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BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS

BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS
Approved Vendor to many US, Canada, and EU Universities
International Shipping
Customer References Available 
Training and Installation Options Available

 

  • Stock: In Stock
  • Brand: BD
  • Product Code: 50165
  • Condition: Pre-Owned
$52,995.00
CLICKABLE TABS BELOW

For Sale BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors w/ HTS


Pre-owned instrument

  • Fully Functional
  • Current model
  • Serviced & in Stock
  • Good cosmetic condition
  • Warranty
  • An extended warranty option and training may be available.

 

Here is what you will receive:

  • BD LSRFortessa Flow Cytometer
  • HTS High Throughput Sampler
  • Computer with FACSDiva software ver9.0 w/ License Dongle (w/ monitor, keyboard, mouse)
  • Communication and power cables.

 

BD LSRFortessa X-20 Cell Analyzer Flow Cytometer Configuration:

  • Optics: (4) Lasers: Violet (405nm), Blue (488nm), Yellow/ Green (561nm), Red (650nm), (16) Colors simultaneously, (18) Detector Channels
  • Forward Scatter Detection: Photodiode detector with a 488/10 BP filter
  • Side Scatter Detector: Photomultiplier tube (PMT) with a 488/10 BP filter
  • Lasers: 405 violet laser detection channels: A (780/60, 750LP), B (710/50, 690LP), C (670/30, 635LP), D (610/20, 600LP), E (525/50, 505LP) F (450/50, GG420); 488 blue laser detection channels: A (710/50, 685LP), B (530/30, 505LP); 561 yelllow/green laser detection channels: A (780/40, 750LP), B (710/50, 685LP), C (670/30, 635LP), D (610/20, 600LP), E (586/15); 650 red laser detection channels: A (780/60, 750LP) B (730/45, 690LP), C (670/30, RG665)
  • Fluorescence Sensitivity: FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC), PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE), PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5), APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
  • Fluorescence Resolution: Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Fluorescence Linearity: Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
  • Data Acquisition Rate: 40,000 events/sec with beads
  • Fluidics: Sample Flow Rates - Front button panel provides three modes: RUN, STANDBY, and PRIME; Continuously adjustable flow rate, plus three preset flow rates: LO: 12 uL/min; MED: 35 uL/min; HI: 60 uL/min
  • Dimensions (H x W x D): 76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.)
  • Weight: ~159 kg (~350 lb)
  • High Throughput Sampler: The BD High Throughput Sampler (HTS) is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate; HTS Throughput Acquisition: less than 15 minutes per microtiter plate in high-throughput mode using a 2-second acquisition, less than 44 minutes in standard mode using a 10-second acquisition; Carryover: <0.5% High-throughput mode; <0.75% Standard mode

 

Customization/Options:

N/A

 

Testing performed by our in-house service team:

The BD LSRFortessa X-20 Cell Analyzer Flow Cytometer passed all tests and was found to be fully functional:

  • We tested it for connection to software (Diva 9)
  • We tested it for fluidics priming and sequences.
  • We tested it for several cleaning cycles (long clean, flow cell clean, etc.)
  • We tested it for HTS functionality.
  • We tested it for Optical Alignment.
  • We tested it for Homing, Priming, and Cleaning.
  • We tested it for a run with 8 Peak Beads.

 

Instrument Overview:

  • The BD LSRFortessa X-20 Cell Analyzer Flow Cytometer is a cutting-edge instrument designed for high-throughput flow cytometry analysis.
  • It boasts advanced optical configurations, enabling multi-parameter detection with high sensitivity and resolution.
  • Equipped with multiple lasers and detectors, it offers versatility in analyzing diverse cell populations and fluorescent markers.
  • Its intuitive software interface facilitates seamless data acquisition, analysis, and visualization.
  • With robust automation features and a user-friendly design, it optimizes workflow efficiency and productivity.
  • Overall, the LSRFortessa X-20 is a powerful tool for research in immunology, oncology, and stem cell biology.

