- Stock: SOLD
- Brand: Nikon
- Product Code: 19078
Here is a Nikon Ti-E PFS Perfect Focus Inverted Fluorescence DIC Phase Contrast Microscope
Setup for Brightfield with Fluorescence, DIC and Phase Contrast studies.
This microscope system is optimized for biological studies that require enhanced contrasting options as well as prolonged sample observation stability.
The Nikon Perfect Focus System (PFS) allows observation stability regardless of axial focus fluctuations.
The PFS system provides real time correction of focus drift due to environmental variants within multiple magnifications and contrasting options.
The LEP 99S008 motorized stage has a travel range of 121 x 102mm. The custom stage insert allows for slide, petri dish, or wellplate.
The TI-RCP touchscreen controller allows for simple and intuitive control of a large array of custom configurations and allows for simple and efficient switching of base functions, all at the touch of your fingertips.
The Lumencor Sola Light Engine features bright and efficient (5) LED illumination with a near limitless lifespan, eliminating the need for bulb replacement.
All motorized base controls and MU1403 camera settings are able to be controlled and automated virtually within the provided MicroManager software.
This Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope is in good working order with all aspects tested and working fully.
Microscope optics have been cleaned professionally under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source fully: PASSED
Tested LED lamp and light guide: PASSED
Tested motorized condenser and prisms fully: PASSED
Tested motorized filter cube turret fully: PASSED
Tested motorized focal adjustment to limits: PASSED
Tested motorized XY stage to limits: PASSED
Tested all DIC components fully: PASSED
Tested eyepieces for clarity: PASSED
Tested objectives for clarity: PASSED
Tested touchpad control fully: PASSED
Tested motorized port splitter to split between all ports: PASSED
Tested motorized shutters and light adjustments: PASSED
Tested all filters and prisms: PASSED
Tested display screen for dead pixels: PASSED
Tested joystick stage control: PASSED
Tested communication to software for virtual control: PASSED
Tested live feed capabilities: PASSED
Tested PFS perfect focus capabilities: PASSED
Tested all motorized control of microscope base: PASSED
During testing this Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the motorized components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse Ti-E Inverted Microscope
TI-HUBC/A Power Supply
TI-RCP Touchscreen Controller
TI-TERG Binocular Head
(2) CFI 10x/22 Eyepieces
Prisms / Condensers:
TI-CT-E 5-position Motorized DIC Condenser Head LWD 0.52
Prisms: PhL, DIC N2 (#DIC0273)
TI-DH Illumination Tower
TI-FL Fluorescence Light Path
TI-FLC-E 6-position Motorized Filter Cube Turret
DAPI (blue) EX:395/25 DM: EM:460/50 (#F00541)
FITC (Green) EX:465-495 DM:505 EM:515-555 (#F00447)
CY5 (red) EX:620/60 EM:660 EM:700/75 (#F00581)
10x/0.30 Plan Fluor DIC L/N1
40x/0.75 Plan Fluor DIC M/N2
DIC N2 Condenser Prism
hP Polarizer (#DIC254)
T-A2 Analyzer (#DIC290)
D-LH/LC 12V 100W Halogen Lamp w/ TE2-PS100W power supply
SOLA light engine LED Lamp w/ EXFO culminating adapter and liquid light guide
LEP Motorized XY Stage
LEP MAC6000 Controller
TI-ND6-PFS 6-position Motorized Nosepiece
4x/0.13 Plan Fluor PhL DL (#O2369)
10x/0.30 Plan Fluor DIC L/N1 (#O0612)
20x/0.75 CFI Plan Apo VC (#O2009) (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
40x/0.75 Plan Fluor DIC M/N2 (#O1747)
Photo / Port Slits:
Left 100% or Left 80% Head 20% or Right 100% or Head 100%
AmScope MU1403 14MP USB 3.0 Color CMOS C-Mount Microscope Camera
Computer and Software:
Dell Latitude i5 running AmScope software and MicroManager software
XY Stage, Z-Axis, Light Path switching, Nosepiece, Condenser, Fluorescence turret
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield, Fluorescence, Phase Contrast, DIC
Objectives: 4x/0.13 Plan Fluor PhL DL, 10x/0.30 Plan Fluor DIC L/N1, 20x/0.75 CFI Plan Apo VC, 40x/0.75 Plan Fluor DIC M/N2
Light Sources: 12V 100W Halogen Lamp, SOLA LED Light engine
Stage: Motorized XY
This Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope is currently set up for Brightfield with Fluorescence, Phase Contrast and DIC studies.
AmScope MU1403 14MP Digital Camera is capable of taking detailed images.
With 4x, 10x, 20x, 40x objectives along side 10x eyepieces the total magnification of this scope is 400x
The video starts with a wide front view of the Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope.
After that camera pans closer to see each component of the Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope system in details.
Then we observe functions of the Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope including all options.
The video ends with a final front view of the Nikon Ti-E PFS Fluorescence Motorized DIC Phase Contrast Microscope.
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
Please do not hesitate to contact us if you have any questions.
Here we are showing you our standard packing process
1. All items are different, but they all follow the same principle when packaging.
2. Start with a well-constructed crate
A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.
3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.
4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.
5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.
6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.
7. Final step is to photograph everything inside the crate and then seal it with screws.
All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to firstname.lastname@example.org or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.