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BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors w/ BD FACSFlow Supply System and High Throughput Sampler - AV

BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors w/ BD FACSFlow Supply System and High Throughput Sampler - AV
BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors w/ BD FACSFlow Supply System and High Throughput Sampler - AV

We apologize this Instrument is currently 
out of stock

Please submit a request, we will notify you if another one comes in stock. Thank you!

Approved Vendor to many US, Canada, and EU Universities
US & International Shipping



  • Stock: SOLD
  • Brand: BD
  • Product Code: 50956
  • Condition: Pre-Owned

BD FACSymphony A3 Flow Cytometer (3)Lasers/(14)Colors/(16)Detectors with BD FACSFlow Supply System and High Throughput Sampler

Pre-owned instrument

• Fully Functional
• Serviced & in Stock
• Current model
• Good cosmetic condition
• Extended Collection Bed Upgrade
Continental US Warranty 120 Days & 60 Days International.
• Options for Extended warranty, Installation and Training may be available

Here is what you will receive:
•BD FACSymphony A3 Flow Cytometer (Made in 2021.  New around $300,000.00)
• Power Conditioner
• Fluidics Supply Station
• Fluidics hoses
• Pressure Vessel with holder
• Waste container
• HTS system
• Desktop PC running BD FACSDiva software ver9.1 (w/ monitor, keyboard, mouse)
• Communication and power cables
• FCS Express (1-Year Subscription) RUO version

BD FACSymphony A3 Flow Cytometer System Configuration: 
Flow Cytometer:
• Optics:(3) Lasers, (14) Colors, (16) Detectors:
• Forward scatter detector: Photodiode with a 488/10 bandpass (BP) filter for the 488-nm laser
• Side scatter detector: PMT with a 488/10 BP filter for the 488-nm laser
• 488nm blue laser: 780/60, 710/50, 610/20, 575/25, 530/30
• 405nm violet laser: 780/60, 710/50, 670/30, 610/20, 470/15, 431/28
• 637nm red laser: 780/60, 710/50, 670/30

• Fluorescence sensitivity: may vary dependent on the combination of laser and filter selections
• Fluorescence resolution: coefficient of variation (CV)
• PI: Area, <3.0%, full G0 /G1 peak for PI-stained CEN
• Fluorescence linearity: doublet/singlet ratio: PI-stained CEN: 1.95–2.05 (488-nm laser)
• FSS sensitivity: sensitivity enables separation of fixed platelets from noise
• FSS resolution: scatter performance is optimized for resolving lymphocytes, monocytes and granulocytes
• SS resolution: scatter performance enables separation of 0.5-um beads from noise
• Acquisition rate: 40,000 events/second with bead

• Sample flow rates three modes: RUN, STANDBY and PRIME.
• Continuously adjustable flow rate, plus three preset flow rates: LO: 12 uL/min; MED: 35 uL/min; HI: 60 uL/min

• Instrument dimensions (W x D x H) 83.8 x 76.2 x 80 cm (33 x 30 x 31.5 in)
• Weight 167.9 kg (379 lb)
• Power Operation at 100/115/230 VAC and 50 or 60 Hz

High Throughput Sampler:
• Automates and accelerates sample acquisition
• Compatible with 96- and 384-well plates
• <0.5% sample carryover in high-throughput mode

FACSFlow Supply System:
• Increases capacity and ease of use while maintaining a stable fluidics pressure
• Reduces daily maintenance by incorporating a 20-L BD FACSFlow Cubitainer

• Extended Warranty
• Installation
• Training

QC Testing performed by our in-house service team:
• The BD FACSymphony A3 Flow Cytometer was successfully powered on by our team, ensuring its readiness for operation.
• Communication between the cytometer and its operating software was established and verified by us, confirming seamless integration.
• Our team completed the fluidics priming and sequence operations, achieving optimal system preparation without issues.
• Comprehensive system cleaning, including long clean and flow cell clean, was meticulously performed by us, ensuring the system's cleanliness and functionality.
• The high-throughput ssampler (HTS) functionality underwent rigorous testing by our specialists, with all systems confirmed as fully operational.
• We conducted and successfully confirmed the processes of homing, priming, and cleaning, of the (HTS) maintaining the system's efficiency.
• Laser alignment was precisely performed by our team, with accuracy and alignment confirmed to meet operational standards.
• Verification of all channels, using test beads, was conducted by us, ensuring each channel's functionality and readiness for accurate analysis using CS&T beads.

Instrument Overview:
• The BD FACSymphony A3 is designed for high-throughput cell analysis, enhancing lab efficiency.
• Features advanced fluidics and optical systems for precise sample handling and analysis.
• Supports multi-parametric analysis with high sensitivity and specificity.
• Equipped with a user-friendly interface, simplifying complex experimental setups.
• The instrument's modular design allows for easy upgrades and customization.
• Offers robust data management and analysis tools, streamlining workflow.

Instrument Application:
• Immunophenotyping
• Cell Cycle Analysis
• Apoptosis Detection
• Intracellular Cytokine Staining
• Cancer Research
• Functional Assays
• Multiplex Assays
• Receptor Occupancy
• Microbial Studies

• Datasheet: BD FACSymphony A3 Flow Cytometer Datasheet
• Brochure: BD FACSymphony A3 Flow Cytometer Brochure
• Manual: BD FACSymphony A3 Flow Cytometer Manual

Note: Subscription is for the Research Use Only (RUO) version. 
Video Overview:

Why should you buy from us?

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and instrument operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

It will work!
• Continental US Warranty 120 Days & 60 Days International.
• An extended warranty may be available.
• You get after-sales technical support.

• Smooth process from the initial quote to order fulfillment.
• Customer references are available.

This will ship via freight. Please contact us with your zip code to obtain shipping quote.
*LA* Large Crate ESQ

Please do not hesitate to contact us if you have any questions.



We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend thousands of dollars and receive a damaged instrument.

Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing.

We generally ship parcels either via UPS or freight for all larger goods.

Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant).

On freight shipments, we use 2” 1.7 lb. density foam, which provides excellent cushioning and support for the instrument.

Instruments are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provides cushioning in all directions and holds instruments firmly in place.

Final step is to photograph everything inside the crate and then seal it with screws.



Safe Shipping
From Small Packages to Large Freight
International Shipping
Happy customers all around the world
Registered Vendor To
Many US Colleges, Universities and Fortune 500 Companies