- Stock: In Stock
- Brand: Sartorius
- Product Code: 51199
- Condition: Pre-Owned
Here is a Sartorius iQue 3 BR Flow Cytometer with IntelliCyt Fluidics Station (2)Lasers/(6)Colors/(8)Detectors
The cytometer is 2020 or newer
New can be around to $150,000
Predecessor to:
Current model
Condition:
This Sartorius iQue 3 BR Flow Cytometer is in good working condition and has a low total runtime of 102 hours.
Please see exact photos and video below.
Testing we performed:
The Sartorius iQue 3 BR Flow Cytometer passed all tests and was found to be fully functional:
Tested Power and Initialization: PASSED
Tested Communication with ForeCyte Software: PASSED
Tested Fluidics Prime and Purge Protocols: PASSED
Tested Fluidics Cleaning Protocols: PASSED
Tested Peristaltic Pump Operation: PASSED
Tested Sheath Pump Operation: PASSED
Tested Bottle Station Liquid Level Detection: PASSED
Tested Plate Gripping and Shaking: PASSED
Tested Sample Tube Vortexing Function: PASSED
Tested Enhanced Rinse Station Operation: PASSED
Tested Probe Contact Sensing Function: PASSED
Tested Deck Height Calibration Function: PASSED
Tested QC with iQue3 Screener PLUS Validation Beads: PASSED
System consists of:
Sartorius iQue 3 BR Flow Cytometer
Sartorius IntelliCyt Fluidics Station
Fluidics Supply and Waste Bottles
Brand New iQue Enhanced Cartridge Kit
Desktop Computer with iQue Forecyt Software ver9.0 (w/ monitor, keyboard, mouse)
Communication and Power Cables
Configuration and Setup:
Sartorius iQue 3 Blue-Red (BR)
(2) Lasers: 20mW 488nm Blue, 40mW 640nm Red
(6) Simultaneous Color Channels using PMTs
- Forward Scatter Channel
- Side Scatter Channel
(8) Total Detection Channels
Channel Filters: (Blue) 530/30, 572/28, 675/30, 780/60 (Red) 675/30, 780/60.
Fluorescence sensitivity: FITC < 75 MESF; PE < 50 MESF; APC < 20 MESF
Minimum particle size detection: 0.5 µm
Detection Rate: up to 35,000 events/second
Dynamic range of detection: > 7 decades
Plate Types: 96-well and 384-well microplates
Minimum Sampling Time: < 5 minutes/96 wells, < 20 minutes/384 wells
Minimum assay volume requirements 10 µL
Minimum sample aspiration 1 µL
Carryover < 2% for typical no-wash assays
Automated plate shaker: up to 3,000 rpm
Features: Foil-sealed plate processing; Volumetric cell counting (< 10% CV)
Video Description:
The video below gives an overview of the Sartorius iQue 3 BR Flow Cytometer, and also demonstrates its functionality.
Documentation:
Datasheet: Sartorius iQue 3 BR Flow Cytometer
Brochure: Sartorius iQue 3 BR Flow Cytometer
Manual: Sartorius iQue 3 BR Flow Cytometer
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*LA* Large Crate ESQ
500#
Please do not hesitate to contact us if you have any questions.
Excitation Laser | Emission Filter (nm) | Channel | Recommended Dyes |
Blue - 488nm | 530/30 | BL1 | FITC |
572/28 | BL2 | PE, PI | |
675/30 | BL4 | PerCP-C5, Cy5.5 | |
780/60 | BL5 | PE-Cy 7, Qdot 800 | |
Red- 640nm | 675/30 | RL1 | APC |
780/60 | RL2 | PC-Alexa Fluor 750 | |
FSC | Yes | ||
SSC | Yes |
All equipment warranty request must have a support case opened within the 60-day warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.
Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.
Here we are showing you our standard packing process
1. All items are different, but they all follow the same principle when packaging.
2. Start with a well-constructed crate
A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.
3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.
4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.
5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.
6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.
7. Final step is to photograph everything inside the crate and then seal it with screws.