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CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color

CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
CYTEK Millipore Guava EasyCyte 5HT HT Flow Cytometer 1 Laser 3 Color
Most flow cytometers ship within 5-7 days
Multiple Easycyte Flow Cytometers Sold in the last 12 months
Happy to provide Customer references available upon request
Extended Warranty and Setup Traning Available

 

  • Stock: In Stock
  • Brand: Guava
  • Product Code: SM100141-1
  • Condition: Pre-Owned
$10,995.00
CLICKABLE TABS BELOW

CYTEK Millipore Guava EasyCyte Cytometer 1 Laser 3 Color

 

  • Fully Functional
  • Fully Serviced 
  • Good condition
  • Optics Cleaned
  • Continental US Warranty 90 Days & 60 Days International.
  • Savings between $20,000 and $40,000
  • Multiple EasyCyte Cytometers on hand

 

 

Optional Training:

We can offer EasyCyte Cytometer training (Remote setup, general software usage, and maintenance procedures guidance)

 

CYTEK/Millipore Guava EasyCyte 1 Laser 3 Color Cytometer Overview and Application

 

Grade:

Research Grade Flow Cytometer

The Guava EasyCyte is a versatile and user-friendly flow cytometer. It offers precise multidimensional analysis, cost-efficiency, and flexibility for different sample types. Unique features include bead-free absolute cell counting for greater accuracy and a safe, non-pressurized system.

 

Assay/Application examples: 

Protein Detection – bead-based immunoassay for flow cytometry that detects IgG, IgM, and IgA antibodies.

 

Cell Health or Viability – with the Guava ViaCount reagents, EasyCyte can distinguish viable, apoptotic, and dead cells.

 

Mitochondrial potential – Information on mitochondrial stress and early, mid, and late apoptosis.

 

Cell Pathways –  detect the extent of Histone H2A.X pathway activation by measuring H2A.X phosphorylation in any given cell population.

 

Autophagy – a quantitative approach to analyzing autophagy through flow cytometry, using EasyCyte specialized reagents to separate and protect LC3 for accurate quantification.

 

Cell Cycle – simplifying cell cycle phase measurements using propidium iodide staining, Guava EasyCyte software offers quick assessments and automatic data export, ideal for researchers studying mitosis or cancer therapeutics.

 

CYTEK Guava EasyCyte Flow Cytometer Configuration:

Operating Environment

Temperature: 16°–30°C (60°–86°F)
Humidity: 10–90% relative humidity (non-condensing)
Power: 100–240 VAC, 50/60 Hz, 120W
Fuse Rating: 2.5 A (110 V, USA, and Japan), 1.6 A (220–240 V, Europe)

Dimensions and Weight: 25 cm (H), 51 cm (W), 61 cm (D); 31.8 kg weight

Optics

Laser: Blue 488 nm
Detectors: Forward Scatter and Side Scatter (Photodiodes), Fluorescence (see "easyCyte HT Lasers and Fluorescent Filters")

 

Performance

Counting Accuracy: ±10%
Counting Precision: ≤10% CV

 

Fluidics

Flow Cell Dimension: Square capillary, 100 μm ID
Pump: Positive displacement
Sample Flow Rate: 7 to 36 μL/min
Waste & Cleaning Vials: 30 mL glass vials with screw tops
Waste Generation: <40 mL over 8 hours of continuous use
Dead Volume: 50 μL (for 96-well microplate); 75 μL (for 0.5 mL microcentrifuge tubes)
Sample Concentration: 104 to 106 particles/mL for accurate results
Sample Requirement: Minimum 2,000 cells/test, typically 25,000-100,000 cells/test, depending on the assay

 

Channels/colors off the blue laser:

Green 525/30 (GRN-B) parameter – FITC, GFP, or Alexa Fluor® CF-488
Yellow 583/26 (YEL-B) parameter – Phycoerythrin (PE)-based, Cy3, Alexa Fluor® 532, propidium iodide (PI), TRITC, and DS Red reagents
Red 680/30 (RED-B) parameter – PE-Cy5, PerCP, 7-AAD, PE-Cy5.5, and PI

 

This CYTEK/Millipore Guava EasyCyte Cytometer will include the following:

Millipore Guava easyCyte HT Flow Cytometer

Installed easyCyte HT FlowCell (NEW)
Computer running guavaSoft
Communication and power cables

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What are the benefits of having a CYTEK Guava EasyCyte Cytometer in your lab?
 

The Guava EasyCyte is a high-performance benchtop flow cytometer designed for use in a wide range of applications, including immunology, cell biology, cancer research, and other fields of biomedical research. Here are some of the key benefits of owning a Guava:

 

  • Multidimensional Analysis: The system enables simultaneous measurement of multiple parameters, such as cell size, granularity, and different cell markers, allowing for sophisticated cell population analyses.
  • Sensitivity and Precision: The Guava EasyCyte provides high sensitivity and precision, which is crucial for detecting subtle changes in cell populations. This level of detail can be critical in fields such as cancer research, where detecting small populations of abnormal cells can be crucial.
  • Ease of Use: This system is designed to be user-friendly, with intuitive software and a compact, benchtop design. This can save users time and reduce the learning curve for new operators.
  • Absolute Cell Counting: Unlike traditional flow cytometers, the Guava systems can perform absolute cell counts without the need for additional reference beads, simplifying the procedure and providing more accurate results.
  • Cost-Effective: The Guava EasyCyte can analyze individual cells in a sample, allowing for more efficient use of samples and reagents. This can help to keep research costs down.
  • Absolute Cell Counting: Unlike traditional flow cytometers, the Guava systems can perform absolute cell counts without the need for additional reference beads, simplifying the procedure and providing more accurate results.