 

Instrument Application:

  • The BD LSRFortessa X-20 Cell Analyzer Flow Cytometer is widely applied in immunology research for studying immune cell populations and their functions.
  • Researchers use it to analyze cell signaling pathways, immune response dynamics, and cytokine production profiles.
  • In oncology research, it aids in characterizing cancer cell populations, assessing tumor heterogeneity, and studying drug responses.
  • Additionally, it finds application in stem cell biology for evaluating differentiation processes, cell cycle dynamics, and pluripotency markers.
  • The cytometer's high-throughput capabilities make it suitable for large-scale screening studies and clinical research applications.
  • Overall, the LSRFortessa X-20 enhances understanding of cellular processes and contributes to advancements in various fields of life sciences.

 

The key features:

  • Multicolor analysis.
  • High-throughput capability.
  • Precise cell counting.
  • Robust optical design.
  • Automated data acquisition.
  • Comprehensive software interface.

 

Additional technical info:

 

Video Overview:

 

About us

 

How we pack

 

Why should you buy from us?

 

  • We have experience servicing and repairing these instruments.
  • This instrument goes through all the described service steps before shipping.
  • We offer detailed photos and videos of the instruments you buy.
  • We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
  • We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
  • Many upgrade and repair parts are in stock.
  • Remote installation and instrument operation training are available.
  • On-site installation may be available in the Boston Area (New England area)
  • US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

 

  • It will work!
  • Warranty
  • An extended warranty may be available.
  • You get after-sales technical support.
  • Smooth process from the initial quote to order fulfillment.
  • Customer references are available.

 

*LO* Long Crate ESQ
750#

 

COMMON QUESTIONS AND ANSWERS BELOW

 

What steps do you take to verify, service, and confirm the functionality of the BD LSRFortessa X-20 Cell Analyzer Flow Cytometer?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

  • We tested it for connection to software (Diva 9)
  • We tested it for fluidics priming and sequences.
  • We tested it for several cleaning cycles (long clean, flow cell clean, etc.)
  • We tested it for HTS functionality.
  • We tested it for Optical Alignment.
  • We tested it for Homing, Priming, and Cleaning.
  • We tested it for a run with 8 Peak Beads.

 

What supplies do I need?

You will need the consumables starting out:

 

Can I view the BD LSRFortessa X-20 Cell Analyzer Flow Cytometer in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.

 

What about the warranty? Installation, training, maintenance?

Warranty Terms:

  • The instrument comes fully warranted for both parts and labor.
  • The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a BD LSRFortessa X-20 Cell Analyzer Flow Cytometer used/pre-owned from another lab?
  • Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
  • Confirm that there is no physical damage or any misaligned internal components.
  • Ask what accessories are included.
  • Ask how long the warranty is and what it covers.

 

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

 

  • We tested it for connection to software (Diva 9)
  • We tested it for fluidics priming and sequences.
  • We tested it for several cleaning cycles (long clean, flow cell clean, etc.)
  • We tested it for HTS functionality.
  • We tested it for Optical Alignment.
  • We tested it for Homing, Priming, and Cleaning.
  • We tested it for a run with 8 Peak Beads.

 

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.

 

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this BD LSRFortessa X-20 Cell Analyzer Flow Cytometer elsewhere, possibly for less.

 

Here are some of the reasons for our price.

  • We may offer longer warranty terms.
  • We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
  • We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
  • We invest considerable time into the testing and service of each instrument we sell.
  • We have to purchase various testing supplies (often expensive) to perform testing.
  • We must purchase any required or missing accessories to complete the instruments.
  • We have to procure and stock service parts to support our warranty.
  • We have extensive experience packing and shipping sensitive instruments.
  • We guarantee that the instrument will be functional.

 

This procurement is for a University or College. Are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
  • POs are accepted from US and Canadian universities.

 

How fast will you ship?
  • We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

 

Will my instrument be insured during shipping?
  • All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
  • If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

 

How do you pack? Have you previously packed and shipped BD LSRFortessa X-20 Cell Analyzer Flow Cytometer? Do you ship Internationally?

Packing

  • Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
  • Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

  • We have safely packed and shipped thousands of sensitive analytical instruments.

 

How much will I save buying it pre-owned, and is it worth it?

A lot, and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

 

  • You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
  • You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
  • Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
  • Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
  • Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.

 

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

 

 

Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Warranty
60-days
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies