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Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro

Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block Pred Pro
Most Real-Time PCR Machines ship within 5-7 days
Multiple LightCyclers Sold in the last 12 months
Happy to provide Customer references available upon request
384/96 Well-block options available

 

  • Stock: In Stock
  • Brand: Roche
  • Product Code: SM100253-1
  • Condition:
$7,995.00
CLICKABLE TABS BELOW

For Sale Roche LightCycler 480-II Advanced PCR System with 384 Well Thermocycler Block

 

Predecessor to:

Roche LightCycler Pro

 

Condition, availability, and customization

  • Fully Functional
  • Fully Serviced 
  • Good condition
  • Continental US Warranty 90 Days & 60 Days International.
  • Savings over $35,000
  • Preventative Maintenance (PM) & Calibration Performed.
  • Multiple Roche LightCycler 480-II units on hand

 

Our Advantage

  • We are passionate about lab equipment!
  • We offer detailed photos and videos of the instrument
  • Are an approved vendor to most large US and Canadian Universities & Colleges
  • Are an approved vendor to many US and International Biopharma companies 
  • We carry hundreds of advanced research equipment in stock 
  • Thousands of upgrade, modification, and repair parts are in stock for our customers’ instruments.
  • Remote installation and instrument operation training packages
  • International shipping is no problem

 

Our Guarantee

  • In Stock, Multiple LightCycler 480-II systems on hand if you require more than one
  • The LightCycler comes with parts and a labor 90 Days Warranty
  • Remote installation and training options
  • Smooth process from the initial quote to custom order fulfillment
  • Customer references at many universities

 

Roche LightCycler 480-II Overview 

 

Grade:

  • Research Grade Real-Time PCR Machine

 

The LightCycler 480-II by Roche:

The Roche LightCycler 480-II is a versatile real-time PCR system renowned for its high-throughput capacity, making it ideal for both research and clinical diagnostics. This system excels in its ability to handle a variety of applications, from gene expression analysis to genotyping. Its multicolor detection enables multiplexing, thus facilitating simultaneous detection of multiple targets in a single reaction. With user-friendly software, the capacity for automation, and compatibility with both 96-well and 384-well plates, it offers flexibility and efficiency to laboratories. Furthermore, its closed-system design minimizes contamination risks, and its integration with Roche ensures reliable support and training for users.
 

      

Assay/Application examples:  

  • Quantitative PCR (qPCR)
  • High-Resolution Melting (HRM) Analysis
  • Gene Expression Analysis
  • Genotyping 
  • Viral Load Testing 
  • Pathogen Detection
  • Mutation Scanning
  • Copy Number Variation (CNV) Analysis
  • Protein-DNA Interaction Studies (e.g., ChIP-qPCR)
  • Methylation Analysis

 

The Roche LightCycler 480-II is a real-time polymerase chain reaction (PCR) system used for the amplification, detection, and quantification of nucleic acid sequences with several key features:

 

  • High-throughput: Designed for a high sample volume, allowing for increased throughput.
  • Versatile: Supports various molecular biology applications.
  • Accurate and Precise: Offers reliable results.
  • Multicolor Detection: Allows multiplexing with multiple fluorophores.
  • User-friendly: Intuitive software for setup and analysis.
  • Automation Ready: Compatible with robotic systems.
  • Scalable: Handles 96-well and 384-well plates.
  • Reliable Support: From a reputable company.
  • High-Resolution Melting: For genotyping and mutation scanning.

 

These features combined make the Roche LightCycler 480-II an effective tool for laboratories requiring high-throughput and high-quality PCR Results.

 

Specifications: 

Block: 384-well Silver Thermal Block

Capacity: 384-well Plate with installed 384-silver block (Swap to 96 well block or extra 96 well blocks are available)
Temp Range: 37 - 95C
Accuracy: +/-0.2C of target temperature
96-well block: 4.8C/s
Dimensions (WxDxH): 57.4 x 58.8 x 49.7cm

Detector: Cooled monochrome CCD camera.
Resolution: 1024 x 1344 pixel
Integration time: 10 ms to 10 s
Integration time selection: Dynamic or Manual
Sensitivity: < 0.2 nmol/l fluorescein, typically 0.1 nmol/l
(20ul reaction volume)
Reproducibility: CV =< 0.15%
Crosstalk well-to-well: < 0.2% optically
< 0.02% with software correction

 

Filter Set of the LightCycler 480 II Instrument

 

Excitation wavelengths (nm):
Bandpass Half Band Width (HBW)
440nm 35nm
465nm 25nm
498nm 40nm
533nm 25nm
618nm 35nm

 

Detection wavelengths (nm):
Bandpass Half Band Width (HBW)
488nm 20nm
510nm 20nm
580nm 20nm
610nm 20nm
640nm 20nm
660nm 95nm(low pass)

 

This is the Roche LightCycler 480 II will include the following: 

Roche LightCycler 480 II Thermal Cycler
384 silver thermal cycler block
384-well block cycler cover
Computer running LightCycler software
Communication and power cables

 

BrochureRoche LightCycler 480-II PCR

ManualRoche LightCycler 480-II PCR

 

COMMON QUESTIONS AND ANSWERS BELOW

 

Steps you take to verify, service, and confirm the functionality of the Roche LightCycler 480-II?