Steps you take to verify, service, and confirm the functionality of the CYTEK Guava EasyCyte Flow Cytometer?

Summary of testing & maintenance we perform (depending on included configuration):

  • We inspect the EasyCyte for physical damage.
  • The cytometer optics are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary
  • The proper operation of electronic components is confirmed
  • Necessary repairs and services are performed to make sure the cytometer will function as intended.
  • We measure the lasers' power output and ensure it is not degraded. 
  • We clean the fluidics and optical paths of the cytometer.
  • Preventative maintenance is performed as part of this process.
  • PMTs are tuned.
  • We run calibration particles and ensure that each channel provides clean signals with the required sensitivity and intensity.
  • EasyCyte HT Flow cell storage procedure is performed, thus ensuring the instrument will function well once used by the customer.

What supplies do I need?

You will need the following consumables starting out: 

 

Can I view the cytometer in person at your facility or do a live Zoom demo before it is shipped to me?

We offer both onsite and remote demos via Zoom.

  • We invite you to come in for an onsite Demo or schedule a Zoom demo of the cytometer.

 What about the warranty? Installiation, training, maintenance?

Our CYTEK Guava EasyCyte Cytometer comes with a 90-Day Warranty.

 

Warranty Terms:

  • The warranty term is 90 Days from the date of the cytometer's arrival at the destination.
  • This includes parts and labor.

 

Exceptions: 

  • The warranty does not cover clogs.
  • Shipping damage that is not reported at the time of delivery.
  • Damage to the system due to the use of incorrect fluids or samples.
  • Electrical damage from plugging into an incorrect voltage. 
  • Improper storage procedures that will allow the FlowCell to dry out improperly.
  • Issues caused by lack of proper standard maintenance.
  • Water damage.
  • Physical damage.

 

Optional Training Package:

We can offer EasyCyte Cytometer training (Remote setup, general software usage, and maintenance procedures guidance). This service is highly recommended to avoid a lot of the above issues. 

 

What should I watch out for when buying a CYTEK Millipore Guava EasyCyte Cytometer used/pre-owned from another lab?

Basic steps to confirm quality:

  • Confirm that internal optics are clean; this is often a complex undertaking if not already done.
  • Confirm there is no present physical damage to the cytometer.
  • Confirm that each channel provides clean signals with the required sensitivity and intensity.
  • Make sure there are no loose/damaged pumps that may cause leaks.
  • Make sure the fluidics system is not clogged and all valves operate reliably. (a major concern if the shutdown procedure was not followed)  
  • Ensure the sample tray does not skip steps, is not bent, and moves freely on all axis.
  • Ensure the lasers are up to specification and PMT voltages are properly adjusted. (These are not user adjustable)
  • Confirm that the cytometer is appropriate to your application; 
  • Confirm that the cytometer is tested with calibration particles and gives good data.
  • Make sure there are no missing components; it won`t be fast or inexpensive to correct.

 

If you have concerns, we highly recommend inspecting the cytometer in person or doing a live Zoom call; this will allow you to evaluate the cytometer and confirm that everything works.

 

 Do you have CYTEK Guava EasyCyte cytometers on hand?
  • We stock multiple CYTEK Guava EasyCyte Cytometers along with many replacement parts.

 

I am from the University or College. Do you accept Purchase Orders?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get the cytometer into your lab faster.
  • POs are accepted from US and Canadian universities.

 

 How fast will you ship?
  • We can ship within a few days to 2 weeks. Ship times depend on the current demand for our instruments. Members of our sales team will be able to offer current estimates.

 

Will my instrument be insured during shipping?
  • All CYTEK Guava EasyCyte cytometers are shipped fully insured within the US and internationally as long as we are a shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

 How do you pack? Have you packed and shipped cytometers in the past? Do you ship Internationally?

Packing

  • Most cytometers ship in fully enclosed ISPM-15 certified wooden crates, with proper padding underneath and the sides, and have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries or smaller cytometers to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally we will only ship via Air Freight or Air Parcel (such as UPS, DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience shipping cytometers around the world.

Our experience and process

  • We have safely packed and shipped hundreds of sensitive analytical instruments, including cytometers.
  • Before final packing/shipping, the cytometer is re-checked using calibration particles to verify performance.

 

*SM* Small Crate
270#

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is certainly worth it, and here is why.  Feel free to ask for feedback from hundreds of customers that bought from us.

  • You will save around 55-70% vs. buying a new cytometer.  Why not spend leftover funds on another instrument for your lab?
  • You will get a 90 days warranty, plus, when available, an option to obtain an extended warranty agreement. 
  • Get peace of mind knowing that your instrument was tested and working the day it left our facility and is covered if there are faults.

 

The CYTEK Guava EasyCyte could be priced up to $55,000. So why not save your funds by buying a Good, Tested, Used CYTEK Guava EasyCyte, get the high-quality data quality you expect from EasyCyte, and use your leftover funds elsewhere?

 Sustainability
  • Well, this one is easy; buying a pre-owned Guava EasyCyte is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.   

 I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-366-2699) or complete this form to get your questions answered or receive a formal Quote.

 

 

 

 

 

 

 

 

 

 

 

 



 

Luminex Millipore Guava EasyCyte 5HT Cytometer Brochure

 

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Luminex Millipore Guava EasyCyte 5HT Cytometer Brochure

 

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Every instrument has unique packing and shipping needs. Few instruments can be safely shipped via Pacel, and most require the safety of a well-built crate.

Here we are showing you our standard packing process  

      1. All items are different, but they all follow the same principle when packaging.

      2. Start with a well-constructed crate

A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.

      3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.

      4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.

      5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.

      6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.

      7. Final step is to photograph everything inside the crate and then seal it with screws.

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies