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- Stock: SOLD
- Brand: Life Technologies
- Product Code: 50248
- Condition: Pre-Owned
Here is a Thermo Attune NxT Acoustic Focusing Cytometer AFC2 (3)Lasers/(11) Colors/(13)Detectors with Autosampler
Made by Life Technologies, now brand of Thermo Scientific
Model AFC2
Get it under service agreement with Thermo and you will likely end up paying less than 40% vs buying it new.
Buying Service agreement will give you a one year warranty and manufacturers support.
Predecessor to:
Current model
Condition:
This Thermo Attune NxT Acoustic Focusing Cytometer is in good working condition.
Eligible for Support/Remote Instation and Training Package (Is not replacement for Service contract from Thermo)
Please see the exact video and photos below.
Testing we performed:
The Thermo Attune NxT Acoustic Focusing Cytometer passed all tests and was found to be fully functional:
Startup and initialization: PASSED
Connected to software: PASSED
Tested operation of pumps: PASSED
Tested operation of vavles: PASSED
Tested communication with sampler: PASSED
Ran fluidics priming and startup sequences: PASSED
Ran several cleaning cycles (long clean, flow cell clean, etc.): PASSED
Tested functionality of Auto Sampler: PASSED
Performed baseline test with Attune performance beads: PASSED
Performed performance check with Attune performance beads: PASSED
System consists of:
Thermo Attune NxT Acoustic Focusing Cytometer AFC2
Attune NxT Gen Autosampler
Desktop PC running Attune NxT Software v5.3 (w/ license dongle, monitor, mouse and keyboard)
Communication and power cables.
Attention: if you would like to add Attune NxT Extended Fluid System EFS1 (w/ hoses, cable & umbilicals for Fluid Tray) for extended operation, please inquire with us for pricing.
Configuration and Setup:
Optics:(3) Lasers, (11) Colors, (13) Detectors
- Forward Scatter Channel: 488/10 blue laser
- Side Scatter Channel: 488/10 blue laser
- 405nm violet laser: 440/50, 512/25, 603/48, 710/50
- 488nm blue laser: 530/30, 574/26, 695/40, 780/60
- 637nm red laser: 670/14, 720/30, 780/60
Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um
Data Acquisition Speed: up to 35,000 events/second with 34 parameters, based on a 10% coincidence rate per poisson statistics
Maximum Electronic Speed: 65,000 events/second with all parameters
No-wash, no-lyse sample preparation workflow
Cycle time: 20-40 min for tube
Attune NxT Autosampler is compatible with many different plate formats, including 96-well, 384-well, and deep-well plates
<42 min for 96-well plate
<180 min for 384-well plate
Video Description:
The video below gives an overview of the Thermo Attune NxT Acoustic Focusing Cytometer, and also demonstrates its functionality.
Documentation:
Datasheet: Thermo Attune NxT Acoustic Focusing Cytometer
Brochure: Thermo Attune NxT Acoustic Focusing Cytometer
Brochure: Thermo Attune NxT Acoustic Focusing Cytometer
Manual: Thermo Attune NxT Acoustic Focusing Cytometer
Software Manual: Thermo Attune NxT Acoustic Focusing Cytometer
Autosampler Manual: Thermo Attune NxT Acoustic Focusing Cytometer
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*SM* Small Crate ESQ
200#
Please do not hesitate to contact us if you have any questions.
Excitation Laser | Emission Filter (nm) | Channel | Recommended Dyes |
Violet - 405nm | 440/50 | VL1 | Alexa Fluor 405, Pacific Blue |
512/25 | VL2 | Pacific Green | |
603/48 | VL3 | Pacific Orange, Qdot 605 | |
710/50 | VL4 | Qdot 705 | |
Blue -488 nm | 530/30 | BL1 | Alexa Fluor 488, FITC |
574/26 | BL2 | PE, PI, Alexa-Fluor 568 | |
695/40 | BL3 | PE-Alexa Fluor 700 Tri-Color, Pe-Cy 5.5 PerCP, PerCP-Cy 5.5 | |
780/60 | BL4 | PE-Cy 7, Qdot 800 | |
Red- 637nm | 670/14 | RL1 | APC, Alexa Fluor 647, Qdot 655 |
720/30 | RL2 | Alexa Fluor 680, Alexa Fluor 700 Qdot 705 | |
780/60 | RL3 | APC-Alexa Fluor 750 APC-Cy 7, Qdot 800 | |
FSC | Yes | ||
SSC | Yes |
We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend thousands of dollars and receive a damaged instrument.
Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing.
We generally ship parcels either via UPS or freight for all larger goods.
Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant).
On freight shipments, we use 2” 1.7 lb. density foam, which provides excellent cushioning and support for the instrument.
Instruments are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provides cushioning in all directions and holds instruments firmly in place.
Final step is to photograph everything inside the crate and then seal it with screws.