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BD Accuri C6 Plus Flow Cytometer 2 Laser 4 Color

BD Accuri C6 Plus Flow Cytometer 2 Laser 4 Color
BD Accuri C6 Plus Flow Cytometer 2 Laser 4 Color
Most flow cytometers ship within 5-7 days
Multiple Accuri Flow Cytometers Sold in the last 12 months
Happy to provide Customer references available upon request
Extended Warranty and Setup Traning Available


  • Stock: In Stock
  • Brand: BD
  • Product Code: GLFC100052
  • Condition: Pre-Owned

BD Accuri C6 Plus Flow 4 Color Cytometer


Current Model


  • Fully Functional
  • Fully Serviced 
  • Good condition
  • Optics Cleaned
  • Warranty 120 Days
  • Savings between $20,000 and $40,000
  • Multiple BD Accuri C6 Plus Flow Two Laser / Four Color Cytometers on hand


Optional Training and Extended Warranty:

  • We offer BD Accuri training (Remote setup, general software usage and maintenance procedures guidance)
    Extended warranty option available


BD Accuri C6 Plus Flow 4 Color Cytometer Overview and Application



Research Grade Flow Cytometer


Tuning Detector voltages

The BD Accuri C6 Plus cytometer is set up with standardized fluorescence performance; this means you do not need to adjust detector voltages. With a dynamic range of 7.2 decades, you will have 16 million channels of digital data being recorded, giving you flexibility in your gating strategies because all data is available all the time.


Assay/Application examples: 

  • Apoptosis detection 
  • Gap-free analysis of intracellular calcium 
  • Pluripotent stem cell analysis 
  • Gene expression analysis 
  • Analysis of DNA content and ploidy 
  • Analysis of marine and freshwater samples 
  • Analysis of aquatic microorganisms


BD Accuri 4 Color Flow Cytometer Configuration:

Cytometer Laser setup: This cytometer comes with blue and red lasers (Is a 2-laser system)
Detection Channels: Two light scatter detectors and four fluorescence detectors
Optical filters:4 Colors optimized for detecting FITC, PE, PerCP, and APC. (FL1: 533/30nm FL2: 585/40nm FL3: 670nm LP FL4: 675/25nm)


Common compatible Fluorochromes/Dyes per channel:

FL1 (533/30nm): FITC, BD Horizon Brilliant Blue 515, Alexa Fluor 488, CFSE, GFP, YFP
FL2 (585/40nm): PE, PI, PE-CF594
FL3 (670nm LP): PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy7, PI, 7-AAD
FL4 (675/25nm): APC, Alexa Fluor 647



Flow Rates and Core Sizes: 10-100 uL/min, 5-40 uM core
Minimum Detectable Particle Size: 0.5um
Scatter Resolution: Resolves human peripheral blood lymphocytes, monocytes, and granulocytes
Fluorescence Linearity: 2 +/-0.05% for chicken erythrocyte nuclei (CEN)
Fluorescence Precision: <3% CV for CEN
Flow Rates and Core Sizes: Slow 14 uL/min, 10 uM core, Medium 35 uL/min, 16 uM core, Fast 66 uL/min, 22 uM core, Custom 10-100 uL/min, 5-40 uM core
Minimum Detectable Particle Size: 0.5um
Minimum Sample Volume: 50uL
Flow Cell: 200um ID quartz capillary
Data Acquisition Rate: 10,000 events/second, maximum


This Accuri C6 Plus Flow Cytometer will include the following:

BD Accuri C6 Plus Flow Cytometer

Freshly Installed Maintenance kit
Fluid harness
Rack to organize the Bottles
Bottles set
Computer, mouse, keyboard, monitor, and cables
Communication and power cables


Optional Accessories :

A C-sampler for microplates or tubes can be added and allow for the following:

48 and 96 well microplates plates

Standard 12 x 75 mm tubes in a 24-tube rack




Steps you take to verify, service, and confirm the functionality of the BD Accuri C6 Plus Flow Cytometer?

Summary of testing & maintenance we perform (depending on included configuration):

  • We inspect the Accuri C6 Plus for physical damage.
  • The cytometer optics are inspected and cleaned.
  • Mechanical components inspected, adjusted, and lubricated if necessary
  • The proper operation of electronic components is confirmed
  • Necessary repairs and services performed to make sure the cytometer will function as intended
  • We measure the lasers' power output and ensure it is not degraded. 
  • We clean the fluidics and optical paths of the cytometer.
  • Preventative maintenance is performed as part of this process, and new fluid filters and pump tubing are installed.
  • The system is cleaned to less than ten events per second with DI water on the SIP.
  • We run calibration particles and ensure that each channel provides clean signals with the required sensitivity and intensity.
  • BD Accuri C6 Plus shutdown and storage procedure is performed, thus ensuring the instrument will function well once used by the customer.

What supplies do I need?

You will need the following consumables starting out: 


Can I view the cytometer in person at your facility or do a live Zoom demo before it is shipped to me?

We offer both onsite and remote demos via Zoom.

  • We invite you to come in for an onsite Demo or schedule a Zoom demo of the cytometer.

 What about the warranty?

Our BD Accuri C6 Plus Flow 4 Color Cytometers come with a 120-Day Warranty.


Warranty Terms:

  • The warranty term is 120 Days from the date of the cytometer's arrival at the destination.
  • This includes parts and labor.
  • During the first 60 Days, Boston Industries will also arrange and cover shipping expenses. 
  • During Days 61 thru 120 Days of the warranty period, the customer will be responsible for all shipping charges. 
  • The customer should retain all packing materials during the warranty period.



  • The warranty does not cover clogs.
  • Damage to the system due to the use of incorrect fluids or samples.
  • Electrical damage from plugging into an incorrect voltage. 
  • Improper storage procedures that will allow the cytometer to dry out.
  • Issues caused by lack of proper standard maintenance.
  • Water damage.
  • Physical damage.


 Do you offer installation, service, etc?
  • We offer installation and training packages.
  • We offer an extended warranty agreement on this model.
  • We stock various replacement and upgrade parts, so we may continue to support your cytometer.


What should I watch out for when buying a BD Accuri C6 Plus Flow Cytometer used/pre-owned from another lab?

Basic steps to confirm quality:

  • Confirm that internal optics are clean; this is often a complex undertaking if not already done.
  • Confirm there is no present physical damage to the cytometer.
  • Confirm that each channel provides clean signals with the required sensitivity and intensity.
  • Make sure there are no loose/damaged pumps that may cause leaks.
  • Make sure the fluidics system is not clogged and all valves operate reliably. (a concern if the shutdown procedure was not followed)  
  • If the cytometer has a C-Sampler, ensure it does not skip steps, is not bent, and moves freely on all axis.
  • Ensure filters are installed, inspected, and have no damage or dust inside.
  • Confirm that the cytometer is appropriate to your application; 
  • Confirm that the cytometer is tested with calibration particles and gives good data.
  • Make sure there are no missing components; it won`t be fast or inexpensive to correct.


If you have concerns, we highly recommend inspecting the cytometer in person or doing a live Zoom call; this will allow you to evaluate the cytometer and confirm that everything works.


 Do you have BD Accuri C6 Plus cytometers on hand?
  • We stock multiple BD C6 Plus Accuri Cytometers along with many replacement parts.


This procurement is for a University or College, are purchase orders with NET terms accepted?
  • If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get the cytometer into your lab faster.
  • POs are accepted from US and Canadian universities.


 How fast will you ship?
  • We can ship within a few days to 2 weeks. Ship times depend on the current demand for our instruments. Members of our sales team will be able to offer current estimates.


Will my instrument be insured during shipping?
  • All BD Accuri C6 Plus cytometers are shipped fully insured within the US and internationally as long as we are a shipper and handle shipping arrangements.
  • If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.


 How do you pack? Have you packed and shipped cytometers in the past? Do you ship Internationally?


  • Most cytometers ship in fully enclosed ISPM-15 certified wooden crates, with proper padding underneath and the sides, and have moving components braced.
  • Accessories are packed safely in boxes with padding and then safely packed inside the crate.
  • We may not use a crate for local deliveries or smaller cytometers to save you money *if delivered by us.
  • We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
  • Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

  • Within US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

  • Internationally we will only ship via Air Freight or Air Parcel (such as UPS, DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
    We have extensive experience shipping cytometers around the world.

Our experience and process

  • We have safely packed and shipped hundreds of sensitive analytical instruments, including cytometers.
  • Before final packing/shipping, the cytometer is re-checked using calibration particles to verify performance.


*SM* Small Crate

How much will I save buying it pre-owned, and is it worth it?

A lot…and it is certainly worth it, and here is why.  Feel free to ask for feedback from hundreds of customers that bought from us.

  • You will save around 55-70% vs. buying a new cytometer.  Why not spend leftover funds on another instrument for your lab?
  • You will get a 120 days warranty, plus, when available, an option to obtain an extended warranty agreement. 
  • Get peace of mind knowing that your instrument was tested and working the day it left our facility and is covered if there are faults.


BD Accuri C6 Plus Flow Cytometer is a predecessor model to the Accuri C6 Plus; The plus could be priced up to $55,000. So why not save your funds by buying Accuri C6 Plus Flow Cytometer, get the high-quality data quality you expect from BD, and use your leftover funds elsewhere?

  • Well, this one is easy; buying a pre-owned Accuri C6 Plus Flow Cytometer is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.   

 I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-366-2699) or complete this form to get your questions answered or receive a formal Quote.



We test every component in the BD Accuri C6 Flow Cytometer to ensure proper operation. We verify each component one by one and perform necessary services or maintenance to bring the instrument to our high quality standards and manufacture specifications.

  1. We inspect the Accuri C6 for physical damage.

  2. We inspect the cytometer for signs of electronic failure.

  3. We inspect for signs of previous services and assess if they meet our quality standards.

  4. We measure the lasers' power output to ensure they are not degraded. This tells us that they have plenty of life left in them.

  5. We operate and test flow through each of the fluidic valves.

  6. We test the operation of each of the pressure transducers.

  7. We replace worn items such as filters and tubing.

  8. We clean the fluidics and optical paths of the cytometer.

  9. We test communication and control with the BD Accuri C6 software.

  10. We verify that the software and computer operate optimally, protecting your precious data.

  11. We verify the system is clean to less than ten events per second with DI water on the SIP.

  12. We run calibration particles and ensure that each channel provides clean signals with excellent sensitivity and intensity.

  13. We perform proper BD Accuri C6 shutdown and storage procedures to ensure the instrument performs as expected when removed from storage.


BD Accuri C6 Flow Cytometer Users Guide


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BD Accuri C6 Plus Flow Cytometer Brochure


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BD Accuri C6 Plus Flow Cytometer Quick Reference Guide


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BD Accuri C6 Flow Cytometer Filter Guide


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