Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply

Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply

	Approved Vendor to many US, Canada, and EU Universities
	International Shipping
	Customer References Available
	Training and Installation Options Available

Stock: In Stock
Brand: Life Technologies
Product Code: 49330
Condition: Pre-Owned

$38,995.00

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Description
How we pack

For Sale Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ Autosampler and External Fluid Supply

Fully Functional
Serviced & in Stock
Expert sales consultation
Includes remote installation and instrument training session
Includes subscription to De Novo FCS Express 7 Analysis software
Preventative Maintenance (PM) will be performed before shipping
CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
(r) Installation and instrument operation training is available

Made by Life Technologies, a brand of Thermo Scientific
Setup with (4)Lasers/(14) Colors/(16)Detectors - Max configuration

Here is what you will receive:

Thermo Attune NxT Acoustic Focusing Cytometer AFC2
Attune NxT Gen Autosampler
Attune NxT Extended Fluid Supply System
Desktop PC running Attune NxT Software v5.3 (w/ license dongle, monitor, mouse and keyboard)
Complimentary De Novo FCS Express analysis software (1-Year Subscription) RUO version (Recommended for use with Attune NXT)
Communication and power cables

When you purchase a flow cytometer from Boston Industries, you also receive a complimentary 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
With FCS Express 7, you will have the latest Research Use analysis software.

Thermo Attune NxT Acoustic Focusing Cytometer Configuration:

Optics:(4) Lasers, (14) Colors, (16) Detectors

Forward Scatter Channel: 488/10 blue laser
Side Scatter Channel: 488/10 blue laser
405nm violet laser: 440/50, 512/25, 603/48, 710/50
488nm blue laser: 530/30, 590/40, 695/40
561nm yellow laser: 585/16, 620/15, 695/40, 780/60
637nm red laser: 670/14, 720/30, 780/60

Flow Rate: 12.5 to 1,000 uL/min
Sample Analysis Volume: 20ul - 4mL
Particle Size Range: 0.5 - 50um
Data Acquisition Speed: up to 35,000 events/second with 34 parameters, based on a 10% coincidence rate per Poisson statistics
Maximum Electronic Speed: 65,000 events/second with all parameters
No-wash, no-lyse sample preparation workflow
Cycle time: 20-40 min for tube

Attune NxT Autosampler is compatible with many different plate formats, including 96-well, 384-well, and deep-well plates
<42 min for 96-well plate; <180 min for 384-well plate

Attune NxT External Fluid Supply (EFS) is an accessory for the Acoustic Focusing Cytometer that provides increased focusing fluid and waste capacity, allowing the processing of multiple samples without the need to replenish the focusing fluid supply or empty the waste container.
The extended capacity of the system with the Attune NxT EFS installed is 10 L for focusing fluid supply and up to 18 L for the waste volume (including 2 L of bleach for biosafety requirements)
Electrical input: from Attune NxT Acoustic Focusing Cytometer

Customization/Options: N/A

Testing performed by our in-house service team:

The Thermo Attune NxT Acoustic Focusing Cytometer passed all tests and was found to be fully functional:

We checked the startup and initialization process.
We connected The Thermo Attune NxT Acoustic Focusing Cytometer to the software.
We tested the Attune NxT Sample Pump
We tested Attune NxT Acoustics
We ran fluidics priming and startup sequences.
We conducted several cleaning cycles, including a long cleaning cycle and a flow cell cleaning.
We tested the functionality of the Auto Sampler.
We performed a baseline test with Attune Performance beads.
We checked its performance with Attune Performance beads.
We confirmed external fluid supply communication
We tested external fluid supply cart pumps and level sensors

Instrument Overview:

The Thermo Attune NxT Acoustic Focusing Cytometer integrates 4 lasers, 14 colors, and 16 detectors for comprehensive signal analysis.
The included autosampler and external fluid supply system facilitate automated sample handling and ensure uninterrupted operation.
This advanced cytometer is designed for efficient flow cytometry analysis, making it ideal for research applications requiring high-throughput capabilities.
The instrument's precise detection and versatile functionality enable ease of detailed analysis of complex cell populations.
Through its advanced features and automation, this cytometer accelerates research processes and enhances data accuracy.

Instrument Application:

Cell sorting and analysis in immunology research
Gene expression profiling in molecular biology
Detection of Rare Cell Populations in Oncology Studies
Multiplexed protein expression analysis in drug discovery
Characterization of circulating tumor cells in cancer research
Immunophenotyping of immune cell subsets in vaccine development

The key features:

4 lasers for diverse analysis
14 colors for comprehensive data
16 detectors for high sensitivity
Autosampler for efficient workflow
External fluid supply for continuous operation
Precise and reliable results

De Novo FCS Express Software Features

Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

Benefits of the Complimentary DeNovo FCS Express Software

Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
Flexible Visualization Options: A wide range of plotting options helps visualize data effectively, facilitating better understanding and presentation.
Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

Additional technical info:

Analysis Software: FCS Express Analysis Software
Datasheet: Thermo Attune NxT Acoustic Focusing Cytometer Datasheet
Brochure: Thermo Attune NxT Acoustic Focusing Cytometer Brochure
Brochure: Thermo Attune NxT Acoustic Focusing Cytometer Brochure
Manual: Thermo Attune NxT Acoustic Focusing Cytometer Manual
Software Manual: Thermo Attune NxT Acoustic Focusing Cytometer Software Manual
Manual: Attune NxT Gen Autosampler Manual
Manual: Attune NxT Extended Fluid Supply Manual

Note: Subscription is for the Research Use Only (RUO) version.