Summary of testing & maintenance we perform (depending on included configuration):

  • We inspect the Roche LightCycler 480-II for physical damage.
  • The LightCycler 480-II system components are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary.
  • The proper operation of electronic components is confirmed
  • Necessary repairs and services performed to make sure the LightCycler 480-II will function as intended
  • We perform stress tests on the thermal block and electronics. 
  • We perform tests of all the instrument functions listed below
    • Self-check: PASSED
    • Check Lamp Stability: PASSED
    • Check Camera and Filter: PASSED
    • Perform Measurement Excitation testing: PASSED
    • Check Filter Wheel Response Time: PASSED
    • Check Filter Dark Position: PASSED
    • Check Filter Motor Connection: PASSED
    • Check Flat Field Parameters: PASSED
    • Check Cover Marker Coordinates: PASSED
    • Check Camera Hot Pixels: PASSED
    • Check Bias Value: PASSED
    • Check Noise: PASSED
    • Exchange Reference Channel: PASSED
    • Check Camera: PASSED
    • Check Cover Heater Temperature: PASSED
    • Tested barcode scanner: PASSED
    • X and Y-axis calibration: PASSED (unit is less than 5% of allowable offset)
    • Excitation Measurement and Detector alignment: PASSED
    • Blank plate run performed with no errors: PASSED

What supplies do I need?

You will need the consumables starting out: 

 

Can I view the Roche LightCycler 480-II in person at your facility or do a live Zoom demo before it is shipped to me?

We offer both onsite and remote demos via Zoom.

  • We invite you to come in for an onsite Demo or schedule a Zoom demo of the LightCycler 480-II.

 What about the warranty? Installation, training, maintenance?

Roche LightCycler 480-II Advanced PCR System comes with a 90-day Warranty.

 

Warranty Terms:

  • The warranty term is 90 Days from the date of the Roche LightCycler 480-II’s arrival at the destination.
  • This includes parts and labor.
  • The customer should retain all packing materials during the warranty period. 

 

Exceptions: 

  • Damage to the system due to the use of incorrect consumables.
  • Electrical damage from plugging into an incorrect voltage. 
  • Improper storage or transportation procedures.
  • Issues caused by lack of proper standard maintenance.
  • Moisture/Water damage.
  • Physical damage.

 

Optional Training Package:

We can offer Roche Instrument Training (Remote setup, general maintenance procedures guidance). This service is highly recommended to avoid a lot of the above issues. 

 

What should I watch out for when buying a Roche LightCycler 480-II system used/pre-owned from another lab?

Basic steps to confirm quality:

  • Confirm that all the Roche LightCycler 480-II covers are not cracked or broken. (It is very common for the Thermal Block door to be damaged, affecting the interlocks.)
  • Confirm the plate drawer opens and closes smoothly.
  • Confirm that the instrument is on a Windows 10 computer and communicates. 
  • Make sure the Thermal Block is in good working order. (They are thousands of dollars to replace.)  
  • Confirm that the Lamp is in good condition and the Xenon reflector lamp is new.

 

If you have concerns, we highly recommend inspecting the Roche LightCycler 480-II  in person or doing a live Zoom call; this will allow you to evaluate the condition.

 

 Do you have Roche LightCycler 480-II units on hand?
  • We stock multiple Roche LightCycler 480-II  systems along with many replacement parts to back our warranty.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get the Roche LightCycler 480-II  into your lab faster.
  • POs are accepted from US and Canadian universities.

 

 How fast will you ship?
  • We can ship within a few days to 2 weeks. Ship times depend on the current demand for our instruments. Members of our sales team will be able to offer current estimates.

 

Will my instrument be insured during shipping?
  • All Roche LightCycler 480-II are shipped fully insured within the US and internationally as long as we are a shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

 How do you pack? Have you packed and shipped LightCyclers in the past? Do you ship Internationally?

Packing

  • Most Roche LightCycler 480-II systems ship in fully enclosed ISPM-15 certified wooden crates, with proper padding underneath and the sides, and have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally we will only ship via Air Freight or Air Parcel (such as UPS, DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience shipping robotic liquid handelers around the world.

Our experience and process

  • We have safely packed and shipped hundreds of sensitive analytical instruments, including Roche LightCycler 480-II.
  • Before final packing/shipping, the Roche LightCycler 480-II is re-checked to verify performance.

 

*SM* Small Crate
275#

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is certainly worth it, and here is why.  Feel free to ask for feedback from hundreds of customers who bought from us.

  • You will save around 55-70% vs. buying a new Roche.  Why not spend leftover funds on another instrument for your lab?
  • You will get a 90-day warranty, plus, when available, an option to obtain an extended warranty agreement. 
  • Get peace of mind knowing that your instrument was tested and working the day it left our facility and is covered if there are faults.
  • Many customers choose to invest funds into Extended Warranties or maintenance plans for additional piece of mind.

 

The Roche LightCycler 480-II could be priced up to $65,000. So why not save your funds by buying Roche LightCycler 480-II used, get the high-quality sample preparation you expect from Roche, and use your leftover funds elsewhere?

 Sustainability
  • Well, this one is easy; buying a pre-owned Roche LightCycler 480-II is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.   

 I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-366-2699) or complete this form to get your questions answered or receive a formal Quote.

 

 

All equipment warranty request must have a support case opened within the 60-day warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.

Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies