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![]() | Approved Vendor to many US, Canada, and EU Universities |
![]() | International Shipping |
![]() | Customer References Available |
![]() | Training and Installation Options Available |
- Stock: In Stock
- Brand: Sartorius Stedim
- Product Code: 65533
- Condition: Pre-Owned
For Sale Sartorius Stedim Biostat B Bioreactor Twin for Cell Culture w/ 5L & 10L SW Glass Univessels
- Fully Functional
- Serviced & in Stock
- Current Model
- Good cosmetic condition
- Technical sales consultation available
- CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
- Includes remote instrument setup and training session
Here is what you will receive:
- Sartorius Biostat B control unit Ref.: BB-8821111
- (2x) Direct drive agitation motor w/ attached cables
- (1x) 10L SW Glass Univessel w/ 2x pitched impellers
- (1x) 5L SW Glass Univessel w/ 2x pitched impellers
- (2x) Temp sensor w/ cable
- (1x) Unused pO2 - Sensor, 425/12-vp-hm-Optical
- (1x) Unused pH-Sensor, 425/12-vp-MT
- (1x) Unused pO2 - Sensor, 325/12-vp-hm-Optical
- (1x) Unused pH-Sensor, 325/12-vp-MT
- (2x) pH sensor cable
- (2x) O2 sensor cable
- (1x) Heating blanket 435W
- (1x) Heating blanket 780W
- (2x) Exhaust coolers
- (2x) Drain/Cleaning tool
- Required power and communication cables
We have other Biostat B controller configurations that may better suit your application.
Sartorius Stedim Biostat B Bioreactor Controller Configuration for Both Sides:
Gas flow supply connections
| Gas flow control | 2x3 TMFCs (Thermal Mass Flow Controller), Precision: ±1% full scale; 2x5 Flow meters; Precision: ±5% full scale |
| Gas mixing option | 4-Gas mixing (Air, O2, N2, CO2) with sparger and headspace outlet (Air, CO2) With additional valve Air | CO2 mixture can be created in the overlay line to reproduce conditions set in a CO2 incubator. |
| (Gas-in) Supply ports | Air, O2, N2, CO2; Maximum 1.5 barg |
| (Gas-out) Sparge connection | 5L SW Univessel: Air - 300 ccm (MFC) O2 - 100 ccm (MFC) N2 - 300 ccm (Solenoid-Controlled Rotameter) CO2 - 100 ccm (MFC)
10L SW Univessel: Air - 500 ccm (MFC) O2 - 300 ccm (MFC) N2 - 500 ccm (Solenoid-Controlled Rotameter) CO2 - 300 ccm (MFC) |
| (Gas-out) Overlay connection | 5L SW Univessel: Air overlay - 1.5 Ipm (Solenoid-Controlled Rotameter) 10L SW Univessel: Air overlay - 8 Ipm (Solenoid-Controlled Rotameter) |
Controller peristaltic pumps
| Built-in Pumps: | 2x2 Watson Marlow 114, fixed speed (on|off controlled) pumps for feed | base |
SW (Single-wall) Glass Univessel
| Working volume range: | 5L SW Univessel: 0.6–5L 10L SW Univessel: 1.5 | 5– 10L |
| Mixing: | 2 x pitched impellers per vessel |
| Aeration: | L-sparger |
Motor drive
| Drive unit: | 200 W motor for 1L–10L Vessel |
| Stirrer shaft coupling: | Direct Coupling |
| Agitation ran ge with direct drive: | 5 L: 20– 1,500 rpm 10 L: 20–800 rpm |
Temperature Control Module
| Temperature control for Univessel Glass SW Vessels | External heating blanket and cooling finger(Sold Separately) |
| Temperature control range: | 8 DegC above cooling-water inlet temperature up to 60 DegC |
| Heating capacity: | 435 W for 5L, 780 W for 10L |
Controller digital connections/inputs (Click here to expand this section)
| Port Label | Typical Accessory / Sensor |
|---|---|
| Serial-A Scales connection(FWEIGHT), RS-232, M12 plug | Balance/culture vessel weight sensor (e.g., 60 kg/300 kg max) |
| Serial-B Scales connection (FWEIGHT /VWEIGHT), RS-232, M12 plug. | Substrate weight or vessel load cell for gravimetric feed control* |
| Serial-C UniVessel® SU Holder, BioPAT® ViaMass, RS-485, M12 plug | BioPAT ViaMass inline biomass sensor for SU vessels* |
| Temp Temperature sensor, M12 plug | Pt100 temperature sensor (0–150 °C, ±0.1 °C accuracy) |
| Thermostat (x2) Heating/cooling control return and inlet | |
| Heating blanket control inlet | Electrical heating connection to heating blanket for Univessel Glass Single-wall Culture Vessels |
| pH/Redox-A pH sensor or Redox sensor input, VP8 plug | Combined pH/Redox sensor (e.g., Mettler Toledo InPro series) |
| pO₂-A pO2 sensor input, VP8 plug | Optical DO sensor (e.g., VisiFerm DO, Mettler InPro 6800) |
| Exhaust (x2) Exhaust cooler control inlet/return | Cooling water loop for glass/single-use exhaust condenser |
| Sparger Aeration control | Sparger line for Air, O2, N2, CO2; (with Flow meter or MFC control) |
| Overlay Aeration control | Headspace line for Air (with Flow meter or MFC control) |
| Pump-B / Pump-C External peri-pumps for feed/acid/base/antifoam addition, M12 plug | Watson-Marlow 120 series pumps (speed-controlled, external)* |
| External Signal Input A/B (M12 plug) | Allows integration of analog signal inputs (0–10 V / 4–20 mA). |
| External Signal Input C/D (M12 plug) | Additional analog signal inputs for extended control or monitoring. |
| Stirrer (x2) Stirring motor drive and encoder feedback | Direct drive or magnetic coupling motors (e.g., Sartorius 200 W) |
(* Depending on the configuration of the device, the port may be present but not configured for operation. Configuration changes must be made by the manufacturer.)
Controller Weight/Dimensions/Power Requirements
| Power requirements | 120 V (±10%),60 Hz, max. power consumption 12 A |
| Dimensions (W x D x H) | 35×82.2×43 cm (14 ×32 × 17 in) |
| Net weight: | 55 kg (121 lb) |
Please note that, depending on the requested options, we may recommend a different bioreactor model that better suits your application.
Customization/Options: (Please inquire for available inventory)
- Stirrer options
- Vessel scale options
- Source scale options
- Exhaust analyzers
- Cooling water circuit for the exhaust cooler
Testing performed by our in-house service team:
The Sartorius Stedim Biostat B Bioreactor Controller passed all tests and was found to be fully functional:
- We verified system startup and confirmed successful initialization of all core modules.
- We inspected and tested the control panel, display, and interface for responsiveness and accuracy.
- We verified the pH and DO probes using standard buffer solutions and ensured accurate readings.
- We tested the temperature and agitation control systems for proper regulation and stability.
- We evaluated the gas flow control, overlay, and sparging functions to confirm consistent delivery and response.
- We tested the pump's medium-addition and base-delivery functions.
- We reviewed alarm functions and safety interlocks to ensure the system responds appropriately.
Instrument Overview:
- Sartorius Stedim Biostat B-CC Twin is designed for controlled cell culture operations at multiple working volumes.
- Dual-control architecture enables independent monitoring and regulation of two SW glass cultivation vessels.
- Compact design with integrated control for pH, DO, temperature, agitation, and gas flow in each vessel.
- Heating blankets, exhaust coolers, and temperature probes maintain stable culture conditions during operation.
- Biostat B-CC Twin is ideal for batch, fed-batch, and perfusion processes in benchtop settings.
Instrument Application:
- Mammalian and insect cell culture under controlled conditions
- Seed train development and scale-up studies
- Fed-batch and batch cell culture process development
- Process optimization for suspension cell lines
- Media and feed strategy evaluation
- Metabolic and growth kinetics studies
- Small-scale bioproduction runs
The Key Features:
- Twin control unit for parallel cultures
- Supports 5 L and 10 L SW glass vessels
- Precise control of pH, DO, and temperature
- Gentle agitation for shear-sensitive cells
- 4-gas mixing with precise MFC control
- Integrated fixed-speed peristaltic pumps
- Compact design with touch screen display
Additional technical info:
Video Overview:
About us

How we pack

VIEW SOME OF THE CUSTOMERS WE HELPED HERE
Why do so many customers choose to order from us?

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
COMMON QUESTIONS AND ANSWERS BELOW
What are the steps you take to verify, service, and confirm the functionality of the Biostat B-CC Twin Bioreactor Controller?
We verify instrument functions individually and in combination, performing necessary repairs or maintenance to meet our quality standards.
- We verified system startup and confirmed successful initialization of all core modules.
- We inspected and tested the control panel, display, and interface for responsiveness and accuracy.
- We verified the pH and DO probes using standard buffer solutions and ensured accurate readings.
- We tested the temperature and agitation control systems for proper regulation and stability.
- We evaluated the gas flow control, overlay, and sparging functions to confirm consistent delivery and response.
- We tested the pump's medium-addition and base-delivery functions.
- We reviewed alarm functions and safety interlocks to ensure the system responds appropriately.
What supplies do I need?
You will need the consumables starting out:
Can I view the Biostat B-CC Twin Bioreactor Controller in person at your facility or do a live Zoom demo before it is shipped to me?
We invite you to visit our facility for an on-site demo or feel free to request a virtual Zoom demo of the instrument you are interested in.
What about the warranty? Installation, training, maintenance?
Warranty Terms:
- The instrument comes fully warranted for both parts and labor.
- The customer should retain all packing materials during the warranty period.
What should I watch out for when buying a Biostat B-CC Twin Bioreactor Controller used/pre-owned from another lab?
- Make sure the instrument is properly packed for shipping and will arrive safely. If the instrument is large, request to have it packed in a wooden crate.
- Confirm that there is no physical damage or any misaligned internal components.
- Ask what accessories are included.
- Ask how long the warranty is and what it covers.
Here is what our technical team tests so you can request something similar and ensure your instrument will be fully functional.
- We verified system startup and confirmed successful initialization of all core modules.
- We inspected and tested the control panel, display, and interface for responsiveness and accuracy.
- We verified the pH and DO probes using standard buffer solutions and ensured accurate readings.
- We tested the temperature and agitation control systems for proper regulation and stability.
- We evaluated the gas flow control, overlay, and sparging functions to confirm consistent delivery and response.
- We tested the pump's medium-addition and base-delivery functions.
- We reviewed alarm functions and safety interlocks to ensure the system responds appropriately.
We recommend inspecting the instrument in person or requesting a virtual demo; this will allow you to evaluate the condition and performance of the instrument you are considering purchasing.
Do you have a Biostat B-CC Twin Bioreactor Controller on hand?
- This is a common model, and we generally stock multiple instruments, accessories, and many replacement parts to back up our warranty.
I found it elsewhere for less. What goes into your price?
We acknowledge that sometimes you can purchase this model instrument elsewhere, possibly for less.
Here are some of the reasons for our price.
- We may offer longer warranty terms.
- We cover shipping costs during the warranty period. (Customer is required to save packing materials for the duration).
- We have to have technical staff that can do the testing/repairs and after-sales support if there are any issues.
- We invest considerable time into the testing and service of each instrument we sell.
- We have to purchase various testing supplies (often expensive) to perform testing.
- We have to purchase any missing required accessories to make the instruments complete.
- We have to procure and stock service parts to support our warranty.
- We have extensive experience packing sensitive instruments. See the shipping section in the website menu for info on how not to pack.
- We guarantee that the instrument will be functional.
This procurement is for a University or College. Are purchase orders with NET terms accepted?
- If you are a US or Canadian customer, we are likely already a registered vendor in your University's vendor system. This would greatly expedite the process of PO approvals and get this instrument into your lab faster.
- POs are accepted from US and Canadian universities.
How fast will you ship?
- We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Will my instrument be insured during shipping?
- All instruments shipped within the USA and internationally are fully insured. This applies as long as we are the shipper and handle shipping arrangements.
- If the buyer handles shipping, then shipping insurance responsibility falls to the buyer; we highly advise that the buyer obtain shipping insurance from their carrier.
How do you pack? Have you packed and shipped Biostat B-CC Twin Bioreactor Controller Instruments in the past? Do you ship Internationally?
Packing
- Most larger instruments ship in fully enclosed ISPM15-certified wooden crates with proper padding underneath, and the sides have moving components braced.
- Accessories are packed safely in boxes with padding and then safely packed inside the crate.
- We may not use a crate for local deliveries to save you money *if delivered by us.
- We often use “tilt’ & “shock” indicators on the crates to ensure the crate has not been excessively tilted and dropped where equipment may suffer damage.
- Photos are taken during the packing process to make sure nothing is missed.
USA/Canada Shipping
- Within the US and Canada, we will ship via selected freight carriers. We do not use low-price/high-damage carriers as we want the equipment to get safely to you.
International Shipping
- Internationally, we will only ship via Air Freight or Air Parcel (such as UPS or DHL), which provides reliability, no exposure to Ocean weather, and timely delivery.
We have extensive experience packing and shipping fragile instruments globally.
Our experience and process
- We have safely packed and shipped thousands of sensitive analytical instruments.
How much will I save buying it pre-owned, and is it worth it?
A lot…and it is undoubtedly worth it, and here is why. Feel free to ask for feedback from hundreds of customers who bought from us.
- You will pay less than half vs. buying a new instrument. Why not spend leftover funds on another instrument for your lab?
- You will get our standard warranty, plus, when available, an option to obtain an extended warranty agreement.
- Know that your instrument was tested and working the day it left our facility and is covered if there are faults.
- Get extra peace of mind by adding an extended warranty or service agreement when such an option is available.
Sustainability
- Well, this one is easy: buying a pre-owned instrument is great for the planet; you are reducing unnecessary waste, CO2 output, and water use that would be used during the manufacturing process.
I have more questions; I want to receive a formal quote.
Absolutely! Please give us a call (1-617-397-3486) or complete this form to get your questions answered or receive a formal Quote.

