- Stock: In Stock
- Brand: Nikon
- Product Code: 24988
Here is a Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope Pred Ti
Setup for Brightfield, Fluorescence, Phase Contrast and DIC studies.
This microscope system is optimized for biological studies where enhanced contrasting options are required.
This microscope has a total magnification range of 100x-400x.
The LEP motorized stage has a travel range of 120mm-100mm. The custom stage insert will accommodate slide, petri dish, or well plate.
This Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope is in good working order with all aspects tested and working fully.
Microscope optics have been cleaned professionally by our technician under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source fully: PASSED
Tested fluorescence lamp and source fully: PASSED
Tested transmission halogen light path: PASSED
Tested reflective fluorescence light path: PASSED
Tested objectives for clarity: PASSED
Tested port-splitter to split between camera and eyepieces: PASSED
Tested mechanical condenser: PASSED
Tested mechanical Z-axis: PASSED
Tested mechanical fluorescence turret: PASSED
Tested mechanical objective turret: PASSED
Tested connection from camera to laptop: PASSED
Tested motorized stage virtually and w/joystick: PASSED
Tested live feed for clarity: PASSED
Tested all filter slides: PASSED
Tested polarizer and analyzer: PASSED
During testing this Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse TE300 Inverted Microscope
TE-TL Binoculars w/ mechanical 4-position lens turret (C - Closed, O - Open, M - 2x Magnification, B - Bertrand)
(2) Nikon CFI 10x/22 Eyepieces
Prisms / Condenser:
6-position Mechanical Condenser LWD 0.52
DIC M Prism (#DIC0244)
TE-DH100W Illumination Tower
TE-FM Light Path w/ manual shutters
Nikon D/F/R Triple Band Fluorescence Filter Cube (#642)
Dapi(Blue) EX:340-380 DM:400 EM:435-485
FITC(Green) EX:470/40bp DM:500 EM:515bp
TRITC(Red) EX:540/25 DM:565 EM:620/60
DIC M Prism (#DIC0244)
hP Polarizer (#DIC0212)
hA Analyzer (#DIC0112)
12V 100W Halogen Lamp w/ TE2-PS100W control box
EXFO X-Cite 120 Metal Halide Lamp w/ light guide and culminating adapter
6-position Mechanical Nosepiece
10x/0.30 Plan Fluor Ph1 DL (#O02392)
20x/0.75 CFI Plan Apo VC (#O02016) (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
40x/0.75 Plan Fluor DIC M/N2 (#O02172)
LEP Motorized XY Stage with custom insert
LEP PSSYST Controller
Photo / Port Splits:
20/80 Eye/Front Port
100% Side Port
20/80 Eye/Side Port
AmScope MU1403 14MP CMOS Digital Color Camera
Computer and Software:
Dell Latitude i5 running AmScope software and MicroManager software
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield, Fluorescence, Phase Contrast, DIC
Objectives: 10x/0.30 Plan Fluor Ph1 DL, 20x/0.75 CFI Plan Apo VC, 40x/0.75 Plan Fluor DIC M/N2
Light Sources: 12V 100W Halogen Lamp, EXFO X-Cite 120 Metal Halide Lamp
Stage: Motorized XY
This Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope is currently configured for Brightfield viewing with Fluorescence and Phase Contrast, and DIC studies.
The MU1403 CMOS camera is capable of taking detailed digital images. Dell laptop included for live feed.
With included objectives of 10x, 20x, 40x along side 10x eyepieces the total magnification of the system is 400x
In the first video, we take a look at all the components and accessories on the Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope setup.
We see free and easy movement demonstrated by all moveable components. We finish with a quick look at basic brightfield imagery through the three objectives.
In the second video, we show the effects all brightfield, DIC, phase contrast, and fluorescence components have on the image produced by the Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope, as viewed through the camera live feed.
After all functionality is demonstrated along with it's effect on the image, we take one last look at the entire Nikon TE300 Inverted Fluorescence Motorized Phase Contrast DIC Microscope.
This will ship via freight. Please contact us with your zip code to obtain shipping quote.
Please do not hesitate to contact us if you have any questions.
Here we are showing you our standard packing process
1. All items are different, but they all follow the same principle when packaging.
2. Start with a well-constructed crate
A well-constructed crate would consist of 1/2” to 5/8” Plywood with framing. We generally purchase our crates from a third party who specializes in custom crates.
3. You always want the Instrument to not be directly on the wood deck of the crate. This is done by placing it on foam, inside of the crate. We use 2” 1.7 lb. density foam which works great. It provides cushioning and enough firmness to support the Instrument.
4. The next step is to make sure there is nothing on the deck of the Instrument and the Head/ Arm is secured. In some cases there will be multiple arms to secure but for this example there is only one. The reason for this is so there is no free movement during transit which could cause serious damage to the Instrument.
5. The next step is to make sure your Instrument is covered in either a pallet bag or shrink wrap so no dust or foreign object will get into the crate.
6. The next step is to secure the instrument inside the crate by bracing. We use a method of 2x4’s which have 1” foam shrink wrapped to them so no piece of wood touches the actual instrument.
7. Final step is to photograph everything inside the crate and then seal it with screws.
All equipment warranty request must have a support case opened within the 60-day Domestic USA and 30-day International warranty period.
To open a support case, please send an email to [email protected] or call (508)974-4896.
Please provide your name, contact information, quotation/invoice number and a brief description of the issue that you are experiencing.
Phone and/or Email support will be provided prior to scheduling a return.
Please see the link below for our detailed warranty statement.
Customers must retain all original shipping materials during the warranty period.