Video Overview:

About us

How we pack

Why do so many customers choose to order from us?

We have experience servicing and repairing these instruments.
This instrument goes through all the described service steps before shipping. see questions section below
We offer detailed photos and videos of the instruments you buy.
We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
Technical sales consultation available
Remote installation and instrument operation training may be available.
On-site installation may be available in the Boston Area (New England area)
US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Warranty
You get after-sales technical support
Customer references are available
The instrument will be fully functional!
Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

*SM* Small Crate ESQ
200#

COMMON QUESTIONS AND ANSWERS BELOW

What steps do you take to verify, service, and confirm the functionality of the Thermo Attune NxT Acoustic Focusing Cytometer?

We verify various instrument functions individually and together, performing necessary repairs or maintenance to bring the instrument to our quality standards.

We checked the startup and initialization process.
We connected The Thermo Attune NxT Acoustic Focusing Cytometer to the software.
We tested the Attune NxT Sample Pump
We tested Attune NxT Acoustics
We ran fluidics priming and startup sequences.
We conducted several cleaning cycles, including a long cleaning cycle and a flow cell cleaning.
We tested the functionality of the Auto Sampler.
We performed a baseline test with Attune Performance beads.
We checked its performance with Attune Performance beads.
We confirmed external fluid supply communication
We tested external fluid supply cart pumps and level sensors

What supplies do I need?

You will need the consumables starting out:

Can I view the Thermo Attune NxT Acoustic Focusing Cytometer in person at your facility or do a live Zoom demo before it is shipped to me?

We invite you to visit our facility for an on-site demo, or you can request a virtual Zoom demo of the instrument you are interested in.

What about the warranty? Installation, training, maintenance?

Warranty Terms:

The instrument comes fully warranted for both parts and labor.
The customer should retain all packing materials during the warranty period.

What should I watch out for when buying a Thermo Attune NxT Acoustic Focusing Cytometer used/pre-owned from another lab?

Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
Confirm that there is no physical damage or any misaligned internal components.
Ask what accessories are included.
Ask how long the warranty is and what it covers.

Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.

We checked the startup and initialization process.
We connected The Thermo Attune NxT Acoustic Focusing Cytometer to the software.
We tested Attune NxT Sample Pump
We tested Attune NxT Acoustics
We ran fluidics priming and startup sequences.
We conducted several cleaning cycles including long clean and flow cell clean.
We tested the functionality of the Auto Sampler.
We performed a baseline test with Attune performance beads.
We checked its performance with Attune performance beads.
We confirmed external fluid supply communication
We tested external fluid supply cart pumps and level sensors

We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate its condition and performance before you consider purchasing it.

I found it elsewhere for less. What goes into your price?

We acknowledge that sometimes you can purchase this Thermo Attune NxT Acoustic Focusing Cytometer elsewhere, possibly for less.

Here are some of the reasons for our price.

We may offer longer warranty terms.
We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
We need technical staff that can perform testing/repairs and provide after-sales support if there are any issues.
We invest considerable time into the testing and service of each instrument we sell.
We have to purchase various testing supplies (often expensive) to perform testing.
We must purchase any required or missing accessories to complete the instruments.
We have to procure and stock service parts to support our warranty.
We have extensive experience packing and shipping sensitive instruments.
We guarantee that the instrument will be functional.

This procurement is for a University or College. Are purchase orders with NET terms accepted?

If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
POs are accepted from US and Canadian universities.

How fast will you ship?

We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Will my instrument be insured during shipping?

All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
If the buyer handles shipping, the responsibility for shipping insurance falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.

How do you pack? Have you previously packed and shipped Thermo Attune NxT Acoustic Focusing Cytometer? Do you ship Internationally?

Packing

Smaller instruments ship via UPS Parcel Delivery with adequate packing material and a strong box. Or, when combined with other orders, it may be packed in a crate.
Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
Accessories are packed safely in boxes with padding and then safely packed inside the crate.
We may not use a crate for local deliveries to save you money *if delivered by us.
We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
Photos are taken during the packing process to make sure nothing is missed.

USA/Canada Shipping

Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.

International Shipping

Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.

Our experience and process

We have safely packed and shipped thousands of sensitive analytical instruments.

How much will I save buying it pre-owned, and is it worth it?

A lot, and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.

You will pay less than half the cost of buying a new instrument. Why not spend the leftover funds on another instrument for your lab?
You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
Know that your instrument was tested and working the day it left our facility and that it is covered if there are faults.
Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.

Sustainability

Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO² output, and water use that would be used during the manufacturing process.

I have more questions; I want to receive a formal quote.

Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

OPEN THE FORM

We understand that every instrument has unique packing and shipping requirements; our team understands that no one wants to spend thousands of dollars and receive a damaged instrument.

Our process involves evaluating every instrument to determine the safest shipping method and the best process for packing.

We generally ship parcels either via UPS or freight for all larger goods.

Whenever we ship freight, we use an ISPM-15 wooden crates or pallets made from ½” plywood and 1x4 framing. (Our crates are Internationally Compliant).

On freight shipments, we use 2” 1.7 lb. density foam, which provides excellent cushioning and support for the instrument.

Instruments are safely braced inside the crates. We use solid boards with 1” foam shrink-wrapped around them, which provides cushioning in all directions and holds instruments firmly in place.

Final step is to photograph everything inside the crate and then seal it with screws.