector to enable sensitive detection in various applications.
  • Offers advanced features for precise quantification and identification of compounds.
  • Streamlined design for efficient sample processing and data acquisition.
  • Reliable performance is ideal for bioanalytical and pharmaceutical research labs.
  • Ensures optimal chromatographic results with enhanced sensitivity and speed.

 

Instrument Application:

  • Peptide mapping
  • Ion exchange
  • Size exclusion
  • Hydrophilic interaction
  • Reversed-phase
  • Glycan analysis
  • Oligonucleotide analysis

 

The key features of Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector: 

  • Advanced separation
  • Sensitive TUV detection
  • Auto-Blend Plus technology
  • Multi-solvent blending
  • Accurate column temperature control
  • Modular design
  • Reliable bioanalysis

 

Additional technical info:

TUV Detector Flow cell options:

  • Analytical: 500 nL volume, 10 mm pathlength
  • High sensitivity: 2400 nL volume, 25 mm pathlength
  • Stainless steel: 1.5 uL volume, 5 mm pathlength
  • Titanium: 1.5 uL volume, 5 mm pathlength
  • Low dispersion analytical flow cell: 500 nL, 10 mm pathlength
  • Low dispersion microbore flow cell: 250 nL, 10 mm pathlength
  • Microscale: 100 nL, 10 mm pathlength

 

 

Video Overview:

About us

How we pack

 

Why should you buy from us?

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping.
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Many upgrade and repair parts are in stock.
  • Remote installation and instrument operation training are available.
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

  • It will work!
  • Warranty
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*SM* Small Crate
300#

 

COMMON QUESTIONS AND ANSWERS BELOW

What steps do you take to verify, service, and confirm the functionality of the Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

 

  • We inspect the Waters Acquity UPLC-H Bio Liquid Chromatography System for physical damage.
  • We check for seamless communication with the Empower software.
  • We run samples and tests that allow us to perform IQ/OQ/PQ.

 

BioQuaternary Solvent Manager:

  • We check to ensure the proper initialization of the Solvent Manager.
  • We thoroughly assess the stability and consistency of solvent delivery.
  • We verify the accuracy of flow rates.
  • We evaluate the system's performance in running gradient tests.
  • We test the functionality and performance of the pressure limiter.
  • We verified that the seal-washing pump is operational efficiently.
  • We perform degasser health checks to verify the degasser is functioning correctly.

 

bioSample manager FTN:

  • We check to ensure the proper initialization of the sample manager.
  • We evaluate the accuracy of the sample cooler set temperature for consistency and precision.
  • We verify the reproducibility of sample handling processes.
  • We check the column oven's temperature accuracy within specified standards to ensure precise control for optimal chromatographic separation.
  • We check the syringe's integrity and perform a Sample Syringe Leak Test.
  • We inspect the integrity of the needle seal by performing the Needle Seal Readiness Test.
  • We perform a quick test of the leak detector.
  • We perform the system's reproducibility tests, ensuring consistent and reliable performance across multiple runs and providing confidence in the accuracy of chromatographic results.

 

TUV detector:

  • We perform checks to evaluate the proper initialization of the TUV detector.
  • We check the remaining lamp life to ensure optimal performance.
  • We run QC sample tests to verify accurate and reliable detection capabilities.

 

Column heaters:

  • We run the initialization of the column heater.
  • We run tests to verify the accuracy and stability of the column oven temperature.
  • We perform ramping stress tests to verify performance.

 

What supplies do I need?

You will need the consumables starting out:

Can I view the Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warrantied for both parts and labor.
  • The customer should retain all packing materials during the warranty period.

 

Installation & Training:

 

Option 1 – In Person

If your lab is located within two hours of Boston, we may be able to offer in-person installation and training in person.

 

Option 2 – Remote/Virtual

Our remote training is conducted via Zoom, allowing us to significantly reduce its cost and offer it to customers outside our travel area.

 

Both training options include:

  • Review of the instrument and its capabilities.
  • General instrument and software operation.
  • Review of the recommended maintenance procedures.

 

We generally recommend these training services if you never worked with this instrument.

What should I watch out for when buying a Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

The System as a whole:

 

  • We inspect the Waters Acquity UPLC-H Bio Liquid Chromatography System for physical damage.
  • We check for seamless communication with the Empower software.
  • We run samples and tests that allow us to perform IQ/OQ/PQ.

BioQuaternary Solvent Manager:

 

  • We check to ensure the proper initialization of the Solvent Manager.
  • We thoroughly assess the stability and consistency of solvent delivery.
  • We verify the accuracy of flow rates.
  • We evaluate the system's performance in running gradient tests.
  • We test the functionality and performance of the pressure limiter.
  • We verified that the seal-washing pump is operational efficiently.
  • We perform degasser health checks to verify the degasser is functioning correctly.

bioSample manager FTN:

 

  • We check to ensure the proper initialization of the sample manager.
  • We evaluate the accuracy of the sample cooler set temperature for consistency and precision.
  • We verify the reproducibility of sample handling processes.
  • We check the column oven's temperature accuracy within specified standards to ensure precise control for optimal chromatographic separation.
  • We check the syringe's integrity and perform a Sample Syringe Leak Test.
  • We inspect the integrity of the needle seal by performing the Needle Seal Readiness Test.
  • We perform a quick test of the leak detector.
  • We perform the system's reproducibility tests, ensuring consistent and reliable performance across multiple runs and providing confidence in the accuracy of chromatographic results.

TUV detector:

 

  • We perform checks to evaluate the proper initialization of the TUV detector.
  • We check the remaining lamp life to ensure optimal performance.
  • We run QC sample tests to verify accurate and reliable detection capabilities.

Column heaters:

 

  • We run the initialization of the column heater.
  • We run tests to verify the accuracy and stability of the column oven temperature.
  • We perform ramping stress tests to verify performance.

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.

Do you have a Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector on hand?
  • This is a common Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector instrument elsewhere, possibly for less.

Here are some of the reasons for our price.

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We must purchase any required or missing accessories to complete the instruments.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing and shipping sensitive instruments.
  • We guarantee that the instrument will be functional.

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

How do you pack? Have you previously packed and shipped Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector? Do you ship Internationally?

Packing

  • Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

 

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

 

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

 

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

  • You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies