Nikon Eclipse TE2000-E Inverted Fluorescence Motorized PFS Perfect Focus Microscope with PH Pred TI2-E

For Sale Nikon Eclipse TE2000-E Inverted Fluorescence Phase Contrast Motorized Microscope with Perfect Focus PFS Pred Ti2-E

Pre-owned microscope (this microscope can be fully customized)

• Fully Functional
• Serviced & in Stock
• Good cosmetic condition
• Comes with Brand New Fluorescence Filter Sets
• Continental US Warranty 120 Days &  60 Days International.
• An extended warranty option and training package are available.

The Predecessor to Nikon Eclipse TI2-E Inverted Fluorescence Microscope with Perfect Focus

This is what you will receive before customizing this microscope:

Standard Nikon Eclipse TE2000-E PFS Microscope Configuration:

Microscopy Techniques:

Brightfield, Fluorescence, and Phase Contrast

Microscope Base:

• Nikon Eclipse TE2000-E Inverted Base with 4 camera ports
• Illumination arm
• TE-HUBC Controller

Microscope Head:

• Binocular Head
• Two Eyepieces

Fluorescence Light Path:

• Nikon fluorescence lightpath

Fluorescence Illumination:

• EXFO X-Cite 120w Metal Halide supply
• Light Guide
• Collimating Adapter

Fluorescence filter cube turret:

• Motorized 6-position filter cube turret

Fluorescence filters:

• Three empty filter cubes
• Three Brand New filter sets of either DAPI, FITC, Texas Red, CY5, CY3

Brightfield Light Path:

• Illumination Tower

Brightfield Illumination:

• Nikon 100 Watt halogen light source
• Nikon 100 Watt halogen power supply

Objective Turret:

• Motorized 6-position objectives turret
• PFS (Perfect Focus Drift System)

Objectives:

• 4x/0.13  CFI Phl Plan Fluor objective
• 10x/0.30 CFI Ph1 Plan Fluor objective
• 20x/0.75 CFI Plan Apo VC objective

Condenser:

• Nikon Mechanical Phase Contrast condenser

Stage:

• Motorized XY Stage
• Stage Controller
• Joystick

Camera ports:

• Left Port
  
• Right Port
• Front Port
• Bottom Port

Camera adapter:

• 1x C-mount adapter 

Camera:

• CMOS USB 3.0 10MP Camera

Computer & Software:

• Dell laptop computer with Windows 10
• Micromanager w/Image J analysis software
• Camera software

Accessories:

• Necessary power and communication cables

This configuration includes any 3 of these new filter sets.

• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

Automated/motorized capabilities of this microscope:

Installed automation/motorized components:

• Motorized lightpath
  
• Motorized/automated Z-Axis Drive
• Motorized filter cube turret
• Motorized objective(nosepiece) turret
• Motorized drift focus (PFS) control 
• Motorized XY Stage

Benefits of motorized/Automated Z-Axis drive:

• Automatic parfocality adjustment
• The ability to recall previously saved focus levels
• Automated management of Z positioning/ Fine-coarse control

 How the Nikon Perfect Focus System Works

• Uses IR Light Source: Employs infrared light to illuminate samples, reducing photodamage and bleaching in live cells which enhances their viability during extended observations.
  
• Dedicated Optical Path: Incorporates a dichroic mirror in the optical pathway that directs IR light to the sample while allowing visible light to pass through, optimizing multi-modal imaging techniques.
• Reflected Light Detection: Detects IR light reflections from the interface between the cover glass and the sample, critical for maintaining focus.
• Focus Offset Detection: Continuously monitors the position of reflected IR light to identify any changes in focus or positioning due to environmental factors or equipment movement.
• Automatic Correction: Automatically adjusts the Z-Axis in real-time to correct any detected focus shifts or drifts, ensuring consistent, high-quality imaging.

 Benefits of Nikon Perfect Focus System(PFS):

• Easy Sample Swapping at Various Focal Ranges: Enables quick refocusing by swapping samples within the predetermined focal planes of the PFS range.
  
• Extended Observations: Facilitates the continuous monitoring of live cells for prolonged periods.
• Reduced Phototoxicity: Precision in maintaining focus diminishes the need for frequent manual adjustments, thus decreasing light exposure.
• Automated Scanning of Multi-well Plates: Provides automated, consistent focusing across different wells in a multi-well plate.
• Embryo Imaging Capabilities: Offers the necessary stability for imaging developing embryos over time.
• Neuron Activity Research: Allows the system to persistently focus on specific brain regions or neural cultures for extended durations.
• Decreased Photobleaching: Helps minimize light exposure by maintaining focus with fewer adjustments.
• Enhanced Z-stacking: Guarantees consistent focusing across multiple z-planes.
• Stable Time-lapse Imaging: Essential for capturing consistent focus in time-lapse sequences to accurately document temporal changes.
• Surface Topography Analysis: Critical for precisely mapping surface topographies to understand material characteristics better.
• Monitoring Drug Responses: Enables detailed tracking of cellular reactions to drug treatments over time.

This microscope's imaging capabilities:

Imaging software highlights:

• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others 
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time) 
• Multi-slice (Z coordinate/Z Stacking)  
• Multi-position (XY coordinate/Stitching/Mosaic) – only available when equipped with a motorized XY Stage

We can customize this microscope to fit your requirements:

Add Microscopy Techniques (In addition to installed Brightfield, Fluorescence and Phase Contrast)

• DIC, or Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality:

• Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives 
• We can add high-end objectives (APO)

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

• Additional filter cubes
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:

• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

Add other Motorized/Automation Capabilities:

In addition to installed motorized capabilities, the microscope can be configured

• Motorized condenser, Motorized high-speed shutter

We can configure it to work with the sample medium you need:

• Slide, Petri dish, microplate, etc.
• It may also be set up with a motorized XY stage for multi-slide or full microplate scans.

Commonly Requested Customer Upgrades

• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized XY Stage (stage, controller, joystick)

We are excited to help build and configure the right microscope for you!

Nikon Eclipse TE2000-E Perfect Focus PFS Microscope Overview

(Final microscope capabilities will depend on the modifications you request)

The Nikon TE2000-E is a sophisticated inverted microscope system designed for high-end imaging applications, especially in life sciences. This model incorporates Nikon's innovative Perfect Focus System (PFS), which plays a critical role in maintaining constant focus automatically, a particularly valuable feature for long-term live cell imaging.

Here are some key aspects of the Nikon TE2000-E with the Perfect Focus System:

• Perfect Focus System (PFS): The PFS is an optical feedback system that prevents focus drift during long-term imaging, which can be caused by environmental factors like temperature changes or mechanical vibrations. This ensures high-quality, sharp images over extended observation periods.
• Modular Design: The TE2000-E features a modular design that allows for a wide range of configurations to suit different imaging needs, including bright field, dark field, fluorescence, and DIC imaging techniques. This flexibility makes it suitable for various applications from basic research to advanced analyses.
• Advanced Imaging Capabilities: Equipped with high-quality optics and capable of accommodating a variety of light sources and detectors, the TE2000-E supports demanding imaging techniques such as FRET, FRAP, and live-cell imaging. Its design facilitates easy access to the sample, making it convenient for researchers to manipulate specimens.
• Ergonomic and User-friendly: The microscope is designed with user comfort in mind, featuring ergonomic controls and a user-friendly interface that simplifies complex imaging tasks. This makes it accessible to both novice and experienced users.
• Integration with Other Equipment: The TE2000-E can be integrated with various peripherals and software, enhancing its functionality for automated experiments and complex data analysis. This integration capability is crucial for high-throughput experiments and detailed, quantitative studies.
• Overall, the Nikon TE2000-E with the Perfect Focus System is a high-performance tool ideal for detailed and prolonged imaging sessions in research environments, providing reliability and precision in capturing dynamic biological processes.

Suited for the following applications as configured:

• Fluorescence microscopy: Ideal for fluorescent labeling and imaging of cells and tissues.
• Live Cell Imaging: Continuous monitoring of live cells over extended periods without focus drift.
  
• Time-Lapse Microscopy: Captures detailed time-lapse sequences of biological processes, ensuring consistent focus throughout.
• Fluorescence Imaging: Minimizes photobleaching and phototoxicity while capturing fluorescently labeled specimens.
• Drug Development: Observes cellular responses to pharmacological treatments over time, useful in drug discovery and testing.
• Neuroscience Research: Studies neuron activity and brain sections over long periods, maintaining precise focus for detailed observations.
• Embryology: Monitors embryonic development stages, providing stable imaging necessary for developmental biology studies.
• Cancer Research: Tracks cancer cell behaviors and interactions, crucial for understanding tumor biology and treatment effects.
• Stem Cell Research: Follows stem cell differentiation and proliferation without disrupting the focal plane.
• Immunofluorescence: Supports detailed studies of immune cell interactions and protein localization in tissues.
• Zebrafish Imaging: Ideal for imaging zebrafish embryos and larvae to study vertebrate development and genetics.
• Plant Biology: Allows for the study of plant cell dynamics and growth under various conditions.
• Material Science: Suitable for analyzing non-biological specimens, such as materials and engineered tissues, where focus stability is critical.

Other potential applications:

• Phase contrast: Enhances visibility of transparent specimens without staining.
• High-resolution imaging: Provides clear, detailed images for in-depth analysis of samples.
• Live cell observation: Enables detailed study of live cell dynamics in real-time.

• Brochure: Nikon Eclipse TE2000-E Microscope brochure
• Manual: Nikon Eclipse TE2000-E Microscope manual

Video Overview:

Check out our microscopy capabilities:

https://www.bostonind.com/our-microscopy-capabilities

How we pack

Why should you buy from us?

• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

• It will work!
• Continental US Warranty 120 Days & 60 Days International.
• An extended warranty may be available.
• You get after-sales technical support.
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.

COMMON QUESTIONS AND ANSWERS BELOW