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- Stock: SOLD
- Brand: ProteinSimple
- Product Code: 54033
- Condition: Pre-Owned
For Sale Bio-Techne Protein Simple Wes Western Blot System
Pre-owned instrument
- Fully Functional
- Serviced & in Stock
- Good cosmetic condition
- Warranty
The predecessor to the ProteinSimple Jess Simple Western System
Here is what you will receive:
- Protein Simple Wes Western Blot system
- Computer running Compass for SW
- Monitor, keyboard, and mouse
- Power and communication cables
Please note: the cartridge is not included.
Protein Simple Wes Western Blot System Configuration:
- Throughput: up to 25 samples in 3 hours
- Accuracy: separate and analyze proteins by size from 2-440 kDa
- pg-Level sensitivity: with just 3uL
- Dimensions(WxDxH): 33 x 52 x 33cm
Total Protein Specification:
- Sample Required: 0.3 - 1.2ug lysate
- Volume Required: 3 uL/well
- Size Range: Molecular weight (MW) ladder ranges from 2 - 440kDa
- Sizing CV: <10%
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Resolution (+/- percent difference in MW): +/- 15-20% for MW <20 kDa / +/- 10% for MW >20 kDa
- Quantitation CV: <20%
- Dynamic Range: 2-3 logs
- Sensitivity: ng
- Capillary: 5cm, 100um, 400nL
Immunoassay Charge Specification:
- Sample Required: 0.6 - 1.2ug
- Volume Required: 3 uL/well
- Size Range: Molecular weight (MW) ladder ranges from 2-440kDa
- Sizing CV: <10%
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Resolution (+/- percent difference in MW): +/- 15-20% for MW <20 kDa / +/- 10% for MW >20 kDa
- Quantitation CV: <20%
- Dynamic Range: 3 logs
- Sensitivity: Low pg
- Capillary: 5cm, 100um, 400nL
Customization/Options: N/A
Testing performed by our in-house service team:
The Protein Simple Wes Western Blot System passed all tests and was found to be fully functional:
- We powered it on
- We conducted initialization and homing
- We checked communication with the software
- We ran the self-tests using a test cartridge (results are saved to the desktop on the computer)
- vacuum leak: PASSED
- unregulated vacuum level: PASSED
- fluor LED detection: PASSED
- amber LED detection: PASSED
- Blue LED detection: PASSED
- white LED detection: PASSED
- light leak detection: PASSED
- lamp motor: PASSED
- camera: PASSED
- dark masters: PASSED
- door motor: PASSED
- EE voltage: PASSED
- temp sensors: PASSED
- lamp on: PASSED
- tray jog: PASSED
- tray move: PASSED
- tray home: PASSED
- Z-stage jog: PASSED
- Z-stage move: PASSED
- Z-stage home: PASSED
- disk storage: PASSED.
The self-test for this instrument is very intensive and covers every moving part: electronics, camera, sensors, lights, and vacuum system. Due to this, you can purchase this Protein Simple Wes Western Blot System knowing it is ready to go.
Instrument Overview:
- The Protein Simple Wes Western Blot System automates protein separation and analysis.
- It offers high sensitivity and reproducibility for western blotting experiments.
- With minimal hands-on time, it accelerates protein analysis workflows.
- The Wes system requires small sample amounts, conserving precious samples.
- It enables quantitative analysis of protein expression and post-translational modifications.
- This system simplifies western blotting, delivering reliable and consistent results.
Instrument Application:
- Simple Western-size assays
- Chemiluminescence
- Protein expression analysis
- Post-translational modification studies
- Biomarker discovery
- Drug Development
The key features:
- Automated western blotting with Protein Simple Wes System.
- Simplified protein analysis workflow.
- Precise quantification and reproducibility.
- Rapid results in 3-4 hours.
- Minimal hands-on time.
- High sensitivity and accuracy.
Additional technical info:
- Buffer Compatibility: Protein Simple Wes Western Blot System Buffer Compatibility
- Manual: Protein Simple Wes Western Blot System Manual
- Installation Guide: Protein Simple Wes Western Blot System Installation Guide
Video Overview:
About us
How we pack
Why should you buy from us?
- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping.
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Many upgrade and repair parts are in stock.
- Remote installation and instrument operation training are available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
- It will work!
- Warranty
- You get after-sales technical support.
- Smooth process from the initial quote to order fulfillment.
- Customer references are available.
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*SM* Crate
225#
COMMON QUESTIONS AND ANSWERS BELOW
What steps do you take to verify, service, and confirm the functionality of the Protein Simple Wes Western Blot System?
We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.
- We powered it on
- We conducted initialization and homing
- We checked communication with the software
- We ran the self-tests using a test cartridge (results are saved to the desktop on the computer)
- vacuum leak: PASSED
- unregulated vacuum level: PASSED
- fluor LED detection: PASSED
- amber LED detection: PASSED
- Blue LED detection: PASSED
- white LED detection: PASSED
- light leak detection: PASSED
- lamp motor: PASSED
- camera: PASSED
- dark masters: PASSED
- door motor: PASSED
- EE voltage: PASSED
- temp sensors: PASSED
- lamp on: PASSED
- tray jog: PASSED
- tray move: PASSED
- tray home: PASSED
- Z-stage jog: PASSED
- Z-stage move: PASSED
- Z-stage home: PASSED
- disk storage: PASSED.
What supplies do I need?
You will need the consumables starting out: (Here are some helpful links)
- Simple Western Antibody Database (bio-techne.com)
- Simple Western Assay Modules (bio-techne.com)
- Simple Western Consumables (bio-techne.com)
- Simple Western Individual Charge Reagents (bio-techne.com)
- Simple Western Kit Builder (bio-techne.com)
Can I view the Protein Simple Wes Western Blot System in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Protein Simple Wes Western Blot System used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We powered it on
- We conducted initialization and homing
- We checked communication with software
- We successfully carried out self-test
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this Protein Simple Wes Western Blot System elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We must purchase any required or missing accessories to complete the instruments.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing and shipping sensitive instruments.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you previously packed and shipped Protein Simple Wes Western Blot System? Do you ship Internationally?
Packing
- Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot, and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.