Zeiss Axiovert 200M Inverted Motorized Fluorescence DIC Microscope Pred.Observer

Product Code: 12785
Availability: 1
Price: $14,995.00

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Zeiss Axiovert 200M Inverted Motorized Fluorescence DIC Microscope Pred.Observer


Zeiss Axiovert 200M Inverted Motorized Fluorescence DIC Microscope Pred.Observer | Priced $14,995.00 | (12785)

Here is a Zeiss Axiovert 200M Inverted Motorized Microscope

Setup Brightfield with Phase Contrast, DIC and Fluorescence.

Predecessor to:
Axio Observer.

This Zeiss Axiovert 200M Inverted Motorized Microscope is in good working order with all aspects tested and working fully.
Microscope and optics have been professionally cleaned by our technician under a class 10,000 clean room hood.

Please see the exact video and photos below.

Testing we performed:
The Zeiss Axiovert 200M Inverted Motorized Microscope passed all tests and was found to be fully functional:
Tested Motorized XY Stage to limits
Tested Mercury lamp/source fully
Tested Halogen lamp/source fully
Tested port splitted between all 3 ports
Tested Z-axis adjustment and focus
Tested Filters and Prisms
Tested Connection between computer and camera
Tested Live Feed for clarity
Tested Eyepieces for clarity
Tested all light path components to be working fully
Tested control of all motorized components to be working fully

During testing this microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical and Motorized components moved freely and the test sample was able to be focused without excessive effort.

System consists of:
Zeiss Axiovert 200M Motorized Inverted Microscope
Binocular Tube Head
(2) E-Pi 10x/20 Eyepieces
Prisms / Condenser:
NA0.55 WD26mm Condenser
Prisms: Ph1, Ph2, Ph3, DIC
Analyzers / Polarizers:
Rotatable Polarizer D
Analyzer Filter Cube
HAL100 12V 100W Halogen Lamp Housing
E-Cite EXFO Metal Halide Light Guide & Power Supply
Foot Pedal Control for EXFO
Brightfield Path:
Transmitted Light Illumination Tower
Fluorescence Path:
Motorized Integrated Shutters
Fluorescence Turret / Cubes:
5-position Reflective Turret
DAPI EX:360/40nm DM: 400nm EM: 460/50nm
FITC EX: 480/40nm DM: 505nm EM: 535/50nm
CY3 EX: 545/30nm DM: 575nm EM: 610/75nm
Zeiss Analyzer Cube
ASI Motorized XY Stage
Intelligent Imaging Joystick MS-2000 Controller w/ power supply
6-position Motorized Turret
2.5x/0.075 Plan NEOFLUAR
5x/0.25 FLUAR
10x/0.50 FLUAR with 10x DIC prism
20x/0.50 Plan NEOFLUAR Ph2
Photo / Port Splits:
Left 100% or 60%Left 40% Head or Head 100%
AmScope MU300 3.1 MP CMOS Digital Camera
Motorized Components:
Objective Turret, Filter Cube Turret, Z-axis Focus, Halogen Lamp/Brightness, Fluorescence Shutter, Light path switching.
Computer and Software:
Dell Latitude i5 Laptop running Windows 10 Pro with AmScope and MicroManager Software

Communication and power cables.

Configuration and Setup:
Orientation: Inverted
Illumination Type: Brightfield with Phase Contrast, DIC & Fluorescence
Objectives: 2.5x/0.075 Plan NEOFLUAR, 5x/0.25 FLUAR, 10x/0.50 FLUAR, 20x/0.50 Plan NEOFLUAR Ph2
Light Sources: 12V 100W Halogen Lamp, 200W Mercury Arc
Stage Type: Motorized XY Stage

This Zeiss Axiovert 200M Inverted Motorized Microscope is currently set up for Brightfield with Phase Contrast, DIC and Fluorescence studies.
The AmScope CMOS digital colored camera is capable of taking detailed digital images.
The Zeiss system includes a Dell Latitude i5 laptop to control the AmScope software.
This includes control of all motorized components such as the Objective turret, filter cube turret, Z-axis focus, halogen lamp/brightness, fluorescence shutter and Port switching.

Video Description:
This video begins with a full front view of the Zeiss Axiovert 200M Inverted Motorized Microscope powered up and running.
The camera pans from right to left for better detailed viewing. Next, you will see the software information for the two programs used (Micromanger and AmScope).
A test is given to the joystick control of the stage, followed by the MicroMangers control of the Zeiss Axiovert 200M Inverted Motorized Microscope.
The fluorescence is turned on and through AmScope you may see how each filter cube effects the visual.
A final front view of the Zeiss Axiovert 200M Inverted Motorized Microscope will end this video.

Datasheet: N/A



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Zeiss Axiovert 200M Inverted Motorized Fluorescence DIC Microscope Pred.Observer

Manufacturer Description and Specification:

The Zeiss Axiovert 200 Inverted Microscope is built for examination of tissue culture flasks, Petri dishes, microtiter plates, etc., in transmitted and reflected light. It can be used for bright field, phase contrast, differential interference contrast, and epi-fluorescence techniques. We use it in our laboratory primarily for microinjection of DNA into the syncytial germ line in the distal gonads of C. elegans in order to generate transgenic worms, and to inject dsRNA to induce RNAi. We have accessories for microinjection connected to this microscope. This microscope has a sturdy stand providing multiple attachment possibilities for micro-manipulation.

Some of the major features of this microscope are: ICS optics for image creation; a very nice LCD display located at the top of instrument (a two line display showing objective position, magnification and contrasting technique, and lamp brightness); an amazing, high quality 25 mm field of view; 6-position nosepiece; 5-position reflector turrent for epifluorescence; 6-position condensor turrent (phase and interference contrast); changeable aperture diaphragm and luminous-field diaphragm sliders in reflected light; fluorescence shutter; and an illuminator (a 100 W Halogen light bulb). Other features include a toggle switch for brightness control of the halogen illuminator, and a focusing drive which consists of a big knob for coarse adjustment and a small knob for fine adjustment.

The microscope stage is very spacious and includes a specimen holder for microscope slides. We have our worms immobilized on cover slips with a thin layer of agarose; the slips sit on top of the slides. Another unique feature of the stage is that it can be rotated 360 degrees, making manipulation very easy without having to actually move the slides themselves. Another unique feature is the Light Manager, which automatically sets the illumination intensity of the halogen illuminator to the most recently saved value for each objective position. Also, when changing objectives, the lamp voltage is reduced automatically to avoid glare.

Manufacturer Documentation:

Boston Industries, Inc. © 2018